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Journal : BIOVALENTIA: Biological Research Journal

DIFFERENT NITRATE AND AMMONIUM LEVELS MEDIA ON CHANGES OF NITROGEN ASSIMILATION ENZYMES IN RICE Handoyo, Tri; Ilman Widuri, Laily; Pudji Restanto, Didik
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.901 KB) | DOI: 10.24233/biov.7.1.2021.204

Abstract

Nitrogen (N) is an important nutrient for the growth and development of rice plants, required in large quantity and often limiting factor of rice yields. The research was to understand the different sources and levels of nitrogen in rice plant on the activity of N assimilation enzymes, including nitrate reductase (NR), glutamine synthase (GS) content, glutamate synthase (Gogat) content, content, ammonium (NH4+) and nitrate (NO3-) content on the leaves. Paddy (Ciherang variety) was grown in sand media containing Hoagland solution with different sources (ammonium and nitrate) and levels (0.4, 0.8, 1.6, 3.2, 6.4, and 12.8 mM) of nitrogen. Nitrogen assimilation was observed from leaves at one month of age. The NR activity increased on both Nitrogen sources, it was a higher activity in media contained nitrate. Also, the activity of GS showed higher in media contains nitrate, but its activity was decreased after application 1.6 mM of nitrate and 3.2 mM of ammonium. Western blot analysis of GS1 and GS2 showed that the band pattern of protein was similar to these enzyme activities. Nitrate content in leaves gradually increased in both sources of nitrogen and higher than 3.2 mM ammonium application caused an increase in ammonium content in leaves, but the nitrate content decreased. This research resulted that the available source of N for rice was in nitrate form, easily by the rice plants during the growth stage.
Co-Authors Agus Sasmito, Agus Ahdatu Uli Khikamil Maulidiya Ahmad Ilham Tanzil Ahmad Rivaldy, Dhea Andari, Syamsiyah Yuli Dwi Arif, Muzayin Arifin, Muhammad Reza Arum, Ayu Puspita Assery, Syeh Azmi Saleh Azmia, Azmia Mar'atul Latipah Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kriswanto, Budi Candra Prayoga, Mohammad Damayanti, Dwi Putri Oktavia Dwi Putri Oktavia Damayanti Dwika Nano Hariyanto Efsari, Aqilla Nur Fanata, Wahyu Indra Duwi Fariza Oktaviani Galih Kusuma, Raditya Hakim, Mohammad Sulton Hakim, Saila Najma Haq, Ahmad Thoriq Izzul HARDIAN SUSILO ADDY Hopipah, Opi Ihsan, Daimil Intan Kartika Setyawati Irma Novita Sari Irza Guari Syah Fitri Juhardi, Ujang Kacung Hariyono Kartikasari, Subekah Nawa Khamidah, Khusna Kiki Ulfaniah Kiki Ulfaniah Laily Ilman Widuri Laily Ilman Widuri, Laily Ilman Latif, Arkanudin Lenny Widjayanthi Luh Putu Suciati Magfiroh, Illia Seldon Mahros Darsin Marcus dos Santos Soares MISWAR - Mochammad Amrun Hidayat Mohammad Ali Mudhor Mohammad Ubaidillah Muchammad Devara , Yusuf Muhammad Hazmi Muhammad Imron Rosadi Muhammad Nur Thoriq Alfy Muhammad Syauqi Munandar, Denna Eriani Nasti, Elyanisa Maharani Nisrina, Sofia Bunga Nur Widodo Parawita Dewanti Popy Hartatie Hardjo Pratiwi, Helti Anggiana Prayoga, Mohammad Candra Pudji Restanto, Didik Puji Rahayu Putu Gita Maya Widyaswari Mahayasih Rahadiyan, Aristyo Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Rizka Amalia Rony, Zahara Tussoleha Rudi Joelijanto Saputra, Tio Eka Saputro Gumelar, Bachtiar Sholeh Avivi Sigit Soeparjono Slameto Slameto Slameto Slameto SRI AYU MADE Sri Hartatik Sucipto Sucipto Suryo Prakoso, Suryo Tri Agus Siswoyo Tri Wahyu Saputra Ubaidillah, Mohammad Umar Santoso Ummi Sholikhah Veronenci Yuliarbi Farlisa Wachju Subchan Wulanjari, Distiana Yani Corvianindya Rahayu Yugistia Bima, Rafi Yuli Witono Zahratus Sakdiyah Zahratus Sakdiyah Zaiyin Rizky Ageng Maulidia