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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Berkala Ilmu Kesehatan Kulit dan Kelamin Indonesian Journal of Tropical and Infectious Disease ISM (Intisari Sains Medis) : Jurnal Kedokteran JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal EduHealth Molucca Medica
Linda Astari, Linda
Departemen/Staf Medik Fungsional Ilmu Kesehatan Kulit Dan Kelamin Fakultas Kedokteran Universitas Airlangga/Rumah Sakit Umum Daerah Dr. Soetomo Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
Co-Authors Afif Nurul Hidayati Afif Nurul Hidayati, Afif Nurul Alarik L., Albertus Alimsardjono, Lindawati Alpha Fardah Athiyyah Alya Nuha Andini Amanda Gracia Manuputty Anang Endaryanto Anggraeni Noviandini Anggraeni, Sylvia Ardhiah Iswanda Putri Arifa Mustika Astindari Astindari Astindari Astindari, Astindari Aulia, Syifa Auliya, Arsya Axelia, Presstisa Gifta Bagus Bagus Bagus Haryo Kusumaputra, Bagus Haryo Bramantono Bramantono, Bramantono Brigita Ika Rosdiana Budi Prasetyo Budi Utomo Budi Utomo Budiono Budiono Chesia Christiani Liuwan Christina Avanti Cita Rosita Sigit Prakoeswa Damayanti Damayanti Damayanti Damayanti Damayanti Damayanti Damayanti David Sontani Perdanakusuma Densy Violina Harnanti Desiana Widityaning Sari Dewi Puspitorini Dhyah Aksarani Handamari Diah Mira Indramaya DINAR ADRIATY Dwi Murtiastutik Dwi Ratna Adisty Dwiyanti Puspitasari, Dwiyanti Dyah Ratri Anggarini Dyah Ratri Anggarini, Dyah Ratri Endang Wahyu Fitriani Erwin Astha Triyono Esti Hendradi Eva Lydiawati Evy Ervianti Fadila, Arisia Farhat Surya Ningrat Hadiwidjaja, Farsha Naufal Hari Sukanto, Hari Harningtyas, Citra Dwi Hartanto, Felix I Gusti Ayu Agung Elis Indira I Gusti Nyoman Darmaputra Indah Purnamasari Indiastuti, Danti Nur INDROPO AGUSNI Ingrid Suryanti Suryono Irmadita Citrashanty Irmadita Citrashanty, Irmadita Iskandar Zulkarnain Iskandar Zulkarnain ISWAHYUDI ISWAHYUDI Kathrin Kezia Henry Kinanti Prabawaningrum Kurniati Kurniati M. Yulianto Listiawan ManikRetno Wahyunitisari Mappamasing, Hasnikmah Maylita Sari Maylita Sari, Maylita Medhi Denisa Medhi Denisa Alinda, Medhi Denisa Meita Ardini Pratamasari, Meita Ardini Merilyne Merilyne Musofa Rusli Ngesti Kumalasari, Diah Noviannisa, Farah Ayu Nyoman Suryawati Pepy D. Endraswari Pepy Dwi Endraswari, Pepy Dwi Prakoeswa, Cita Rosita Priangga Adi Wiratama Puspowati, Erindah Putri Hendria Wardhani Putri, Melati Adinda Rahmadewi Rahmadewi Rahmadewi Rahmatari, Bandaru RATNA WAHYUNI Regitta Indira Agusni Renata Prameswari Retha Retha Ridha Ramadina Widiatma Riyana Noor Oktaviyanti Sawitri Sawitri Sawitri Selvia Yuliani Dwi Ratih Sanggarwati Sepling Paling Sheilaadji, Maria Ulfa Shinta Dewi Rahmadhani Soetojo SHINZO IZUMI Siti Kurniawati Sulaksanaswastho Suyoso Sunarso Suyoso Sunarso Suyoso Sunarso Suyoso Sunarto, Olivia Awwalin Susanto, Ester Chateline Tjokorde Istri Nyndia Vaniari Trisniartami Setyaningrum Vidyani Adiningtyas Widyantari, Septiana Willy Sandhika Yuri Widia, Yuri Yuri Widya Zahruddin Ahmad