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TRI HANDOYO
Fakultas Pertanian Universitas Jember
Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Transportation Other

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Journal : e-Journal Pustaka Kesehatan

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Uji Aktivitas Protein Larut Air Umbi Porang (Amorphophallus muelleri Blume) terhadap Escherichia coli dan Staphylococcus aureus Putu Gita Maya Widyaswari Mahayasih; Tri Handoyo; Mochammad Amrun Hidayat
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The objective of this study was to determine antibacterial activity of water soluble protein of Porang (Amorphalus muelleri Blume) tuber. The water soluble protein extract was tested against both Eschericia coli and Staphylococcus aureus using filter paper disk method. The inhibition zone of bacterial growth was calculated for both of bacterial cultures. It was found that water soluble protein exhibited antibacterial activity at linear range 0.3-1.9 μg and 0.1-1.9 μg for E. coli and S. aureus respectively, sugesting that S. aureuswas more susceptible than E. coli to this protein. From the electrophoretic profile using 12.5% sodium dodecyl sulfate (SDS) and comassie blue staining (CBS), two protein bands were observed with molecular weight 17 and 19 kDa respectively. The water soluble protein were further evaluated using periodic acid staining (PAS). Pink band with molecular weight 250 kDa and > 250 kDa was found in PAS profile, sugesting the availability of carbohydrate molecule in water soluble protein extract of porang tubers.
Co-Authors Agus Sasmito, Agus Ahdatu Uli Khikamil Maulidiya Ahmad Ilham Tanzil Ahmad Rivaldy, Dhea Andari, Syamsiyah Yuli Dwi Arif, Muzayin Arifin, Muhammad Reza Arum, Ayu Puspita Assery, Syeh Azmi Saleh Azmia, Azmia Mar'atul Latipah Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kriswanto, Budi Candra Prayoga, Mohammad Damayanti, Dwi Putri Oktavia Dwi Putri Oktavia Damayanti Dwika Nano Hariyanto Efsari, Aqilla Nur Fanata, Wahyu Indra Duwi Fariza Oktaviani Galih Kusuma, Raditya Hakim, Mohammad Sulton Hakim, Saila Najma Haq, Ahmad Thoriq Izzul HARDIAN SUSILO ADDY Hopipah, Opi Intan Kartika Setyawati Irma Novita Sari Irza Guari Syah Fitri Juhardi, Ujang Kacung Hariyono Kartikasari, Subekah Nawa Khamidah, Khusna Kiki Ulfaniah Kiki Ulfaniah Laily Ilman Widuri Laily Ilman Widuri, Laily Ilman Latif, Arkanudin Lenny Widjayanthi Luh Putu Suciati Magfiroh, Illia Seldon Mahros Darsin Marcus dos Santos Soares MISWAR - Mochammad Amrun Hidayat Mohammad Ali Mudhor Mohammad Ubaidillah Muchammad Devara , Yusuf Muhammad Hazmi Muhammad Imron Rosadi Muhammad Nur Thoriq Alfy Muhammad Syauqi Munandar, Denna Eriani Nasti, Elyanisa Maharani Nisrina, Sofia Bunga Nur Widodo Parawita Dewanti Popy Hartatie Hardjo Pratiwi, Helti Anggiana Prayoga, Mohammad Candra Pudji Restanto, Didik Puji Rahayu Putu Gita Maya Widyaswari Mahayasih Rahadiyan, Aristyo Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Rizka Amalia Rony, Zahara Tussoleha Rudi Joelijanto Saputra, Tio Eka Saputro Gumelar, Bachtiar Sholeh Avivi Sigit Soeparjono Slameto Slameto Slameto Slameto SRI AYU MADE Sri Hartatik Suryo Prakoso, Suryo Tri Agus Siswoyo Tri Wahyu Saputra Ubaidillah, Mohammad Umar Santoso Ummi Sholikhah Veronenci Yuliarbi Farlisa Wachju Subchan Wulanjari, Distiana Yani Corvianindya Rahayu Yugistia Bima, Rafi Yuli Witono Zahratus Sakdiyah Zahratus Sakdiyah Zaiyin Rizky Ageng Maulidia