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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Biotropika BULETIN FISIKA Cassowary Jurnal Sewaka Bhakti Widyanatya: Jurnal Pendidikan Agama dan Seni Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika

Ida Bagus Gede Darmayasa

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 268954

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Efforts In Conserving Purebred Bali Cattle As Draught And Beef Type In Bali Island, Indonesia. (UPAYA PELESTARIAN SAPI BALI SEBAGAI TABUNGAN DAN TIPE PEDAGING DI PULAU BALI INDONESIA) I Wayan Kasa; Anak Agung Sagung Sukmaningsih; Ida Bagus Darmayasa
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Bali cattle (Bos javanicus d’Alton) is a part of the complex evolution of all cattle over a longtime in Indonesia. Little is known of the origin of bali cattle in Southeast Asia. The geographicaldistribution of Bos (Bibos) types of cattle suggests that the centre of domestication was Indo-China and Malaysia, then spreading to Bali. Due to such several unique characters of bali cattle, someefforts have been conducting with main purpose to conserve purebred, draught as well as meat typecattle on Bali in particular and Indonesia in general. Method has been employed in this study areliterature explore, visiting, interview with questionnaire sheet. Set of efforts have been conductingin order to conserve purebred bali cattle by the government. For examples, establishment of balicattle Breeding centre of Pulukan village, establishment of bali cattle Breeding centre of Sobanganvillage, establishment of bali cattle Conservation centre of Nusa Penida Island, essense of UdayanaUniversity, Bali and role of Department of Animal Husbandry. It could be concluded thatgovernment as a whole have played an important role in conserving the purebred bali cattle asdraught and meat type cattle at certain suitable places in Bali to fulfill local and national daily meatrequirement by establishing good collaboration with related government agency as well as farmers.

DAYA HAMBAT EKSTRAK KULIT DAUN LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 25923DAN Escherichia coli ATCC 25922 Ariyanti Ni Kadek; Darmayasa Ida Bagus Gede; Sudirga Sang Ketut
Jurnal Biologi Udayana Vol 16 No 1 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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This research aimed to determine the inhibited effect of Aloe (Aloe barbadensis Miller) rind extract to the growthof bacteria Staphylococcus aureus and Escherichia coli and the most effective concentration for the resistanceagainst both of bacteria tested. The results showed that the extracts of Aloe rind inhibited the growth of bacteriaStaphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. It was indicated by the formation ofinhibition zones with the largest diameter average occurred concentrations of 100% with the size 11,58 mm forthe Staphylococcus aureus and 6,81 mm in Escherichia coli. The highest concentration inhibited the growth ofStaphylococcus aureus ATCC 25923 was 100% and 75% for the Escherichia coli ATCC 25922.

UJI KEBERADAAN DAN KARAKTERISASI MIKROBA PELARUT FOSFAT PADA BERBAGAI MERK PUPUK ORGANIK Ni Luh Putu Eka Suartini; I.B.G. Darmayasa; I.P.G. Ardhana
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Penelitian ini bertujuan untuk menguji keberadaan, mengetahui total mikroba dan karakterisasi mikroba pelarut fosfat pada berbagai merek pupuk organik. Keberadaan mikroba pelarut fosfat dicirikan dengan terbentuknya zona bening pada media pikovskaya, dengan rata-rata total mikroba pelarut fosfat terbanyak terdapat pada Pupuk Botani (Pubotan) Tanah Subur dengan total mikroba 99,93 x 105 (CFU/g pupuk) sedangkan yang paling sedikit terdapat pada Pupuk Organik Kompos (Limbah Sapi) dengan total mikrobanya yaitu 33 x 105 (CFU/g pupuk). Isolat bakteri pelarut fosfat yang ditemukan yaitu 7 isolat dengan 3 isolat bakteri tergolong genus Klebsiella dan 4 isolat bakteri tergolong genus Enterobacter. Sedangkan koloni cendawan pelarut fosfat yang ditemukan yaitu 5 koloni cendawan pelarut fosfat dengan 3 koloni tergolong Aspergillus niger dan 2 koloni tergolong Aspergillus flavus.

Potensi Rhizopus spp. dalam mengendalikan pertumbuhan Aspergillus flavus FNCC6109 pada pakan konsentrat ayam broiler Lalu Muhammad Sakti Surya Jagat; Ida Bagus Gede Darmayasa; I Made Sara Wijana
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p06

Aspergillus flavus contamination of agriculture in Indonesia can cause problems to animal health and productivity. Some factors can support the appearance of contamination in feed, especially temperature and humidity. The main objective of this research was to investigate potency of Rhizopus spp. on inhibit A. flavus FNCC6109 in broiler chicken concentrate feed. The experiments were conducted dual culture method and the inhibition test of the Rhizopus spp. filtrate culture was incubated for 3, 4 and 5 days on in vitro. The in vivo test was directly applied in broiler chicken concentrate feed which added Rhizopus spp. filtrate culture concentration at 10% (v/v), 20% (v/v), 30% (v/v), 40% (v/v), dan 50% (v/v). The results showed that the Rhizopus spp. filtrate culture significantly (P?0,05) to inhibit the growth of A. flavus FNCC6109 both in vitro and in vivo. The percentage inhibition of Rhizopus spp. filtrate culture incubated for 5 days showed 67,47±2,10% relatively better results than 3 and 4 days, and therefore was used in the in vivo. Application of 50% (v/v) Rhizopus spp. filtrate culture to the broiler chicken concentrate feed significantly reduced 82% population of A. flavus FNCC6109 after 15 days incubated relative to that of negative control (concentrate feed without addition Rhizopus spp. filtrate culture and A. flavus FNCC6109).

SKRENING BAKTERI TOLERAN PESTISIDA DENGAN BAHAN AKTIF KLORANTRANILIPROL ASAL TANAH PERTANIAN BATURITI TABANAN BALI NI KADEK WIWIK SINTA DEWI; IDA BAGUS GEDE DARMAYASA; I KETUT SUNDRA
Jurnal Biologi Udayana Vol 21 No 1 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

In Indonesia agriculture practice often used the large scale pesticide application such as insecticide, herbicide, and fungicide. The wide use of toxic pesticide has created numerous problem in increasing environtmental hazard to human and to other animals. Many of soil bacteria had important role to degrading chemical compounds into simpler compounds as a bioremediation agent. The aim of this study was to screen the Chlorantraniliprole tolerant bacteria using soil mineral medium with Prevathon pesticide addition, also teo identificate the species of bacteria. This research was conducted at Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Udayana University. The research was done in three analysis, (1) bacteria test on Prevathon pesticide addition to mineral medium treatment, (2) characterization of bacteria, (3) Identification of pesticide tolerant bacteria with BD BBL Crystal Enteric/Non FermenterID Kit. The results showed that mineral medium with the addition of pesticides Prevathon treatment able to provide a significant different effect on the enrichment stage 1, stage 2 and stage 3 (P<0.05), there was 5 isolates pesticide tolerant bacteria that isolated from Baturiti Tabanan cultivated soil that was BSP 1, BSP 2, BSP 3 known as gram negative bacteria, and BSP 4, BSP 5 known as Gram positive bacteria, pesticide tolerant bacteria identified as Serratia marcescens which is a Gram negative bacteria group and may cause pathogenic.

DAYA HAMBAT FRAKSINASI EKSTRAK SEMBUNG DELAN (SPHAERANTUS INDICUS L) TERHADAP BAKTERI ESCHERICIA COLI DAN STAPHYLOCOCCUS AUREUS IDA BAGUS GEDE DARMAYASA
Jurnal Biologi Udayana Vol 12 No 2 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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A research entitled "Inhibitid activity of plant fractination extract of Sembung Delan (Sphaeranthus indicus L.) to Eschericia coli and Staphylococus aureus bacterial was carried aut to find the active of plant extract that responsible for bactericidal agent. This research used absorbance disc method to determine inhibitid fractionation extratc of Sembung Delan to growth of Eschericia coli and Staphylococus aureus bacterial. Results of this study showed that the crude extract of Sembung Delan inhibited the growth of Eschericia coli and Staphylococus aureus on Muller- Hinton medium, indicated by the formation of inhibition zone around the disc loaded with plant extract at concentration of 10,000 ppm, 20,000 ppm, 30,000 ppm, 40,000 ppm, and 50,000 ppm, with diamtre of 10mm, llmm, 12mm, 13mm and 16 mm respectively to S. aureus, forE. coli crude extract at concentration of 10,000 ppm, 20,000 ppm, 30,000 ppm, 40,000 ppm, and 50,000 ppm using diametre each of 12 mm, 13 mm, 15 mm, 17 mm and 19 mm respectively . From 17 fractions obtained from fractionation, fraction No. Vm (eluted with ethyl acetate:hexan) with Rf value of 0,9 ; 0,8 ; 0,7 ; 0,68 ; and 0,62, fraction No. DC (eluted with etihil acetate : hexan) with Rf value of : 0,98, ; 0,94 ; and 0,82, fraction No. XV (eluted with ethyl acetate 100%) Rf value of : 0.03; 0.23; 0.35 and fraction No. XVI (eluted with ethyl acetate 100%) with Rf value of: 0.03; 0.14; 0.17; 0.21, effectivity inhibited the growth of test bacterial.

SEMBUNG DELAN (Sphaerantus indicus L.) TERHADAP Pseudomonas solanacearum L. PENYEBAB PENYAKIT LAYU PADA TANAMAN TOMAT Ida Bagus Gede Darmayasa
Bumi Lestari Journal of Environment Vol 6 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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A research entitled “Inhibitid activity of plant fractination extract of Sembung Delan(Sphaeranthus indicus L.) to the causal agent of bacterial wilt on tomato” was carried out to find outthe active fraction of plant extract that responsible for bactericidal activity againt P. solanacearum L.,Results of this study showed that the crude extract of S. indicus inhibited the growth of P.solanacearum L. on PDA. It was indicated by the formation of inhibition zone around the disc loadedwith plant extract at concentration of 0 ppm, 10,000 ppm, 20,000 ppm, 30,000 ppm, 40,000 ppm, and50,000 ppm. From 17 fractions obtained from fractionation, fraction No. XV (eluted with ethylacetate 100%) contains three compunds with Rf value: 0.03; 0.23; 0.35 and fraction No. XVI (elutedwith ethyl acetate 100%) contains four compunds with Rf value: 0.03; 0.14; 0.17; 0.21, effectivityinhibited the growth of P. solanacearum L

ISOLASI DAN IDENTIFIKASI BAKTERI PENDEGRADASI LIPID (LEMAK) PADA BEBERAPA TEMPAT PEMBUANGAN LIMBAH DAN ESTUARI DAM DENPASAR I. B. G. Gede Darmayasa
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Research on isolation and identification of lipid degrading bacteria in some sewage disposing sites and Denpasar Estuary Dam was carried out in April, 2006. This research aims to identify fat degrading bacteria on those areas.Isolation of bacteria was undertaken using thinning methods (platting methods) grown at sierra media. Colonies observed on the media after incubation on the temperature of 30-370C for 24 hours were identified based on macroscopic observations, biochemical tests, microscopic and cross tests to ensure that the bacteria identified was absolutely lipid degrading bacteria.The research result showed that number of lipid degrading bacteria isolates obtained from three sampling sites were 7 isolates. Four isolates were obtained from samples taken on BTDC Lagoons, the samples of which contains Bacillus sp. (A). Bacillus sp. (B), Klebsiella sp. and Staphylococcus sp., 2 isolates were gotten from samples found in estuary DAM with bacteria of Bacillus sp. (A) and Klebsiella sp. and 1 isolate was gotten from slaughterhouse waste disposal with Klebsiella sp. bacteria. Cross test results carried out on the seven isolates showed that two genera of bacteria only, namely: Bacillus sp. and Pseudomonas sp., which were viable after inoculation and 24 hours of incubation time.

UJI KANDUNGAN UNSUR RADIOAKTIF DAN BAKTERI PENCEMAR ESCHERICHIA COLI PADA LIMBAH INDUSTRI DI DAERAH HILIR SUNGAI BADUNG, DESA PEMOGAN Ni Made Indah Suandewi; I Gusti A.A. Ratnawati; Ida Bagus Gede Darmayasa
BULETIN FISIKA Vol 16 No 1 (2015): BULETIN FISIKA
Publisher : Departement of Physics Faculty of Mathematics and Natural Sciences, and Institute of Research and Community Services Udayana University, Kampus Bukit Jimbaran Badung Bali

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The radioactive element content and E.coli bacterial contaminants has been done testing in river water downstream of Badung River, Village Pemogan. The testing of elements content in the samples using AAS instrument (Atomic Absorption Sphectrometer) Analytical Laboratory Udayana University. The elements contained in the river water downstream of Badung River, Village Pemogan including Co, Cu, Pb, and Cd. The pollution in Badung river water to the elements thought due to the disposal of waste production by the textile dye industry was around the River Badung, Pemogan village. The test pollutant content of E.coli bacteria using the MPN (Most Probable Number). The results of the test bacteria by MPN method showed the presence of E.coli bacteria contaminants in Badung river water with amounts ranging from 0 to >11,000 samples MPN/100ml. According to PP 82 in 2001, the content of E.coli bacteria in the water of the river is allowed a maximum in the range of 2000 MPN/100ml water. This suggests that the content of E.coli bacteria in river water downstream of Badung River, Village Pemogan has exceeded a predetermined threshold.Keywords : Radioactive Elements, E.coli, AAS (Atomic Absorption Sphectrometer), MPN (Most Probable Number)

THE POTENTIAL OF CHITINASE ENZYMES PRODUCED BY CHITINOLYTIC BACTERIA ISOLATED FROM SHRIMP SHELLS AS BIOCONTROL CANDIDATES IN CONTROLLING PLANT PESTS Faldy Efriant Pangemanan; Ida Bagus Gede Darmayasa; Joko Wiryatno
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Plant pests is one of the problem in agriculture sector that can cause decreasing of harvest productivity. Plant pest invasion will be increased in rainy season, so it need to be controlled. Biological control by chitinolytic bacteria is one of many ways to avoid the using of chemical pesticide. The purpose of this research to find the type of chitinolytic bacteria and utilizing the enzyme chitinase as plant pest biocontrol. Test has been done with spray method of 5 mL with 10%, 20%. 30%, 40% and 50% concentration with 7 treatments include positive and negative controls and 3 repetition. Observation of larva death done for 24 hours after spray, where every 6 hours observations are made. Pseudomonas is a genus that has been identified as chitinolytic bacteria. Larva death is indicated by with percentage result insignificant death which is shown at the 10 – 30% concentration. The percentage result significant death which is shown at the 40 – 50% concentration. 40% and 50% is most effective concentration in killing fruit fly (Drosophila sp.).

Co-Authors A.A.S. A. Sukmaningsih Alayna Himawan Alfian Siswanto Anak Agung Gede Indraningrat Ariyanti Ni Kadek Ayu Amelia Bekti Revi Rahayu Cokorda Putra Deny Christine Sidabutar Dwi Dirgantini, Anak Agung Faldy Efriant Pangemanan Fania Agustini Flavia Fionessa Falenda Shanzet I Gede Sugita Artana I Gusti Agung Ayu Ratnawati I Gusti Ayu Laras Pratama Putri I Ketut Gede Rudita I KETUT MUKSIN I Ketut Sundra I Komang Adi Widyastama I Luh Putu Wiwin Astari I Made Dira Swantara I Made Sara Wijana I Made Sugiarta I Nyoman Winyana, I Nyoman I Putu Agus Hendra Wibawa I W BUDIARSA SUYASA I Wayan Kasa I Wayan Sudiarsa I Wayan Sukadana I.P.G. Ardhana Ida Ayu Gde Yadnyawati Ida Bagus Made Suaskara Ilham . Inna Narayani Joko Wiryatno Junita Hardini Kadek Feby Widyantari Kadek Sudarmi Komang Kartika Indi Swari Komang Krisna Lalu Muhammad Sakti Surya Jagat Lintang Pradhanawati Made Ria Defiani MADE SUSUN PARWANAYONI Martin Joni Meitini Proborini Wahyuni Nadia Khairunnisa Nasha Hadi Rahmadanty NI KADEK WIWIK SINTA DEWI Ni Komang Ayu Kumala Dewi Ni Komang Deny Julyeda Ni LUH ARPIWI Ni Luh Putu Eka Suartini Ni Made Indah Suandewi Ni Made Susun Parwanayoni Ni Putu Adriani Astiti Ni Putu Indiani Sahaning Karna Ni Putu Sinta Puspa Dewi Ni Putu Yuliatiningsih Ni Wayan Purni wirathi Ni Wayan Sudatri Nyoman Mega Antari Pande Gde Eka Mardiana Pande Gde Sasmita Julyantoro Pande Putu Christine Putri Purnami Pande Putu Purwaningsih Prayitna Dewi, Ida Ayu Putu Cindy Arista Putu Eka Sudaryatma Ratu Ayu Ningrat Retno Kawuri Rochmalia Juniarti Putri Sang Ayu Sri Satya Laksmi Utari SANG KETUT SUDIRGA Siti Didah Alawiah Siti Nurlatifah SRI LESTARI Suasthi, I Gusti Ayu Suta Arta Yan Ramona Yulita Salma Lani

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