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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Wahana Peternakan Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Ni Wayan Kurniani Karja
Departemen Klinik Reproduksi Dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln. Agatis, Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Journal : HAYATI Journal of Biosciences

 1 
Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro NI WAYAN KURNIANI KARJA; MOKHAMAD FAHRUDIN; KAZUHIRO KIKUCHI
HAYATI Journal of Biosciences Vol. 16 No. 1 (2009): March 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.946 KB) | DOI: 10.4308/hjb.16.1.25

Abstract

In porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture medium. In vitro matured and fertilized oocytes were cultured for the first 2 days in iodoacetate at concentrations of 0, 1, or 2.5 µM. The proportion of viable embryos on day 4 decreased when Ia was added at concentration of 2.5 µM (P < 0.05). The development of cleaved embryos to the blastocyst stage on day 6 was significantly reduced by 1 µM iodoacetate and none of cleaved embryos in Ia-2.5 group develop to the blasyocyst stage (P < 0.05). More than 60% of embryos in Ia-2.5 group were arrested at the two-four-cell stage, and then arrested at the morula stage. These results indicate that glucose plays important roles in supporting the development of early stage of porcine embryos and that the inhibition of glucose metabolism may disrupt the management of energy production, leaving the embryos incompetence to develop. Key words: iodoacetate, glucose, early stage embryos, in vitro culture
Cysteamine in Maturation Medium Enhances Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro Feni Dwi Kartika Gulo; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
HAYATI Journal of Biosciences Vol. 27 No. 4 (2020): October 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.4.290

Abstract

Low nuclear maturation and fertilization rate is one obstacle in the in vitro embryo production which decrease embryo yield. This problem is presumable related with high production of reactive oxygen species (ROS) during maturation process. Glutathione (GSH) as an antioxidant is well known to overcome effect of ROS production. GSH synthesis in the cytosol part of the oocyte cytoplasm is influenced by cysteine availability. It is therefore, this research was conducted to evaluate the ability of cysteamine to provide cysteine availability as GSH precursor on the nuclear maturation and fertilization rate of sheep oocytes. Results of this experiment revealed additional cysteamine at 150 µm and 200 µm could significantly improve nuclear maturation rate. On the other side, although additional of cysteamine at 50 µm could not improve nuclear maturation rate, however 50 µm cysteamine in the maturation medium could significantly improve the fertilization rate. Based on those experiment results, it seems that the additional cysteamine might be improve not only GSH availability but also the oocyte quality which characterized the ability of pronuclear formation. This finding strongly suggested that additional cysteamine in the maturation medium could improve nuclear maturation and fertilization rate of sheep oocytes.
Co-Authors . Hasbi Abdullah Baharun Achmad Setiyono Adi Winarto Aisyah Fidela Siregar Alvien Nur Aini Amrozi Ananda Ananda Andhani Widya Hartanti Andriani Andriani Andriani Andriani Anita Hafid Arie Febretrisiana Arie Febretrisiana, Arie Asep Kurnia Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Purwantara Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bayu Sulistyo Bq Hayyul Hidayati Cece Sumantri Damanik, Asman Ramadhan Dhia Mardhia Engcong Diana Andrianita Kusumaningrum Dwitya Citraesti Edelina Sinaga Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Elmanaviean, Muhammad Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gusdinar, Rizal Gusman, Khalis Talitha Gustina, Sri Hafizuddin Hafizuddin Hariono Hasbi Hasbi Ian Kurniawan Iman Supriatna Indriastuti, Rhesti Kaiin, Ekayanti Mulyawati Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Khye, Kim Chwin Kim Chwin Khye Kurnia, Asep Kusdiantoro Mohamad Latifah Humairoh Lisa Dwi Fannessia Lisa Praharani M Agus Setiadi M Imron M. Elmanaviean Magfira Magfira Magfira Maghfira Maghfira Makhroja, Lalu Faris Naufal Masir, Ummul Masturi Muhajir Memili, Erdogan Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muttaqinullah Rabusin Neta Fitria Yasa Nugraha, Arifin Budiman Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oloan Parlindungan Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Pattikawa, Vapriel Andhika Praharani, Lisa Puwantara, Bambang R. Iis Arifiantini Reski Adelia Rizal Gusdinar Rizky Amrullah Chaniago Setiadi , Mohamad Agus Silvia Anggraini Siska Adelya Ramadhani Sri Estuningsih Sri Gustari Srihadi Agungpriyono Surya Agus Prihatno Surya Agus Prihatno Syafri Nanda Syahruddin Said Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Tike Sartika Tike Sartika Titis Prastiwi Triyaningrum, Triyaningrum Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Veronika Gilang Pravitasari Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina Winny Plumeria Aqshani Witri, Brilla Widya Yesi Pratiwi Kusumawati Yuke Rizky Amelia Yulida Nofa Yuni Siswani Zultinur Muttaqin