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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Buletin Hama dan Penyakit Tumbuhan JURNAL KIMIA SAINS DAN APLIKASI Prosiding Seminar Biologi BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Sumberdaya Hayati Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA ZOO INDONESIA JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Pembelajaran dan Biologi Nukleus Zoo Indonesia International Journal of Social Service and Research Makara Journal of Science Policy Brief Pertanian, Kelautan, dan Biosains Tropika

RIKA RAFFIUDIN

Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University. Jl. Agatis, Kampus Dramaga, Bogor 16680, Indonesia

Author-ID : 354264

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary Other

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Antioxidant and Antibacterial Activity of Selected Indonesian Honey against Bacteria of Acne Shafira Ananda Djakaria; Irmanida Batubara; Rika Raffiudin
Jurnal Kimia Sains dan Aplikasi Vol 23, No 8 (2020): Volume 23 Issue 8 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Honey has natural bacteriostatic and bactericide activities; thus, it could be active compounds as an antibacterial against Propionibacterium acnes, the main bacteria in acne forming state. Our study aims to determine phenolic and flavonoid contents (qualitative and quantitative), antioxidants, along with antibacterial activity against P. acnes of selected Indonesian honey. Honey used in this study, including honeybees and stingless bees, were collected from Sumbawa, Riau, Belitung, Sukabumi, Banyuwangi, Bogor, and South Sulawesi. In the method, the quantitative content of phenols and flavonoid were measured using a spectrophotometer. Further, DPPH (2, 2’-diphenylpicryl hydrazyl) and ABTS (2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) assays were used to measure the antioxidant activities, and subsequently microdilution method was applied to determine antibacterial activity. The results showed that Indonesian honey contains phenolics and flavonoids in the range of 49.5-67.3 μg Gallic Acid Equivalent (GAE)/g and 766.5-1509.5 μg Quercetin Equivalent (QE)/g, respectively. All honey samples also show antioxidant and antibacterial activities. Of note, Apis cerana honey from Banyuwangi has the highest antioxidant and antibacterial activity compared to others. It has the Inhibition Concentration (IC50), Trolox Equivalent Antioxidant Capacity, Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC) values of 59.85 mg/L, 122 μg Trolox/g, 125 mg/mL, and 500 mg/mL respectively. Our study reveals that Indonesian honey can inhibit and kill P. acnes as well. Nonetheless, the phenolic content of honey is not always linear with antioxidant and antibacterial activities.

Characterization of Exon and Intron of Defensin 1 Gene in Apis cerana and Apis dorsata Alfiyan, Achmad; Raffiudin, Rika; Astuti, Rika Indri
Makara Journal of Science Vol. 23, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Honey bee defensin 1 gene belongs to the class of immunity genes in this social insect. The peptide acts as a defensive mechanism against infections caused by Gram-positive bacteria. The aim of this study was to characterize exon 2, intron 2, and exon 3 of defensin 1 gene in both the Indonesian honey bees Apis cerana and A. dorsata. First, defensin 1 genes of A. cerana and A. dorsata were sequenced, after which a bioinformatic analysis was conducted. The amplified length of these defensin 1 genes of A. cerana and A. dorsata were 479 and 458 bp, respectively, and their putative amino acid sequences comprised 66 and 65 amino acids, respectively, with 6 cysteine residues. The cysteine residues formed a disulfide bond and then linked the three domains in the defensin peptide with each other, thereby allowing the lysis of the bacterial membrane through pore formation. Intron 2 of the defensin gene demonstrated nucleotide variations between A. cerana from Indonesia and that from Korea and between A. dorsata from Indonesia and that from Malaysia; the latter species also demonstrated variations in exon 3. Phylogenetic tree topology of the bee, which was constructed based on defensin 1 gene, was compatible with a previous study showing that A. cerana and A. dorsata are more closely related to A. mellifera than to A. florea.

The Effectiveness of Stingless Bees on Pollination of Bitter Melon Plants Momordica charantia L. (Cucurbitaceae) Andi Gita Maulidyah Indraswari Suhri; RC Hidayat Soesilohadi; Ramadhani Eka Putra; Rika Raffiudin; Hery Purnobasuki; Ali Agus; Sih Kahono
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69124

This study aimed to measure the effectiveness of stingless bee Tetragonula cf. biroi pollination on the fruit formation of bitter melon Momordica charantia plants. We used hoods on the observed bitter melon plants. In the first hood, stingless bees are inserted to help pollinate 100 bitter melon plants, while in the other hoods, stingless bees are not inserted so that there is no assistance in pollinating the other 100 bitter melon plants. The method used is the focal sampling method for 25 days of observation. Based on the results of the study, stingless bee pollination assistance increased the percentage of the number of flowers that became fruit by 390%, the weight of seeds/fruit by 64%, number of seeds/fruit by 260%, fruit weight by 163%, fruit diameter by 91%, and fruit length by 86%. In addition to the size of the fruit, the shape of the bitter melon pollinated by bees is standard (long and straight). In contrast, the bitter melon that does not get pollination assistance grows with a bent shape resembling the letter "C." Bitter melon is an agricultural commodity that needs pollinating agents such as stingless bees because of its monoecy.

Pemodelan Habitat Potensial Tumbuhan Lebah Apis dorsata di Membalong, Belitung Muhammad Basrowi; Ibnul Qayim; Rika Raffiudin
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 4 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.4.562

The forest becomes a habitat for honey bees to carry capacity for the supply of feed sources. Apis dorsata requires the availability of plants for supplying nectar and pollen. Traditional system in Belitung uses rafters for the efficiency of honey harvesting. Studies about preference habitats for bee forage become important to perform conservation of the honey bees. Therefore, the objective of this study was to (1) identify the composition, structure distribution patterns and vegetation profiles, (2) identify the area of the appropriate habitat model and environmental variables that influence the carrying capacity to provide bee forage of A. dorsata. Purposive sampling was used to analyze vegetation structure, distribution patterns, and vegetation profiles. The determination of the distribution of species is obtained from the Morishita index. We used MaxEnt for species distribution modeling to predict the potential plant habitat of bee plants. Our results showed that five most highest-importance plants. Myrtaceae is a family with dominant compositions and INP such as Melaleuca cajuputi, Tristaniopsis obovata, and Eugenia leptantha. The structure of other families such as Theaceae (Schima wallichii), and Clusiaceae (Calophyllum pulcherrimum), are the species that have the highest importance. The pattern of distribution of bee forage is clumped. The canopy cover around the honeycomb area tends to be relatively open. Based on the modeling habitat, categories with very appropriate for the presence of bee forage not spread throughout Membalong Regency with a percentage of the area (4.86%) and in the buffer zone (10.53%), environmental variables that influence the distribution are temperature seasonality, maximum temperature warmest month, slope, precipitation on warmest quarter and soil ph. Keywords: habitat modeling, rafter, spatial distribution, vegetation analysis, vegetation profiles

Melissopalynology and Vegetation Analysis Surrounding Sunggau of Giant Honey Bee Apis dorsata in Belitung Regency Dwika Bramasta; Ibnul Qayim; Nina Ratna Djuita; Rika Raffiudin; Ramadhani Eka Putra; RC Hidayat Soesilohadi; Hery Purnobasuki
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1167-1174

The forest conversion into oil palm plantations in Belitung impacts the plant source for pollen and nectar to support the honey bee Apis dorsata. This study aimed to identify the plants used by A. dorsata as pollen sources in honey and bee bread in honey bee nests and to analyze the vegetation composition and structure surrounding the sunggau (artificial nesting site) in Belitung Regency. Honey from A. dorsata was collected from bee nests in sunggau on Kampak Island and Tanjung Rusa. The pollen grains from 20 ml honey were acetolysed and counted until 1,200 grains for each honey sample. In Kampak Island, we found eight pollen types in honey dominated by Rhizophora mucronate mangrove pollen and eleven pollen types in bee bread dominated by Melaleuca cajuputi. The pollen type in honey in Tanjung Rusa was similar to those in Kampak Island, and nine pollen types were found in the bee bread dominated by Elaeis guineensis. The vegetation analysis revealed that mangrove and heath forests in Kampak Island were dominated by Lumnitzera littorea and Melaleuca cajuputi, respectively. The results of this study confirm the bees' notable use of the mangrove ecosystem, which adds conservation value, especially in supporting bee management efforts in Belitung.

Variasi perilaku mencari makan pada semut rangrang Oecophylla smaragdina (Fabricius) pada habitat yang berbeda: Variations of foraging behavior of weaver ants Oecophylla smaragdina (Fabricius) in different habitats Rezki, Rezki; Aoliya, Nur; Fadliansyah, Fadliansyah; Wulandari, Siti Latifa; Jesajas, David Reinhard; Raffiudin, Rika
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.141

Weaver ants (Oecophylla smaragdina (Fabricus)) are carnivorous insects and play a role as biocontrol agents in urban habitats. This study aims to analyze the foraging behavior of O. smaragdina in two different habitats, namely urban and non-urban. The two locations are characterized by differences in the intensity of human interaction and the distance between the trees and the tree canopy. The behavioral observation method uses all sampling events with five behavioral categories: lurking (M1), approaching (M2), carrying (M3), communicating between ants in a colony (K1), and competition with other ants (K2). We tested three different types of feed: chick scraps, Tenebrio molitor Linnaeus larvae, and sugar. The results showed that there were three sequences of foraging behavior in urban areas, namely: (1) M1, M2, K1, M3, (2) M1, M2, K1, M3, K2, and (3) M1, M2, K2, M3. Meanwhile, there is only one pattern in non-urban areas, namely M1, M2, M3. Approaching food (M2) and lurking food (M1) were the dominant responses of O. smaragdina in urban and non-urban areas. In urban areas, broiler chickens were the type of feed most approached by ants, followed by sugar and beetle larvae. O. smaragdina in non-urban areas also preferred chicken pieces as feed compared to the others, but only one individual O. smaragdina approached (M2) chicken pieces and none approached the others. Foraging ants are more active at high temperature and low humidity, compared to low temperature and high humidity. Therefore, the foraging behavior of O. smaragdina is influenced by habitat factors.

The characterization of Apis cerana and Apis mellifera mrjp2 gene in Indonesia and the phylogeny relationship with mrjp family genes: Karakterisasi gen mrjp2 Apis cerana dan Apis mellifera di Indonesia dan hubungan filogeni dengan gen mrjp famili Shullia, Nurul Insani; Febiriani, Tia Vina; Raffiudin, Rika; Widjaja, Candra; Atmowidi, Tri
Jurnal Entomologi Indonesia Vol 21 No 1 (2024): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.1.1

The major royal jelly protein 2 (mrjp2) gene is one of the molecular markers that can discriminate between Apis cerana Fabricius and A. mellifera Linnaeus. Due to the lack of mrjp2 gene sequences registered in GenBank for Indonesian A. cerana and A. mellifera, DNA characterization and bioinformatics analysis were needed. This research aimed to characterize the exon-intron organization of mrjp2 genes for both Indonesian bee species and analyze the phylogenetic relationship with other mrjp family genes. DNA samples of Apis cerana and A. mellifera, collected from Bogor, were amplified using MF-MR primer at annealing temperatures of 47 oC and 50 oC, respectively. The length of A. cerana and A. mellifera DNA sequences were 579 and 597 bp, respectively. The DNA sequences of both species were comprised of partial exons 1, introns 1, exons 2, introns 2, and partial exon 3. The number of putative amino acids of A. cerana and A. mellifera mrjp2 genes were 111 and 123, respectively. We confirmed that the partial MRJP2 putative amino acids of both honey bees belong to the MRJP family and contained the peptide signal in the 14 first amino acid sites. Nucleotide variation of the mrjp2 gene in A. cerana was higher than A. mellifera. Phylogenetic tree construction showed that A. cerana and A. mellifera form a monophyletic clade with the A. mellifera mrjp7 gene and another mrjp family gene clustered as reported in a previous study.

Evaluation of Honey Production for Industry Purposes, The Case of Beekeeping with Apis Mellifera in Indonesia After The Covid-19 Pandemic Purwanto, Didik B.; Kuntadi, Kuntadi; Adalina, Yelin; Meilin, Araz; Raffiudin, Rika; Sahlan, Muhamad
International Journal of Social Service and Research Vol. 4 No. 03 (2024): International Journal of Social Service and Research (IJSSR)
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/ijssr.v4i03.719

The Indonesian beekeeping industry, grappling with diverse bee species, focuses on the sustainability of operations, particularly with the introduction of Apis mellifera during the early stages of the pandemic. This study explores post-pandemic Apis mellifera honey production in Indonesia, analyzing its impact on prices and business sustainability. Surveys and interviews were conducted with beekeepers in Java and Sumatra, primary islands with significant A. mellifera activities, along with data collection from other regions from January 2022 to October/November 2023. Monthly observations centered on honey production and prices for industrial purposes. The study uncovered two beekeeping systems: migratory in Java and stationery in Sumatra. Carpa honey from Accacia crassicarpa plantation emerged as a suitable industrial honey, displaying sustainable production, seasonality, and a large carpa area as bee forages, contributing to colony growth. Conversely, four Java honey types showed lower productivity, indicating a potential absence of honey seasons. Carpa honey prices ranged from IDR 25,200 to IDR 31,000 per kg, influenced by market size, production, competition, productivity, costs, and producer-consumer interactions. Despite a pandemic-induced surge in the honey market, sustaining high productivity and increasing colony numbers are crucial for long-term honey production sustainability.

Apis cerana Fabricius, 1793 in Sumatra: Haplotype Variations of Mitochondrial DNA and the Molecular Relationship with the Asian Honey Bees (Hymenoptera: Apidae) Simanjuntak, Juniarto Gautama; Priawandiputra, Windra; Raffiudin, Rika; Shullia, Nurul Insani; Jauharlina, Jauharlina; Pradana, Mahardika Gama; Meilin, Araz; Jasmi, Jasmi; Pujiastuti, Yulia; Lestari, Puji; Ilyasov, Rustem; Sitompul, Rahmadi; Atmowidi, Tri
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.768-780

Honey bee Apis cerana is widely distributed in Asia and the Indonesian archipelago, including Sumatra. We studied the molecular variations of A. cerana using cytochrome c oxidase subunits 1 and 2 genes (cox1 and cox2) and the cox1/cox2 intergenic spacers (igs) in several altitudes in the six provinces of Sumatra. We explored the haplotype distributions of those three mtDNA markers for A. cerana in the low-, mid-, and highlands of Sumatra. We also analyzed their relationship with A. cerana in Sundaland and Asia using those markers. Our study revealed 12 new haplotypes of A. cerana cox1 in Sumatra, while nine and eight new haplotypes for cox2 and igs, respectively. Apis cerana in North Sumatra, Lampung, and South Sumatra had the three highest haplotype variations. Most of the specific haplotypes of inter-colony A. cerana from Sumatra were found in the lowlands, while most were in the highlands for intra-colony variations. We found low gene flow among populations of A. cerana in Sumatra. One haplotype, Sumatra4 cox2 from North Sumatra, was the same as Java3 haplotype, presumably due to anthropogenic impact. The molecular phylogenetic tree of A. cerana in the Sundaland revealed that A. cerana from Sumatra has a close relationship to those of Borneo compared to Java.

Comparing Effectiveness of Hand Pollination, Wild Insects and Local Stingless Bees (Tetragonula laeviceps) for Pollination of Exotic Mauritius Raspberry (Rubus rosifolius) Putra, Ramadhani; Dewangga, Rezha Tanu; Hermawan, Endang; Kinasih, Ida; Raffiudin, Rika; Soesilohadi, RC Hidayat; Purnobasuki, Hery
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1050-1060

Mauritius raspberry (Rubus rosifolius) is an exotic plant cultivated in Indonesia. Studies showed that the productivity of exotic plants is reducing due to a lack of pollination agent for their origin region. Several methods could be applied to overcome this problem. The study aimed to find the most effective method for pollinating this plant among four pollination regimes: Self-pollination, wild insects, stingless bees (Tetragonula laeviceps), and hand-pollination. The observation was conducted on the insect pollinators' activities (visitation rate, flower constancy, and flower handling time), pollination efficiency, and quality of the fruits produced (fruit volume). The results showed a higher visitation rate (10 to 70 per hour), higher flower constancy (visited from 07.00 to 16.00), and longer flower handling time (13.6 s) of T. laeviceps on raspberry flowers than other insects. High activities related to better raspberry pollination success (96%) and bigger fruits produced. Based on this study, applying stingless bees as pollination agents for exotic plants was the best and potentially applicable to other exotic crops.

Co-Authors , Triadiati ACHMAD FARAJALLAH Afifah, Ismayati Afriani, Nita Agmal Qodri Agung Prasetyo Alexander Kurniawan Sariyanto Putera Alfath Fanidya ALFIYAN, ACHMAD Ali Agus Amrulloh, Rosyid Andi Gita Maulidyah Indraswari Suhri ANDY DARMAWAN Anzalia Eka Putri Aoliya, Nur Araz Meilin Ariani, Nunik Sri ARIF MUKHTAR HASBI Arismayanti Eka Armowidi, Tri Bambang Purwantara Behling, Hermann Berry Juliandi BINTAR, ANIFA DAMAYANTI BUCHORI Dedy D. Solihin Dewangga, Rezha Tanu DEWI SARTIAMI Dewi, Aisyah Nurlatifah Diana, Akso Dodik Ridho Nurrochmat Dwika Bramasta DYAH PERWITASARI -FARAJALLAH Dyahastuti, Meis endang S. Ratna Erin Treanore Fadliansyah, Fadliansyah Fahri Fahri Farajallah, Ahmad Febiriani, Tia Vina Fenny Oktavia Ferbiyanto, Andri Fifi Gus Dwiyanti Fitradiansyah, La Ode Fitradiansyah Fitriya, Jazirotul Fitriya, Jazirotul Gautama Simanjuntak, Juniarto Gina Dania Pratami, Gina Dania Giyanto Giyanto Grass, Ingo HARIPRABOWO, LEKSONO EKOPURANTO Hasan, M. Ubaidilah Helmi Romdhoni Hepburn, Randall Hepburn, Randall Hermann Behling Hermawan, Endang Hery Purnobasuki Hutabarat, Desmina Kristiani I MADE SAMUDRA I Made Samudra I Nyoman Widiarta IBNUL QAYIM Ida Kinasih Idham Sakti Hararap Ilyasov, Rustem Iman Rusmana Indradewa, Rhian Innayah Zahara IPB, BPKB Irmanida Batubara ISKANDAR ZULKARNAEN SIREGAR Jahnk, Svea Lina Jasmi Jasmi Jasmi Jasmi Jauharlina Jauharlina Jauharlina Jauharlina Jayawarsa, A.A. Ketut Jesajas, David Reinhard KANTHI ARUM WIDAYATI Kuntadi Kuntadi, Kuntadi Lamerkabel, J. S. A. Li, Kevin Listyowati, Sri Mahardika Gama Pradana Meiry F. Noor Mubin, Nadzirum Muhammad Basrowi N. Nurjanah Nadya Aulia Putri Nina Ratna Djuita Nisa, Nisfia Rakhmatun Nisa, Weni Khoiru Nisfia Rakhmatun Nisa NOVA MUJIONO Novidayanti, Aldea Anisyafera NUNIK SRI ARIYANTI Nurjanah Nurul Insani Shullia Nyoto Santoso PERSADA, ALAM PUTRA Pradana, Mahardika Gama Pratiwi, Intan Ratu PUJI LESTARI Puji Lestari Pujiastuti Pulungan, Zumaida Nur Purwanto, Didik B. Putra, Heriansyah Putra, Ramadhani Putri Amelya, Mega Qashiratuttarafi Qashiratuttarafi R.C. Hidayat Soesilohadi Rachman, Muhammad Dhiya'ur Ramadhani Eka Putra Ratna Djuita, Nina RAUF, AUNU RC Hidayat Soesilohadi REZKI, REZKI Rika Indri Astuti Rina Mardiana Rismayanti Robo, Rusnia J Rosadela, Fitri Rosi Fitri Ramadani Rusnia J Robo Ruth Martha Winnie Sabella, Yurika Nur SAHLAN, MUHAMAD SARI, ADHA Sayusti, Tiara Setya Priambudi , Abyan Shafira Ananda Djakaria Siffahk, Lutmitha Nisaul Sih Kahono Sih Kahono Simanjuntak, Juniarto Gautama Siria Biagioni Siti Latifa Wulandari Sitompul, Rahmadi Soemartono Sosmarsono Susanti, Fera Susanti TARUNI SRI PRAWASTI TARUNI SRI PRAWASTI Tiara Sayusti Tri Atmowidi TRI BASTUTI PURWANTINI TRI HERU WIDARTO Uci Sugiman Utami, Anisa Dwi Vella Nurazizah Djalil Vinna Windy Putri Wachju Subchan Widjaja, Candra WIDJAJA, M. CHANDRA Widjaja, Mochammad Chandra Wildan Arsyad F WINDRA PRIAWANDIPUTRA, WINDRA Winnie, Ruth Martha Woro Anggraitoningsih Noerdjito Yelin Adalina Yodhy, Lintang Yofian Anaktototy YULIA PUJIASTUTI Yulia Pujiastuti YULIN LESTARI Zahra Rodliyatam Mardliyah

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