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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Seminar Nasional Aplikasi Teknologi Informasi (SNATI) Agrotrop : Journal on Agriculture Science Jurnal Agrista JIK Jurnal Ilmu Komputer BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Keteknikan Pertanian Cropsaver : Journal of Plant Protection Jurnal Algoritma LISANIA Jurnal Ilmu dan Pendidikan Bahasa Arab Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains OPSI AGRIVITA, Journal of Agricultural Science Buletin Kebun Raya agriTECH JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Kebijakan dan Pelayanan Publik Law and Justice Jurnal Matematika Sains dan Teknologi Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Teknik Informatika STMIK Antar Bangsa Jurnal Ergonomi dan K3 Jurnal Agrotek Tropika Jurnal Pengabdian Masyarakat AbdiMas JURNAL PERTANIAN PRESISI (JOURNAL OF PRECISION AGRICULTURE) Journal of Tropical Crop Science Jurnal Ilmiah Pendidikan Scholastic JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan WIDYA LAKSANA Ensiklopedia of Journal Molekul: Jurnal Ilmiah Kimia Menara Perkebunan Jurnal Bidan Cerdas Jurnal Sains dan Teknologi Reaksi Jurnal Kebidanan Mutiara Mahakam Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Jurnal Sains Agro Jurnal ABDIKU Indian Journal of Forensic Medicine & Toxicology Indonesian Science Education Research (ISER) Jurnal Konstitusi Jurnal Teknologi Kimia Unimal Jurnal Algoritma Jurnal Penelitian Kehutanan Wallacea
Nurul Khumaida
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia
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Journal : Jurnal AgroBiogen

 1 
Delivering of Over-Expression Construct OsWRKY76 Candidate Gene in Rice cv. Nipponbare through Agrobacterium tumefaciens Apriana, Aniversari; Sisharmini, Atmitri; Enggarini, Wening; Sudarsono, Sudarsono; Khumaida, Nurul; Trijatmiko, Kurniawan R.
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
Publisher : Jurnal AgroBiogen

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Abstract

Plant genetic improvement can be done through classical breeding or genetic engineering. WRKY is a transcription factor involved in regulating plant defense responses. OsWRKY76 gene is located in a narrow segment of chromosome 9 which is identified previously to be related to wide spectrum resistance in rice. A sequence of OsWRKY76 (+1.200 bp) has available in the gene bank and it makes possible to isolate, clone, and construct the gene into over-expression vector. The aim of this research was to assemble an over-expression construct of OsWRKY76 candidate gene and introduce it into rice through Agrobacterium-mediated transformation. A construct of pCAMBIA-1301::35S::OsWRKY76 has been successfully assembled and transformed into embryogenic calli of rice cv. Nipponbare using A. tumefaciens strain Agl-1 and EHA 105. A number of 126 independent lines has been produced, in which Agl-1 showed 3.8 times more efficient than EHA 105. PCR analysis of randomly selected 25 independent lines showed that all of them positively contained hptII gene, a selectable marker used in the over-expression construct of the OsWRKY76 candidate gene. Based on the result, it could be concluded that the over-expression construct of OsWRKY76 candidate gene have been successfully introduced into the tissue of Nipponbare.
Indirect Organogenesis and Somatic Embryogenesis of Pineapple Induced by Dichlorophenoxy Acetic Acid Roostika, Ika; Mariska, Ika; Khumaida, Nurul; Wattimena, Gustaaf A
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

ABSTRACTIndirect Organogenesis and Somatic Embryogenesis ofPineapple Induced by Dichlorophenoxy Acetic Acid. IkaRoostika, Ika Mariska, Nurul Khumaida, and Gustaaf A.Wattimena. This research aimed to study the effect of 2,4-D,AdS, and basal media to the regeneration of pineapplethrough indirect organogenesis and somatic embryogenesis,and to study the complete event of somatic embryogenesis.Callus formation was induced by 21, 41, and 62 μM 2,4-Dwith addition of 9 μM TDZ. The non embryogenic calli weretransferred onto 4.65 μM Kn containing medium.Embryogenic callus formation was induced on MS or Bacbasal media consisted of N-organic compounds withaddition of AdS (0, 0.05 and 0.1 μM). The embryogenic calliwere regenerated on modified MS medium with addition of0.9 μM IBA, 1.1 μM BA, 0.09 μM GA3 or MS mediumsupplemented with 0.018 mM BA. The result proved that thesingle auxin of 2,4-D was not enough to induce embryogeniccells. Therefore the non embryogenic calli were regeneratedthrough organogenesis. The 21 μM 2,4-D yielded high level ofcallus formation (80%), higher fresh weight (0.2 g/explant)and higher number of shoot (25 shoots/explant in twomonths). Embryogenic calli were produced on N-organiccompounds enriched media. The regeneration mediumsignificantly affected the level of browning, where the MSmedium with addition of 0.018 mM BA yielded lower level ofbrowning. There was an interaction of embryogenic callusinduction medium and regeneration medium to the numberof mature somatic embryos. The embryogenic callusinduction on MS medium enriched with N-organiccompounds and 0.05 μM AdS followed by the regenerationof somatic embryos on MS medium with addition of 0.018mM BA was the best treatment which yielded 17 maturesomatic embryos/explant
Introduksi Konstruk Over-Ekspresi Kandidat Gen OsWRKY76 melalui Agrobacterium tumefaciens pada Tanaman Padi Nipponbare Aniversari Apriana; Atmitri Sisharmini; Wening Enggarini; Sudarsono Sudarsono; Nurul Khumaida; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p19-27

Abstract

Delivering of Over-Expression Construct OsWRKY76Candidate Gene in Rice cv. Nipponbare throughAgrobacterium tumefaciens. Aniversari Apriana, AtmitriSisharmini, Wening Enggarini, Sudarsono, Nurul.Khumaida, and Kurniawan R. Trijatmiko. Plant geneticimprovement can be done through classical breeding orgenetic engineering. WRKY is a transcription factor involvedin regulating plant defense responses. OsWRKY76 gene islocated in a narrow segment of chromosome 9 which isidentified previously to be related to wide spectrumresistance in rice. A sequence of OsWRKY76 (+1.200 bp)has available in the gene bank and it makes possible toisolate, clone, and construct the gene into over-expressionvector. The aim of this research was to assemble an overexpressionconstruct of OsWRKY76 candidate gene andintroduce it into rice through Agrobacterium-mediatedtransformation. A construct of pCAMBIA-1301::35S::OsWRKY76 has been successfully assembled andtransformed into embryogenic calli of rice cv. Nipponbareusing A. tumefaciens strain Agl-1 and EHA 105. A number of126 independent lines has been produced, in which Agl-1showed 3.8 times more efficient than EHA 105. PCR analysisof randomly selected 25 independent lines showed that allof them positively contained hptII gene, a selectable markerused in the over-expression construct of the OsWRKY76candidate gene. Based on the result, it could be concludedthat the over-expression construct of OsWRKY76 candidategene have been successfully introduced into the tissue ofNipponbare.
Pengaruh Iradiasi Sinar Gama terhadap Pertumbuhan dan Regenerasi Kalus Padi Varietas Ciherang dan Inpari 13 Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Jurnal AgroBiogen Vol 10, No 3 (2014): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n3.2014.p101-108

Abstract

Effect of gamma irradiation on rice callus dependson the irradiation dose used. Irradiation dose is one of thefactors that affect the genetic changes in the cells of plants.High doses can result in tissue death, meanwhile low doseswill result in abnormal changes in plant phenotype. The levelof sensitivity of a plant tissue against gamma irradiation isdifferent. This study aimed to evaluate the growth andregeneration of callus in various irradiation doses of gammaray. The plant materials used were mature embryos of ricevar. Ciherang and Inpari 13. This study used a completelyrandomized design with gamma irradiation treatment atdoses of 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Gy. Eachtreatment consisted of 10 replicates with 5 embryogenic calliper bottle. The results showed that the increasing doses ofgamma irradiation increased the percentage of dead callus,inhibited callus growth, and its ability to regenerate. Thehigh percentages of callus of Ciherang and Inpari 13 thatformed green spots and adventitious shoots were mostlyobtained from controls. The percentages decreased atirradiation doses of 10, 20, 30, and 40 Gy. Moreover, the calliof Ciherang and Inpari 13 were not able to form adventitiousshoots at irradiation doses higher than 40 Gy and 30 Gy,respectively.
Isolation and Homology Analysis of Alanine Aminotransferase Gene of Barley, Foxtail Millet, Cucumber, and Tomato Atmitri Sisharmini; Aniversari Apriana; Tri Joko Santoso; Bambang Sapta Purwoko; Nurul Khumaida; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 16, No 2 (2020): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n2.2020.p59-70

Abstract

Overexpression of alanine aminotransferase (AlaAT) gene can improve nitrogen use efficiency (NUE) in plants. The previous isolated AlaAT genes cannot be freely applied to generate NUE plants due to IPR restriction. Therefore, isolation of the gene from targeted monocot and dicot plants is necessary. The objectives of this study were to isolate AlaAT genes from barley, foxtail millet, cucumber, and tomato and analyze their homology to other isolated AlaAT genes in sequence databases (gene bank). Total RNA was isolated from roots of barley, foxtail millet, cucumber, and tomato, and then converted into cDNA using reverse transcription method. The cDNA was then cloned into pGEM®-T Easy plasmid and the verified clones were sequenced. The amino acid sequences were analyzed for their homologies using Clustal Omega software and phylogenetic tree was constructed. The results showed that the amino acids of AlaAT gene from barley was different from AlaAT genes of tomato and cucumber with homology level less than 80%. Phylogenetic tree predicted that AlaAT genes clustered into three groups with AlaAT genes of foxtail millet and barley clustered in one group together with other monocots in group I. AlaAT genes derived from dicots clustered into two groups in which AlaAT gene of tomato clustered in group II, while that of cucumber was in group III. The identity differences of AlaAT gene of tomato and that of cucumber as well as the estimates of the same enzymatic functions can open up enormous opportunities in genetic engineering research for the development of NUE rice.
Indirect Organogenesis and Somatic Embryogenesis of Pineapple Induced by Dichlorophenoxy Acetic Acid Ika Roostika; Ika Mariska; Nurul Khumaida; Gustaaf A. Wattimena
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n1.2012.p8-18

Abstract

This research aimed to study the effect of 2,4-D,AdS, and basal media to the regeneration of pineapplethrough indirect organogenesis and somatic embryogenesis,and to study the complete event of somatic embryogenesis.Callus formation was induced by 21, 41, and 62 μM 2,4-Dwith addition of 9 μM TDZ. The non embryogenic calli weretransferred onto 4.65 μM Kn containing medium.Embryogenic callus formation was induced on MS or Bacbasal media consisted of N-organic compounds withaddition of AdS (0, 0.05 and 0.1 μM). The embryogenic calliwere regenerated on modified MS medium with addition of0.9 μM IBA, 1.1 μM BA, 0.09 μM GA3 or MS mediumsupplemented with 0.018 mM BA. The result proved that thesingle auxin of 2,4-D was not enough to induce embryogeniccells. Therefore the non embryogenic calli were regeneratedthrough organogenesis. The 21 μM 2,4-D yielded high level ofcallus formation (80%), higher fresh weight (0.2 g/explant)and higher number of shoot (25 shoots/explant in twomonths). Embryogenic calli were produced on N-organiccompounds enriched media. The regeneration mediumsignificantly affected the level of browning, where the MSmedium with addition of 0.018 mM BA yielded lower level ofbrowning. There was an interaction of embryogenic callusinduction medium and regeneration medium to the numberof mature somatic embryos. The embryogenic callusinduction on MS medium enriched with N-organiccompounds and 0.05 μM AdS followed by the regenerationof somatic embryos on MS medium with addition of 0.018mM BA was the best treatment which yielded 17 maturesomatic embryos/explant.
Optimasi Regenerasi Padi Indica melalui Jalur Organogenesis Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Jurnal AgroBiogen Vol 13, No 1 (2017): Juni
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v13n1.2017.p35-42

Abstract

Regenerasi tanaman merupakan tahapan penting yang perlu dikuasai sebelum diaplikasikan untuk perakitan varietas unggul secara kultur in vitro. Penelitian bertujuan mengoptimasi teknik regenerasi in vitro beberapa varietas padi indica melalui jalur organogenesis. Percobaan menggunakan Rancangan Acak Lengkap yang disusun secara faktorial. Materi yang dicobakan, yaitu Ciherang, Inpari 13, Inpara 3, Pokkali, dan IR29. Perlakuan yang diberikan untuk induksi kalus organogenik adalah 2,4-D (0, 1, 3, 5, 7 mg/l) + kasein hidrolisat 3 g/l, untuk regenerasi kalus membentuk tunas adventif adalah BA (0, 1, 5 mg/l) + zeatin (0, 0,1, 0,3 mg/l) + prolin 100 mg/l, untuk multiplikasi tunas adalah MS + thidiazuron/TDZ (0, 0,1, 0,3 mg/l) dan untuk perakar-an adalah IBA (0, 1, 2, 3 mg/l). Hasil penelitian menunjukkan media terbaik untuk induksi kalus untuk padi varietas Ciherang, Inpari 13, dan Pokkali adalah MS + 2,4-D 3 mg/l, sedangkan untuk varietas Inpari 3 dan IR 29 adalah MS + 2,4-D 5 mg/l. Media terbaik untuk regenerasi tunas untuk varietas Ciherang adalah BA 5 mg/l + zeatin 0,1 mg/l + prolin 100 mg/l, untuk Inpari 13 adalah MS + BA 3 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l, untuk Inpara 3 adalah BA 5 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l dan untuk Pokkali dan IR29 adalah MS + BA 3 mg/l + zeatin 0,1 mg/l. Media terbaik untuk multiplikasi tunas adalah MS + TDZ 0,3 mg/l dan untuk induksi perakaran adalah MS + IBA 2 mg/l. Setiap tahapan kegiatan pembentukan planlet membutuhkan formulasi media yang berbeda.
Co-Authors , Kisman , Sukendah , Witjaksono . Wusnah A. Sofwan Achmad . ADI SETIADI Agus Purwito Agustina, Ferra Anggita AHMAD JUNAEDI Ahmad Rifqi Fauzi Ali Djamhuri Almia Permata Putri Amril Aman Andi Dahliaty Aniversari Apriana Aniversari Apriana Aniversari Apriana Ardana, I. N. Kutha Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Aris Purwanto Arrin Rosmala Atmitri Sisharmini Atmitri Sisharmini Atmitri Sisharmini Bambang S. Purwoko Bambang Sapta Purwoko Bhayu Hartanti Bintang, dan Maria Buang Abdullah Cakranegara, Pandu Adi Cucu Sukmana, Cucu Danar Dono Dede J Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede Jajat Sudrajat Dede Kurniadi DEWI SARTIAMI Dewi Sukma Dewi Sukma Didy Sopandie Diny Dinarti Dwi Pratiwi Edi Santosa Efendi, Darda Emmy Darmawati Enny Sudarmonowati Evi Fitriany Faqih Udin Firdausya, Andina Fabrini Freddy Tua Musa Panggabean Gustaaf A Wattimena, Gustaaf A Gustaaf A. Wattimena Gustaaf Adolf Wattimena Gustaaf Adolf Wattimena HADIPOENTYANTI, ENDANG Hartati, RR. Sri Heldiyanti, Rina Hendri Hermawan Adinugraha HUSNI, ALI I Ismayani I MADE ARTIKA Ida Duma Riris Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Roostika Indrastuti Apri Rumanti Irdika Mansur Irfan MARTIANSYAH Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Iswari S Dewi Iswari S. Dewi Iswari Saraswati Dewi Jalaluddin Jalaluddin Kartika Ning Tyas Kartika Ning Tyas Kasutjianingati . Kisman Kisman Kurniawan R. Trijatmiko Kurniawan Rudi Trijatmiko Kurniawan Rudi Trijatmiko La Muhuria Lefin Kafindra Lestari, Risza Putri Lisnawaty Simatupang Lola Adres Yanti Lukman Hakim M Murtadha M. Amir M. Asyabul Zikki Maemonah, Maemonah MARIA BINTANG Marini Damanik Mariska, Ika Maryati Evivani Doloksaribu MEYNARTI SARI DEWI IBRAHIM MEYNARTI SARI DEWI IBRAHIM Muaz Adbdul Karim Muaz Adbdul Karim Muhamad Syukur Muhammad Irfan Habibi MUHAMMAD SYUKUR Mulyatno, Mulyatno Munif Ghulamahdi N. Sri i Hartati Nafilawati wa ode Nata Suharta Natalini Nova Kristina Natalini Nova Kristina Nataniel Tandirogang Nugroho, Candra Catur Nura ,, Nura Nurbaiti Nurbaiti Nurhasybi Nurhasybi Nurul Fatimah Nurul Fauziah O K Sofyan OTIH ROSTIANA OTIH ROSTIANA Pienyani Rosawanti Rahmi Henda Yani Rasyad, M. Ashadi Reny Herawati Rina Hapsari Wening, Rina Hapsari Rinda Cahyana Riyanti Catrina H S Riza Arief PUTRANTO Rizkiannur Putri, Amalia Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rozanna Dewi Ryan Budi Setiawan Sadewi Maharani Sadewi Maharani, Sadewi Saepudin, Adam Safitri Safitri Saronom S ilaban Sarwono Hardjowigeno Shafwan Fahmi Silawati, Tutisiana Silawati Tutisiana Sintho Wahyuning Ardie Siregar, Ulfah J Siswati, Leni Siti Aisyah Siti Kurniawati Slamet Budijanto Slamet Susanto Sobir Sobir Sri Enny Triwidiastuti Sri Helianty Sry Rahmadani Subekti, Isnani Sudarsono Sudarsono Sudarsono Suhesti, Sri Sukma, Dewi Sulastri Sulastri Sulhatun Sulhatun SUPRIADI SUPRIADI Suryati Suryati Suryo Wiyono Sutalaksana, Iftikar Z Sutrisno Mardjan Sutrisno, Sutrisno Suyanti Kasimin Syamsul Bahri Syamsul Bahri Tarigan, Asmara Iriani Tengku Mia Rahmiati Teti Arabia Thifany, Ariny Jihan Tri Handayani Tri Joko Santoso Tri Lestari Mardiningsih Tri Lestari Mardiningsih, Tri Lestari Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Trismi Ristyowati Ulfah J. Siregar Ulfah Juniarti Siregar Ummu Kalsum Wahyuning Ardie, dan Sintho Waizul Fahri Purba Waras Nurcholis Warid Warid Wattimena, and Gustaaf Adolf WATTIMENA, G. A. Wening Enggarini Wening Enggarini Widiatmaka Widodo , Widowati, Sartika Widya Hartati, Widya Wijayanti, Mustika Eka Willy Bayuardi Suwarno Yudiansyah Yudiansyah Yulia, Endah Yulia Yundari, Yundari Yunita, Rossa Zuli Nuraeni