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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Wahana Peternakan Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Ni Wayan Kurniani Karja

Departemen Klinik Reproduksi Dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln. Agatis, Dramaga, Bogor 16680

Author-ID : 295831

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Kualitas Semen Kambing Sapera yang Dibekukan dalam Pengencer Tris Kuning Telur dengan Imbuhan Pentoxifylline (QUALITY OF SAPERA BUCK SEMEN FROZEN IN TRIS EGG YOLK EXTENDER ADDED WITH PENTOXIFYLLINE) Bq Hayyul Hidayati; Raden Iis Arifiantini; Ni Wayan Kurniani Karja; Diana Andrianita Kusumaningrum
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study was to determine the effective dose of pentoxyfilline (PTX) to improve the quality of sapera buck frozen semen in Tris Egg Yolk (TEY) extender. Two sexually mature sapera bucks aged 1.5 years old was used as semen source. Semen collected with artificial vagina. Fresh semen were evaluated macro and microscopically. Only ejaculates showing > 70% sperm motility were selected. Semen were divided into four aliquots tubes containing TEY extender with 0 mM, 3.5 mM, 5 mM and 6.5 mM PTX each tube. After dilution, semen were packed into mini straw (0.25 ml) and equilibrated at 5 oC for 4 hours, then frozen above liquid nitrogen vapors for 10 minutes before plunged into liquid nitrogen (-196 oC). Results of this study showed that 6.5 mM PTX addition to the TEY extender improve post-thaw semen motility (P<0.05) but not viability and plasma membrane integrity. It concluded that 6.5 mM PTX addition to TEY extender improved post-thaw sperm motility of sapera buck.

Kafein dalam Medium Maturasi Meningkatkan Fertilisasi dan Menekan Frekuensi Polispermi Oosit Domba dengan Maturasi Diperpanjang (CAFFEINE SUPLEMENTATION IN MATURATION MEDIUM IMPROVE NORMAL FERTILIZATION AND REDUCED THE FREQUENCY OF POLYSPERMY IN SHEEP OOC Reski Adelia; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this study were to determine kinetic of nuclear maturation and the efficacy of caffeine suplementation in maturation medium on fertilization rate of sheep oocytes in vitro. In the first experiment, oocytes were matured for 16 (M-16), 20 (M-20), 24 (M-24), 28 (M-28) h to assessed the kinetic of oocytes nuclear maturation. In the second experiment, oocytes were matured for 24 h (M-24) or 28 h (M-28) without (M-24 or M-28 groups) or with caffeine suplementation at 4 h before the end of maturation period of oocytes matured for 24 h (M24-Kaf-4) and 28 h (M28-Kaf-4), or 8 h (M28-Kaf-8) before the end of maturation period of oocytes matured for 28 h. Result of the first experiment, 27.6% of oocytes were in metaphase II (MII) at 16 h. The percentage of MII oocytes significantly increased after 20 h (44.8%) to 24 h (88.9%) of maturation period (P<0.05), but the increasing was not found when the maturation period was prolonged until 28 h (89.3%) (P>0.05). However the number of oocytes with two pronucleus (2PN) was higher in group M-24 compared than that of M-28 group (P<0,05) and incidence of polyspermy increased in oocytes of M-28 group (P<0,05). No significant diferences was found in the total of oocytes fertilized among the group except of group M28-Kaf-8 (P>0,05). When caffeine was suplemented at 4 h before the end of maturation period a significantly reduced the incidence of polyspermy and increased the number of oocytes with 2PN in oocytes of M-28 group (P<0.05). In conclusion, the kinetic of nuclear maturation in sheep oocytes showed there was a variation in time required by oocytes to reach MII phase and caffeine improve normal fertilization and reduced the frequency of polyspermy on oocytes when the maturation period prolonged. ABSTRAK Penelitian ini bertujuan untuk mengetahui kinetika maturasi inti dan efektivitas suplementasi kafein pada medium maturasi terhadap tingkat fertilisasi oosit domba secara in vitro. Penelitian tahap I oosit dimaturasi selama 16 (M-16), 20 (M-20), 24 (M-24), dan 28 (M-28) jam untuk mengevaluasi kinetika maturasi inti oosit. Penelitian tahap II, oosit dimaturasi selama 24 jam (M-24) dan 28 jam (M-28) tanpa kafein (kelompok M-24 dan M-28) atau dengan penambahan kafein pada empat jam sebelum akhir periode maturasi pada oosit yang dimaturasi selama 24 jam (M24-Kaf-4) dan 28 jam (M28-Kaf-4), atau delapan jam (M28-Kaf-8) sebelum akhir periode maturasi pada oosit yang dimaturasi selama 28 jam. Hasil penelitian tahap I menunjukkan, 27,6% oosit berada pada tahap metafase II (MII) pada jam ke-16. Persentasi oosit MII meningkat secara signifikan setelah jam ke-20 (44,8%) hingga jam ke-24 (88,9%) periode maturasi (P<0,05) akan tetapi tidak ditemukan adanya peningkatan ketika periode maturasi diperpanjang hingga 28 jam (89,3%) (P<0,05). Namun demikian, jumlah oosit dengan dua pronukleus (2PN) lebih banyak pada kelompok M-24 dibandingkan dengan kelompok M-28 (P<0,05) dan kejadian polispermi meningkat pada oosit kelompok M-28 (P<0,05). Tidak ditemukan adanya perbedaan yang signifikan pada tingkat fertilisasi total antar perlakuan kecuali pada kelompok M28-Kaf-8 (P<0,05). Ketika kafein ditambahkan pada empat jam sebelum akhir periode maturasi secara signifikan dapat menurunkan kejadian polispermi dan meningkatkan jumlah oosit 2PN pada kelompok M-28 (P<0,05). Dapat disimpulkan bahwa kinetika maturasi inti oosit domba menunjukkan ketidakseragaman waktu yang dibutuhkan oleh oosit untuk mencapai tahap MII dan kafein dapat meningkatkan fertilisasi normal dan menurunkan frekuensi polispermi pada oosit ketika periode maturasi diperpanjang

Fetal Bovine Serum Meningkatkan Maturasi Inti Oosit Kelinci Setelah Dimaturasi Secara In Vitro Ni Wayan Kurniani Karja; Winny Plumeria Aqshani; Yesi Pratiwi Kusumawati; Veronika Gilang Pravitasari; Sri Gustari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study was conducted to examine the meiotic competence or nuclear maturation of rabbit oocytesmatured in vitro. Oocytes were recovered by mincing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) with compact cumulus cell mass and a dark, homogenousooplasm were cultured in maturation medium at 38°C in a humidified atmosphere of 5% CO2 in air, andthen stained for nuclear maturation. Three experiments were carried out. In Experiment 1, COCs werecultured in maturation medium for 18-20, 22-24, and 28-30 h. The proportion of oocytes at metaphase II(MII) was similar (P>0.05) at 18-20 (69.2%), 22-24 (66.4%), and 28-30 (71.1) h of culture. In Experiment 2,COCs were cultured in either maturation medium containing 0.04% bovine serum albumine (BSA) or 5%fetal bovine serum (FBS) for 24 h. The proprotion of oocytes that reached MII were higher (P<0.05) in FBSgroup (79.2%) than those of oocytes in BSA group (64%). In Experiment 3, based on the presence or absenceof follicles and corpora lutea, the ovarian pairs were classified into follicular or luteal stages. There was nodifference (P>0.05) among oocytes collected from ovaries in follicular (79.7%) and luteal stages (78.7%) inthe ability to achieve nuclear maturation. These results indicated that nuclear maturation of rabbitoocytes in vitro was completed at 18-20 h of maturation culture and was not affected by ovary’s reproductivestage. The presence of FBS in the maturation medium enhanced the ability of rabbit oocytes to achievenuclear maturation.

Pelacakan Kerusakan Akrosom Spermatozoa Domba Selama Proses Pembekuan dengan Teknik Histokimia Lektin (DETECTION OF ACROSOMAL DAMAGE OF RAM SPERMATOZOA DURING FREEZING PROCESS USING LECTIN HISTHOCHEMICAL TECHNIQUE) Lisa Dwi Fannessia; Ni Wayan Kurniani Karja; I Ketut Mudite Adnyane; Mohamad Agus Setiadi
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Freezing process in ram spermatozoa caused damage of the plasma membrane and acrosome thatlead to the decrease of spermatozoa fertility. Research was conducted to evaluate acrosomal damageduring freezing process using lectin histochemical technique. Semen was collected twice a week usingartificial vagina from 1-2 years old Garut ram. Immediately after collection, characteristic of semenquality was evaluated then diluted with Niwa and Sasaki Freezing (NSF) medium. Semen was loadedinto 0.25 mL mini straws and equilibrated at 4oC for two hours. Straws were then frozen and stored inliquid nitrogen. Evaluation of sperm characteristic (motility, viability and plasma membrane integrity)and acrosomal integrity were done during the freezing process. Detection of acrosomal integrity was observedusing Fluorescens isothiocyanate (FITC) and Avidin-Biotin-Complex (ABC) staining methods. Data ofcharacteristic spermatozoa and acrosomal integrity were analyzed using ANOVA. Result of the experimentsshowed that the percentage of motility, viability and plasma membrane integrity of spermatozoa before freezing (83 ± 2.7%; 88.8 ± 2.6%; 88.2 ± 3.7%) were significantly decreased (P<0.05) after equilibration (71± 4.2%; 84.2 ± 5.0%; 76.2 ± 1.3%) and after thawing (40 ± 3.5%; 61.08 ± 3.3%; 51.2 ± 10.4%). The percentageof intact acrosomal spermatozoa using FITC and ABC methods during freezing process were 93.63 ±2.73%; 88.04 ± 3.2% and 81.73 ± 4.77% VS 94.54 ± 0.26%; 88.17 ± 0.38% and 79.38 ± 2.06%, respectively.In conclusion, the characteristic of spermatozoa were significantly decrease (P<0.05) during freezing process.Furthermore, the integrity of acrosome spermatozoa can be well analyzed during freezing process by usinglectin histochemical staining methods.

Tingkat Proliferasi Primordial Germ Cells secara In Vitro dalam Medium Kultur dengan Penambahan Leukemia Inhibitory Factor (IN VITRO PROLIFERATION RATE OF MICE PRIMORDIAL GERM CELLS IN THE CULTURE MEDIUM WITH ADDITION OF LEUKEMIA INHIBITORY FACTOR) Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Primordial germ cells (PGCs) are precursors for gamete cells. The totipotency of PGCs allows them to be used as a model for studying cancer and infertility. The study aimed to examine the characteristics of the mice fetus as a source of PGCs, proliferation rate of PGC and the role of LIF in vitro culture of PGCs. This study used genital ridges from 26 fetuses at 13.5 days post-coital (dpc) to isolate the PGCs. Genital ridges dissociation using 0.1% of trypsin and in vitro culture was carried out using the Dulbecco Modified Eagle Medium (DMEM) and incubated at 37 0C and 5% CO2 atmosphere. The fetus was measured and weighed to determine the normal development of the fetus and continued with the identification of the genital ridges after laparotomy performed under a stereomicroscope. Proliferation rate was measured by calculating Population Doubling Time (PDT), and cell viability was observed after in vitro culture for six days. The effect of adding 1000 IU/ml of leukemia inhibitory factor (LIF) was evaluated from two types of treatment in the medium, 1) DMEM added with 15% of fetal calf serum (FCS) (DMEM + S15%) and 2), DMEM was supplemented with 15% of FCS and 1000 IU/ml LIF (DMEM + S15% + LIF1000 IU/ml). Immunohistochemistry staining was carried out on the third-, sixth- and ninth-day of culture to detect the expression of Oct-4 in the PGCs, then cells were counted. The results showed that the fetus as a source of PGCs had normal development. The fetal sizes were 11 mm, and male and female genital ridges could be distinguished by morphology at the age of 13.5 dpc. The proliferation of PGCs was relatively slow with a 1.3 day PDT value with the viability of around 85%. Culture of PGCs with DMEM + S15% treatment showed the percentage of PGCs that expressing Oct-4 decreased from the third day of culture to the ninth day of culture. The culture of PGCs in DMEM + S15% + LIF 1000 IU / ml treatment showed that the percentage of PGCs that expressed Oct 4 increased on the sixth day of culture and decreased on the ninth day of culture. It can be concluded that the addition of LIF can maintain the number of PGCs until the sixth day of culture. LIF is thought to play a role in the regulation of proliferation of PGCs through receptors of LIF (RLIF) and glicoprotein (gp) 130 receptors.

Kualitas dan Tingkat Maturasi Oosit Kucing Domestik dari Ovarium yang Disimpan dalam Waktu dan Media yang Berbeda Ni Wayan Helpina Widyasanti; Ni Wayan Kurniani Karja; Ekayanti Mulyawati Kaiin; Mohamad Agus Setiadi
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mengevaluasi kualitas dan tingkat maturasi oosit kucing domestik yang disimpan dalam waktu dan media yang berbeda. Ovarium yang diperoleh setelah ovaryohisterectomy disimpan dalam tabung steril dan cara penyimpanannya dibagi menjadi tiga perlakuan , yaitu: 1) tanpa media, 2) berisi NaCl 0,9% atau 3) berisi PBS. Ovarium tersebut kemudian dibawa ke laboratorium dengan termos yang berisi NaCl 0,9% dengan suhu 35-37°C atau dengan cooler box suhu 4°C. Sampel ovarium suhu 4°C kemudian disimpan dalam refrigerator dengan suhu 4°C selama 24 dan 48 jam. Oosit dari ovarium yang dibawa dengan suhu suhu 35-37°C dikoleksi dalam waktu di bawah enam jam setelah sampai di laboratorium. Pada akhir penyimpanan, oosit dikoleksi dan dievaluasi kualitasnya. Selanjutnya, oosit dimaturasi dan dievaluasi tingkat maturasinya. Hasil dari penelitian ini menunjukkan bahwa berdasarkan morfologinya kualitas oosit kucing tidak dipengaruhi oleh waktu dan jenis media selama penyimpanan (P>0,05). Tingkat maturasi oosit untuk mencapai tahap MII mulai menurun (P<0,05) pada ovarium yang disimpan tanpa media maupun dengan PBS pada 24 jam periode penyimpanan, sedangkan oosit yang berasal dari ovarium yang disimpan dengan NaCl 0,9% mulai menurun (P<0,05) pada 48 jam periode penyimpanan. Simpulan pada penelitian ini adalah penyimpanan ovarium dengan atau tanpa media selama 48 jam tidak memengaruhi morfologi oosit kucing namun memengaruhi tingkat maturasi oosit kucing.

Status DNA dan Karakteristik Spermatozoa Kauda Epididimis Domba Pascapenyimpanan pada Suhu 4oC (DNA STATUS AND CHARACTERISTIC OF SPERM CAUDA EPIDIDYMAL RAM AFTER STORAGE AT 4oC) Ummul Masir; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to evaluate the deoxyribonucleic acid (DNA) status and characteristics of ram spermatozoa, maintained within epididymides stored at 4ºC. A total of 12 pairs of ram cauda epididymis were kept by means that one of the pair in a tube contain media (isotonic NaCl solution) and the other pair into a clean ziplock without medium then stored at 4ºC for three days. Following this, the motility and viability and DNA status of spermatozoa was observed using halomax sperm method, prior and post freezing process. The results showed a decreased in the percentage of motility and viability of spermatozoa from cauda epididymis which were kept in and without media, before or after cryopreservation (P <0.05). Based on the storage method, a significant difference in motility percentage occurs at day two and day three of the storage. Storage using media had a lower percentage of motility (23%; 10%) than without using media (50%; 37%) (P <0.05). The DNA status following storage at 4ºC for three days, descriptively showed impaired of spermatozoa DNA less than 1%. The percentage of impairment increased following the cryopreservation of spermatozoa due to the freezing process. This condition was especially observed on the third day of storage of cauda epididymis which were kept in media (10.16%). The characteristics of spermatozoa from cauda epididymis which were kept in the media were lower compared to without medium. The DNA status of cauda epididymis spermatozoa is not affected by the storage method. ABSTRAK Penelitian ini bertujuan untuk mengevaluasi status DNA dan karakterisik spermatozoa asal kauda epididimis domba yang disimpan pada suhu 4ºC. Sebanyak 12 pasang kauda epididimis domba disimpan dengan cara salah satu dari setiap pasang kauda epididimis dimasukan ke dalam tabung berisi media (NaCl fisiologis) dan pasangan yang lain ke dalam ziplock bersih (tanpa media). Penyimpanan dilakukan selama tiga hari pada suhu 4ºC kemudian dilihat motilitas dan viabilitasnya serta status DNA spermatozoa dengan metode sperm sus halomax, sebelum dan setelah pembekuan. Hasil penelitian menunjukkan bahwa persentase motilitas dan viabilitas spermatozoa asal kauda epididimis baik yang disimpan dengan atau tanpa media mengalami penurunan, sebelum atau setelah kriopreservasi (P<0,05). Berdasarkan metode penyimpanan, perbedaan persentase motilitas yang nyata terjadi pada hari ke dua dan ke tiga penyimpanan. Penyimpanan menggunakan media memiliki persentase motilitas yang lebih rendah (23%; 10%) dibandingkan dengan tanpa menggunakan media (50%; 37%) (P<0,05). Status DNA spermatozoa setelah dilakukan penyimpanan pada suhu 4ºC selama tiga hari, secara deskriptif memerlihatkan kerusakan DNA spermatozoa kurang dari 1%. Persentase kerusakan kemudian meningkat setelah kriopreservasi spermatozoa akibat dari pembekuan, terutama pada hari ke tiga penyimpanan kauda epididimis dengan media (10,16%). Karakteristik spermatozoa dari kauda epididimis yang disimpan dengan media lebih rendah dibandingkan tanpa media. Status DNA spermatozoa kauda epididimis tidak dipengaruhi oleh metode penyimpanan.

Karakteristik Frozen-thawed Spermatozoa Domba Garut yang Dikriopreservasi dalam Pengencer yang Mendapat Imbuhan Orvus ES Paste (THE CHARACTERISTIC OF FROZEN-THAWED GARUT RAM SPERMATOZOA CRYOPRESERVED IN EXTENDER SUPPLEMENTED WITH ORVUS ES PASTE) Andhani Widya Hartanti; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the present study was to investigate the characteristics of frozen-thawed Garut ramsemen when cryopreserved in extender supplemented with Orvus Es Paste (OEP) as surfactant. Ramsemen were collected and evaluated, then cryopreserved in extender supplemented with OEP at differentconcentrations: OEP-0 (without OEP); OEP-0.25%; OEP-0.50%; OEP-0.75%; and OEP-1.00%. Thecharacteristics observed were motility, viability, and Intact Plasma Membrane (IPM). The results showedthat semen which were cryopreserved and supplemented with OEP had a higher motility, viability, andIPM, respectively compared to those without OEP. No significant differences in the characteristics wereobserved within the different OEP concentrations. It can be concluded that supplementation of OEP in theextender could protect the quality of cryopreserved ram spermatozoa.

Kompetensi Maturasi dan Fertilisasi Oosit Domba Prapubertas Secara In Vitro (DEVELOPMENTAL COMPETENCE OF MATURATION AND FERTILIZATION PREPUBERTAL SHEEP OOCYTES IN VITRO) Anita Hafid; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this study was to evaluate the maturation and fertilization ability of oocytes frompre-pubertal sheep ovary in vitro. Prepubertal ovary were collected based on the absence of corpus luteum or absence of corpus albicans in both of the ovaries. Oocytes were collected by slicing methode. Only the oocytes which categories of homogeneous cytoplasm and compact cumulus cells were used and it was matured for 24 hours in CO2 incubator with temperature 39oC. Oocytes fertilized in vitro used post thawed spermatozoa with concentration 5x106 and incubated for 12-14 hours. Oocytes were evaluated on number of oocytes reached MII and number of PN formation. Result of the experiment revealed that there was no significant difference in the percentage of MII oocytes after in vitro maturation (89% vs 90,7%, P>0,05) between prepubertal sheep and pubertal sheep. Meanwhile, the fertilization rate was significantly lower (P>0,05) in prepubertal sheep oocytes compared to pubertal sheep oocytes (60% vs 77,7%). The incidence of polispermic fertilization was higher in prepubertal sheep oocytes than pubertal sheep oocytes (21,8% vs7,4%, P>0,05). In conclusion, prepubertal sheep oocytes and pubertal sheep oocytes have similar in vitromaturation ability, even though the ability to be fertilized of the prepubertal sheep oocytes is lower than the pubertal sheep. ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan maturasi dan fertilisasi oosit dari ovarium domba prapubertas secara in vitro. Ovarium domba prapubertas dikoleksi berdasarkan ketidakhadiran korpus luteum atau ketidakhadiran korpus albicans pada kedua ovarium. Oosit dikoleksi dengan metode slicing. Hanya oosit dengan sitoplasma yang homogen dan sel kumulus yang kompak yang dipakai dan dimaturasi selama 24 jam dalam inkubator CO2 dengan suhu 39oC. Oosit difertilisasi secara in vitro menggunakan semen beku dengan konsentrasi 5x106 spermatozoa/mL dan diinkubasi selama 12-14 jam. Pengamatan dilakukan terhadap kemampuan oosit mencapai tahap metafase II (MII) dan pembentukan pronukleus (PN). Hasil penelitian menunjukkan status inti oosit pada tahap MII tidak berbeda antara oosit domba prapubertas dan oosit domba pubertas (89% vs 90,7%, P>0,05) setelah dimaturasi secara in vitro. Sementara tingkat fertilisasi oosit domba prapubertas lebih rendah (P>0,05) dibandingkan dengan oosit domba pubertas (60,0% vs 77,7%). Kejadian polispermi pada oosit domba prapubertas cenderung lebih tinggi dibandingkan dengan oosit domba pubertas (21,8% vs 7,4%, P>0,05). Dapat disimpulkan bahwa oosit domba prapubertas memiliki kemampuan maturasi yang sama dengan oosit domba pubertas namun memiliki kemampuan fertilisasi yang lebih rendah.

POLA GERAKAN SPERMA SAPI SETELAH DIINKUBASI SECARA IN VITRO DALAM MEDIA FERTILISASI DENGAN IMBUHAN HEPARIN DAN/ATAU KAFEIN Achmad Setiyono; Ni Wayan Kurniani Karja; Mohammad Agus Setiadi; Ekayanti Mulyawati Kaiin
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Penelitian ini bertujuan untuk mengevaluasi penambahan heparin dan kafein secara tunggal maupun kombinasi terhadap status pola gerakan sperma selama proses kapasitasi in vitro. Semen beku yang sudah di thawing diinkubasi di dalam media fertilisasi saja atau ditambahkan dengan kafein 2 mM, heparin 10 µg/mL, dan kombinasi kafein 2 mM dan heparin 10 µg/mL selama 60 menit. Total motilitas, motilitas progresif dan pola gerakan sperma (VCL; LIN dan ALH) dievaluasi menggunakan CASA. Evaluasi dilakukan sebelum inkubasi atau 0, 15, 30 dan 60 menit setelah inkubasi di suhu 38.5 oC dan 5% CO2. Hasil penelitian menunjukkan VCL sperma tidak berbeda pada setiap kelompok dengan periode waktu yang berbeda (P>0.05), kecuali pada MF-Caf-2. persentase LIN pada MF-Caf-5 ditemukan lebih rendah dibandingkan kelompok lainnya sampai pada menit ke-30 (P<0.05), kemudian pada menit ke-30 nilainya sama dengan MF-Caf-2 (P>0.05). Segera sebelum inkubasi, ALH sperma pada MF-Caf-5 lebih tinggi daripada MF (P<0.05), tetapi tidak berbeda dengan MF-Caf-2 dan MF-Hep-10 (P>0.05). Sperma mengalami hiperaktif motilitas terjadi selama periode inkubasi dengan menambahkan kafein. Total motilitas sperma mulai menurun pada menit ke-30 pada MF dan dan MF-Hep-10 dan pada menit ke-60 pada MF-Caf-2 (P<0.05). Total motilitas sperma antar kelompok perlakuan pada periode waktu yang sama juga ditemukan tidak berbeda (P>0.05), tetapi motilitas progresif pada menit ke-60 lebih tinggi pada MF-Caf-5-Hep-10 jika dibandingkan dengan MF-Caf-2-Hep-10 (P<0.05). Dapat disimpulkan bahwa penambahan kafein secara tunggal atau dikombinasikan dengan heparin dapat menginduksi terjadinya hiperaktivasi dengan tidak terjadinya penurunan motilitas secara signifikan selama 60 menit selama periode inkubasi.

Co-Authors . Hasbi Abdullah Baharun Achmad Setiyono Adi Winarto Aisyah Fidela Siregar Alvien Nur Aini Amrozi Ananda Ananda Andhani Widya Hartanti Andriani Andriani Andriani Andriani Anita Hafid Arie Febretrisiana Arie Febretrisiana, Arie Asep Kurnia Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Purwantara Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bayu Sulistyo Bq Hayyul Hidayati Cece Sumantri Damanik, Asman Ramadhan Dhia Mardhia Engcong Diana Andrianita Kusumaningrum Dwitya Citraesti Edelina Sinaga Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Elmanaviean, Muhammad Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gusdinar, Rizal Gusman, Khalis Talitha Gustina, Sri Hafizuddin Hafizuddin Hariono Hasbi Hasbi Ian Kurniawan Iman Supriatna Indriastuti, Rhesti Kaiin, Ekayanti Mulyawati Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Khye, Kim Chwin Kim Chwin Khye Kurnia, Asep Kusdiantoro Mohamad Latifah Humairoh Lisa Dwi Fannessia Lisa Praharani M Agus Setiadi M Imron M. Elmanaviean Magfira Magfira Magfira Maghfira Maghfira Makhroja, Lalu Faris Naufal Masir, Ummul Masturi Muhajir Memili, Erdogan Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muttaqinullah Rabusin Neta Fitria Yasa Nugraha, Arifin Budiman Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oloan Parlindungan Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Pattikawa, Vapriel Andhika Praharani, Lisa Puwantara, Bambang R. Iis Arifiantini Reski Adelia Rizal Gusdinar Rizky Amrullah Chaniago Setiadi , Mohamad Agus Silvia Anggraini Siska Adelya Ramadhani Sri Estuningsih Sri Gustari Srihadi Agungpriyono Surya Agus Prihatno Surya Agus Prihatno Syafri Nanda Syahruddin Said Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Tike Sartika Tike Sartika Titis Prastiwi Triyaningrum, Triyaningrum Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Veronika Gilang Pravitasari Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina Winny Plumeria Aqshani Witri, Brilla Widya Yesi Pratiwi Kusumawati Yuke Rizky Amelia Yulida Nofa Yuni Siswani Zultinur Muttaqin

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Journal : Jurnal Veteriner