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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Jurnal Teknologi Pangan dan Gizi Jurnal Penelitian Pascapanen Pertanian Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Indonesian Journal of Biotechnology Animal Production : Indonesian Journal of Animal Production Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Livestock and Animal Research Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Denny Widaya Lukman
Divisi Kesehatan Masyarakat Veteriner, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Profil hematologi anjing lokal di wilayah endemik rabies Agus Wijaya; Ronald Tarigan; Koekoeh Santoso; Yusuf Ridwan; Etih Sudarnika; Abdul Zahid Ilyas; Denny Widaya Lukman; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Sri Murtini; Edi Sukmawinata; Annisa Madyanti Geminastiti Parampasi
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.224 KB) | DOI: 10.29244/avl.2.1.1-2

Abstract

Provinsi Jawa Barat merupakan satu dari 24 provinsi di Indonesia yang dilaporkan belum bebas Rabies. Usaha pembebasan wilayah Jawa Barat dari penyakit Rabies sangat strategis mengingat posisi Jawa Barat berada di antara daerah bebas Rabies. Jampang Tengah menjadi salah satu kecamatan di Sukabumi yang pada tahun 2016 dilaporkan telah terjadi 13 kasus gigitan Rabies. Penelitian ini menggunakan 81 sampel darah anjing lokal yang dikoleksi dari enam desa di kecamatan Jampang Tengah dan diperiksa dengan menggunakan alat Rayto® hematology analyzer. Parameter profil hematologi dikelompokkan berdasarkan perbedaan umur dan jenis kelamin anjing. Hasil pemeriksaan hematologi secara umum berdasarkan umur menunjukkan nilai rataan yang lebih besar pada anjing lokal umur ≥1 tahun dan konsentrasi leukosit menunjukkan nilai statistik yang signifikan berbeda (p<0.05). Berdasarkan jenis kelamin, nilai rataan parameter hematologi secara umum lebih tinggi pada anjing lokal betina dan adanya perbedaan signifikan (p<0.05) pada konsentrasi leukosit dan granulosit.
STUDI PEMETAAN MULTI-DRUG RESISTANT (MDR) ESCHERICHIA COLI PADA PETERNAKAN BABI DI KOTA KUPANG Novallino Kallau; I Wayan Teguh Wibawan; Denny Widaya Lukman; Mirnawati Baharudin Sudarwanto
JURNAL KAJIAN VETERINER Vol 7 No 1 (2019): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v7i1.946

Abstract

The aim of this study was to determine the pattern of the spread of antibiotic-resistant E. coli on pig farms in Kupang City by using mapping analysis. Data on E. coli resistance comes from the results of laboratory analysis and as secondary data for spatial analysis. Spatial analysis uses the nearest neighbour index, convex hull and elementary analysis of disease methods. The results of this study have shown a high prevalence of E. coli (85.4%) with clustered distribution patterns and have a wide spread (10920 ha) in the area in Kupang City. MDR E. coli had a moderate prevalence (57.31%) by forming a group spread pattern and a wide spread (7778 Ha) on pig farms in Kupang City. This result encourages prevention and control efforts by all interested parties so that the rate of spread of resistant E. coli can be reduced.
Profil Peternakan Babi Di Kota Kupang Dan Potensi Penularan Trichinellosis Andrijanto Hauferson Angi; Fadjar Satrija; Denny Widaya Lukman; Mirnawati Sudarwanto; Etih Sudarnika
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.997

Abstract

Trichinellosis is a parasitic disease of humans caused by eating raw from domestic or game animals infected by Trichinella spp. Human trichinellosis contracted from commercial supplies of meat have been most often linked to infected pigs, wild boar, or horses. Trichinella is a nematode which has an atypical direct life cycle that does not involve stages developing outside of the host. This study was conducted to see the profile of pig farms in the city of Kupang and the potential transmission of trichinellosis. The data was derived from interview 60 farmers in 6 sub districts in city of Kupang by using a structured questionnaire and analyzed descriptively. The results of the questionnaire survey showed that many race of pig from a mixed race, the seeds come from traditional breeding. Feed rest of the home or restaurant are usually directly given to the pigs. The presence of rat in around of the cage often. All respondents were interviewed did not know or hear about trichinellosis disease which can be one cause of the spread of trichinellosis in city of Kupang.
Kualitas Mikrobiologis Daging Ayam yang Dijual di Pasar Kota Dili, Timor Leste Manuel da Costa; Mirnawati Bachrum; Denny Widaya Lukman; Herwin Pisestyani; Etih Sudarnika
Acta VETERINARIA Indonesiana Vol. 10 No. 2 (2022): Juli 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.2.172-181

Abstract

Daging ayam merupakan salah satu produk pangan asal hewan yang banyak dikonsumsi di Timor Leste. Salah satu aspek kualitas daging ayam yang harus diperhatikan adalah kualitas mikrobiologi. Penelitian ini bertujuan untuk mengetahui kondisi umum penjualan daging ayam dan kualitas mikrobiologis daging ayam berdasarkan jumlah hitungan cawan (Total Plate Count/TPC) dan jumlah Enterobacteriaceae pada daging ayam lokal maupun impor yang dijual di Pasar Kota Dili, Timor Leste. Penelitian dilakukan pada 8 pasar dengan sampel sebanyak 150 yang terdiri dari 60 sampel daging ayam lokal dan 90 daging ayam impor. Metode TPC dan jumlah Enterobacteriaceae dilakukan berdasarkan SNI Nomor 2897 Tahun 2008 tentang Metode Pengujian Cemaran Mikroba dalam Daging, Telur dan Susu, serta Hasil Olahannya. Menurut International Commission on Microbiological Specification for Foods (ICMSF) tentang Kriteria Mikrobiologi dalam Pangan jumlah maksimum nilai TPC dan jumlah Enterobacteriaceae setiap bahan makanan masing-masing adalah 7 log cfu/g dan <102 cfu/g (toleransi negara tertentu 102–104 cfu/g). Nilai rata-rata TPC dan jumlah Enterobacteriacea daging ayam yang beredar di Timor Leste berdasarkan penelitian ini masing-masing 4.78 ± 0.90 log cfu/g (3.05 × 105 ± 5.66 × 105 cfu/g) dan 3.34 ± 0.93 log cfu/g (2.86 × 104 ± 9.93 × 104 cfu/g). Kualitas mikrobiologi seluruh jenis daging ayam yang beredar di Timor Leste memenuhi standar yang ditetapkan oleh ICMSF berdasarkan nilai rata-rata TPC. Sementara itu, berdasarkan jumlah Enterobacteriaceae, hanya daging ayam lokal dan daging ayam impor dari Brazil memenuhi standar jumlah Enterobacteriaceae yang ditetapkan oleh ICMSF. Berdasarkan analisis statistik, maka kualitas mikrobiologis daging ayam yang dijual di Pasar Kota Dili, Timor Leste berdasarkan TPC dan jumlah Enterobacteriaceae dapat disimpulkan yang terbaik adalah daging ayam lokal dan daging ayam impor dari Brazil, sedangkan kualitas yang kurang baik adalah daging ayam lokal dan daging ayam impor dari Malaysia, dan impor dari Amerika. Tingginya TPC dan jumlah Enterobacteriaceae pada daging ayam yang dijual mengindikasikan bahwa praktik higiene sanitasi di pasar perlu diperbaiki.
Keberadaan bakteri multiresisten Escherichia coli penghasil ESBL/AmpC, Klebsiella oxytoca dan Kluyvera spp. diisolasi dari lingkungan ayam rumah potong hewan di Pondok Rumput, Bogor, Indonesia: Keberadaan bakteri multiresisten Escherichia coli penghasil ESBL/AmpC, Klebsiella oxytoca dan Kluyvera spp. diisolasi dari lingkungan ayam rumah potong hewan di Pondok Rumput, Bogor, Indonesia Woro Wulandari Kalanjati; Denny Widaya Lukman; Mirnawati Sudarwanto
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 12 No 3 (2022): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.12.3.472-477

Abstract

In the last few years, multidrug resistant bacteria increased significantly and contaminated chicken carcass through production chain. This study identified the presence of ESBL/AmpC-producing Enterobactericeae collected from the environment of chicken slaughterhouses. Samples were taken before slaughtering process from several points of determined locations. From a total of 84 samples tested, 30 isolates were positive of Enterobactericeae comprising of 22 isolates of E. coli, 6 isolates of Klebsiella oxytoca, and 1 isolate of Kluyvera spp. Confirmation test of ESBL/AmpC phenotypic and antibiotic resistant test to 18 kinds of antibiotics were conducted using MASTDISCS D68C commercial kit and Kirby-Bauer disc diffusion susceptibility test with CLSI (2014) interpretation. All of isolates showed resistance to penicillin, ampicillin, amoxicillin, cefotaxime, cefpodoxime, ceftazidime and some isolates show resistance to streptomycin, gentamicin, trimethoprim-sulphametoxasol, tetracycline, kanamycin, doxycyclin, colistin sulphate, cephalothin, neomycin and polymyxin B. This study demonstrated the existence of ESBL/AmpC-producing Enterobactericeae that were found on the carcasses container, offal container, floors of carcasses, offal handling area, knives and feathers puller machine. The existence of resistant bacteria in the environment of chicken slaughterhouse can lead to occurrence of antibiotic resistant and transmission of resistance genes to other bacteria that could spread to the carcass that was potentially harmful to humans.
Gambaran Escherichia coli resistan antibiotik asal tangan pemerah, ambing sapi, susu serta air di Peternakan Sapi Perah Kecamatan Cendana, Enrekang, Sulawesi Selatan Milawarni Milawarni; Herwin Pisestyani; Denny Widaya Lukman
Livestock and Animal Research Vol 20, No 3 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v20i3.61288

Abstract

Objective: This study aimed to describe the resistance of E. coli to several types of antibiotics isolated from water, milker hand swabs, cow udder swabs and milk in the Enrekang dairy farm, South Sulawesi.Methods: Isolation and identification of E. coli refers to SNI 2897:2008 concerning Methods for Testing Microbial Contamination in Meat, Eggs and Milk, and their Processed Products. The test of E. coli resistance to antibiotics by disc diffusion method and interpretation of the results refers to the Clinical and Laboratory Standards Institute (CLSI). The samples were 13 milker hand swabs, 52 cow udder swabs, 52 individual milks, and 13 water used for rearing activities.Results: E. coli isolates (n=64) consisted of 7 isolates from hand swabs, 7 isolates from milking hands, 20 isolates from udder swabs, 31 isolates from milk, and 6 isolates from water. E. coli isolates were resistant to erythromycin and ampicillin as much as 78%, trimethoprim-sulfamethoxazole 34.4%, tetracycline 31%, ceftazidime 25%, chloremphenicol 22%, enrofloxacin 9.4%, and gentamicin 4.6%. The pattern of E. coli resistance to antibiotics showed that the isolates of E. coli multiple drugs were resistant to up to 7 antibiotics at once.Conclusions: E. coli isolates from milk, cows, milk and water in the Enrekang dairy farming area, South Sulawesi has developed resistance to several types of antibiotics and have the potential to spread resistance genes to other bacteria and threaten human, animal and environmental health.
Microbiological Quality of Milk Flavored Ready to Drink and Hygiene Practice of Street Vendors Herwin Pisestyani; Irena Ivania; Ardilasunu Wicaksono; Denny Widaya Lukman; Mirnawati Sudarwanto
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60996

Abstract

Milk products consumption has become a new trend of healthy lifestyle among students in order to meet appropriate daily nutrients. This study was aimed to measure the total number of microbes found in milk drinks sold around the IPB Dramaga and Cilibende and to determine factors that influence it. Samples were collected from 13 milk vendors within radius of 2 kilometers from outermost point of campus and taken with 3 repetitions. Calculation of the total number of microbes was done by using the plate count method in accordance with SNI 2897-2008 about testing method of microbial contamination in meat, eggs, and milk, as well as processed products and SNI 01-6366-2000 about the maximum limit of microbial contamination in food. Risk factors’ data was taken by interviewing milk drinks seller using structured questionnaire. Factors that influenced microbial contamination were analyzed using Chi-Square test. The results showed that 74.4% of milk drinks samples had a high total number of microbes. The average total number of microbes in Dramaga and Cilibende samples was 1.0 x 105 ± 1.4 x 105 cfu / ml and 1.1 x 107 ± 1.2 x 107 cfu/ml. The total number of microbes are significantly influenced (p<0.05) by equipments used, the cleanliness of the equipment, the cleanliness of the table, washing hands practice, and the distance from the crowd. The high number of microbial contamination in milk drinks could damage the quality of milk and shorten the shelf life.
Presence of Escherichia coli O157:H7 in Chicken Meat at Traditional Markets in Pangkalpinang City Ahmad Nurhakim; Denny Widaya Lukman; Herwin Pisestyani
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.71162

Abstract

This study was aimed to identify the presence of E. coli O157:H7 and analyze the association between factors and frequency of E. coli in chicken meat sold at Kaget Market, Pagi Market, and Pembangunan Market in Pangkalpinang City.  A total of 30 chicken meat samples were collected proportionally using simple random sampling.  This study was used a cross sectional method.  The examination of E. coli was carried out based on SNI 2897:2008 concerning The Testing Method of Microbial Contamination in Meat, Eggs, and Milk and Their Processed Products.  The E. coli O157 was confirmed with O157 latex agglutination test and H7 antiserum test.  The data were analyzed using a descriptive test and risk factors were analyzed using chi-square and odds ratio.  The results showed that E. coli O157:H7 was found in 2 of 3 samples (66,7%) isolated from Kaget Market.  The significant risk factors (P<0,005) for the presence of E. coli were traders with age of more 45 years, kiosk distance of less than 5 meters from the highway, and washing hands using unclean water.  The presence of E. coli O157:H7 will be a threat for consumer health.
RESISTENSI Salmonella spp. TERHADAP BEBERAPA ANTIBIOTIK PADA DAGING ITIK DI KABUPATEN BOGOR YANG DAPAT MEMENGARUHI KESEHATAN KONSUMEN (Resistance of Salmonella spp. to Several Antibiotics from Duck Meat in Bogor District that Could Influence Consumer Health) Loisa Loisa; Denny Widaya Lukman; Hadri Latif
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.189 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5040

Abstract

This study aimed to determine the presence of Salmonella spp. in duck meat and to identify the resistance of Salmonella spp. against several antibiotics in duck meat associated with food safety. Total of 52 meat samples of ducks was collected from 5 subdistricts in Bogor District, i.e., Cariu-Jonggol, Gunung Sindur, Klapanunggal, Parung Panjang, and Ciomas based on the assumption of 95% confidence level, 50% predicted prevalence, and 10% standard error. The results showed that three samples (5.8%) were positive Salmonella spp. The majority of Salmonella spp. isolated from duck meat showed resistance against erythromycin (66.7%), streptomycin (33.3%), and chloramphenicol (33.3%). Nevertheless, Salmonella spp. was still sensitive againts enrofloxacin, trimethoprim sulfamethoxazole, cephalothin, ampicillin, nalidixid acid, tetracycline, and gentamicin.
DAYA TAHAN HIDUP Toxoplasma gondii DALAM SUSU KAMBING SETELAH PASTEURISASI SUHU TINGGI WAKTU SINGKAT Rismayani Saridewi; Denny Widaya Lukman; Mirnawati Sudarwanto; Umi Cahyaningsih
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.33 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2824

Abstract

Tujuan dari penelitian adalah menetapkan daya tahan hidup takizoit Toxoplasma gondii galur RH dalam susu kambing setelah dipasteurisasi pada suhu tinggi dalam waktu singkat. Dalam penelitian ini digunakan metode in vivo dan mencit diinfeksi dengan takizoit Toxoplasma gondii galur RH secara intraperitoneal dengan konsentrasi 2,76x106 takizoit/ekor. Mencit dibagi atas tiga kelompok perlakuan, yaitu susu pasteurisasi dan takizoit yang dipanaskan pada suhu 72 °C selama 15 detik (P), susu pasteurisasi dan takizoit tanpa dipanaskan sebagai kontrol positif (KP), dan  susu pasteurisasi tanpa takizoit sebagai kontrol negatif (KN). Hasil penelitian menunjukkan bahwa tidak ditemukan takizoit di dalam cairan peritoneal pada P dan KN. Takizoit Toxoplasma gondii galur RH ditemukan pada KP yang mempunyai jumlah konsentrasi hampir sama sebelum dan setelah infeksi.
Co-Authors Abdul Zahid Abdulwahed Ahmed Hassan Aditya Primawidyawan Afiff , Usamah Agus Wijaya Agustin Indrawati Ahmad Nurhakim Aminudin, Muhammad Rizki Andrijanto Hauferson Angi Anindya Kurniawati Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Asah Hilaliah Bambang Pontjo Priosoeryanto Bambang Sumiarto Chaerul Basri Chandra Utami Wirawati Debby Fadhilah Pazra Devi Yanti Sari Edi Sukmawinata Eko Agus Srihanto Ekowati Handharyani Engki Zelpina Erfiandini Eka Puspita Etih Sudarnika Eulis Tanti Marlina Ewald Usleber Fachriyan Hasmi Pasaribu Hadri Latif Hasudungan A Sidabalok Hasudungan A Sidabalok, Hasudungan A Heri Yulianto Herwin Pisestyani I Gusti Made Krisna Erawan I Wayan Suardana I Wayan Teguh Wawan I wayan Teguh Wibawan IETJE WIENTARSIH Indra Permana, Indra Inriyanti Assa Irena Ivania Kallau, Novalino Harold Geoffrey Kamil Riski Sidik koekoeh santoso Kumala Andri Asari Loisa Loisa Loisa, Loisa Manuel da Costa Maya Shofa Milawarni Milawarni Minarwati Sudarwanto Mirnawati B Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Naoaki Misawa Nararya Adinata Nugraha, Arifin Budiman Pierre Dorny Platika Widiyani Putra Wijaya, Erlangga Putut Isworo Arimurti Rahmat Setya Adji Rakhmi Ros Sari Ramadhani, Nada Nursaffana Razali Razali Rinaldi Ghurafa Rismayani Saridewi Rismayani Saridewi, Rismayani Risqika Akla Velayati Rochman Naim Ronald Tarigan Roostita L Balia Rotinsulu, Dordia Anindita Saukhan, Falih Prenata Sekarsana, Disty Ayu Siti Gusti Ningrum sri murtini . Sudarwanto, Mirnawati Bachrum Surachmi Setiyaningsih Surachmi Setyaningsih Surachmi Setyaninigsih Tangkonda, Elisabet Taufiqqurrahman, Mas Trioso Purnawarman Umi Cahyaningsih Widagdo Sri Nugroho Woro Wulandari Kalanjati Wyanda Arnafia Yusuf Ridwan