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DAYA HAMBAT EKSTRAK KULIT DAUN LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 25923DAN Escherichia coli ATCC 25922 Ariyanti Ni Kadek; Darmayasa Ida Bagus Gede; Sudirga Sang Ketut
Jurnal Biologi Udayana Vol 16 No 1 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research aimed to determine the inhibited effect of Aloe (Aloe barbadensis Miller) rind extract to the growthof bacteria Staphylococcus aureus and Escherichia coli and the most effective concentration for the resistanceagainst both of bacteria tested. The results showed that the extracts of Aloe rind inhibited the growth of bacteriaStaphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. It was indicated by the formation ofinhibition zones with the largest diameter average occurred concentrations of 100% with the size 11,58 mm forthe Staphylococcus aureus and 6,81 mm in Escherichia coli. The highest concentration inhibited the growth ofStaphylococcus aureus ATCC 25923 was 100% and 75% for the Escherichia coli ATCC 25922.
UJI KEBERADAAN DAN KARAKTERISASI MIKROBA PELARUT FOSFAT PADA BERBAGAI MERK PUPUK ORGANIK Ni Luh Putu Eka Suartini; I.B.G. Darmayasa; I.P.G. Ardhana
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk menguji keberadaan, mengetahui total mikroba dan karakterisasi mikroba pelarut fosfat pada berbagai merek pupuk organik. Keberadaan mikroba pelarut fosfat dicirikan dengan terbentuknya zona bening pada media pikovskaya, dengan rata-rata total mikroba pelarut fosfat terbanyak terdapat pada Pupuk Botani (Pubotan) Tanah Subur dengan total mikroba 99,93 x 105 (CFU/g pupuk) sedangkan yang paling sedikit terdapat pada Pupuk Organik Kompos (Limbah Sapi) dengan total mikrobanya yaitu 33 x 105 (CFU/g pupuk). Isolat bakteri pelarut fosfat yang ditemukan yaitu 7 isolat dengan 3 isolat bakteri tergolong genus Klebsiella dan 4 isolat bakteri tergolong genus Enterobacter. Sedangkan koloni cendawan pelarut fosfat yang ditemukan yaitu 5 koloni cendawan pelarut fosfat dengan 3 koloni tergolong Aspergillus niger dan 2 koloni tergolong Aspergillus flavus.
Potensi Rhizopus spp. dalam mengendalikan pertumbuhan Aspergillus flavus FNCC6109 pada pakan konsentrat ayam broiler Lalu Muhammad Sakti Surya Jagat; Ida Bagus Gede Darmayasa; I Made Sara Wijana
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p06

Abstract

Aspergillus flavus contamination of agriculture in Indonesia can cause problems to animal health and productivity. Some factors can support the appearance of contamination in feed, especially temperature and humidity. The main objective of this research was to investigate potency of Rhizopus spp. on inhibit A. flavus FNCC6109 in broiler chicken concentrate feed. The experiments were conducted dual culture method and the inhibition test of the Rhizopus spp. filtrate culture was incubated for 3, 4 and 5 days on in vitro. The in vivo test was directly applied in broiler chicken concentrate feed which added Rhizopus spp. filtrate culture concentration at 10% (v/v), 20% (v/v), 30% (v/v), 40% (v/v), dan 50% (v/v). The results showed that the Rhizopus spp. filtrate culture significantly (P?0,05) to inhibit the growth of A. flavus FNCC6109 both in vitro and in vivo. The percentage inhibition of Rhizopus spp. filtrate culture incubated for 5 days showed 67,47±2,10% relatively better results than 3 and 4 days, and therefore was used in the in vivo. Application of 50% (v/v) Rhizopus spp. filtrate culture to the broiler chicken concentrate feed significantly reduced 82% population of A. flavus FNCC6109 after 15 days incubated relative to that of negative control (concentrate feed without addition Rhizopus spp. filtrate culture and A. flavus FNCC6109).
SKRENING BAKTERI TOLERAN PESTISIDA DENGAN BAHAN AKTIF KLORANTRANILIPROL ASAL TANAH PERTANIAN BATURITI TABANAN BALI NI KADEK WIWIK SINTA DEWI; IDA BAGUS GEDE DARMAYASA; I KETUT SUNDRA
Jurnal Biologi Udayana Vol 21 No 1 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.887 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i01.p01

Abstract

In Indonesia agriculture practice often used the large scale pesticide application such as insecticide, herbicide, and fungicide. The wide use of toxic pesticide has created numerous problem in increasing environtmental hazard to human and to other animals. Many of soil bacteria had important role to degrading chemical compounds into simpler compounds as a bioremediation agent. The aim of this study was to screen the Chlorantraniliprole tolerant bacteria using soil mineral medium with Prevathon pesticide addition, also teo identificate the species of bacteria. This research was conducted at Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Udayana University. The research was done in three analysis, (1) bacteria test on Prevathon pesticide addition to mineral medium treatment, (2) characterization of bacteria, (3) Identification of pesticide tolerant bacteria with BD BBL Crystal Enteric/Non FermenterID Kit. The results showed that mineral medium with the addition of pesticides Prevathon treatment able to provide a significant different effect on the enrichment stage 1, stage 2 and stage 3 (P<0.05), there was 5 isolates pesticide tolerant bacteria that isolated from Baturiti Tabanan cultivated soil that was BSP 1, BSP 2, BSP 3 known as gram negative bacteria, and BSP 4, BSP 5 known as Gram positive bacteria, pesticide tolerant bacteria identified as Serratia marcescens which is a Gram negative bacteria group and may cause pathogenic.
DAYA HAMBAT FRAKSINASI EKSTRAK SEMBUNG DELAN (SPHAERANTUS INDICUS L) TERHADAP BAKTERI ESCHERICIA COLI DAN STAPHYLOCOCCUS AUREUS IDA BAGUS GEDE DARMAYASA
Jurnal Biologi Udayana Vol 12 No 2 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

A research entitled "Inhibitid activity of plant fractination extract of Sembung Delan (Sphaeranthus indicus L.) to Eschericia coli and Staphylococus aureus bacterial was carried aut to find the active of plant extract that responsible for bactericidal agent. This research used absorbance disc method to determine inhibitid fractionation extratc of Sembung Delan to growth of Eschericia coli and Staphylococus aureus bacterial. Results of this study showed that the crude extract of Sembung Delan inhibited the growth of Eschericia coli and Staphylococus aureus on Muller- Hinton medium, indicated by the formation of inhibition zone around the disc loaded with plant extract at concentration of 10,000 ppm, 20,000 ppm, 30,000 ppm, 40,000 ppm, and 50,000 ppm, with diamtre of 10mm, llmm, 12mm, 13mm and 16 mm respectively to S. aureus, forE. coli crude extract at concentration of 10,000 ppm, 20,000 ppm, 30,000 ppm, 40,000 ppm, and 50,000 ppm using diametre each of 12 mm, 13 mm, 15 mm, 17 mm and 19 mm respectively . From 17 fractions obtained from fractionation, fraction No. Vm (eluted with ethyl acetate:hexan) with Rf value of 0,9 ; 0,8 ; 0,7 ; 0,68 ; and 0,62, fraction No. DC (eluted with etihil acetate : hexan) with Rf value of : 0,98, ; 0,94 ; and 0,82, fraction No. XV (eluted with ethyl acetate 100%) Rf value of : 0.03; 0.23; 0.35 and fraction No. XVI (eluted with ethyl acetate 100%) with Rf value of: 0.03; 0.14; 0.17; 0.21, effectivity inhibited the growth of test bacterial.
SEMBUNG DELAN (Sphaerantus indicus L.) TERHADAP Pseudomonas solanacearum L. PENYEBAB PENYAKIT LAYU PADA TANAMAN TOMAT Ida Bagus Gede Darmayasa
Bumi Lestari Journal of Environment Vol 6 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A research entitled “Inhibitid activity of plant fractination extract of Sembung Delan(Sphaeranthus indicus L.) to the causal agent of bacterial wilt on tomato” was carried out to find outthe active fraction of plant extract that responsible for bactericidal activity againt P. solanacearum L.,Results of this study showed that the crude extract of S. indicus inhibited the growth of P.solanacearum L. on PDA. It was indicated by the formation of inhibition zone around the disc loadedwith plant extract at concentration of 0 ppm, 10,000 ppm, 20,000 ppm, 30,000 ppm, 40,000 ppm, and50,000 ppm. From 17 fractions obtained from fractionation, fraction No. XV (eluted with ethylacetate 100%) contains three compunds with Rf value: 0.03; 0.23; 0.35 and fraction No. XVI (elutedwith ethyl acetate 100%) contains four compunds with Rf value: 0.03; 0.14; 0.17; 0.21, effectivityinhibited the growth of P. solanacearum L
ISOLASI DAN IDENTIFIKASI BAKTERI PENDEGRADASI LIPID (LEMAK) PADA BEBERAPA TEMPAT PEMBUANGAN LIMBAH DAN ESTUARI DAM DENPASAR I. B. G. Gede Darmayasa
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

Research on isolation and identification of lipid degrading bacteria in some sewage disposing sites and Denpasar Estuary Dam was carried out in April, 2006. This research aims to identify fat degrading bacteria on those areas.Isolation of bacteria was undertaken using thinning methods (platting methods) grown at sierra media. Colonies observed on the media after incubation on the temperature of 30-370C for 24 hours were identified based on macroscopic observations, biochemical tests, microscopic and cross tests to ensure that the bacteria identified was absolutely lipid degrading bacteria.The research result showed that number of lipid degrading bacteria isolates obtained from three sampling sites were 7 isolates. Four isolates were obtained from samples taken on BTDC Lagoons, the samples of which contains Bacillus sp. (A). Bacillus sp. (B), Klebsiella sp. and Staphylococcus sp., 2 isolates were gotten from samples found in estuary DAM with bacteria of Bacillus sp. (A) and Klebsiella sp. and 1 isolate was gotten from slaughterhouse waste disposal with Klebsiella sp. bacteria. Cross test results carried out on the seven isolates showed that two genera of bacteria only, namely: Bacillus sp. and Pseudomonas sp., which were viable after inoculation and 24 hours of incubation time.
KECAK RAMAYANA DAN BALLET RAMAYANA WAKIL UNHI DI PENTAS INTERNATIONAL “KUMBH MELLA” TRIVANI, ALLAHABAD, ULTRA PARADESH INDIA UTARA Ida Ayu Gde Yadnyawati; I Nyoman Winyana; I Wayan Sukadana; I Made Sugiarta; I Wayan Sudiarsa; I Made Sudarsana; Pande Gde Eka Mardiana; Ida Bagus Putu Darmayasa; I Ketut Gede Rudita; I Luh Putu Wiwin Astari; Ida Ayu Prayitna Dewi; I Gusti Ayu Suasthi; Cokorda Putra
JURNAL SEWAKA BHAKTI Vol 3 No 2 (2019): Sewaka Bhakti
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1646.429 KB) | DOI: 10.32795/jsb.v3i1.520

Abstract

Pengaruh dan penyebaran Ramayana sebagai sebuah konsep cerita yang bersumber dari sastra Ramayana tidak dipungkiri telah menyebar di Indonesia semenjak Hindu dikenalkan. Ada berbagai sumber yang dapat dijadikan bukti hidupnya cerita Ramayana di dalam kehidupan masyarakat Hindu khususnya. Di Bali sendiri cerita ramayana tidak saja menjadi pergulatan pengamat budaya khususnya sastra-sastra yang seringkali secara eksis digemakan lewat bentuk seni budaya. Kecak merupakan salah satu bentuk karya seni pertunjukan klasik yang mengambil inspirasi dari cerita Ramayana. Sangat beralasan ketertarikan karena menggugah pandangan masyarakat tentang nilai-nilai yang terkandung di dalamnya menjadikan bentuk bentuk garapan semakin menarik. Kecak yang seringkali diasosiasikan dengan kera tampaknya memberikan sentuhan harmoni musikal yang sangat tepat dengan latar budaya Ramayana. Ketokohan yang mengagumkan diperlihatkan oleh Hanoman atau kera berbulu putih yang senantiasa hadir demi menjaga dharma atau kebenaran dianggap menjadi inspirasi di dalam kehidupan masyarakat Hindu di Bali Inspirasi tersebut tampaknya menjadi alasan kuat bagi seorang peneliti India untuk memperlihatkan kepada dunia tentang pengaruh wiracerita yang dianggap berhasil menginspirasi masyarakat dunia.
TARI BARIS KEKUPU DALAM UPACARA MAMUKUR DI BANJAR LEBAH DESA ADAT SUMERTA KAJA DENPASAR I Made Sudarsana; Anak Agung Dwi Dirgantini; Ida Bagus Darmayasa
WIDYANATYA Vol 2 No 01 (2020): WIDYANATYA
Publisher : UNHI PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32795/widyanatya.v2i01.630

Abstract

Merefleksikan unsur-unsur kesenian, khususnya seni pertunjukan, dalam ritual keagamaan, seni tari wali atau tari sakral merupakan salah satu unsur yang menunjang rangkaian segala upacara keagamaan yang ada di Bali. Artikel ini membahas salah satu tari sakral atau wali bernama Tari Baris Kekupu yang berada di Banjar Lebah Desa Adat Sumerta Kaja, Denpasar. Berdasarkan hasil penelitian, tarian ini merupakan bagian integral dari kehidupan masyarakat setempat yang selalu terikat dengan peristiwa ritual atau upacara Mamukur. Bentuk Tari Baris Kekupu, ditarikan oleh penari anak-anak perempuan karena didalam Tari Baris Kekupu ini terdapat unsur-unsur Tari Legong, Idenya terinspirasi dari hiasan kupu-kupu pada damar kurung yang dipasang saat upacara Mamukur. Selain itu, secara fungsional tarian ini dapat dipahami memiliki fungsi religius dan fungsi pelestarian budaya.
Potensi Bakteri Sebagai Biodegradasi Lemak Dan Minyak Pada Lingkungan Yang Tercemar Limbah Domestik Retno Kawuri; Ida Bagus Gede Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p18

Abstract

The contamination of waste oil and grease to the aquatic environment from industrial food processing, restaurants and kitchens or by accidental oil spills is increasingly being found. Biodegradation using a consortium of bacteria can be an effective way to overcome this. The aim of this research was to isolate and identify bacterial strains from waste oil and grease that are capable of biodegradation and the potential of degrading bacteria to degrade oil and grease in vitro. Samples were taken from waste oils and fats. Enrichment techniques were carried out for all samples followed by isolation of bacterial strains to determine which strains could degrade oil and fat in vitro. Identification of bacteria using the identification kit BBL Crystal System. The total bacteria count was carried out using the dilution method. The results showed that there were 3 strains of oil and fat degrading bacteria, namely Bacillus licheneformis, Bacillus coagulant and Psedomonas diminuta. The consortium of the three types of bacteria was able to degrade waste oil and fat with a total of 624x105 CFU / ml to 816x105 CFU / ml bacteria with a control without the addition of a bacterial consortium which was 56x101 CFU / ml. The total bacteria in various treatment wastes with a consortium of bacteria based on molasses in the invitro test showed the ability to live and develop from a fairly high bacterial consortium, namely 292x104 CFU / ml to 904x104 CFU / ml. The bacteria found had the ability to degrade working oils and fats. synergistically. The benefit of this research is that bacteria that have been found can be used as a starter for processing waste fats and oils in environments contaminated with domestic waste fats and oils.
Co-Authors A.A.S. A. Sukmaningsih Alayna Himawan Alfian Siswanto Ariyanti Ni Kadek Ayu Amelia Bekti Revi Rahayu Cokorda Putra Deny Christine Sidabutar Dwi Dirgantini, Anak Agung Faldy Efriant Pangemanan Fania Agustini Flavia Fionessa Falenda Shanzet I Gede Sugita Artana I Gusti Agung Ayu Ratnawati I Gusti Ayu Laras Pratama Putri I Ketut Gede Rudita I KETUT MUKSIN I Ketut Sundra I Komang Adi Widyastama I Luh Putu Wiwin Astari I Made Dira Swantara I Made Sara Wijana I Made Sugiarta I Nyoman Winyana, I Nyoman I Putu Agus Hendra Wibawa I W BUDIARSA SUYASA I Wayan Kasa I Wayan Sudiarsa I Wayan Sukadana I.P.G. Ardhana Ida Ayu Gde Yadnyawati Ida Bagus Made Suaskara Ilham . Inna Narayani Joko Wiryatno Junita Hardini Kadek Feby Widyantari Kadek Sudarmi Komang Kartika Indi Swari Komang Krisna Lalu Muhammad Sakti Surya Jagat Lintang Pradhanawati Made Ria Defiani MADE SUSUN PARWANAYONI Made Yuni Pratiwi Martin Joni Meitini Proborini Wahyuni Nadia Khairunnisa Nasha Hadi Rahmadanty NI KADEK WIWIK SINTA DEWI Ni Komang Ayu Kumala Dewi Ni Komang Deny Julyeda Ni LUH ARPIWI Ni Luh Putu Eka Suartini Ni Made Indah Suandewi Ni Made Susun Parwanayoni Ni Putu Adriani Astiti Ni Putu Indiani Sahaning Karna Ni Putu Sinta Puspa Dewi Ni Putu Yuliatiningsih Ni Wayan Purni wirathi Ni Wayan Sudatri Nyoman Mega Antari Pande Gde Eka Mardiana Pande Gde Sasmita Julyantoro Pande Putu Purwaningsih Prayitna Dewi, Ida Ayu Putu Cindy Arista Putu Eka Sudaryatma Ratu Ayu Ningrat Retno Kawuri Rochmalia Juniarti Putri Sang Ayu Sri Satya Laksmi Utari Sang Ketut Sudirga SANG KETUT SUDIRGA Siti Didah Alawiah Siti Nurlatifah SRI LESTARI Suasthi, I Gusti Ayu Suta Arta Yan Ramona Yulita Salma Lani