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All Journal HAYATI Journal of Biosciences Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Bios Logos Jurnal Natur Indonesia Jurnal Forum Nuklir Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Hortikultura Jurnal AgroBiogen Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Sumberdaya Hayati BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education JURNAL PENDIDIKAN TAMBUSAI Jurnal Ilmiah Mandala Education (JIME) Jurnal Ilmu Kefarmasian Indonesia Amalee: Indonesian Journal of Community Research & Engagement Aphorisme: Journal of Arabic Language, Literature, and Education Informatics and Digital Expert (INDEX) NUSRA: Jurnal Penelitian dan Ilmu Pendidikan Jurnal Minfo Polgan (JMP) Indonesian Journal of EFL and Linguistics Jurnal Pusat Inovasi Masyarakat Formosa Journal of Science and Technology (FJST) Makara Journal of Science Eduvest - Journal of Universal Studies AL QALAM JURNAL KEPENDIDIKAN SINTESA Jurnal Intelek Insan Cendikia Reinwardtia Indonesian Journal of Innovation Multidisipliner Research Jurnal Ekonomi, Manajemen, Akuntansi Jurnal Pendidikan dan Sosial Humaniora Istifkar: Media Transformasi Pendidikan
Miftahudin .
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Bogor Agricultural University, Bogor
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Journal : Makara Journal of Science

 1 
The Molecular Diversity-based ISSR of Durio tanjungpurensis Originating from West Kalimantan, Indonesia Riupassa, Pieter Agusthinus; Chikmawati, Tatik; Miftahudin,; Suharsono,
Makara Journal of Science Vol. 19, No. 1
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The Durian Tengkurak (Durio tanjungpurensis Navia) is one of the endangered exotic species in the Malvaceae family. The species is important for conservation of the germplasm and is considered a potential genetic resource for the development of durian in the future. The objective of this research project was to assess the molecular diversity of D. tanjungpurensis in West Kalimantan, based on Inter Simple Sequence Repeat (ISSR) markers. We applied ten ISSR primers to reveal the genetic diversity of 60 individuals from six natural endemic D. tanjungpurensis populations. The genetic diversity parameters were estimated based on binary data about PCR products (present or absent bands). The results showed that the mean number of observed alleles, the mean number of effective alleles, the genetic diversity, the Shannon’s Information Index score, the number of polymorphic loci, and the percentage of polymorphic loci were 1.53, 1.29, 0.17, 0.26, 77.83, and 52.59, respectively. An analysis of molecular variance (AMOVA) showed that the genetic diversity within a population (65%) was higher than that found between the populations (35%). UPGMA clustering and principal coordinate analysis, based on the DICE similarity matrix, were used to classify the populations into three groups: 1) Hutan Rejunak and Tembaga, 2) Bukit Merindang, and 3) Hutan Rawak, Bukit Sagu 1, and Bukit Sagu 2. Further analysis of the population structure using STRUCTURE software was used to classify all the individuals into two major categories, thus uniting Groups 2 and 3 as one major category. In conclusion, a high level of genetic diversity in the Durian Tengkurak was revealed utilizing the ISSR markers employed in the study.
Phytochemical Composition of Selaginella spp. from Java Island Indonesia Chikmawati, Tatik; Setyawan, Ahmad Dwi; Miftahudin, Miftahudin
Makara Journal of Science Vol. 16, No. 2
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For a long time, people in West Java, Indonesia have used Selaginella as a traditional cure for several ailments including fever, minor wounds, broken bones, women’s health disorders or postnatal bleeding. However, information on the active compounds of the plant from Java Island has not been fully expounded. The objective of the research was to reveal the diversity of bioactive compounds and amentoflavone content of Selaginella from Java Island in order to optimize the use of this plant as a medicinal plant. Selaginella plants collected from 29 locations in Java were extracted and subjected to colorimetric and thin layer chromatography test to qualitatively analyze the bioactive compounds of alkaloid, phenol, and steroid. In addition, high performance liquid chromatography was performed to analyze the amentofalvone content of Selaginella extract. The research result showed that all the examined Selaginella species contained alkaloid, flavonoid, saponin, tannin, and steroid, but they did not contain hydroquinone. Seven of them contained the biflavonoid type of amentoflavone. The highest amentoflavone concentration, 6.87 ppm, was found in S. subalpina that originated from Gedung Songo, Central Java. Those results indicated that more than one Selaginella species originating from Java Island had marked potential for use as medicinal plants.
Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
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Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Co-Authors , Triadiati AD Ambarwati ADEEL ABDUL KARIM ALTUHAISH Afifah Azkiyah Ahmad Dwi Setyawan Ahmad Tarmidi Albarokah, Nurdiansyah ALEX HARTANA ALEX HARTANA Amarullah, Mohammad Syarifuddin Ambarwati, AD Anarki, Aprillia Alling Anjar Tri Wibowo Arafah, Purnama Aris Tjahjoleksono Atang Sutandi Awik Puji Dyah Nurhayati Aziz, Alvi Bachtiar, Machdum BUDI SETIADI DARYONO Budiarto, Despiyan Dwi CHAIDIR CHAIDIR Dasumiati Dasumiati Desvian Bandarsyah Devi Media Siska Dewi Indriyani Roslim DIANA AGUSTIN CAROLINA DIBYO PRONOWO Dwi Jatmoko Dwi Suci Setyaningsih DWINITA WIKAN UTAMI Dwinita Wikan Utami Eka Setiawan Fahrurrozi Fahrurrozi Faiz, Agus Maulana Fauzobihi, Fauzobihi Fuad Fitriawan, Fuad Habizar, Habizar Hafizhni Anggia, Rafi HAJRIAL ASWIDINNOOR Hamim Hamim Hanarida, I Harisca, Rodhi Hartanti, Fajri Hartati, N Sri Haryanti, Intisari HIMMAH RUSTIAMI Himmah Rustiami, Himmah I Hanarida I PUTU GEDE P. DAMAYANTO I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. I Rosdianti Ilham Dinar Akbar Cudino Iman Rusmana Iswandi Anas Isya, Mandanu Akhta Nur Iwan, Pristanto Ria Irawan J PERRY GUSTAFSON Joko Prasetiyono JULIARNI JULIARNI Juliyani Juliyani Kamini, Kamini Khoirurrijal Khoirurrijal, Khoirurrijal Komalasari, Selly Siti Komalasari Kustiariyah, Kustiariyah Kusumanegara, Kusumawaty LA KOLAKA Latifah, Vida Rahma M Ilham Fahmi Roji MARIA BINTANG Marlina Ardiyani Maududy, Robby Meitha, Karlia Moeljopawiro, Sugiono Muchozin, Muhammad Ni'an Muhammad Akbar Velayati Muhammad Alfarabi Mukhlash Abrar Munif Ghulamahdi Muslimin Muslimin MUTTAQIN, MAFRIKHUL Nadzar Saifudin Nasir, Fahad Muhammad Nasir Nettyani, Naipospos Nugroho, Giri Nunung Fajaryani, Nunung Nurlaela Nurlaela Nurlaela Nurlaela Nurlaila, Santi Nurlaila OKKY SETYAWATI DHARMAPUTRA Pieter Agusthinus Riupassa Polosoro, Aqwin Prasetiyono, Joko Purwanti, Tira Puspita Dewi Sopyati Putri Aulia Putro, Karso Suryo Raden Mohamad Herdian Bhakti RANTJE LILLY WORANG Rasyid, Aliy Ratnasari, Seli Risa Aryantri Rizal Nursamsi, Dede RIZAL SYARIEF Rosandi Ardi Noegraha Rosdianti, I Ruwirohi, Jan Everhard S Yuriyah Sahab, Fahmi Sahab Sairun Sairun Salimah, Hafshah Aqidatun Sandra Arifin Aziz Sartika, Dian Setiawan Setiawan Setiawan, Jeffry SISWA SETYAHADI Siti Maryam Harahap Slamet, Oky Sobir Sobir Sokoy, Mercy Sopandi, Nur Irfan Sri Ernawati Srifariyati, Srifariyati Sudirman Yahya Sugeng Heri Suseno Sugiono Moeljopawiro Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono, Suharyadi Suharyadi Sulistiani, Erina Supena, Ence Darmo Jaya Sutiarso Sutiarso Suwaji Handaru Wardoyo Suwarno Suwarno Suwarno Suwarno TATIK CHIKMAWATI Tnunay, Ite Morina Yostianti Toto Hadiarto Trikoesoemaningtyas Turhadi Turhadi Utut Suharsono Utut Widyastuti Violita Violita Wardhani, Hilda Sari Wening Enggarini Windarti Wahyuningtyas Windriyana, Windriyana Yuriyah, S Yusrian, Afif Ramdhan Yusuf, Adib Fakhruddin Yuyun Rohmatul Uyuni Zurtiana, Ahmad