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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Pharmaciana: Jurnal Kefarmasian Biota Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Indonesian Journal of Chemistry Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Jurnal Mandala Pharmacon Indonesia Dinamika Journal : Pengabdian Masyarakat Narra J INDRA: Jurnal Pengabdian kepada Masyarakat Jurnal Farmasi Indonesia
Firzan Nainu
Fakultas Farmasi, Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Journal : Jurnal Veteriner

 1 
Antibiotic Sensitivity Pattern of Staphylococcus aureus and Escherichia coli Isolated from Bovine Fresh Milk (POLA SENSITIVITAS ANTIBIOTIK TERHADAP STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI YANG DIISOLASI DARI SUSU SAPI SEGAR) Lucia Ratna Winata Muslimin; Firzan Nainu; Rochmat Himawan
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this study were to determine the sensitivity of S.aureus and E. coli isolated fromfresh milk against against several antibiotics and to determine the safety of the milk for humancomsumsion. Milk was collected from milking diary cow and was used for the bacterial isolation. E.coli were were identified using Total Plate Count (TPC), Gram staining, their growth on Endo Agarand Eosin MethyleneBlue Agar, Biochemical analysis including glucose, lactose, sucrose,maltose, andsorbitol would be followed by Sorbitol Mac Conkey Agar Test for the identification of E.coliO157:H7.The isolation and identification of S.aureus were performed using Gram stain, TPC, growth on BairdParker Agar and Mannitol Salt Agar. S. aureus and S. epidermidis were differentiated by coagulaseand catalase tests. The antibiotic sensitivity tests for both S. aureus and E.coli were carried out usingthe following antibiotics: ampicillin, bacitracin, vancomycin, cefoperazone, ceftriaxone, cefotamine,cefuroxime, cefepime, cefazoline, ceftazidime, chloramphenicol, tetracycline, doxycycline, amikacin,kanamycin, neomycin, ertapenem, meropenem, imipenem, erythromycin, gentamycin, nalidixic acid,ciprofloxacin, levofloxacine, norfloxacine, ofloxacin, and novobiocin. Fresh milk obtained from thefarm was positive for S.aureus and E.coli and resistant to most antibiotics tested. The best antibioticsfor S. aureus were imipenem (54.1 mm), ampicillin (42.3 mm), cefazolin (41.6 mm), doxycycline (41.15mm), and for E.coli were Imipenem (30.1 mm), ertapenem (29.5 mm), and meropenem (25.35 mm). Thebovine fresh milk examined was contaminated by S.aureus and E.coli and to some extent, were alsoresistant to most antibiotics tested.
Co-Authors Aamer Ikram Abdeen, Ziad Acharya, Yogesh Ahmad Mu'arif Ahmad Najib Ai Koyanagi Aksa, Rahmad Amanda Yufika Amir, Muhammad Nur Amirullah -, Amirullah Anak Agung Dewi Megawati, Anak Agung Dewi Anastasia Bondarenko Andi Maghfirah Nurul Fitri Andri Frediansyah Andri Frediansyah, Andri Arba, Muhammad Arsyik Ibrahim As'ad, Muhammad F. Asbah, Asbah Asri, Rangga M. As’ad, Muh. Fadhil Bahar, Muhammad A. Bapulo, Nurdewi N. Base, Nurul Hidayah Bob Wilffert Bohari Yusuf Bondarenko, Anastasia Chadran, Deepak Chaeratunnisa, Rizkya Chandran, Deepak Deepak Chandran Dewi Megawati Dewi Purwaningsih Dewi Yuliana Dewita Fatiah Dina E. Sallam Dini Ayu Ariastiwi B Djide, Nana Juniarti Natsir Elly Wahyudin Emil Salim Emran, Talha B. Endang Mutiawati Rahayuningsih* ENDANG MUTIAWATI, ENDANG Fahriani, Marhami Fajar, Jhonny K. Fathima, Raisha Fatmawati Fatmawati Fauzi Yusuf Fauzi Yusuf, Fauzi Filmaharani, Filmaharani Francesco Rosiello Gemini Alam Habibie Habibie HANIFAH YUSUF Hanifah Yusuf Harapan Harapan Harapan Harapan Harapan Harapan Hardiyanti, Widya Hassan , Wardah Herlina Rante Herlina Rante Herman Herman Hibban Ar Royan Husnah, Milda I Made W. Wardhana Ikram Ikram Ikram, Aamer Ilmawan, Muhammad Iqhrammullah, Muhammad Irfan Ullah Islamudin Ahmad Ismail Ismail J. Smith Torres-Roman James-Paul Kretchy Jhonny K. Fajar Jonny K. Fajar Katarzyna Kotfis Kazmi, Syeda K. Keam, Synat Khader, Yousef Khaerani, Mufliha Khairuddin - Khan Sharun Kidoura, Yuto Kotfis, Katarzyna Koyanagi, Ai Kretchy, James-Paul Kurnia F. Jamil Kurnia F. Jamil Laode Rijai Latada, Nadila P. Latada, Nadila Pratiwi Latief, Kamaluddin Latifah Rahman Lee Smith Lemu, Yohannes K. Lucia Ratna Winata Muslimin M. Natsir Djide M. Natsir Djide Maelani, Imelda Maliga, Helnida A. Malik Sallam Mamada, Sukamto Mamada, Sukamto S. Mangilep, Muhammad Agung Ady Marhami Fahriani Marianti A Manggau Marianti A.Manggau Marius Rademaker Masyeni, Sri Mellinia, Sania A. Memish, Ziad A. Milda Husnah Mirhansyah Ardana Mu'arif, Ahmad Mudatsir Mudatsir Mudjahid, Mukarram Mufidah Murdifin Muh. Akbar Bahar Muhammad Ahsan Muhammad Ilmawan Muhammad J. Tahir Muhammad Natsir Djide Mutiara, Suci Nakanishi, Yoshinobu Nalapraya, Widhy Y. Nanda, Cut M. Nirwana, Aura Nur Rahma Rumata Ophinni, Youdiil Pratama, Muhammad R. Putri, Annisa A. Putri, Tenri ZAD. Rademaker, Marius Rangga Meidianto Asri Rezky Yanuarti Risfah Yulianty Rochmat Himawan Rosa, Reski A. Rosa, Reski Amalia Rosa, Sandro GV. Rosany tayeb Rosiello, Francesco Royan, Hibban Ar Rudi Arfiansyah Rudi Arfiansyah Rudi Arfiansyah Rudi Arfiansyah sakiya syahrir sudirman Salin Sirinam Sallam, Dina E. Sallam, Malik SALSABILA, ANNISA Samsul Anwar Sandro GV. Rosa Sandro Vento Sari, Dwi K. Sarifuddin Sarifuddin Sartini Sartini Sartini Sartini Selvi Jumiatul Astati Sharun, Khan Shiratsuchi, Akiko Sirinam, Salin Sitti Hadijah Sitti Nurhamidah Smith, Lee Subehan Subehan Subehan, Subehan Suhenro, Suhenro Sukamto Mamada Sukamto S. Mamada Syahraini, Aigia Syamsu Nur, Syamsu Syeda K. Kazmi Synat Keam Tahir, Muhammad J. Takayuki Kuraishi Talha B. Emran Toriba, Akira Torres-Roman, J. Smith Tri Puspita Roska Tri Puspita Roska Ullah , Irfan Umar, Abdul H. Usmar Usmar Usmar Usmar Vento, Sandro Wardah Hassan Yesi Astri Yogesh Acharya Yohannes K. Lemu Youdiil Ophinni Yousef Khader Yufika, Amanda Yulia Yusrini Djabir Ziad A. Memish Ziad Abdeen Zubair, Muhammad S.