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Journal : Buletin Peternakan

Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency Rony Marsyal Kunda; Slamet Diah Volkandari; Maman Rumanta; Pieter Kakisina
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.58934

Abstract

Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using HaeIII enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequilibrium genetic. It maybe caused limited buck and nonrandom mating in population that effect of low genetic variation. Inbreeding study are needed to explore it. The introgression of bucks from other families in several locations within Lakor island can be an alternative solution to increase the genetic diversity of the lakor goat population.
Co-Authors Achmad Fauzi ahmad yani Alamsyah Alamsyah Amalia Sapriati Amril Latief Anita Erari Anna Ratnaningsih, Anna Anna Retnaningsih Ardiansyah Ardiansyah Aripin, Sopjan Artanti, Yuli Ayi Darmana Bintoro, Sigit Cecep Anwar Hadi Firdos Santosa Daud Woru Deni Kurniawan Deni Kurniawan, Deni Desiana, Yulia Dewi Elfriwaty Br Simamora Elly Elly Endang Wahyuningrum ERNY ROESMININGSIH Esya Anesty Mashudi, Esya Anesty Etty Riani Farrina Veranicha Fredrik Manuhutu, Fredrik Gusti Nurdin Gusti Nurdin Habibi Habibi Hartiningsih Hartiningsih Herawati, Is Eka Hidayah Hidayah Hidayat, Sholeh Hutasoit, Leonard Raden Ida Ayu Putu Sri Widnyani Ika Rizqi Meilya Iryani, Krisna Isti Rokhiyah Javed Octovianus Sipahelut Jayawarsa, A.A. Ketut Jumi Jumi Krisna Iryani Krisna Iryani Krisna Iryani, Krisna Kusumastuti, Mimin Nurjhani Laksono Trisnantoro Latief, Amril Leonard R. Hutasoit Lina Asnamawati Mikdar, Syaeful Mr Amprasto Munir, Ivan Mambaul Nadiarti Nadiarti, Nadiarti Noke Widianingsih Nulhakim, Lukman Ojat Darojat Pieter Kakisina Prasetyarti Utami PUJI LESTARI Puspitasari, Kristanti Ambar Raja Muhammad Sabri Rakhmini Juwita Reskyono, Tahta Resniawati Kurnia Retnaningsih, Anna Riandi Marisa Rif’at Shafwatul Anam Ristasa A., Rusna Rodiyah - Rodiyah Rodiyah Ron Makrony Rony Marsyal Kunda Sabighoot , Yus Alvar Safitri, Dina Sandra Sukmaning Aji Selanno, Frenly M. Sendi Ramdhani Siti Hadianti Siti Julaeha, Siti Siti Kulsum Siti Nurlina Slamet Diah Volkandari Slamet Diah Volkandari, Slamet Diah Sri Dewi Nirmala Sri Sudarwati Supri Supri Teguh Prakoso Tien W. Surjono Tri Wahyuningsih Triandika, Elsa Ucu Rahayu Udan Miharja Ulimawati, Yetti Utami, Yolanda Adelia Uum Suminar Widianingsih, Noke Widiasih Winahyu, Fitria Heri Zaenusolihin