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All Journal Agrovigor: Jurnal Agroekoteknologi Biotropika Jurnal Agroekoteknologi Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat BERITA BIOLOGI Jurnal Ilmiah Pertanian BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biologi Tropis Agrisocionomics: Jurnal Sosial Ekonomi Pertanian Jurnal Masyarakat Merdeka (JMM) Jurnal Ilmiah Hijau Cendekia MONSU'ANI TANO Jurnal Pengabdian Masyarakat JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Bubungan Tinggi: Jurnal Pengabdian Masyarakat Journal of Community Service and Empowerment Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Agrifarm Jurnal Agroteknologi Agrivet: Jurnal Ilmu-ilmu Pertanian dan Peternakan J-ABDIPAMAS (Jurnal Pengabdian Kepada Masyarakat) JATIMAS : Jurnal Pertanian dan Pengabdian Masyarakat Jurnal Ilmiah Pertanian Nasional
Nugraheni Hadiyanti
Universitas Kadiri
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Physics Public Health Social Sciences Other

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Journal : Biotropika

 1 
Identification and Genetic Diversity of Pineapple Local Accessions from Kediri and Closely Related Species (Bromeliaceae) Based on matK Gene Rasyadan Taufiq Probojati; Hadiyanti, Nugraheni; Hapsari, Lia
Biotropika: Journal of Tropical Biology Vol. 12 No. 3 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.03.01

Abstract

Ananas comosus L. belongs to the Bromeliaceae family and has around eight subfamilies. Accessions of local pineapple have spread widely in several areas, one of which is in Kediri. However, some accessions and closely related species (Bromeliaceae) likely remain poorly understood relationships. The highly diverse morphological characters and low levels of sequence divergence in Bromeliaceae have been problematic in resolving the reconstruction of phylogenetic relationships. This study aimed to evaluate the efficiency of the matK gene as a DNA barcode to determine genetic diversity and reconstruct a phylogenetic tree in local pineapple and closely related species (Bromeliaceae). A total of 15 specimens were used in this research, of which 7 were local pineapple accessions from Kediri and eight were closely related species from the Bromeliaceae family. Whole genome DNA was isolated using a Tiangen kit and amplified using the polymerase chain reaction (PCR) technique using a specific primer. The results showed that matK was easily amplified with a DNA fragment length of 750-800 bp. The average composition value of each base was 36.9% T (U) bases, 30.1% in A bases, 15.9% in G bases, and 17.1% in C. BLAST analysis of the sequences showed that all samples were confirmed and identified according to the species names spread across local pineapple production areas (with query cover of 96-98%). However, the species names registered in the PBG database were confirmed to be different species but still in the same genus, i.e., samples B9-B14. The phylogenetic relationship analysis distinguished each accession into two large clades according to sub-families, i.e., Bromelioideae and Pitcairnioideae. This research shows that the matK gene is suitable and recommended as a DNA barcode for local pineapple and closely related species.
Co-Authors Achmad Yogi Pambudi ADAM MAHARDIKA Agustia Dwi Pamujiati Ahmad Haris Hasanuddin Slamet Anak Agung Gede Sugianthara Andreas Zulkarnain Anindita, Devina Cinantya Anshor, Yoyok Zakaria Arissaryadin, Arissaryadin Avisema Sigit Saputro Ayuningtyas, Nanda Widyawati Azkiyah, Lailatul Bambang Dwi Moeljanto Chamro', Wardatul Dektiyansyah Nusantara Sukoco Devina Cinantya Anindita Dewi, Rifani Rusiana Dian Wahyudi Dodik Eka Setiawan Dona Wahyuning Laily Edy Kustiani Eko Eko, Eko Eko Hariyanto Eko Yuliarsha Sidhi Firdausi, Muhammad Riza Ginji Liani HARIYANTO HARIYANTO Hendrik Setyobudi Ikawati, Haryanti Insiyah, Bindari Junaidi Junaidi Junaidi Junaidi Junaidi Junaidi Junaidi Kholik, Dian Abdul Lia Hapsari Mahardhika, Adam Mariyono Mariyono Mariyono Mariyono Maryono Moch. Agus Suryo Wibowo Mufiana Alfatin muharram, Muhammad Nada Anesya Nanang Aji Saputro Nareswari, Aptika Hana Prastiwi Nina Lisanty Nixie Azalia Whintisna Nur Ulfa Turohmah Nur Ulfa Turrohmah Nurcahyo, Agung Wilis Pardono . Pardono Pardono Pardono Pardono Pratama, Dandy Pramizza Adi Prayoga, Reksa Nanda Rachmad Chairul Huda Rafelda Dias Nurfitri Ramadhan, Riski Jefri Rasyadan Taufiq Probojati Risma Ari Prayitno Ryan Edy Saputra Saptorini Saptorini Saptorini Saptorini Saptorini, Saptorini Saputra, Yudha Saputro, Avisema Sigit Satriya Bayu Aji Setyobudi, Hendrik Subarkah, Minal Kholik Prima Suherman Suherman SUNARTO Supandji Supandji Supandji Supandji Supandji, Supandji Supramono, Agus Supriyadi Supriyadi Supriyadi Supriyadi Tafakresnanto, Chendy Tjatur Prijo Rahardjo Tjatur Prijo Rahardjo Turohmah, Nur Ulfa Virgian Galuh Agusty Wahyu Widiyono Widi Artini Windy Silvyana WIWIEK ANDAJANI Yuliana, Luluk Yuliyanto Yuliyanto