Garuda - Garba Rujukan Digital

Efektifitas Antibakteri Ekstrak Etanol Daun dan Biji Asam Jawa (Tamarindus indica) dari Pulau Timor NTT dalam Menghambat Bakteri Salmonella typhi Felisitas Meli Podhi; Retno Kawuri; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p12

Pisang merupakan pangan lokal yang mengandung karbohidrat, lemak, dan protein yang tinggi dalam mencukupi kebutuhan nutrisi berpotensi memperbaiki metabolism tubuh. Penelitian ini bertujuan untuk mengidentifikasi gambaran histologi hati dan penambahan berat badan pada tikus model malnutrisi setelah pemberian tepung pisang. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 ulangan, menggunakan tikus jantan (strain wistar), umur 7 minggu, rerata berat badan 120 g sejumlah 24 ekor. Kelompok perlakuan diantaranya; K- (pakan standar), K+ (pakan nasi aking), M+P0 (pakan standar), M+P1 (pakan 100% tepung pisang), M+P2 (pakan 75% tepung pisang +25% pakan standar), M+P3 (pakan 50% tepung pisang + 50% standar). Kelompok perlakuan K+ sampai perlakuan M+P3 dibuat malnutrisi dengan diberi nasi aking. Kelompok K- diberi pakan standar selama 11 minggu. Pengukuran berat badan dilakukan setiap minggu untuk masing- masing perlakuan. Setelah 11 minggu, tikus dikorbankan. Organ hati dikoleksi kemudian dibuat mikroanatomi dengan pewarnaan Hematoxylin-Eosin. Parameter kerusakan hati dideteksi melalui jumlah sel nekrosis, degenerasi hidropik, dan apoptosis pada 100 sel yang diamati. Hasil penelitian pemberian tepung pisang pada perlakuan M+P3 dapat menurunkan jumlah kerusakan sel nekrosis (p<0,05). Kombinasi pakan 50% tepung pisang dan 50% pakan standar dapat mempercepat regenerasi, menurunkan jumlah kerusakan sel, dan menghasilkan peningkatan berat badan secara signifikan (p<0,05).

Uji Pemeriksaan Cemaran Total Bakteri, Coliform, Escherichia coli, dan Salmonella sp. pada Ikan Mujair (Oreochromis mossambicus) Hasil Budidaya Keramba Jaring Apung (KJA) di Kawasan Danau Batur, Kintamani, Bangli, Bali NI Putu Diah Septiani Putri; Retno Kawuri
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p7

Fish is a food that rots easily due to contamination with microorganisms. When consumed, food that has been contaminated with pathogenic bacteria can endanger human health. Tilapia fish (Oreochromis mossambicus) is vertebrate animal that is often cultivated, especially in the Kintamani area. This research was conducted to determine total bacterial contamination, Coliform, E.coli, and Salmonella sp. on tilapia fish cultivated in floating net cages in the Lake Batur area, Kintamani, providing information on the safety and suitability of consuming tilapia fish by food safety standards, as well as providing an overview of differences in bacterial contamination in each location village. The samples were tested using the pour plate and streak plate plating method. Coliform and E. coli bacterial contamination tests were carried out using the Most Probable Number (MPN) method, examining Salmonella sp. carried out on selective Salmonella Shigella Agar (SSA) media. The ALT test results obtained the highest average in tilapia fish samples from Kedisan village, namely 5.9 x 106 CFU/g, the highest average total Coliform was in fish samples from Abang and Batur Tengah villages with values > 1.1 x 104 MPN /g, the highest average E. coli contamination was shown by tilapia fish samples from Abang village, namely >4.64 x 103 MPN/g, and one sample from Batur Tengah village showed positive for Salmonella sp bacteria. Based on these results, all tilapia fish samples exceeded the threshold for ALT, Coliform and E. coli contamination and one sample exceeded the normal limit for Salmonella sp contamination. on food based on BPOM and SNI standards.

Deteksi Cemaran Total Bakteri, Coliform, Escherichia coli, dan Salmonella sp. pada Daging Ayam Broiler (Gallus domesticus) yang Dijual di Beberapa Pasar Daerah Kabupaten Badung, Bali Kadek Aryndha Sukma Prabaswari; Retno Kawuri; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p4

Chicken meat is a source of animal protein which is easily damaged due to its high water content. Chicken meat that is bought and sold could potentially become a place for the growth of bacteria that cause foodborne diseases.This study aims to determine total bacterial contamination, Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat in Badung Regency and determine the suitability of broiler chicken meat samples following food safety standards. Samples were taken using a purposive sampling method in six sub-districts in Badung Regency, namely Petang, Abiansemal, Mengwi, North Kuta, Kuta and South Kuta sub-districts at traditional markets that sell broiler chicken meat. The samples were tested using the pour plate and streak platting method. Parameters tested include Total Plate Number (ALT), Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat samples. Coliform and Escherichia coli bacteria were detected using Petrifilm ready-to-use media, while Salmonella sp. tested on selective Salmonella-Shigella Agar (SSA) media and catalase test and Gram staining. The research result showed that the contamination rates in broiler chicken breast meat were: Total Plate Number (ALT) 105 CFU/g to 107 CFU/g; Coliform and Escherichia coli 101 CFU/g to 104 CFU/g; Salmonella sp. positive in all samples, so that the samples tested did not meet the safety quality requirements for chicken meat according to SNI 3924: 2009.

Efektifitas Antibakteri Ekstrak Etanol Daun dan Biji Asam Jawa (Tamarindus indica) dari Pulau Timor NTT dalam Menghambat Bakteri Salmonella typhi Felisitas Meli Podhi; Retno Kawuri; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p12

Pisang merupakan pangan lokal yang mengandung karbohidrat, lemak, dan protein yang tinggi dalam mencukupi kebutuhan nutrisi berpotensi memperbaiki metabolism tubuh. Penelitian ini bertujuan untuk mengidentifikasi gambaran histologi hati dan penambahan berat badan pada tikus model malnutrisi setelah pemberian tepung pisang. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 ulangan, menggunakan tikus jantan (strain wistar), umur 7 minggu, rerata berat badan 120 g sejumlah 24 ekor. Kelompok perlakuan diantaranya; K- (pakan standar), K+ (pakan nasi aking), M+P0 (pakan standar), M+P1 (pakan 100% tepung pisang), M+P2 (pakan 75% tepung pisang +25% pakan standar), M+P3 (pakan 50% tepung pisang + 50% standar). Kelompok perlakuan K+ sampai perlakuan M+P3 dibuat malnutrisi dengan diberi nasi aking. Kelompok K- diberi pakan standar selama 11 minggu. Pengukuran berat badan dilakukan setiap minggu untuk masing- masing perlakuan. Setelah 11 minggu, tikus dikorbankan. Organ hati dikoleksi kemudian dibuat mikroanatomi dengan pewarnaan Hematoxylin-Eosin. Parameter kerusakan hati dideteksi melalui jumlah sel nekrosis, degenerasi hidropik, dan apoptosis pada 100 sel yang diamati. Hasil penelitian pemberian tepung pisang pada perlakuan M+P3 dapat menurunkan jumlah kerusakan sel nekrosis (p<0,05). Kombinasi pakan 50% tepung pisang dan 50% pakan standar dapat mempercepat regenerasi, menurunkan jumlah kerusakan sel, dan menghasilkan peningkatan berat badan secara signifikan (p<0,05).

Uji Pemeriksaan Cemaran Total Bakteri, Coliform, Escherichia coli, dan Salmonella sp. pada Ikan Mujair (Oreochromis mossambicus) Hasil Budidaya Keramba Jaring Apung (KJA) di Kawasan Danau Batur, Kintamani, Bangli, Bali NI Putu Diah Septiani Putri; Retno Kawuri
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p7

Fish is a food that rots easily due to contamination with microorganisms. When consumed, food that has been contaminated with pathogenic bacteria can endanger human health. Tilapia fish (Oreochromis mossambicus) is vertebrate animal that is often cultivated, especially in the Kintamani area. This research was conducted to determine total bacterial contamination, Coliform, E.coli, and Salmonella sp. on tilapia fish cultivated in floating net cages in the Lake Batur area, Kintamani, providing information on the safety and suitability of consuming tilapia fish by food safety standards, as well as providing an overview of differences in bacterial contamination in each location village. The samples were tested using the pour plate and streak plate plating method. Coliform and E. coli bacterial contamination tests were carried out using the Most Probable Number (MPN) method, examining Salmonella sp. carried out on selective Salmonella Shigella Agar (SSA) media. The ALT test results obtained the highest average in tilapia fish samples from Kedisan village, namely 5.9 x 106 CFU/g, the highest average total Coliform was in fish samples from Abang and Batur Tengah villages with values > 1.1 x 104 MPN /g, the highest average E. coli contamination was shown by tilapia fish samples from Abang village, namely >4.64 x 103 MPN/g, and one sample from Batur Tengah village showed positive for Salmonella sp bacteria. Based on these results, all tilapia fish samples exceeded the threshold for ALT, Coliform and E. coli contamination and one sample exceeded the normal limit for Salmonella sp contamination. on food based on BPOM and SNI standards.

Deteksi Cemaran Total Bakteri, Coliform, Escherichia coli, dan Salmonella sp. pada Daging Ayam Broiler (Gallus domesticus) yang Dijual di Beberapa Pasar Daerah Kabupaten Badung, Bali Kadek Aryndha Sukma Prabaswari; Retno Kawuri; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p4

Chicken meat is a source of animal protein which is easily damaged due to its high water content. Chicken meat that is bought and sold could potentially become a place for the growth of bacteria that cause foodborne diseases.This study aims to determine total bacterial contamination, Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat in Badung Regency and determine the suitability of broiler chicken meat samples following food safety standards. Samples were taken using a purposive sampling method in six sub-districts in Badung Regency, namely Petang, Abiansemal, Mengwi, North Kuta, Kuta and South Kuta sub-districts at traditional markets that sell broiler chicken meat. The samples were tested using the pour plate and streak platting method. Parameters tested include Total Plate Number (ALT), Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat samples. Coliform and Escherichia coli bacteria were detected using Petrifilm ready-to-use media, while Salmonella sp. tested on selective Salmonella-Shigella Agar (SSA) media and catalase test and Gram staining. The research result showed that the contamination rates in broiler chicken breast meat were: Total Plate Number (ALT) 105 CFU/g to 107 CFU/g; Coliform and Escherichia coli 101 CFU/g to 104 CFU/g; Salmonella sp. positive in all samples, so that the samples tested did not meet the safety quality requirements for chicken meat according to SNI 3924: 2009.

Eksplorasi Streptomyces Sp. Di Hutan De Djawatan Banyuwangi Dan Aktivitasnya Dalam Menghambat Candida albicans Putu Anggita Sekar Pratiwi; Retno Kawuri; I Ketut Muksin
Metamorfosa: Journal of Biological Sciences Vol. 13 No. 1 (2026)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2026.v13.i01.p1

Kandidiasis merupakan penyakit opportunistik akibat infeksi jamur Candida albicans yang menyerangmembran mukosa. Solusi untuk mengatasi adalah dengan memanfaatkan mikroorganisme penghasilsenyawa bioaktif berupa imunospresan, antibiotik, antijamur, antiinflamasi dari bakteri genusStreptomyces. Penelitian ini bertujuan untuk mengeksplorasi potensi Streptomyces sp. penghasilsenyawa bioaktif di hutan De Djawatan Banyuwangi dalam menghambat jamur C. albicans. Metodepenelitian mencakup pengambilan sampel tanah dari hutan De Djawatan, isolasi bakteri Streptomycessp. menggunakan plating method, serta identifikasi makroskopik dan mikroskopik. Uji daya hambatterhadap jamur patogen C. albicans dilakukan dengan metode dual culture. Hasil eksplorasi di hutan DeDjawatan Banyuwangi memperoleh total sebanyak 17 isolat dengan Streptomyces sp.7 memiliki dayahambat tertinggi sebesar 1,375 cm, sedangkan daya hambat terendah dimiliki oleh Streptomyces sp.12sebesar 0,783 cm terhadap C. albicans. Penelitian ini mengimplikasikan bahwa hutan De DjawatanBanyuwangi berpotensi sebagai sumber Streptomyces sp. penghasil senyawa antijamur yangmendukung eksplorasi agen alami untuk mengatasi infeksi C. albicans.Kata kunci: Actinobacteria, antifungi, biokontrol, metabolit sekunder

Eksplorasi Streptomyces Sp. Di Hutan De Djawatan Banyuwangi Dan Aktivitasnya Dalam Menghambat Candida albicans Putu Anggita Sekar Pratiwi; Retno Kawuri; I Ketut Muksin
Metamorfosa: Journal of Biological Sciences Vol. 13 No. 1 (2026)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2026.v13.i01.p1

Kandidiasis merupakan penyakit opportunistik akibat infeksi jamur Candida albicans yang menyerangmembran mukosa. Solusi untuk mengatasi adalah dengan memanfaatkan mikroorganisme penghasilsenyawa bioaktif berupa imunospresan, antibiotik, antijamur, antiinflamasi dari bakteri genusStreptomyces. Penelitian ini bertujuan untuk mengeksplorasi potensi Streptomyces sp. penghasilsenyawa bioaktif di hutan De Djawatan Banyuwangi dalam menghambat jamur C. albicans. Metodepenelitian mencakup pengambilan sampel tanah dari hutan De Djawatan, isolasi bakteri Streptomycessp. menggunakan plating method, serta identifikasi makroskopik dan mikroskopik. Uji daya hambatterhadap jamur patogen C. albicans dilakukan dengan metode dual culture. Hasil eksplorasi di hutan DeDjawatan Banyuwangi memperoleh total sebanyak 17 isolat dengan Streptomyces sp.7 memiliki dayahambat tertinggi sebesar 1,375 cm, sedangkan daya hambat terendah dimiliki oleh Streptomyces sp.12sebesar 0,783 cm terhadap C. albicans. Penelitian ini mengimplikasikan bahwa hutan De DjawatanBanyuwangi berpotensi sebagai sumber Streptomyces sp. penghasil senyawa antijamur yangmendukung eksplorasi agen alami untuk mengatasi infeksi C. albicans.Kata kunci: Actinobacteria, antifungi, biokontrol, metabolit sekunder

Phenotypic evaluation of gamma-induced dwarf mutants in Dendrobium crumenatum at M2 generation DWIYANI, RINDANG; DARMAWATI, IDA AYU PUTRI; KAWURI, RETNO; FITRIANI, YUYUN; KAMUHI, RINI HERMINA; HANIFAH, WAFA’ NUR; SANTIKA, I PUTU BENNY
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100150

Abstract. Dwiyani R, Darmawati IAP, Kawuri R, Fitriani Y, Kamuhi RH, Hanifah WN, Santika IPB. 2026. Phenotypic evaluation of gamma-induced dwarf mutants in Dendrobium crumenatum at M2 generation. Asian J Agric 10 (1): g100150. https://doi.org/10.13057/asianjagric/g100150. Bali, Indonesia, has strong potential for the development of ornamental orchids, particularly compact cultivars with high commercial value. Dendrobium crumenatum, a native Indonesian orchid, is widely appreciated for its white, fragrant flowers; however, its tall growth habit limits its suitability as a potted ornamental plant. This study aimed to induce and evaluate dwarf phenotypes in D. crumenatum using gamma irradiation. Plantlets were exposed to 0, 5, 10, 15, and 20 Gy, arranged in a completely randomized design with five replicates and three plantlets per replicate (n=75). Morphological traits were assessed after in vitro culture and acclimatization. Data were analyzed using analysis of variance followed by the Least Significant Difference (LSD) test at a 5% significance level (p<0.05). Gamma irradiation significantly affected all measured traits. The 5 Gy treatment caused severe growth suppression, reducing plant height, leaf number, and shoot number by approximately 53.7%, 81.7%, and 84.4%, respectively, compared to the control. In contrast, the 10 Gy treatment achieved a similar reduction in plant height (~52.6%) while maintaining higher leaf number (~83.3% of control) and shoot number (~62.2% of control), indicating a more balanced morphological response. The 15 Gy treatment showed minimal deviation from the control, whereas 20 Gy produced semi-compact plants with moderate shoot retention. These results demonstrate a non-linear dose-response relationship, where intermediate doses (particularly 10 Gy) optimize the trade-off between dwarfism and vegetative performance. These findings indicate that medium gamma irradiation doses are effective for generating dwarf D. crumenatum mutants suitable for potted ornamental use and demonstrate the potential of mutation breeding to support orchid improvement and the floriculture industry.

Title

Found 19 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 19 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 19 Documents
Search