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Hubungan Body Condition Score dengan Service Per Conception pada Induk Sapi Bali di Desa Kilang Kecamatan Montong Gading Kabupaten Lombok Timur Sabaruddin; Kholik; Dina Oktaviana; Maratun Jannah
Jurnal Sains Peternakan Vol 10 No 1 (2022): Jurnal Sains Peternakan
Publisher : Fakultas Peternakan, Universitas PGRI Kanjuruhan Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jsp.v10i1.6579

Abstract

One indicator of success in reproductive efficiency in artificially inseminated (IB) cattle is the Service per Conception (S/C) value, which is widely associated with the body condition score of the cow known as the Body Condition Score (BCS). The purpose of this study was to determine the relationship between value of BCS and S/C of Bali cattle in artificial insemination of Bali cattle. The type of research is an observational longitudinal study, which was conducted from January to April 2021 in the Kilang village Montong Gading district. The number of samples used in this study was 20 Bali cattle with a purposive sampling method on Bali cattle with two lactations. Assessment of Body Condition Score (BCS) is done visually or by palpation on body fat deposits under the skin around the base of the tail, vertebrae and coccyx, while the S/C score is assessed by comparing the number of artificially inseminated (IB) to the occurrence of pregnancy. The relationship between BSC and S/C was carried out using the Pearson Chi-square test. The results showed that the 20 Bali cattle that were sampled had an average Body Condition Score of 2.4 ± 0.680, while the Service per Conception value had an average of 1.55 ± 0.686. The results of the Pearson Chi-square test analysis regarding the relationship between Body Condition Score and Service per Conception in Bali cattle did not have a non-significant relationship because p-value > 0.05 (p-value = 0.08) with = 0.05.
THE RELATIONSHIP BETWEEN GASTROINTESTINAL WORMS PREVALENCE AND AGE OF CIDOMO DRILLING HORSE IN MATARAM CITY syafindri syafindri; kholik kholik; kunti tirtasari; maratun janah
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.30-38

Abstract

Kuda penarik cidomo yang digunakan sebagai alat transportasi di Kota Mataram sangat rentan terhadap penyakit cacingan karena hidup di jalan dengan pemeliharaan secara tradisional yang akan berdampak pada kesehatan kuda. Telah dilakukan penelitian cross-sectional pada kuda penarik cidomo di Kota Mataram pada bulan Februari sampai Maret 2021. Penelitian ini bertujuan untuk mengetahui hubungan prevalensi cacingan saluran cerna dengan umur kuda penarik cidomo di Kota Mataram. Sebanyak 74 feses kuda penarik cidomo dikumpulkan langsung dari dubur kuda untuk pemeriksaan telur cacing guna mengukur prevalensi cacingan saluran cerna. Pemeriksaan telur cacing pada feses dilakukan di Laboratorium Equine Clinical Skill Center Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika dengan metode sedimentasi dan flotasi. Hubungan prevalensi cacingan saluran cerna dengan umur kuda penarik cidomo dianalisis dengan uji Pearson dengan α = 0,05. Hasil penelitian menunjukkan bahwa prevalensi cabut cidomo yang terinfeksi cacing adalah 76,1% (53/7). Telur cacing yang teridentifikasi adalahStrongylus spp, Trichostrongylus spp, Cyathostoma spp, Ostertagia spp, dan Parascaris equorum . Hubungan prevalensi infeksi cacing saluran cerna dengan umur kuda penarik cidomo tidak bermakna dengan p = 0,22.
PHENOTYPIC DETECTION OF Escherichia coli PRODUCING Extended Spectrum Beta Lactamases (ESBLs) IN THE REPRODUCTIVE TRACT BALI COW Lalu Purnama Tasyakusuma; kholik kholik; maratun janah; alfiana laili dwi agustin; septyana eka rahmawati
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.64-73

Abstract

Escherichia coli bacteria that are resistant to β-lactam antibiotics and may be able to produce Extended-spectrum β-lactamase (ESBLs) have been found in the reproductive tract of Bali cattle. Escherichia coli that produce ESBL will cause antimicrobial resistance (AMR) which has become a global health problem. The purpose of this study was to detect phenotypically the presence of Escherichia coli that produces ESBL from the reproductive tract of Bali cattle. This research was conducted in February 2022 in Lando Village, Terara District, East Lombok Regency using 8 female Bali cattle on smallholder farms. Reproductive fluid samples were collected using a plastic sheet gun which was inserted into the Brain Infusion Heart (BHI) media and incubated at the Public Health and Calibration Laboratory of West Nusa Tenggara Province. The samples were then cultured in EMBA media for the isolation of Escherichia coli. The isolated Escherichia coli were then identified by gram staining and biochemical tests based on Bergey's manual of determinative bacteriology. The identified Escherichia coli were screened using the disc diffusion method using the antibiotics Penicillin G10U, Ceftazidime 30 μg, and Cefotaxime 30 μg followed by a confirmation test using the double disk synergy test (DDST) method. The results of the screening test showed that only 12.5% of Escherichia coli (1 out of 8 samples) were resistant to Penicillin G, but they had not been detected to produce ESBL phenotypically.
POTENTIAL OF SEAWEED (Eucheuma cottonii) EXTRACT AS A HEPATOPROTECTOR IN RABBIT (Oryctolagus cuniculus) INDUCED ORGANOPHOSPHATE PESTICIDES Alza Hamdi Putra; kholik kholik; novarina sulsia ista'in ningtyas
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.21-29

Abstract

The use of organophosphate pesticides in developing countries is already widespread and can contaminate animal feed or human food ingredients which has an impact on liver damage and other organs. The use of seaweed containing polyphenols as a hepatoprotector for organophosphate poisoning has not been widely used by the public. The purpose of this study was to determine the potential of seaweed extract as a hepatoprotector in organophosphate-induced rabbits as seen from serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) levels. This study used a complete randomized design with 20 male rabbits as experimental animals which were divided into 4 treatments with 5 repetitions, namely: P0 was the control group which was only given distilled water, P1 was the group given seaweed extract at a dose of 200 mg/Kg BW, P2 is the group given seaweed extract at a dose of 400 mg/KgBB and P3 is the positive control group which is not given seaweed extract and distilled water. On the 15th day the P1, P2, and P3 groups were given profenofos pesticide at a dose of 50 mg/kg BW. The results showed a very significant difference (p-value = 0.02, α = 0.05) in SGOT levels between the P0 control group (135.4 ± 67.00 U/L) and the P1 treatment group (41.8 ± 17.45 U/L), P2 (25.8 ± 8.75 U/L), and the positive control group P3 (70 ± 29.04). The results of the study also found that there was no significant difference in SGPT levels in the P0 control group and the P1, P2, and P3 control groups. Giving seaweed extract with different doses provides a hepatoprotector effect by reducing the release of SGOT into the blood.
DETECTION OF ANTIBIOTIC RESISTANT IN Escherichia coli FROM THE REPRODUCTIVE TRACT OF BALI CATTLE ON SMALLHOLDER FARM Kholik Kholik
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.44-53

Abstract

Escherichia coli originating from animals, humans, and the environment can spread antibiotic-resistant genes and can encourage antimicrobial resistance (AMR) which is a global health problem. The purpose of this study was to detect the presence of Escherichia coli which is resistant to several antibiotics from the reproductive tract of Bali cattle on smallholder farms on the island of Lombok. This research is a cross-sectional study conducted from March to June 2021 using 8 female Bali cattle that experience reproductive disorders on community farms in Lando Village, East Lombok Regency to collect their reproductive tract fluids using an artificial insemination plastic sheet gun. Reproductive fluid samples are placed on BHIB (Brain Heart Infusion Broth) medium. Escherichia coli cultures were carried out on Eosin Methylene Blue Agar (EMBA) and identified by Gram staining and biochemical tests conducted at the Public Health and Calibration Laboratory, West Nusa Tenggara Province. Antibiotic sensitivity test on isolated Escherichia coli was carried out by disc diffusion method using 5 antibiotics including Penicillin G 10U, Oxytetracycline 30 µg, Gentamicin 10 µg, Tetracycline 30 µg, and Cefotaxime 30 µg. The results showed that 2 (two) 25% Escherichia coli bacteria were successfully cultured on EMBA media and isolated from 8 samples of the reproductive fluids of Bali cattle that were collected. The results of the Escherichia coli sensitivity test to antibiotics found that Escherichia coli samples were 100% resistant to Penicillin G, 100% resistant to Oxytetracycline, 100% resistant to Gentamicin, and 50% resistant to Tetracycline, and 100% resistant to Cefotaxime from 2 isolated Escherichia coli. This explains that Bali cattle in community farms have the potential to spread Escherichia coli which has an impact on the emergence of AMR.
Detection Of Antibiotic Residues In Quail Eggs At The Traditional Market Of Mataram City Nurul Khairah; Kholik Kholik
Mandalika Veterinary Journal Vol 2, No 2 (2022): OKTOBER
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.894 KB) | DOI: 10.33394/mvj.v2i2.6223

Abstract

Quail farms (Coturnix sp.) are inseparable from the antibiotic uses, breeders who use antibiotics without observing the appropriate rules could cause residues in the tissues and organs of animals, including in eggs. Consumption of quail eggs containing by antibiotic residues could have a negative impact on health. Quail eggs consumed by the public generally come from traditional markets. The survey results show that the Karang jasi market is a traditional market in Mataram city that sells the most quail eggs. This study aimed to detect antibiotic residues in quail eggs in the traditional market of Mataram city. This research was a descriptive study, used the survey method with a cross-sectional approach. 18 Samples were taken from the traditional market of Mataram city which is sells the most quail eggs. The examination of samples was done qualitatively using a screening test by bioassay. The test results showed that all samples tested were negative from antibiotic residue. Based on the result, could be councluded that the quail eggs in the karang jasi traditional market are safe for consumption.
Prevalensi dan Derajat Infeksi Helminthiasis Gastrointestinal pada Sapi Bali (Bos sondaicus) di Peternakan Rakyat Mutu Desa Sepayung Kecamatan Plampang Kabupaten Sumbawa Besar Nusa Tenggara Barat Ariandoko Ariandoko; Kholik Kholik; Candra Dwi Atma; Novarina Sulsia Ista'In Ningytas
Mandalika Veterinary Journal Vol 1, No 1 (2021): April
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.609 KB) | DOI: 10.33394/mvj.v1i1.3605

Abstract

Bali cattle is one of the cattle that are more in demand by the community, especially the people ofSumbawa Besar. Healthy cows that are in a state or physiological body functioning normally, which meansthat cattle avoid various diseases, especially Helminthiasis. The purpose of this study was to determine theprevalence and degree of gastrointestinal helminthiasis infection in Balinese cattle (Bos sondaicus) in MutuPeople's Farms in Sepayung village, Plampang sub-district, Sumbawa Besar district, NTB. This research is adescriptive study with Based on Rates or calculation of the prevalence rate and degree of GastrointestinalHelminthiasis infection in bali cattle feces at Mutu People's Farm in Sepayung Village, Plampang District,Sumbawa Besar Regency, West Nusa Tenggara. This research was conducted in February 2020 in theLaboratory of the Faculty of Veterinary Medicine, West Nusa Tenggara University, examined using the McMaster method and counted the number of worm eggs per gram of feces. Based on the results of laboratorytests conducted on 37 bali cattle feces samples, found 6 samples infected with Trichostrongylus sp. and 2samples were infected with Ostertagia sp. The results of examinations that have been done, the prevalencefound in this study is Trychostrongylus 16.21% while in Ostertagia it is 5.40% with the degree of infection inTrichostrongylus moderate infections and in mild infections Ostertagia. Based on the results of laboratorytests conducted on 37 bali cattle feces samples, found 6 samples infected with Trichostrongylus sp. and 2samples were infected with Ostertagia sp. The results of examinations that have been done, the prevalencefound in this study is Trychostrongylus 16.21% while in Ostertagia it is 5.40% with the degree of infection inTrichostrongylus moderate infections and in mild infections Ostertagia. Based on the results of laboratorytests conducted on 37 bali cattle feces samples, found 6 samples infected with Trichostrongylus sp. and 2samples were infected with Ostertagia sp. The results of examinations that have been done, the prevalencefound in this study is Trychostrongylus 16.21% while in Ostertagia it is 5.40% with the degree of infection inTrichostrongylus moderate infections and in mild infections Ostertagia.
Profil Uji Biokima Hasil Isolasi Escherichia coli pada Feses, Air Minum Dan Air Saluran Buangan Kandang Sapi Bali Di Kelompok Tani Ternak Menemeng (KT2M) Kabupaten Lombok Tengah Gunawan Gunawan; Kholik Kholik; Alfiana Laili Dwi Agustin
Mandalika Veterinary Journal Vol 2, No 1 (2022): April
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.611 KB) | DOI: 10.33394/mvj.v2i1.5152

Abstract

Escherichia coli termasuk family entero bakteriae yang hidup secara komensial di saluran pencernaan hewan maupun manusia. Di Negara tropis Escherichia coli digunakan sebagai indicator kontaminasi air yang berasal dari feses. Peternakan rakyat seperti KT2M dekat sekali dengan pemukiman rakyat. Sampai saat ini belum ada profil biokimia di feses, air minum dan saluran buangan (WHO, 1995). Tujuan dari penelitian ini untuk mengetahui Biokimia hasil isolasi Escherichia coli pada feses sapi Bali. Penelitian ini menggunakan metode penelitian deskriptif dan bersifat eksploratori (eksploratif) Laboratorium. Dari 18 sampel isolasi Escherichia coli dari feses, 1 dari air minum dan 1 dari air buangan yang positif untuk melihat profil Biokimia Eschericia coli pada feses sapi Bali. Hasil penelitian Uji Biokimia dari 18 sampel feses, 1 dari air minum dan 1 dari air saluran buangan didapatkan Indol (+), Voges Proskauer (-), Methyl Red (+), dan Cimon Citrate (-), Glukosa (+), Sukrosa (+), Alkali Fosfat (+), TSIA (A/A Gas +), dan Urea (-). Dari hasil penelitian Uji Biokimia yang di ambil dari isolat Escherichia coli pada feses, air minum dan air saluran buangan kandang di Kelompok Tani Ternak Menemeng (KT2M) bahwa positif Escherichia coli. Perlunya perhatian dari Kelompok Tani Ternak Menemeng (KT2M) terhadap air minum yang diberikan dan perlunya sanitasi kandang secara optimal serta air saluran buangan agar diperhatikan agar tidak berdekatan dengan sumber air minum.
Distribusi Cacing Trematoda Saluran Pencernaan Katak Dari Berbabgai Lokasi Persawahan Di Kabupaten Lombok Timur Kristian Wolagole; Kholik Kholik; Supriadi Supriadi; Dina Oktaviana
Mandalika Veterinary Journal Vol 1, No 1 (2021): April
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.423 KB) | DOI: 10.33394/mvj.v1i1.3615

Abstract

Trematode worms have been found in various types of frogs which cause disease in frogs or toads can be a reservoir for these worms. Lombok Island has many rice fields which are habitat for frogs. Frogs that live in this environment allow direct contact with various types of Trematodes. The purpose of this study was to determine the type and distribution of Trematoda worms found in the digestive tract of frogs in the rice fields of East Lombok Regency. A cross-sectional survey study using purposive sampling methods on frogs in three rice fields in East Lombok Regency was carried out in February 2020. Worms were collected from the digestive tract of frogs and fixed with 70% warm alcohol, cleaned with alcohol, and examined under a microscope. A total of 64 frog samples were taken in three rice fields, East Lombok Regency. A sample examination was carried out  at the Equin Clinical Center Skill laboratory Faculty of Veterinary Medicine of Mandalika University of Education. The results showed that Mesocoelium spp was distributed among frogs in three rice fields in East Lombok Regency, with a prevalence of 45% in Pringgabaya Village, 50% in Suele Village, and 53.57% in Tanjung Teros Village.
Mapping Chemical Hazards in Animal Food Origin Product for Food Safety Teaching Materials Nova Kurnia; Kholik Kholik; Khaeruman Khaeruman
Jurnal Penelitian Pendidikan IPA Vol 9 No 1 (2023): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i1.2534

Abstract

Animal origin food product is quite vulnerable to the threat of chemical hazards. Mapping potential chemical hazards is needed to establish good and safe food handling methods. In addition, the mapping can be used as the basis for the preparation of food safety teaching materials that have not been available so far. The purpose of this study is to map various potential chemical hazards in food of animal origin food product to be used as teaching materials for food safety. This research is included as a literature study by collecting various types of scientific references that outline potential chemical hazards that can threaten animal origin food product. The results showed potential chemical hazards in the form of antibiotic residues in beef, chicken, and eggs; excess nitrates and nitrites in beef; scombrotoxin in cob fish; pesticides and heavy metals in honey; and formalin and borax in chicken meat and fish. The results of mapping the potential chemical hazards of animal origin food product can then be used to compile food safety teaching materials
Co-Authors Afnani, Daniah Ashri Akhmad Sukri Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alza Hamdi Putra Andreayani Attamimi Andreyani Attamimi Aprianti, Aurira Thrisma Dwi Ariandoko Ariandoko Asmara, Zinie Hikmah Atma, Candra Dwi Barmawi, Muhammad Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Candra Dwi Atma Dedi Putra Dita Deta Ramdani Qadriyah Dharmawibawa, Iwan Doddy Dina Oktaviana Dina Oktaviana Dina Oktaviana Dina Wurinaharuma Dwipal Tio Ikhsan Hadi Erlin Yustin Tatontos Erni Fadilah, Aulia Rizki Fauzi Saputra Fernando Jose Immanuel Clinton Situmorang Garnika, Eneng Gunarti Gunarti Gunarti, Gunarti Gunawan Gunawan Gunawan Hamzah, Arshq Mirza Humairah, Ira Husnaini, Farhan Ismail Marzuki Iwan Desimal Iwan Doddy Dharmawibawa Jayanti Mandrasari Kadek Rachmawati Karina, Soca Karinda, Kisvan Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Katty Hendriana Priscilia Riwu Khairullah, Aswin Rafif Kristian Wolagole Kunti Tirtasari Kunti Tirtasari Kurnia Rizki Kurnia, Rofi Kurniawan, Shendy Canadya Lalu Faesal Fajri Lalu Purnama Tasyakusuma Lukitasari, Lina Maratun Janah Maratun Janah, Maratun Maratun Jannah Mariyam Al Haddar Mashur Mashur Mbura, Yonanda Verawati Haslinda Moch Taufik Hidayatullah Muhammad Hidayat Muhammad Munawaroh Muhammad Munawaroh Muhammad Munawaroh Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Muhammad Rama Imam Saputra Mujmal, Khaerul Munawaroh, Muhammad Munawer Pradana Ni Ketut Alit Suarti Ningrum, Adek Livia Yunita Nofisulastri, Nofisulastri Nova Kurnia Novarina Sulsia Novarina Sulsia Ista'In Ningytas Novarina Sulsia Ista'In Ningtyas Nur Rusdiana Nurul Khairah Prabowo, Gwenny Ichsan Pradana, Munawer Pratiwi, Dhea Siskha Pudji Srianto Purwanti, Ni Luh Lasmi Qurnianingsih, Ema Rahmawati Rahmawati Rahmawati, Septiyana Eka Rahmawati, Septyana Eka Rahmawati, Siti Irma Ramdani, Fitrah Akbar Rima Nurmayani Riwu, Katty Hedriana Priscilia Riwu, Katty Hendriana Priscilia Riwu, Katty Hendriana Priscillia Rizki, Kurnia Rusyda, Aqila sabila Sabaruddin Safitri, Indi Yulia Satuman Satuman Sa’diyah, Siti Nurus Seli Nurmayani Septyana Eka Rahmawati Siti Khaerunnisa Siti Nurus Sa’diyah Supriadi Supriadi Supriadi Supriadi Supriadi Syafindri syafindri syafindri Tirtasari, Kunti Ummah, Fithriyah Cholifatul Ummah Urip Urip Urip Urip, Urip wijaya, I Gusti Made Arjana Wiwik Handayani Wungu, Citrawati Dyah Kencono Yeti Kurniasih Yumi Sartika Zakarias, Herdin Vanek