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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Jurnal Teknologi Pangan dan Gizi Jurnal Penelitian Pascapanen Pertanian Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Indonesian Journal of Biotechnology Animal Production : Indonesian Journal of Animal Production Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Livestock and Animal Research Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research

Denny Widaya Lukman

Divisi Kesehatan Masyarakat Veteriner, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680

Author-ID : 309447

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Indirect Enzyme Linked Immunosorbent Assay Sebagai Metode untuk Melacak Bruselosis pada Sapi Perah (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW) Rinaldi Ghurafa; Denny Widaya Lukman; Hadri Latif
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated sensitivity and specificity, as well as analyzed by calculating the kappa value. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated for sensitivity and specificity, as well as analyzed by calculating the Kappa statistical value. The results of the sensitivity and specificity calculation showed that the indirect enzyme linked immunosorbent assay (I-ELISA) test developed a higher sensitivity (100%) compared to RBT test (93.75%) and commercial I-ELISA (93.75%). The developed I-ELISA specificity (74.68%) was still lower than RBT (89.87%), but higher than commercial I-ELISA (70.52%). The calculation of the statistical value of kappa RBT with CFT showed the kappa value 0.7120 which meaned it had a good agreement, commercial I-ELISA with CFT showed kappa value 0.6165 which meaned it had good suitability, whereas I-ELISA developed with CFT showed kappa value 0.4984 which meaned having a moderate agreement.In conclusion, the indirect enzyme linked immunosorbent assay (I-ELISA) which had been developed had low specificity, but the sensitivity was the highest compared to the commercial I-ELISA test and RBT, so this test was appropriate to be used as a screening test, especially in dairy cows movement into brucellosis-free areas or regions.

Keberadaan Bakteri Listeria monocytogenes pada Keju Gouda Produksi Lokal dan Impor (PRESENCE OF LISTERIA MONOCYTOGENES IN LOCAL AND IMPORTED GOUDA CHEESES) Debby Fadhilah Pazra; Trioso Purnawarman; Denny Widaya Lukman
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Listeria monocytogenes is included in the foodborne pathogen, which has been associated with severaloutbreaks of human listeriosis especially in high risk groups. Listeria monocytogenes could be found inGouda cheeses because of poor hygienic and sanitation practices. In addition, this bacteria could surviveduring the making of cheese and cheese ripening process. The purpose of this study was to identify thepresence of L. monocytogenes in local and imported Gouda cheeses and how the safety level of the Goudacheese against contamination of L. monocytogenes. This study used the conventional method in accordancewith the Bacteriological Analytical Manual, US Food and Drug Administration and Bergey’s Manual ofDeterminative Bacteriology to detect the presence of L. monocytogenes at 15 samples of local Gouda cheeseand 15 samples of imported Gouda cheese sold in supermarkets in Jakarta and Bogor. The results of thisstudy showed that was not found L. monocytogenes in local and imported Gouda cheese. It could be concludedthat is Gouda cheese relatively safe from L. monocytogenes and meets Indonesian National Standard.

Cemaran Escherichia coli pada Tepung Telur yang Diimpor Melalui Pelabuhan Tanjung Priok, dan Resistensinya Terhadap Antibiotik (ESCHERICHIA COLI CONTAMINATION ON EGG POWDER IMPORTED THROUGH PORT OF TANJUNG PRIOK AND ITS RESISTANCE AGAINST ANTIBIOTICS) Kamil Riski Sidik; Denny Widaya Lukman; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Eschericia coli contamination was found on egg powder imported to Indonesia during microbialexamination. The study was conducted to describe the presence of E. coli and its resistance to antibioticsin egg powder imported to Indonesia through The port of Tanjung Priok. Samples was determined usingcross sectional study and the sample size was calculated based on assumption of confidence level of 95%with margin of error of 10% and predicted prevalence of 50%. Total of 100 egg powder samples wascollected in August 2014 from two exporting countries, Ukraine (whole egg powder, n=30) and India (wholeegg powder, n=40 and egg yolk powder, n=30). Examination was performed by packaging and label inspectionof the product followed by samples collection and testing for bacterial contamination using rapid test andconventional isolation and identification methods. Detected E. coli was isolated and then tested forantibiotic resistance. Examination results showed that 4 samples were positive to E. coli. E. coli isolatesshowed resistance against 6 types of antibiotics and 75% of the isolates had resistance against minimumof 3 types of antibiotics. These conditions should be taken into consideration since antibiotic resistance inE. coli would cause negative impacts on human, animal and environmental health.

Identifikasi Escherichia coli yang Resistan Antibiotik pada Daging Burger yang Dijual di Sekitar Kampus IPB Dramaga Bogor Kumala Andri Asari; Denny Widaya Lukman; Trioso Purnawarman
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mengetahui cemaran Escherichia coli dan resistansinya terhadap antibiotik pada sampel daging burger yang dijual pada radius 2 km batas Kampus Institut Pertanian Bogor di Dramaga, Bogor. Sebanyak 35 sampel daging burger diperoleh dari semua pedagang burger (tujuh pedagang). Isolasi dan identifikasi E. coli mengacu pada Standar Nasional Indonesia 2897:2008. Uji resistansi terhadap antibiotik menggunakan metode Kirby-Bauer dengan acuan dari Clinical Laboratory Standards Institute tahun 2018. Uji resistansi antibiotik dilakukan terhadap semua isolat E. coli yang didapat dari semua koloni E. coli yang diisolasi dari sampel daging burger. Antibiotik yang digunakan dalam penelitian ini adalah ampisilin, amoksisilin, streptomisin, oksitetrasiklin, tetrasiklin, sefotaksim, gentamisin, enrofloksasin, asam nalidiksat, kloramfenikol, dan trimetoprim-sulfametoksasol. Data dianalisis secara deskriptif. Hasil pengujian menunjukkan11,4% (4 dari 35 sampel) positif E. coli. Isolat yang diperoleh dari empat sampel ada lima isolat E. coli (100%) yang resistan terhadap antibiotik yang diuji. Resistansi tertinggi ditemukan pada antibiotik streptomisin (100%) dan gentamisin (80%). Dari lima isolat E. coli yang didapat, empat isolat di antaranya telah resistan terhadap tiga atau lebih golongan antibiotik yang dikenal sebagai multi-drug resistant (MDR), dengan pola resistansi yang terdiri atas AMP-AMC-S-CN (ampisilin-amoksisilin-streptomisin-gentamisin), AMP-S-ENR-NA-SXT-CN (ampisilin-streptomisin-enrofloksasin-asam nalidiksat-trimetoprim sulfametoksasol-gentamisin), dan AMP-S-ENR-NA-SXT-OT-CN (ampisilin-streptomisin- enrofloksasin-asam nalidiksat- trimetoprim sulfametoksasol-oksitetrasikilin-gentamisin). Keberadaan E. coli resistan pada daging burger yang termasuk pangan siap santap (ready-to-eat food) dapat menimbulkan risiko kesehatan pada konsumen.

Seroprevalensi Positif Sistiserkosis pada Babi Hutan di Kabupaten Way Kanan, Provinsi Lampung (POSITIVE SEROPREVALENCE OF WILD BOAR CYSTICERCOSIS IN WAY KANAN DISTRICT, LAMPUNG PROVINCE) Heri Yulianto; Fadjar Satrija; Denny Widaya Lukman; Mirnawati Sudarwanto
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Wild boar is also an intermediate host of Taenia solium, besides domestic pig which is the mainsource of Cysticercus infection in human. Cysticercosis in wild boar has not been reported in Indonesia.The aim of this study was to determine wild boar’s cysticercosis in Way Kanan District. One hundred serasamples consisting of 41 male wild boars and 59 female wild boars were collected. Eighty seven of boarscome from Banjit Subdistrict, while 13 of wild boars come from Blambangan Umpu Subdistrict in WayKanan District. The serum were tested serologically to determine antigen of parasite cycles. The test usedmonoclonal antibody-base sandwich Enzyme-Linked Immunosorbent Assay (Moab-ELISA). There is asero positive reaction of cysticercosis(1%) which is female wild boar from Banjit sub district. Controllingof meat inspection should be conducted to prevent cysticercosis transmission to human.

Faktor Risiko Babi yang Diumbar dan Pakan Mentah Mempertinggi Prevalensi Sistiserkosis (RISK FACTOR OF FEE-RANGE HUSBANDRY AND RAW PORK FEED INCEASE THE PREVALENCE OF CYSTICERCOSIS) Inriyanti Assa; Fadjar Satrija; Denny Widaya Lukman; Nyoman Sadra Dharmawan; Pierre Dorny
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aims of this study were to determine the prevalence and risk factors of porcine cysticercosis. Thesurvey was carried out in eight districts of Jaya Wijaya, Papua and Jibama market, during October 2009to June 2011. A total of 111 pigs were tested serologically. Serum samples were tested for the presence ofcirculating parasite antigen using monoclonal antibody-based sandwich enzyme-linked immunosorbentassay (MoAb-ELISA). Forty five samples (40.54%) were found positive by MoAb-ELISA and the highestprevalence occurred from the District of Asolokobal (92.86%), followed by Musatfak (75%), Kurulu (65.22%),Bolakme (33.33%), Asologaima (31.82%), Hom-hom (18.18%), Hubikosi (14.29%), Jibama trade (14.29%),and the lowest prevalence from Wamena Kota is 5.88%. Free-range pig husbandry system (OR=4.63;P<0.01) and uncook pork feed (OR=3.65; P<0.05) were important risk factors for porcine cysticercosis. It istherefore necessary to anthropology approach about pig husbandry system and pattern of cook pork feed.

Pengetahuan, Sikap, dan Praktik Pemilik Anjing Terkait Rabies di Kabupaten Sukabumi, Jawa Barat (KNOWLEDGE, ATTITUDE, AND PRACTICE STUDY OF DOG OWNERS RELATED TO RABIES IN SUKABUMI DISTRIC, WEST JAVA) Ardilasunu Wicaksono; Abdul Zahid Ilyas; Etih Sudarnika; Denny Widaya Lukman; Yusuf Ridwan
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Sukabumi District is one of the districts in West Java Province that has not been rabies-free since there are still reported rabies dog bites and rabies-positive cases in humans. The aim of the study was to measure the knowledge, attitude and practice of dog’s owner towards rabies which will then be useful for designing the appropriate rabies control programs. A total of 141 dog owners were selected as samples/ respondents. Respondents were taken from two sub-districts in Sukabumi District, which were 66 people from Jampang Tengah Sub-district and 75 people from Cisolok Sub-district. The data were taken using structured questionnaire. The result showed that most of respondent’s level of knowledge (51.1%) was in moderate category, meanwhile the respondent’s level of attitude related to rabies control program mostly (69.5%) was in positive category. But the positive attitude was not reflected with their practice towards rabies control program which many of them (63.1%) was in bad category of rabies control practices. From analytical result showed that only one characteristic variablewhich was the information access about rabies that had significant correlation (c2=9.959, p=0.006) towards respondent’s level of knowledge. Furthermore, another result showed that there was a significant relationship between knowledge variable with respondent’s attitude level (p= 0.000, r= 0.275).

Pengembangan Enzyme-Linked Immunosorbent Assay Paratuberkulosis dengan Antigen Protoplasmik Mycobacterium avium Subspecies Paratuberculosis Isolat Lapang (DEVELOPMENT OF PARATUBERCULOSIS ENZYME-LINKED IMMUNO-SORBENT ASSAY WITH PROTOPLASMIC ANTIGEN OF MYCO Rahmat Setya Adji; I Wayan Teguh Wawan; Denny Widaya Lukman; Surachmi Setiyaningsih
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Enzyme-linked immunosorbent assay (ELISA) is a serological test method most widely used for thediagnosis of paratuberculosis, because it has a better sensitivity compared to other serological test.Protoplasmic antigen (PPA) or cellular extract is still the main choice for the diagnosis of paratuberkulosisdevelopment. The aim of research was to use the PPA Mycobacterium avium subspeciesparatuberculosis(MAP) field isolates for the development of paratuberculosis ELISA (ELISA PPA-L). As many as 322cattle sera (300 negative and 22 positive) were tested using this method and compared with IDEXXcommercial kit. The sensitivity and specificity of ELISA PPA-L test results were 68.18% and 97.0%,whereas for the IDEXX kit were 63.64% and 97.33%respectively. ELISA PPA-L had higher sensitivity andlower specificity compared to the IDEXX commercial kit. ELISA test using protoplasmic antigen of MAPfield isolates has good ability for paratuberculosis serological test and can be used for screening test of thedisease in Indonesia.

Resistensi Antibiotik pada Salmonella Isolat Sapi Bakalan Asal Australia yang Diimpor Melalui Pelabuhan Tanjung Priok Jakarta (ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTED FEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) Anindya Kurniawati; Denny Widaya Lukman; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Importation of feeder cattle from farms constantly using antibiotics in feed could result in the occurrenceof antibiotic resistant bacteria. A study was therefore conducted to investigate the possible use antibioticsand the antibiotic resistance of commensal Salmonella sppagainst several antibiotics. Salmonella spp.bacteria (n=50)were isolated from 100 samples of feeder cattlesfaeces. Total of 50 Salmonella spp. isolateswere subjected for Salmonella spp. examination and the isolated Salmonella spp. was tested for theantibiotic resistance using 10 antibiotics (ampicillin, cephalotin, erythromycin, tetracycline, streptomycin,chloramphenicol, trimethoprim, trimethoprim-sulfamethoxazole, nalidixic acid, and enrofloxacin) usingdisk diffusion method on Muller-Hinton agar following Clinical and Laboratory Standards Institute (CLSI)guidelines for interpretation. The isolated Salmonella showed resistance towards erythromycin 98%,ampicillin 34%, streptomycin 22%, nalidixic acid 8%, cephalotin 6%, tetracycline 4%, and chloramphenicol2%. There was no resistance against enrofloxacin, trimethoprim, and trimethoprim-sulfamethoxazole. Salmonella spp. bacteria derived from imported feeder cattle which have been resistant to antibiotics arepotential for spreading the antibiotic resistant bacteria to other susceptible animals. Such bacteria canalsotransfer the antibiotic resistant gene to other bacteria in Indonesia which ould be a potential threatfor public and animal healths.

ISOLASI DAN IDENTIFIKASI ESCHERICHIA COLI O157:H7 PADA DAGING SAPI DI KABUPATEN BADUNG PROVINSI BALI. ISOLATION AND IDENTIFICATION ESCHERICHIA COLI O157:H7 ON BEEF AT BADUNG REGENCY PROVINCE OF BALI I Wayan Suardana; Bambang Sumiarto; Denny Widaya Lukman
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Keamanan bahan pangan merupakan masalah yang amat penting bagi konsumen dan industri pangan. Cemaran bakteri Escherichia coli dan Coliform dianggap sebagai indikator sanitasi dalam proses pengolahan bahan pangan. Pelacakan bakteri patogen dalam pangan juga telah dilakukan secara rutin, termasuk yang bersifat zoonosis seperti Escherichia coli O157:H7. Bakteri ini menghasilkan toksin yang dikenal dengan Shiga toxin. Toksin ini dapat menimbulkan diare berdarah, colitis haemorrhagi dan hemolytic uremic syndrome (HUS) pada manusia. Dalam penelitian ini dipelajari hubungan antara tingkat cemaran dan insidensi Coliform, E.coli, E.coli O157 dan E.coli O157:H7 pada daging sapi. Bakteri pertama ditumbuhkan pada media EMBA, selanjutnya dipupuk pada media SMAC dan diakhiri dengan uji aglutinasi lateks untuk memastikan keberadaan bakteri E.coli O157 dan uji antiserum H7 untuk memastikan isolat yang diisolasi merupakan isolat E.coli O157:H7. Hasil isolasi dan identifikasi terhadap 89 sampel daging sapi diperoleh hasil rata-rata tingkat cemran coliform dan E.coli sebesar 93,01+ 2,64x103 cfu/g dan

Co-Authors Abdul Zahid Abdulwahed Ahmed Hassan Aditya Primawidyawan Afiff , Usamah Agus Wijaya Agustin Indrawati Ahmad Nurhakim Aminudin, Muhammad Rizki Andrijanto Hauferson Angi Anindya Kurniawati Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Asah Hilaliah Bambang Pontjo Priosoeryanto Bambang Sumiarto Chaerul Basri Chandra Utami Wirawati Debby Fadhilah Pazra Devi Yanti Sari Edi Sukmawinata Eko Agus Srihanto Ekowati Handharyani Engki Zelpina Erfiandini Eka Puspita Etih Sudarnika Eulis Tanti Marlina Ewald Usleber Fachriyan Hasmi Pasaribu Hadri Latif Hasudungan A Sidabalok Hasudungan A Sidabalok, Hasudungan A Heri Yulianto Herwin Pisestyani I Gusti Made Krisna Erawan I Wayan Suardana I Wayan Teguh Wawan I wayan Teguh Wibawan IETJE WIENTARSIH Indra Permana, Indra Inriyanti Assa Irena Ivania Kallau, Novalino Harold Geoffrey Kamil Riski Sidik koekoeh santoso Kumala Andri Asari Loisa Loisa Loisa, Loisa Manuel da Costa Maya Shofa Milawarni Milawarni Minarwati Sudarwanto Mirnawati B Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Naoaki Misawa Nararya Adinata Nugraha, Arifin Budiman Pierre Dorny Platika Widiyani Putra Wijaya, Erlangga Putut Isworo Arimurti Rahmat Setya Adji Rakhmi Ros Sari Ramadhani, Nada Nursaffana Razali Razali Rinaldi Ghurafa Rismayani Saridewi Rismayani Saridewi, Rismayani Risqika Akla Velayati Rochman Naim Ronald Tarigan Roostita L Balia Rotinsulu, Dordia Anindita Saukhan, Falih Prenata Sekarsana, Disty Ayu Siti Gusti Ningrum sri murtini . Sudarwanto, Mirnawati Bachrum Sukmawinata, Eddy Supriyono Supriyono Surachmi Setiyaningsih Surachmi Setyaningsih Surachmi Setyaninigsih Tangkonda, Elisabet Taufiqqurrahman, Mas Trioso Purnawarman Umi Cahyaningsih Widagdo Sri Nugroho Woro Wulandari Kalanjati Wyanda Arnafia Yusuf Ridwan

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