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Feti Fatimah
Departement Of Chemistry, Faculty Of Mathematics And Natural Science,Sam Ratulangi University, Manado
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Other

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Journal : jurna beta kimia

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The Effect of Papain Enzyme Concentration on Protein Content and Antioxidant Activity of Skipjack Tuna (Katsuwonus pelamis) Protein Hydrolysate Moleong, Rahel Syallomita; Fatimah, Feti; Kumajas, Jenny
Jurnal Beta Kimia Vol 6 No 1 (2026): Volume 6 Issue 1, May 2026
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jbk.v6i1.27560

Abstract

Protein deficiency is one of the most common nutritional problems found in Indonesia. Skipjack tuna is a source of high-quality protein; however, it is highly perishable and therefore requires processing to extend its shelf life. One alternative processing method is the production of protein hydrolysates, which also possess antioxidant activity; nevertheless, optimization of enzyme concentration is required to obtain protein hydrolysates with optimal characteristics. This study aims to analyze the effect of variations in papain enzyme concentration on protein content and antioxidant activity of skipjack tuna protein hydrolysates, where the hydrolysis process was carried out using papain enzyme concentrations of 4%, 5%, and 6% at a temperature of 55°C for 2 hours, and the research data were statistically analyzed using analysis of variance (ANOVA) to determine differences among treatments. Protein content was determined using the Kjeldahl method, while antioxidant activity was analyzed using the DPPH free radical scavenging assay. The results of protein content analysis showed the best value at an enzyme concentration of 6% at 1.22%, and the results of the antioxidant activity test showed the highest value of 66.04% at an enzyme concentration of 6%; in conclusion, an increase in enzyme concentration affects the increase in protein content and antioxidant activity of the hydrolysate, and these findings indicate that optimizing papain enzyme concentration is important in producing skipjack tuna protein hydrolysates with better functional characteristics, thus having the potential to be developed as a functional food ingredient.
Co-Authors Abdul R.H. Korompot Abdus Salam, Abdus Achmad Hasan Hafidzi Adi Suparwo Agistiani Agustin, Amalia Hikmah Agustin, Feni Dwi Ahmad Izzuddin Aisyiah Restutiningsih Putri Utami Amalia, Zahro Yuli Amaliyatus, Anistiana Anderson Arnold Aloanis Ani Solihat Aniko, Akhmad Iqbal Apriansah, Noval Dias Ariefin, Miftahul Audy Denny Wuntu Aulia, Sweet Nabila Imsaki Aviro, Iftitah Noranza Ayu, Nirendra Dyah Azhad, M. Naely Azhad, Muhammad Naely BARLINA RINDENGAN Bayu Wijayantini, Bayu Budi Santoso Camelia, Camelia Chaleb Paul Maanari Chindy Kalengkongan Damayanti, Fauziah Diansari, Tatit Dinan, Fira Djois Sugiaty Rintjap Dwi Budi Santoso Evi Wahyuni, Evi Fachry Abda El Rahman Fadlilah, Fani Farha, Auva Novia Fatimawali . Fernando Nenabais Fitriani, Diana Novita Frenly Wehantouw Hafidzi, Ahmad Hasan Harianto Harianto Haris Hermawan, Haris Herlambang, Toni I Wayan Gede Suarjana Irene V. Sani, Irene V. Irsyandi, Achmad Iskandar, Ashar Ismanto, Tomi istiqomah istiqomah Izzudin, Ahmad Jakub Saddam Akbar Jane Sulinda Tambas Jefriyanto Ismail Jenny Kumajas Johanis J. Pelealu Johnly A Rorong Johnly A. Rorong Johnly A. Rorong Johnly Alfreds Rorong Johnly Rorong Johnly Rorong Julius Pontoh Jurike Kaunang Korompot, Abdul R.H. Lestari, Endang Putri M, Devy Angreani M. Risal, M. Maharani, Andhika Putra Andryan Maheni Ika Sari Mamondol, Oktavianus Martiana, Eka Martiana, Nina Martini, Ni Nyoman Putu Maryam Z, Amalina Max R.J Runtuwene Maya Sabrina, Adelia Annissa Megawati Nodjeng Meiske S. Sangi Meiske Sangi Mevia, Maulida Feren Arta Miftahul Jannah Molektuzzahro, Arila Moleong, Rahel Syallomita Momuat, Lidya Mongdong, Aldo Mongdong, Aldo G. Montolalu, Gisella T. Muchsin, Reyhan Muhammad Adhimawan Wijaya Muhammad Zaid M. M. Tanaty, Muhammad Zaid M. M. Murtalining Tyas, Wenny Murtaliningtyas, Wenny Musma Rukmana Mustapa Mustapa Nainggolan, Verengki A Nenabais, Fernando Novitasari, Hevi Nugroho, Rifqi Maulana Adi Nuri, Rihadatul Aisylia Nursaid Nursaidah Nursaidah Nursaidah Nursaidah, Nursaidah Nurul Qomariah Obet Obet Oktavia, Erica Bella Oktaviansyah, Renaldy Dwi Pinkan, Aurelia Reghina Pomalingo, Moh Fikri Pratama, Lybel Vega Pratiwi, Ananta Dian Pritantiwi, Sonnia Priyohadoko, Ndaru Tutus Purnomo, Wahyu Agung Adji Puspitadewi, Ira Putri, Septi Lenita Putri, Yulisya Salsabila Qurotul Aini Rachman, Avivatur Rahayu, Jekti Resti, Liana Reynal Maong, Reynal Rhamadanita, Mega Wahyu Ria Yuli Angliawati Ria Yuli Angliawati, Ria Yuli Riliani P. Maradesa RINDENGAN, BARLINA Riris Roisah, Riris Rofi'i, Imam Romadloni, Ririn Indah Rosidah Rosidah Rosma Hani Febrianti Rossytania, Winaldha Ninda Rozzaid, Yusron Rusdiyanto Rusdiyanto Rusdiyanto Rusdiyanto Rusdiyanto, Rusdiyanto Sabtohadi, Joko Sanusi Gugule Saputra, Seno Saputri, Dian Mei Sari, Sinta Anggita Satrio, Ogik Fajar Seftiana, Angelita Sidabukke, Indah U. Br. Siswahyudianto Sodikin, Mohammad Faesal Sofianto, Mohammad Sri Rahayu, Yayu Stefan Marco Rumengan Suci L. Sidik Sukma Hadi, Nadia sulfiana, Icha Sumowo, Seno Suteja , Shintia Noviyanti Suwarno Suwarno Syamsi, Ibnu Darmawan Talcha Kirana Tatit Diansari Reskiputri, Tatit Diansari Tina, Lisa Agus Trina E. Tallei Trina Tallei Tripriyono , Setiyo Budi Tungady, Jessica Tyas, Wenny Murtalining Vanda Kamu Vanda S. Kamu Vanda S. Kamu Verdiyanto, Verdiyanto Wahyu Eko Setianingsih Wardiyah, Silvi Widyabakti, Mega Anjar Wulandari, Aprilia Nadila Wydaswara, Mita Yayu Sri Rahayu, Yayu Sri Yohanes Gunawan Wibowo Yurico, Bagus Zaenuri, Muhammad Mukamal Zahro, Aminatuz