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Journal : Journal of Tropical Crop Science

Cloning and Characterization of Partial Chlorophyll a Oxygenase (CAO) Gene Involved in Soybean Shade Tolerance Mechanism Khumaida, Nurul; Kisman, Kisman; Sopandie, Didy
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2936.921 KB) | DOI: 10.29244/jtcs.2.2.1-4

Abstract

Plant tolerance mechanism under shade stress is indicated by high chlorophyll-b content, which is synthesized from chlorophyll a by chlorophyll a-oxygenase (CAO) gene. The study was aimed to clone and characterize the partial of CAO gene involved in shade tolerance mechanism in soybean.  Total RNA was isolated from the second trifoliate leaves of soybean plants and the first strand cDNA was prepared using Reverse Transcriptase Moloney Murine Leukemia Virus (RT-M-MLV) (RNase H-). Northern blot hybridization was used to analyze expression of partial CAO gene. The result showed that one partial gene, CAO 3-4 (1,052 bp), comprised of 292 adenine (a), 241 cytosine (c), 276 guanine (g) and 242 thymine (t), therefore the number of amino acid deduction was 338.  The expression of CAO 3-4 partial gene was higher in shade tolerant genotypes than in shade sensitive genotypes, both under 50% shade and dark. These results demonstrated that the CAO gene is involved in shade tolerance mechanism of soybean.
Biodiversity Assessment of Foxtail Millet (Setaria italica L.) Genotypes Based on RAPD Marker ARDIE, Sintho Wahyuning; Khumaida, Nurul; Fauziah, Nurul; Yudiansyah, Yudiansyah
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1155.767 KB) | DOI: 10.29244/jtcs.4.1.21-25

Abstract

Foxtail millet (Setaria italica L.) is an important crop in areas where harsh environmental conditions limit crop productivity, including in high salinity and drought prone areas. In Indonesia millet is cultivated in certain areas; however, superior varieties are less developed in the country. The objective of this study was to analyze the genetic diversity among foxtail genotypes using RAPD markers. Genomic DNA of ten foxtail millet genotypes was amplified using 26 random primers through RAPD analysis. Of these primers, 22 produced reproducible amplicons and were polymorphic among the 10 foxtail millet genotypes. The number of polymorphic markers for each primer varied from 1 (primer E15) to 14 (primer M17). The amplified product size ranged from 120 to 2500 base pairs (bp). A dendrogram constructed based on the UPGMA clustering method put all genotypes in 5 distinct groups at 0.64 coefficient level. Diverse genotypes identified in this study can be used as potential parents in an efficient crop improvement program.
Nonparametric Stability Analysis of Starch Content of Gamma Irradiated Cassava at Three Locations in West Java, Indonesia Agustina, Ferra Anggita; Khumaida, Nurul; Ardie, Sintho Wahyuning; Syukur, Muhamad
Journal of Tropical Crop Science Vol 7 No 02 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.771 KB) | DOI: 10.29244/jtcs.7.02.66-74

Abstract

Cassava is one of the largest starch producing tuber crops in Indonesia. Tapioca from cassava starch can be used as a raw materials for a variety of foods including biscuits, instant porridge, meatball, sausage, nuggets, and flour condiment. Breeding programs to improve cassava yield and starch content by using mutagen gamma rays irradiation was tested in four cassava generations (M1V4). Cassava is propagated by stem cuttings and the new characteristic obtained from gamma irradiation mutation is stable and can be passed from one generation to the next. Cassava mutants were obtained by evaluating the performance in different environmental conditions. The testing of yield adaptability and stability through a series of multi location is an important step before a new variety can be released. The aim of this research was to compare nonparametric stability and to evaluate the stability of the starch content of 16 genotypes (14 mutants and 2 varieties) in three locations in West Java Province i.e. Tapos (Depok), Cikarawang (Bogor), and Ciseeng (Bogor). Experiments were conducted in a completely randomized block design with three replications nested in each environment. Testing of yield stability in this study used four approaches with 10 parameters of nonparametric method. Starch content was estimated using gravimetric method. Based on frequency stability ranking of starch content from gravimetric method the G63142 genotype had the highest starch content estimation (29.99%), and the top five genotypes with high starch content were G63142, G61142, “Manggu”, G62151, and G63124. G61142 was categorized as genotype with static and dynamic stability, therefore this genotype is a very potential mutant to be released, whereas G63124 is categorized as a genotype with a static stability. Genotypes G63142, G62151, and “Manggu” had variable but relatively high starch content.
Co-Authors , Kisman , Kisman , Sukendah , Witjaksono . Wusnah A. Sofwan Achmad . Adam Saepudin Adi Setiadi Agus Purwito Agustina, Ferra Anggita AHMAD JUNAEDI Ahmad Rifqi Fauzi Ali Djamhuri Almia Permata Putri Amril Aman and Gustaaf Adolf Wattimena Andi Dahliaty Andina Fabrini Firdausya Aniversari Apriana Aniversari Apriana Aniversari Apriana Ardana, I. N. Kutha Ariny Jihan Thifany Aris Purwanto Arrin Rosmala Atmitri Sisharmini Atmitri Sisharmini Atmitri Sisharmini Atmitri Sisharmini Bambang S. Purwoko Bambang Sapta Purwoko Bhayu Hartanti Buang Abdullah Cakranegara, Pandu Adi Candra Catur Nugroho Cucu Sukmana, Cucu dan Kurniawan Rudi Trijatmiko Danar Dono Dede J Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede Jajat Sudrajat Dede Kurniadi DEWI SARTIAMI Dewi Sukma Dewi Sukma Didy Sopandie Diny Dinarti Dwi Pratiwi Edi Santosa Efendi, Darda Emmy Darmawati Enny Sudarmonowati Evi Fitriany Faqih Udin Freddy Tua Musa Panggabean Gustaaf A Wattimena, Gustaaf A Gustaaf A. Wattimena Gustaaf Adolf Wattimena Gustaaf Adolf Wattimena HADIPOENTYANTI, ENDANG Hartati, RR. Sri Heldiyanti, Rina Hendri Hermawan Adinugraha HUSNI, ALI I Ismayani I MADE ARTIKA Ida Duma Riris Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Roostika Ika Roostika Indrastuti Apri Rumanti Irdika Mansur Irfan MARTIANSYAH Iskandar Z Siregar Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Isnani Subekti Iswari S Dewi Iswari S. Dewi Iswari Saraswati Dewi Jalaluddin Jalaluddin Kartika Ning Tyas Kartika Ning Tyas Kartika Ning Tyas Kasutjianingati . Kisman Kisman Kurniawan R. Trijatmiko Kurniawan Rudi Trijatmiko Kurniawan Rudi Trijatmiko La Muhuria Lefin Kafindra Leni Siswati Lestari, Risza Putri Lisnawaty Simatupang Lola Adres Yanti Lukman Hakim M Murtadha M. Amir M. Asyabul Zikki Maemonah, Maemonah MARIA BINTANG Marini Damanik Mariska, Ika Maryati Evivani Doloksaribu MEYNARTI SARI DEWI IBRAHIM MEYNARTI SARI DEWI IBRAHIM Muaz Adbdul Karim Muaz Adbdul Karim Muhamad Syukur Muhammad Irfan Habibi MUHAMMAD SYUKUR Mulyatno, Mulyatno Munif Ghulamahdi N. Sri i Hartati Nafilawati wa ode Nata Suharta Natalini Nova Kristina Natalini Nova Kristina Nataniel Tandirogang Nura , Nurbaiti Nurbaiti Nurhasybi Nurhasybi Nurul Fatimah Nurul Fauziah O K Sofyan OTIH ROSTIANA OTIH ROSTIANA Pienyani Rosawanti Rahmi Henda Yani Rasyad, M. Ashadi Reny Herawati Rina Hapsari Wening Rinda Cahyana Riyanti Catrina H S Riza Arief PUTRANTO Rizkiannur Putri, Amalia Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rozanna Dewi Ryan Budi Setiawan Sadewi Maharani Sadewi Maharani Safitri Safitri Saronom S ilaban Sartika Widowati Sarwono Hardjowigeno Shafwan Fahmi Silawati, Tutisiana Silawati Tutisiana Sintho Wahyuning Ardie Siti Aisyah Siti Kurniawati Slamet Budijanto Slamet Susanto Sobir Sobir Sri Enny Triwidiastuti Sri Helianty Sry Rahmadani Sudarsono Sudarsono Sudarsono Suhesti, Sri Sukma, Dewi Sulastri Sulastri Sulhatun Sulhatun SUPRIADI SUPRIADI Suryati Suryati Suryo Wiyono Sutalaksana, Iftikar Z Sutrisno Mardjan Sutrisno, Sutrisno Suyanti Kasimin Syamsul Bahri Syamsul Bahri Tarigan, Asmara Iriani Tengku Mia Rahmiati Teti Arabia Tri Handayani Tri Joko Santoso Tri Lestari Mardiningsih Tri Lestari Mardiningsih, Tri Lestari Trikoesoemaningtyas Trismi Ristyowati Ulfah J. Siregar Ulfah Juniarti Siregar Ummu Kalsum Waizul Fahri Purba Waras Nurcholis Warid Warid WATTIMENA, G. A. Wening Enggarini Wening Enggarini Widiatmaka Widodo Widodo Widya Hartati, Widya Wijayanti, Mustika Eka Willy Bayuardi Suwarno Yudiansyah Yudiansyah Yulia, Endah Yulia Yundari, Yundari Yunita, Rossa Zuli Nuraeni