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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Biosains Pascasarjana JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) ARSHI Veterinary Letters JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Jurnal Pengabdian UNDIKMA Bioscientist : Jurnal Ilmiah Biologi Jurnal Ilmiah Sangkareang Mataram Journal Transformation of Mandalika MANDALIKA VETERINARY JOURNAL Bakti Sekawan : Jurnal Pengabdian Masyarakat Biocaster : Jurnal Kajian Biologi Journal of Livestock and Animal Health (JLAH) Buletin Pengabdian Jurnal Info Kesehatan
. Kholik
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Detection of HSP70 in Working Horses Using ELISA on Sumbawa Island Haddar, Mariyam Al; Pradana, Munawer; Kholik, Kholik; Supriadi, Supriadi; Satuman, Satuman
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15197

Abstract

Heat shock protein 70 also known as HSP70 is a protein that regulates in response to stress factors such as heat, exercise, and others. The current research work used enzyme-linked immunosorbent assay (ELISA) to measure HSP70 levels in working horses on Sumbawa Island. Blood was taken through jugular venipuncture whereas saliva was collected non-invasively via cotton swab, both were analyzed with ELISA. The results indicated different expressions of HSP70; saliva shows a significantly higher level of HSP70 (52.07 ± 25.25 ng/mL) than serum (43.71 ± 34.83 ng/mL) from the same animal. The observed differences might indicate a better representation of acute stress by saliva due to physiological rapid turnover compared to serum, which reflects an integration of cumulative stress exposure. Our finding thus proves that double monitoring of both biological matrices offers complementary information in assessing stress in working horses while taking salivary HSP70 into account as especially promising for the in-field welfare monitoring due to its non-invasive collection and sensitivity to immediate stress conditions.
The Density of Aedes aegypti Larvae Density and the Incidence of Dengue Hemorrhagic Fever in the Pesingahan Environment, Pagesangan Barat Village, Mataram Urip, Urip; Rizki, Kurnia; Tatontos, Erlin Yustin; Gunarti, Gunarti; Kholik, Kholik
JURNAL INFO KESEHATAN Vol 21 No 1 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss1.986

Abstract

Dengue hemorrhagic fever remains a public health concern in West Nusa Tenggara Province, with the majority of cases occurring in Mataram City and the Pesinggahan Environment of West Pagesangan Village. The density of Aedes aegypti larvae as disease vectors has a strong influence on the high incidence of cases and the spread of dengue hemorrhagic fever. The objective of this study was to identify if there was a relation between the density of Aedes aegypti larvae and the rate of dengue hemorrhagic fever in the Pesinggahan Environment of Pagesangan Barat Village. This study is an analytic observational study with a cross-sectional design. This study's sample size was 56 houses. The data collected included information on the density of Aedes aegypti larvae and the incidence rate of dengue hemorrhagic fever in the Pesinggahan Environment of Pagesangan Barat Village over the previous year. Descriptive analysis was used to examine data on the density of Aedes aegypti larvae and the incidence rate of dengue hemorrhagic fever in the Pesinggahan environment. The findings revealed that the density of Aedes aegypti larvae was high (Density Figure (DF) = 6), as was the incidence rate of dengue hemorrhagic fever (0.39%). The statistical analysis between the density of A. aegypti larvae and the incidence rate of dengue hemorrhagic fever in the Pesinggahan Environment with the chi square test demonstrated indicate that p-value = 0.000 < alpha = 0.05. Based on the results of the study, there was a relationship between the density of A. aegypti larvae and the incidence rate of dengue hemorrhagic fever in Pesinggahan, Pagesangan Barat Village. Further research is required regarding the detection of the dengue virus transmitted by A. aegypti mosquitoes to their offspring (transovarial).
Pemeriksaan Ante dan Post Mortem Hewan Kurban di Musholla Al Atqiyah Kota Mataram Kholik, Kholik; Pradana, Munawer; Riwu, Katty Hendriana Priscilia; Kurnia, Rofi; Pratiwi, Dhea Siskha
Bakti Sekawan : Jurnal Pengabdian Masyarakat Vol. 5 No. 2 (2025): Desember
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/bakwan.v5i2.875

Abstract

The Faculty of Veterinary Medicine, UNDIKMA, in collaboration with the Al-Atqiyah Mosque in Mataram City, conducted an inspection of sacrificial animals on Eid al-Adha. The inspection aims to provide a sense of security to the public with ASUH meat products. The inspection of sacrificial animals includes a health inspection before slaughter (ante-mortem) and then continued with an inspection after the sacrificial animals are slaughtered (post-mortem). The inspection of sacrificial animals was carried out from June 6 to 7, 2025. The inspection of sacrificial animals was carried out on 14 sacrificial animals consisting of 3 cows and 11 goats. The ante-mortem inspection found that the 14 sacrificial animals were in healthy condition. The results of the post-mortem examination of the liver, heart, spleen, lungs, and kidneys did not find any organ changes and lesions from the characteristics of infectious or zoonotic diseases. In the post-mortem examination, only Fasciola sp worms were found in 15.38% of the 14 sacrificial animals examined. Fasciola sp was found in one cow (33.33%) of the 3 cows examined and Fasciola sp was also found in only one goat (9.09%) of the 11 goats examined, so the sacrificial meat is suitable for public consumption.
Pengaruh Ekstrak Bawang Merah (Allium cepa L.) terhadap Gambaran Leukosit Tikus Wistar yang Diinfeksi Staphylococcus aureus Munawaroh, Muhammad; Angkasa, Winoto; Santosa, Christin Marganingsih; Kholik, Kholik; Rosyadi, Imron
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.44892

Abstract

Shallot (Allium cepa L) has been used emperically to treat wounds, bloat, common cold and is known to boost immunity. Shallots have a variety of useful compounds such as flavonoids, antioxidants, quercetin, saponins and many other compounds. These compounds have been known to have antibacterials, antihistamines, and immunomodulators activities. This study aimed to examine the  potential of shallot extract in wounded rats that are infected with Staphylococcus aureus.Fifteen female Wistar rat were divided into 5 treatment groups: 40% shallot extract, 80% shallot extract, 10% povidone iodine, positive control and negative control. The rats were wounded by making an incision on the back then the shallot extract was administered topically once a day for 21 days. Blood samples were taken on days 0 and 21 then the total number of leukocytes and its differentials     (neutrophils, lymphocytes, monocytes, eosinophils and basophils) were calculated. The data were then processed using one-way ANOVA test with 95% significance value.The result showed a significant increase (p<0,05) on the total number of leukocyte (10,37 ± 1,09 103/µl), neutrophils (4,98 ± 0,30 103/µl), lymphocytes  (8,76 ± 0,04 103/µl) and monocytes (0,74 ± 0,02 103/µl) on the group treated with 40% shallot extract. While on the group treated with 80% shallot extract there was a significant increase (p<0,005) on the total number of leukocytes (10,40 ± 0,43 103/µl), lymphocytes (7,67 ± 0,19 103/µl), and monocytes (0,64 ± 0,01 103/µl). This study concluded that shallot extract is able to improve the immune system.
Resistensi Antibiotik Bakteri Escherichia coli dari Air Sumur di Peternakan Ayam Desa Suranadi, Lombok Barat Sopiandi, Sopiandi; Kholik, Kholik; Atma, Candra Dwi; Pradana, Munawer; Hendarti, Gracia Angelina
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1127

Abstract

Antimicrobial Resistance (AMR) has become a global health problem. Escherichia coli bacteria in water can act as a reservoir for the spread of antibiotic resistance. The study aimed to determine the resistance of Escherichia coli isolated from well water in chicken farms to antibiotics. The study design was an observational survey conducted from November to December 2022 at five chicken farms in Suranadi Village, Narmada District, West Lombok Regency. Five well water samples used in the study came from five wells in chicken farms located 10 meters from the chicken coop. 250 ml of well water samples were taken, put into sterile bottles, and then taken to the Health Laboratory Testing and Calibration Center of West Nusa Tenggara Province for isolation of Escherichia coli. Escherichia coli was isolated by planting on Eosin Methylene Blue Agar (EMBA) and identified using gram staining and biochemical tests. Antibiotic susceptibility testing of Escherichia coli was conducted using the disc diffusion method using five antibiotics: Gentamicin, Tetracycline, Amoxycillin, Chloramphenicol, and Ciprofloxacin. This study successfully isolated four Escherichia coli from five well water samples. The susceptibility test results showed that 75% of the Escherichia coli isolates were resistant to Amoxycillin, 50% to Ciprofloxacin, and 25% to Chloramphenicol. The Escherichia coli isolates were still sensitive to Gentamicin and Tetracycline.
Deteksi mastitis subklinis pada kambing peranakan etawa di hanif fram dusun aik mual timur desa aik mual kecamatan praya kabupaten lombok tengah provinsi nusa tenggara barat Mardani; Kholik; Dina Oktaviana; Maratun Janah
Journal Transformation of Mandalika, e-ISSN: 2745-5882, p-ISSN: 2962-2956 Vol. 7 No. 5 (2026): Mei
Publisher : Institut Penelitian dan Pengembangan Mandalika Indonesia (IP2MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/jtm.v7i5.6410

Abstract

The Etawa crossbred goat is a hybrid goat that plays a strategic role in the Indonesian livestock sector. A cross between the native Etawa goat from India and local Indonesian goats, this species has undergone a historical evolution that reflects the adaptation of modern livestock technology to tropical environments. Mastitis occurs when pathogens enter the udder through the milk ducts, from unhygienic milking processes, or wounds in the udder skin. The main causes are bacteria such as Staphylococcus aureus, Streptococcus spp., or Escherichia coli. The purpose of this study was to determine the detection of subclinical mastitis in Etawa crossbred goats and to determine the California Mastitis Test (CMT) score. It can be concluded that an average of 16 Etawa crossbred goat tail samples (lactaxes) were found to be 14 goat tail samples that were indicated positive for subclinical mastitis, with a detection rate of 87.5%. Subclinical mastitis is caused by several factors, including poor barn management, the age of the lactation and sow, and poor hygiene during milking, which can lead to contamination of healthy goats from sick PE goats.
Co-Authors Afnani, Daniah Ashri Akhmad Sukri Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alza Hamdi Putra Andreayani Attamimi Andreyani Attamimi Angkasa, Winoto Aprianti, Aurira Thrisma Dwi Ariandoko Ariandoko Asmara, Zinie Hikmah Atma, Candra Dwi Barmawi, Muhammad Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Christin Marganingsih Santosa Dedi Putra Dita Deta Ramdani Qadriyah Dharmawibawa, Iwan Doddy Dina Oktaviana Dina Oktaviana Dina Oktaviana Dina Oktaviana Dina Wurinaharuma Dwipal Tio Ikhsan Hadi Erlin Yustin Tatontos Erni Fadilah, Aulia Rizki Fauzi Saputra Fernando Jose Immanuel Clinton Situmorang Garnika, Eneng Gunarti Gunarti Gunarti, Gunarti Gunawan Gunawan Gunawan Hamzah, Arshq Mirza Hendarti, Gracia Angelina Humairah, Ira Husnaini, Farhan Imron Rosyadi Ismail Marzuki Iwan Desimal Iwan Doddy Dharmawibawa Jayanti Mandrasari Kadek Rachmawati Karina, Soca Karinda, Kisvan Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Khairullah, Aswin Rafif Kristian Wolagole Kunti Tirtasari Kunti Tirtasari Kurnia Rizki Kurnia, Rofi Kurniawan, Shendy Canadya Lalu Faesal Fajri Lalu Purnama Tasyakusuma Lukitasari, Lina Maratun Janah Maratun Janah, Maratun Maratun Jannah Mardani Mariyam Al Haddar Mashur Mashur Mbura, Yonanda Verawati Haslinda Moch Taufik Hidayatullah Muhammad Hidayat Muhammad Munawaroh Muhammad Munawaroh Muhammad Munawaroh Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Mujmal, Khaerul Munawaroh, Muhammad Munawer Pradana Ni Ketut Alit Suarti Ningrum, Adek Livia Yunita Nofisulastri, Nofisulastri Nova Kurnia Novarina Sulsia Novarina Sulsia Ista&#039;In Ningytas Novarina Sulsia Ista'In Ningtyas Nur Rusdiana Nurul Khairah Prabowo, Gwenny Ichsan Pradana, Munawer Pratiwi, Dhea Siskha Pudji Srianto Purwanti, Ni Luh Lasmi Qurnianingsih, Ema Rahmawati Rahmawati Rahmawati, Septiyana Eka Rahmawati, Septyana Eka Rahmawati, Siti Irma Ramdani, Fitrah Akbar Rima Nurmayani Riwu, Katty Hedriana Priscilia Riwu, Katty Hendriana Priscilia Riwu, Katty Hendriana Priscillia Rizki, Kurnia Rusyda, Aqila sabila Sabaruddin Safitri, Indi Yulia Satuman Satuman Sa’diyah, Siti Nurus Seli Nurmayani Septyana Eka Rahmawati Siti Khaerunnisa Siti Nurus Sa’diyah Sopiandi, Sopiandi Supriadi Supriadi Supriadi Supriadi Supriadi Syafindri syafindri syafindri Tirtasari, Kunti Ummah, Fithriyah Cholifatul Ummah Urip Urip Urip Urip, Urip wijaya, I Gusti Made Arjana Wiwik Handayani Wungu, Citrawati Dyah Kencono Yeti Kurniasih Yumi Sartika Zakarias, Herdin Vanek