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Pengaruh Ekstrak Bawang Merah (Allium cepa L.) terhadap Gambaran Leukosit Tikus Wistar yang Diinfeksi Staphylococcus aureus Munawaroh, Muhammad; Angkasa, Winoto; Santosa, Christin Marganingsih; Kholik, Kholik; Rosyadi, Imron
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.44892

Abstract

Shallot (Allium cepa L) has been used emperically to treat wounds, bloat, common cold and is known to boost immunity. Shallots have a variety of useful compounds such as flavonoids, antioxidants, quercetin, saponins and many other compounds. These compounds have been known to have antibacterials, antihistamines, and immunomodulators activities. This study aimed to examine the  potential of shallot extract in wounded rats that are infected with Staphylococcus aureus.Fifteen female Wistar rat were divided into 5 treatment groups: 40% shallot extract, 80% shallot extract, 10% povidone iodine, positive control and negative control. The rats were wounded by making an incision on the back then the shallot extract was administered topically once a day for 21 days. Blood samples were taken on days 0 and 21 then the total number of leukocytes and its differentials     (neutrophils, lymphocytes, monocytes, eosinophils and basophils) were calculated. The data were then processed using one-way ANOVA test with 95% significance value.The result showed a significant increase (p<0,05) on the total number of leukocyte (10,37 ± 1,09 103/µl), neutrophils (4,98 ± 0,30 103/µl), lymphocytes  (8,76 ± 0,04 103/µl) and monocytes (0,74 ± 0,02 103/µl) on the group treated with 40% shallot extract. While on the group treated with 80% shallot extract there was a significant increase (p<0,005) on the total number of leukocytes (10,40 ± 0,43 103/µl), lymphocytes (7,67 ± 0,19 103/µl), and monocytes (0,64 ± 0,01 103/µl). This study concluded that shallot extract is able to improve the immune system.
Deteksi mastitis subklinis pada kambing peranakan etawa di hanif fram dusun aik mual timur desa aik mual kecamatan praya kabupaten lombok tengah provinsi nusa tenggara barat Mardani; Kholik; Dina Oktaviana; Maratun Janah
Journal Transformation of Mandalika, e-ISSN: 2745-5882, p-ISSN: 2962-2956 Vol. 7 No. 5 (2026): Mei
Publisher : Institut Penelitian dan Pengembangan Mandalika Indonesia (IP2MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/jtm.v7i5.6410

Abstract

The Etawa crossbred goat is a hybrid goat that plays a strategic role in the Indonesian livestock sector. A cross between the native Etawa goat from India and local Indonesian goats, this species has undergone a historical evolution that reflects the adaptation of modern livestock technology to tropical environments. Mastitis occurs when pathogens enter the udder through the milk ducts, from unhygienic milking processes, or wounds in the udder skin. The main causes are bacteria such as Staphylococcus aureus, Streptococcus spp., or Escherichia coli. The purpose of this study was to determine the detection of subclinical mastitis in Etawa crossbred goats and to determine the California Mastitis Test (CMT) score. It can be concluded that an average of 16 Etawa crossbred goat tail samples (lactaxes) were found to be 14 goat tail samples that were indicated positive for subclinical mastitis, with a detection rate of 87.5%. Subclinical mastitis is caused by several factors, including poor barn management, the age of the lactation and sow, and poor hygiene during milking, which can lead to contamination of healthy goats from sick PE goats.
Deteksi Resistensi Isolat Bakteri Salmonella sp. dari Feses Segar Ayam Petelur (Gallus gallus domesticus) terhadap Antibiotik Widia Restu; Candra Dwi Atma; Yeti Kurniasih; Kholik Kholik
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 3 (2026): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i3.1199

Abstract

Antibiotic resistance is a global problem that connects human and animal health. This resistance occurs when bacteria acquire genes that allow them to survive when exposed to antibiotics. Wanasaba District, East Lombok is one of the districts that has a large population of laying hens, one of the infectious diseases that often attacks poultry and is treated using antibiotics is salmonella sp. This study aims to detect the resistance of Salmonella sp. bacterial isolates derived from fresh feces of laying hens (Gallus gallus domesticus) to antibiotics. This study is a laboratory experimental study conducted in November 2025 with a total of 3 samples of fresh feces of laying hens taken using a purposive sampling method. Bacterial isolation was carried out using Xylose Lysine Deoxycholate (XLD) agar media, then identified using biochemical tests to confirm the presence of Salmonella sp. Antibiotic resistance tests were carried out using the Kirby-Bauer disc diffusion method using 30 μg tetracycline antibiotics, 10 μg gentamicin, and 5 μg enrofloxacin. The results showed that all Salmonella sp. isolates from the three samples demonstrated 100% sensitivity to the three types of antibiotics tested. These results indicate that Salmonella sp. isolates in laying hens at the study site have not yet developed resistance to the antibiotics used. However, monitoring antibiotic use and implementing good farm management are still necessary to prevent the emergence of bacterial resistance in the future.
Co-Authors Afnani, Daniah Ashri Akhmad Sukri Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alza Hamdi Putra Andreayani Attamimi Andreyani Attamimi Angkasa, Winoto Aprianti, Aurira Thrisma Dwi Ariandoko Ariandoko Asmara, Zinie Hikmah Atma, Candra Dwi Barmawi, Muhammad Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Christin Marganingsih Santosa Dedi Putra Dita Deta Ramdani Qadriyah Dharmawibawa, Iwan Doddy Dina Oktaviana Dina Oktaviana Dina Oktaviana Dina Oktaviana Dina Wurinaharuma Dwipal Tio Ikhsan Hadi Erlin Yustin Tatontos Erni Fadilah, Aulia Rizki Fauzi Saputra Fernando Jose Immanuel Clinton Situmorang Garnika, Eneng Gunarti Gunarti Gunarti, Gunarti Gunawan Gunawan Gunawan Hamzah, Arshq Mirza Humairah, Ira Husnaini, Farhan Imron Rosyadi Ismail Marzuki Iwan Desimal Iwan Doddy Dharmawibawa Jayanti Mandrasari Kadek Rachmawati Karina, Soca Karinda, Kisvan Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Khairullah, Aswin Rafif Kristian Wolagole Kunti Tirtasari Kunti Tirtasari Kurnia Rizki Kurnia, Rofi Lalu Faesal Fajri Lalu Purnama Tasyakusuma Lukitasari, Lina Maratun Janah Maratun Jannah Mardani Mariyam Al Haddar Mashur Mashur Mbura, Yonanda Verawati Haslinda Moch Taufik Hidayatullah Muhammad Hidayat Muhammad Munawaroh Muhammad Munawaroh Muhammad Munawaroh Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Munawaroh, Muhammad Munawer Pradana Ni Ketut Alit Suarti Ningrum, Adek Livia Yunita Nofisulastri, Nofisulastri Nova Kurnia Novarina Sulsia Novarina Sulsia Ista&#039;In Ningytas Novarina Sulsia Ista'In Ningtyas Nur Rusdiana Nurul Khairah Prabowo, Gwenny Ichsan Pradana, Munawer Pratiwi, Dhea Siskha Pudji Srianto Purwanti, Ni Luh Lasmi Qurnianingsih, Ema Rahmawati Rahmawati Rahmawati, Septiyana Eka Rahmawati, Septyana Eka Rahmawati, Siti Irma Ramdani, Fitrah Akbar Rima Nurmayani Riwu, Katty Hedriana Priscilia Riwu, Katty Hendriana Priscilia Riwu, Katty Hendriana Priscillia Rizki, Kurnia Rusyda, Aqila sabila Sabaruddin Satuman Satuman Sa’diyah, Siti Nurus Seli Nurmayani Septyana Eka Rahmawati Siti Khaerunnisa Siti Nurus Sa’diyah Supriadi Supriadi Supriadi Supriadi Supriadi Syafindri syafindri syafindri Tirtasari, Kunti Ummah, Fithriyah Cholifatul Ummah Urip Urip Urip Urip, Urip Widia Restu wijaya, I Gusti Made Arjana Wiwik Handayani Wungu, Citrawati Dyah Kencono Yeti Kurniasih Yeti Kurniasih Yumi Sartika Zakarias, Herdin Vanek