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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Pharmaciana: Jurnal Kefarmasian Biota Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Indonesian Journal of Chemistry Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Jurnal Mandala Pharmacon Indonesia Dinamika Journal : Pengabdian Masyarakat Narra J INDRA: Jurnal Pengabdian kepada Masyarakat Jurnal Farmasi Indonesia
Firzan Nainu
Fakultas Farmasi, Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Journal : Biota

 1 
Determination of Genetic Mutation Profile of drpr Gene in Drosophila melanogaster using High-Resolution Melting Analysis Ahmad Mu'arif; Rudi Arfiansyah; Tri Puspita Roska; Herlina Rante; Firzan Nainu
Biota Vol 8 No 1 (2022): Jurnal Biota 2022
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v8i1.7556

Abstract

Genotype determination of experimental animals is generally conducted using sequencing methods that require expensive cost as well as experience and special equipment. This study aimed to determine the presence of drpr gene mutation in Drosophila melanogaster using coupled real time PCR-High Resolution Melting (real time PCR-HRM) as an alternative method. Two types of fly samples, w1118 and drprΔ5 were used as wildtype control and mutant genotype, respectively. The DNA from twenty of each w1118 and mutant drprΔ5 flies were isolated and amplified using real time PCR. The generated amplicons were then further processed by HRM method at the temperature of 60-95°C. This study demonstrated that the real time PCR-HRM method could distinguish wildtype control w1118 and mutant drprΔ5 based on the HRM data with the confidence level was more than 90%. Therefore, this study provides an evidence that real time PCR-HRM method might be beneficial to screen the mutant genotype from its wildtype counterpart based on differences in the melting temperatures due to changes at nucleotide base level
Co-Authors Aamer Ikram Abdeen, Ziad Acharya, Yogesh Ahmad Mu'arif Ahmad Najib Ai Koyanagi Aksa, Rahmad Amanda Yufika Amir, Muhammad Nur Amirullah -, Amirullah Anak Agung Dewi Megawati, Anak Agung Dewi Anastasia Bondarenko Andi Maghfirah Nurul Fitri Andri Frediansyah Andri Frediansyah, Andri Arba, Muhammad Arsyik Ibrahim As'ad, Muhammad F. Asbah, Asbah Asri, Rangga M. As’ad, Muh. Fadhil Bahar, Muhammad A. Bapulo, Nurdewi N. Base, Nurul Hidayah Bob Wilffert Bohari Yusuf Bondarenko, Anastasia Chadran, Deepak Chaeratunnisa, Rizkya Chandran, Deepak Deepak Chandran Dewi Megawati Dewi Purwaningsih Dewi Yuliana Dewita Fatiah Dina E. Sallam Dini Ayu Ariastiwi B Djide, Nana Juniarti Natsir Elly Wahyudin Emil Salim Emran, Talha B. Endang Mutiawati Rahayuningsih* ENDANG MUTIAWATI, ENDANG Fahriani, Marhami Fajar, Jhonny K. Fathima, Raisha Fatmawati Fatmawati Fauzi Yusuf Fauzi Yusuf, Fauzi Filmaharani, Filmaharani Francesco Rosiello Gemini Alam Habibie Habibie HANIFAH YUSUF Hanifah Yusuf Harapan Harapan Harapan Harapan Harapan Harapan Hardiyanti, Widya Hassan , Wardah Herlina Rante Herlina Rante Herman Herman Hibban Ar Royan Husnah, Milda I Made W. Wardhana Ikram Ikram Ikram, Aamer Ilmawan, Muhammad Iqhrammullah, Muhammad Irfan Ullah Islamudin Ahmad Ismail Ismail J. Smith Torres-Roman James-Paul Kretchy Jhonny K. Fajar Jonny K. Fajar Katarzyna Kotfis Kazmi, Syeda K. Keam, Synat Khader, Yousef Khaerani, Mufliha Khairuddin - Khan Sharun Kidoura, Yuto Kotfis, Katarzyna Koyanagi, Ai Kretchy, James-Paul Kurnia F. Jamil Kurnia F. Jamil Laode Rijai Latada, Nadila P. Latada, Nadila Pratiwi Latief, Kamaluddin Latifah Rahman Lee Smith Lemu, Yohannes K. Lucia Ratna Winata Muslimin M. Natsir Djide M. Natsir Djide Maelani, Imelda Maliga, Helnida A. Malik Sallam Mamada, Sukamto Mamada, Sukamto S. Mangilep, Muhammad Agung Ady Marhami Fahriani Marianti A Manggau Marianti A.Manggau Marius Rademaker Masyeni, Sri Mellinia, Sania A. Memish, Ziad A. Milda Husnah Mirhansyah Ardana Mu'arif, Ahmad Mudatsir Mudatsir Mudjahid, Mukarram Mufidah Murdifin Muh. Akbar Bahar Muhammad Ahsan Muhammad Ilmawan Muhammad J. Tahir Muhammad Natsir Djide Mutiara, Suci Nakanishi, Yoshinobu Nalapraya, Widhy Y. Nanda, Cut M. Nirwana, Aura Nur Rahma Rumata Ophinni, Youdiil Pratama, Muhammad R. Putri, Annisa A. Putri, Tenri ZAD. Rademaker, Marius Rangga Meidianto Asri Rezky Yanuarti Risfah Yulianty Rochmat Himawan Rosa, Reski A. Rosa, Reski Amalia Rosa, Sandro GV. Rosany tayeb Rosiello, Francesco Royan, Hibban Ar Rudi Arfiansyah Rudi Arfiansyah Rudi Arfiansyah Rudi Arfiansyah sakiya syahrir sudirman Salin Sirinam Sallam, Dina E. Sallam, Malik SALSABILA, ANNISA Samsul Anwar Sandro GV. Rosa Sandro Vento Sari, Dwi K. Sarifuddin Sarifuddin Sartini Sartini Sartini Sartini Selvi Jumiatul Astati Sharun, Khan Shiratsuchi, Akiko Sirinam, Salin Sitti Hadijah Sitti Nurhamidah Smith, Lee Subehan Subehan Subehan, Subehan Suhenro, Suhenro Sukamto Mamada Sukamto S. Mamada Syahraini, Aigia Syamsu Nur, Syamsu Syeda K. Kazmi Synat Keam Tahir, Muhammad J. Takayuki Kuraishi Talha B. Emran Toriba, Akira Torres-Roman, J. Smith Tri Puspita Roska Tri Puspita Roska Ullah , Irfan Umar, Abdul H. Usmar Usmar Usmar Usmar Vento, Sandro Wardah Hassan Yesi Astri Yogesh Acharya Yohannes K. Lemu Youdiil Ophinni Yousef Khader Yufika, Amanda Yulia Yusrini Djabir Ziad A. Memish Ziad Abdeen Zubair, Muhammad S.