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Pengaruh Ekstrak Bawang Merah (Allium cepa L.) terhadap Gambaran Leukosit Tikus Wistar yang Diinfeksi Staphylococcus aureus Munawaroh, Muhammad; Angkasa, Winoto; Santosa, Christin Marganingsih; Kholik, Kholik; Rosyadi, Imron
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.44892

Abstract

Shallot (Allium cepa L) has been used emperically to treat wounds, bloat, common cold and is known to boost immunity. Shallots have a variety of useful compounds such as flavonoids, antioxidants, quercetin, saponins and many other compounds. These compounds have been known to have antibacterials, antihistamines, and immunomodulators activities. This study aimed to examine the  potential of shallot extract in wounded rats that are infected with Staphylococcus aureus.Fifteen female Wistar rat were divided into 5 treatment groups: 40% shallot extract, 80% shallot extract, 10% povidone iodine, positive control and negative control. The rats were wounded by making an incision on the back then the shallot extract was administered topically once a day for 21 days. Blood samples were taken on days 0 and 21 then the total number of leukocytes and its differentials     (neutrophils, lymphocytes, monocytes, eosinophils and basophils) were calculated. The data were then processed using one-way ANOVA test with 95% significance value.The result showed a significant increase (p<0,05) on the total number of leukocyte (10,37 ± 1,09 103/µl), neutrophils (4,98 ± 0,30 103/µl), lymphocytes  (8,76 ± 0,04 103/µl) and monocytes (0,74 ± 0,02 103/µl) on the group treated with 40% shallot extract. While on the group treated with 80% shallot extract there was a significant increase (p<0,005) on the total number of leukocytes (10,40 ± 0,43 103/µl), lymphocytes (7,67 ± 0,19 103/µl), and monocytes (0,64 ± 0,01 103/µl). This study concluded that shallot extract is able to improve the immune system.
Deteksi mastitis subklinis pada kambing peranakan etawa di hanif fram dusun aik mual timur desa aik mual kecamatan praya kabupaten lombok tengah provinsi nusa tenggara barat Mardani; Kholik; Dina Oktaviana; Maratun Janah
Journal Transformation of Mandalika, e-ISSN: 2745-5882, p-ISSN: 2962-2956 Vol. 7 No. 5 (2026): Mei
Publisher : Institut Penelitian dan Pengembangan Mandalika Indonesia (IP2MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/jtm.v7i5.6410

Abstract

The Etawa crossbred goat is a hybrid goat that plays a strategic role in the Indonesian livestock sector. A cross between the native Etawa goat from India and local Indonesian goats, this species has undergone a historical evolution that reflects the adaptation of modern livestock technology to tropical environments. Mastitis occurs when pathogens enter the udder through the milk ducts, from unhygienic milking processes, or wounds in the udder skin. The main causes are bacteria such as Staphylococcus aureus, Streptococcus spp., or Escherichia coli. The purpose of this study was to determine the detection of subclinical mastitis in Etawa crossbred goats and to determine the California Mastitis Test (CMT) score. It can be concluded that an average of 16 Etawa crossbred goat tail samples (lactaxes) were found to be 14 goat tail samples that were indicated positive for subclinical mastitis, with a detection rate of 87.5%. Subclinical mastitis is caused by several factors, including poor barn management, the age of the lactation and sow, and poor hygiene during milking, which can lead to contamination of healthy goats from sick PE goats.
Deteksi Resistensi Isolat Bakteri Salmonella sp. dari Feses Segar Ayam Petelur (Gallus gallus domesticus) terhadap Antibiotik Widia Restu; Candra Dwi Atma; Yeti Kurniasih; Kholik Kholik
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 3 (2026): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i3.1199

Abstract

Antibiotic resistance is a global problem that connects human and animal health. This resistance occurs when bacteria acquire genes that allow them to survive when exposed to antibiotics. Wanasaba District, East Lombok is one of the districts that has a large population of laying hens, one of the infectious diseases that often attacks poultry and is treated using antibiotics is salmonella sp. This study aims to detect the resistance of Salmonella sp. bacterial isolates derived from fresh feces of laying hens (Gallus gallus domesticus) to antibiotics. This study is a laboratory experimental study conducted in November 2025 with a total of 3 samples of fresh feces of laying hens taken using a purposive sampling method. Bacterial isolation was carried out using Xylose Lysine Deoxycholate (XLD) agar media, then identified using biochemical tests to confirm the presence of Salmonella sp. Antibiotic resistance tests were carried out using the Kirby-Bauer disc diffusion method using 30 μg tetracycline antibiotics, 10 μg gentamicin, and 5 μg enrofloxacin. The results showed that all Salmonella sp. isolates from the three samples demonstrated 100% sensitivity to the three types of antibiotics tested. These results indicate that Salmonella sp. isolates in laying hens at the study site have not yet developed resistance to the antibiotics used. However, monitoring antibiotic use and implementing good farm management are still necessary to prevent the emergence of bacterial resistance in the future.
Resistensi Antibiotik Bakteri Escherichia coli dari Air Sumur di Peternakan Ayam Desa Suranadi, Lombok Barat Sopiandi Sopiandi; Kholik Kholik; Candra Dwi Atma; Munawer Pradana; Gracia Angelina Hendarti
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1127

Abstract

Antimicrobial Resistance (AMR) has become a global health problem. Escherichia coli bacteria in water can act as a reservoir for the spread of antibiotic resistance. The study aimed to determine the resistance of Escherichia coli isolated from well water in chicken farms to antibiotics. The study design was an observational survey conducted from November to December 2022 at five chicken farms in Suranadi Village, Narmada District, West Lombok Regency. Five well water samples used in the study came from five wells in chicken farms located 10 meters from the chicken coop. 250 ml of well water samples were taken, put into sterile bottles, and then taken to the Health Laboratory Testing and Calibration Center of West Nusa Tenggara Province for isolation of Escherichia coli. Escherichia coli was isolated by planting on Eosin Methylene Blue Agar (EMBA) and identified using gram staining and biochemical tests. Antibiotic susceptibility testing of Escherichia coli was conducted using the disc diffusion method using five antibiotics: Gentamicin, Tetracycline, Amoxycillin, Chloramphenicol, and Ciprofloxacin. This study successfully isolated four Escherichia coli from five well water samples. The susceptibility test results showed that 75% of the Escherichia coli isolates were resistant to Amoxycillin, 50% to Ciprofloxacin, and 25% to Chloramphenicol. The Escherichia coli isolates were still sensitive to Gentamicin and Tetracycline.
Deteksi dan Identifikasi Telur Cacing Nematoda pada Feses Sapi Bali (Bos sondaicus) di Desa Mumbul Sari Kecamatan Bayan Kabupaten Lombok Utara Khaerul Mujmal; Iwan Doddy Dharmawibawa; Kholik Kholik; Maratun Janah
Biocaster : Jurnal Kajian Biologi Vol. 5 No. 4 (2025): October
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v5i4.643

Abstract

Indonesia is a country rich in natural resources, including in the field of livestock, one of which is cattle. Cows have an important role as providers of meat needs for the community. However, cows are also at risk of being infected with worms that can reduce immunity, inhibit weight gain, and potentially cause death. The purpose of this study is to detect and identify nematode worm eggs in the feces of Balinese cows (Bos sondaicus) located in Mumbul Sari Village, North Lombok Regency. The sampling method in this study uses the purposive sampling method based on the maintenance system. Sampling of Balinese cow feces (Bos sondaicus) was taken in Mumbul Sari Village, North Lombok Regency, by taking fresh cow feces, then taken to the Parasitology Laboratory, Faculty of Veterinary Medicine, Mandalika Education University, to be examined using the saturated sugar flotation method, then observed under a microscope with a magnification of 100 times. The results of the study showed positive examination, namely the presence of Trichostrongylus sp; Toxocara vitulorum sp; Oesophagostomum sp; and Haemonchus contortus sp; on the feces of Balinese cattle from Mumbul Sari Hamlet, Bagek Nunggal, Pawang Kunyit I, Pawang Kunyit II, and Munder Hamlet.
MULTIDRUG RESISTANCE (MDR) Escherichia coli BERSUMBER FESES SEGAR KUDA CIDOMO DI PASAR AMPENAN KOTA MATARAM Katty Hendriana Priscilia Riwu; Ichwan Ramdhani Hamdan Umar; Iwan Doddy Dharmawibawa; Kholik Kholik
Jurnal Veteriner Nusantara Vol 9 No 2 (2026): Agustus, 2026
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v9i2.26866

Abstract

Escherichia coli is a normal commensal bacterium in the gastrointestinal tract of animals; however, some strains may become pathogenic and develop antimicrobial resistance. This study aimed to identify E. coli from the feces of Cidomo horses at Kebon Roek Market, Ampenan, and to determine their antibiotic resistance profile, including the presence of multidrug-resistant (MDR) isolates. A total of 36 fresh fecal samples were collected purposively and cultured on Eosin Methylene Blue Agar (EMBA), followed by Gram staining and biochemical tests for confirmation. Antimicrobial susceptibility was assessed using the disk diffusion method against streptomycin, aztreonam, tetracycline, ciprofloxacin, and trimethoprim–sulfamethoxazole, and interpreted according to CLSI (2020) guidelines. MDR isolates were further tested using the Double Disk Synergy Test (DDST) to detect Extended Spectrum Beta-Lactamase (ESBL) production. The results showed that 7 of 36 samples (19.44%) were confirmed as E. coli. High resistance levels were observed against streptomycin and aztreonam, whereas tetracycline and ciprofloxacin exhibited the highest effectiveness. Three isolates (42.85%) were classified as MDR, displaying resistance to ≥3 antibiotic classes. All MDR isolates tested negative for ESBL production using DDST. These findings indicate that Cidomo horses may act as reservoirs of antibiotic-resistant E. coli in market environments. Strengthening hygiene management and promoting prudent antimicrobial use in animals are essential to reduce the risk of antimicrobial resistance transmission within the One Health framework.
Detection of Anisakis sp. in Eastern Little Tuna (Euthynnus Affinis) from Traditional Markets in Mataram Using The Enzymatic Digestion Method Mustika Jalianti Hasan; Kholik Kholik; Katty Hendriana Priscilia Riwu; Candra Dwi Atma; Freshindy Marissa Wibisono
Journal of Parasite Science Vol. 10 No. 2 (2026): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v10i2.96226

Abstract

Eastern little tuna (Euthynnus affinis) is a widely consumed marine fish in Indonesia and is known to serve as a paratenic host for zoonotic parasites. This study aimed to detect the presence of Anisakis sp. larvae in E. affinis obtained from two traditional fish markets in Mataram, West Nusa Tenggara, Indonesia: Traditional Kebon Roek Market and Bintaro Fish Market. A total of 50 fish samples were collected using simple random sampling. Enzymatic digestion method was applied to detect Anisakis sp. larvae from visceral organs and muscle tissue separately. Morphological identification was performed under stereo and compound microscopy at 10× magnification. Results showed that 5 of 50 samples (10%) were positive for Anisakis sp. larvae, with a mean intensity of 1 larva per infected fish. All positive larvae were recovered exclusively from visceral organs, while no larvae were detected in muscle tissue. Morphological examination confirmed the larvae as Anisakis sp. Type I, characterized by the presence of a boring tooth, elongated ventriculus, and mucron at the caudal end. These findings demonstrate that E. affinis traded in traditional markets of Mataram carries Anisakis sp. at a relatively low infestation level, yet poses a potential zoonotic risk. Proper post-harvest handling and adequate cooking are essential to prevent anisakiasis in consumers.
An Incidence of Repeat Breeding in Female Bali Cattle (Bos sondaicus) in Bayan District, North Lombok, Indonesia Farizi, Dicki Al; Kholik; Lovela, Aprinda Ratna; Mudhita , Ida Ketut
Veterinary Biomedical and Clinical Journal Vol. 8 No. 1 (2026): Vol. 8 No. 1 2026
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2026.008.01.4

Abstract

Repeat breeding is one of the reproductive disorders that can reduce reproductive efficiency and productivity in cattle, including female Bali cattle (Bos sondaicus). Bayan District, North Lombok Regency, is an area with a relatively high population of Bali cattle, where repeated pregnancy failure following artificial insemination has still been reported. This study aimed to determine the incidence of repeat breeding in female Bali cattle in Bayan District, North Lombok, Indonesia. The study was conducted using a survey method with a quantitative descriptive approach. The research sample consisted of 68 multiparous female Bali cattle aged 3–7 years, selected using purposive sampling. Primary data were obtained through field observations and interviews with farmers, while secondary data were collected from artificial insemination records available at the UPTD of the Department of Food Security, Fisheries, and Agriculture of Bayan District, North Lombok. Repeat breeding was defined as a condition in which female cattle failed to conceive after three or more inseminations or matings. The results showed that 13 female Bali cattle (19.1%) experienced repeat breeding, while 55 cattle (80.9%) successfully conceived. These findings indicate that the incidence of repeat breeding in female Bali cattle in the study area is categorized as moderate and may potentially reduce reproductive efficiency if not properly managed. Therefore, continuous monitoring of reproductive performance and improvement of reproductive management are necessary to reduce the incidence of repeat breeding and enhance the productivity of Bali cattle.
Co-Authors Afnani, Daniah Ashri Akhmad Sukri Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alza Hamdi Putra Andreayani Attamimi Andreyani Attamimi Angkasa, Winoto Aprianti, Aurira Thrisma Dwi Ariandoko Ariandoko Asmara, Zinie Hikmah Atma, Candra Dwi Barmawi, Muhammad Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Christin Marganingsih Santosa Dedi Putra Dita Deta Ramdani Qadriyah Dharmawibawa, Iwan Doddy Dina Oktaviana Dina Oktaviana Dina Oktaviana Dina Oktaviana Dina Wurinaharuma Dwipal Tio Ikhsan Hadi Erlin Yustin Tatontos Erni Fadilah, Aulia Rizki Farizi, Dicki Al Fauzi Saputra Fernando Jose Immanuel Clinton Situmorang Freshindy Marissa Wibisono Garnika, Eneng Gracia Angelina Hendarti Gunarti Gunarti Gunarti, Gunarti Gunawan Gunawan Gunawan Hidayatullah, Moch Taufik Husnaini, Farhan Ichwan Ramdhani Hamdan Umar Imron Rosyadi Ismail Marzuki Iwan Desimal Iwan Doddy Dharmawibawa Iwan Doddy Dharmawibawa Jayanti Mandrasari Karina, Soca Karinda, Kisvan Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Khaerul Mujmal Khairullah, Aswin Rafif Kristian Wolagole Kunti Tirtasari Kunti Tirtasari Kurnia Rizki Kurnia, Rofi Lalu Faesal Fajri Lalu Purnama Tasyakusuma Lovela, Aprinda Ratna Maratun Janah Maratun Janah Maratun Jannah Mardani Mariyam Al Haddar Mashur Mashur Mbura, Yonanda Verawati Haslinda Mudhita , Ida Ketut Muhammad Munawaroh Muhammad Munawaroh Muhammad Munawaroh Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Munawaroh, Muhammad Munawer Pradana Munawer Pradana Mustika Jalianti Hasan Ni Ketut Alit Suarti Ningrum, Adek Livia Yunita Nofisulastri, Nofisulastri Nova Kurnia Novarina Sulsia Novarina Sulsia Ista&#039;In Ningytas Novarina Sulsia Ista'In Ningtyas Nur Rusdiana Nurul Khairah Pradana, Munawer Pratiwi, Dhea Siskha Pudji Srianto Purwanti, Ni Luh Lasmi Qodri, Hasby faizul Rahmawati Rahmawati Rahmawati, Septiyana Eka Rahmawati, Septyana Eka Rahmawati, Siti Irma Ramdani, Fitrah Akbar Rima Nurmayani Riwu, Katty Hedriana Priscilia Riwu, Katty Hendriana Priscilia Riwu, Katty Hendriana Priscillia Rizki, Kurnia Sabaruddin Satuman Satuman Sa’diyah, Siti Nurus Seli Nurmayani Septyana Eka Rahmawati Siti Nurus Sa’diyah Sopiandi Sopiandi Supriadi Supriadi Supriadi Supriadi Supriadi Syafindri syafindri syafindri Tirtasari, Kunti Urip Urip Urip Urip, Urip Widia Restu wijaya, I Gusti Made Arjana Wiwik Handayani Yeti Kurniasih Yeti Kurniasih Yumi Sartika Zakarias, Herdin Vanek