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Temporal resource partitioning of the flight activities of three bee species in East Java: Pembagian sumber daya temporal dari aktivitas terbang tiga spesies lebah di Jawa Timur Shullia, Nurul Insani; Subchan, Wachju; Raffiudin, Rika; Atmowidi, Tri; Priawandiputra, Windra; Ariani, Nunik Sri; Pujiastuti; Dewi, Aisyah Nurlatifah; Sabella, Yurika Nur; Siffahk, Lutmitha Nisaul; Nisa, Weni Khoiru; Novidayanti, Aldea Anisyafera
Jurnal Entomologi Indonesia Vol 21 No 3 (2024): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.3.234

Abstract

The dwarf honey bee Apis florea Fabricius, was successful coexistence with larger bees, Apis cerana Fabricius and Apis dorsata Fabricius in Bangalore, India. However, there is a lack of A. florea foraging behavior that shared the same plant resources with A. cerana and stingless bee Tetragonula laeviceps (Smith) in Indonesia. This study investigated the foraging activities of two honey bee species (A. florea and A. cerana) and the stingless bee T. laeviceps, which live sympatrically and also seek environmental factors that influence the foraging behavior of bees. The flight activity was recorded on the bee farm at Jombang Regency, East Java Province, from 06.00–16.00 hours for three consecutive days. Environmental factors of temperature, humidity, and light intensity in open and close to the nest areas were recorded. This study reported that A. florea started foraging activity at 09.00, while A. cerana and T. laeviceps started to forage earlier, at 06.00 in the morning. Therefore, the foraging activities of bees in East Java, revealed temporal resource partitioning, which confirmed the results of a previous study in Bangalore. The temperature and humidity mainly influenced the foraging activity of the three bee species (P<0,001). Temporal resource partitioning in A. florea suggests a foraging strategy that coexists with sympatric honeybees and stingless bees. The high flight activity of A. florea at midday suggests that this species can adapt to high temperatures. This result implies that A. florea could be a potential future pollinator in tropical regions facing the issue of a warming climate.
Characterization and Identification of Cellulolytic Bacteria from gut of Worker Macrotermes gilvus Ferbiyanto, Andri; Rusmana, Iman; Raffiudin, Rika
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (760.677 KB) | DOI: 10.4308/hjb.22.4.197

Abstract

As a social insect, termite colony consists of three castes, i.e. reproductive, soldier, and worker castes. In their role of cellulose digestion, the worker termites use two sources of cellulolytic enzyme that include cellulases produced by the termite and the gut symbions. Macrotermes gilvus classified in mound builder termite, mostly depend on cellulolytic bacteria for cellulose digestion. This study aims to characterize cellulolytic bacteria of termite gut symbionts of worker M. gilvus and to identify the cellulolytic bacteria based on sequences of 16S ribosomal RNA (rRNA) gene. Cellulolytic bacteria of termite gut were isolated and cultured in CMC (Carboxymethyl cellulose) media. The biochemical characters of bacterial isolates were assayed using Microbact 12A and 12B. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The bacterial isolate that has the highest cellulolytic index was analyzed for its 16S rRNA gene sequences. Four isolates of cellulolytic bacteria were successfully isolated from gut of M. gilvus with aerobic and anaerobic conditions. The highest formation of cellulolytic index (2.5) was revealed by RA2. BLAST-N (Basic Local Alignment Search Tool for Nucleotides) result of 16S rRNA gene sequences of RU4 and RA2 isolates showed that the isolate has similarity with Bacillus megaterium and Paracoccus yeei, respectively. This result indicated that RA2 isolate was P. yeei, a cellulolytic bacterium of a termite gut of M. gilvus.
CURRENT STATUS OF APIS MELLIFERA BEEKEEPING IN SUMATRA: TOWARD THE DEVELOPMENT OF INDONESIA'S HONEY INDUSTRY Purwanto, Didik B.; Purwantini, Tri Bastuti; Diana, Akso; Raffiudin, Rika; Sahlan, Muhamad
International Journal of Social Service and Research Vol. 5 No. 2 (2025): International Journal of Social Service and Research
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/ijssr.v5i2.1196

Abstract

Apis mellifera beekeeping in Sumatra has seen significant growth over the past decade, particularly since the COVID-19 pandemic began in early 2020, driven by high market demand for honey. Beekeeping activities are concentrated in Riau and Jambi provinces, where beekeepers primarily utilize extrafloral nectar from Acacia crassicarpa plantation forests. This study evaluates the current state of A. mellifera beekeeping in these regions, focusing on beekeeper proficiency and colony productivity. Data were collected through a survey of 42 beekeepers, addressing their characteristics, efforts to improve skills, perceptions of challenges, and colony productivity in 2024. The findings indicate that most beekeepers are young males aged 30–50 with secondary school education, managing between 100 and 500 hives despite limited knowledge. They primarily rely on self-directed learning and peer knowledge-sharing. Most beekeepers rated their apiaries as satisfactory but recognized their need for guidance to enhance productivity, ensure sustainability, and improve market access. In 2024, honey production averaged 25 kg per colony per year, or 2.1 kg per month, with Jambi slightly outperforming Riau (2.25 vs. 2.0 kg/colony/month). Honey prices averaged IDR 22,000/kg, with Riau prices higher (IDR 25,500-26,000/kg) than Jambi (IDR 19,800-20,300/kg). Sumatra is estimated to host around 250,000 A. mellifera colonies, contributing 92% to national honey output, compared to Java's 46,000 colonies and 8% of production.
Analisis Keragaman Komunitas Bakteri pada Lambung Madu Lebah Apis mellifera, Apis cerana, dan Tetragonula laeviceps: Analysis of Communities Bacterial Diversity on Bees Honey Stomach from Apis mellifera, Apis cerana, and Tetragonula laeviceps Rosadela, Fitri; Rusmana, Iman; Nurjanah; Raffiudin, Rika
Jurnal Sumberdaya Hayati Vol. 11 No. 2 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.2.38-48

Abstract

Bees are one of the pollinators that a honey producing agent as well. Honey derives from nectar that stored and procees in honey stomach. The quality of honey is determined by physical, chemical and microbes in the honey stomach as well. This study was aimed to analyze the diversity of bacterial communities (bacterial metagenome) derived from bee’s honey stomach of A. mellifera, A. cerana, and T. laeviceps using Denaturing Gradient Gel Electrophoresis (DGGE) method. Previous researches of bacterial communities are more focused on the entire digestive tract, which is not specifically in honey stomach of the bees. The diversity of bacterial communities showed differences among three bee’s species. The number one of A. cerana has the highest number of Operational Taxonomic Units (OTUs), i.e., 15 OTUs compared to the other sample. While the T. laeviceps number two show the highest percentage of OTU dominant at 59.4%. The bacterial community in bees honey stomach of A. mellifera, A. cerana, T. laeviceps the similarity of bacteria from phylum Firmicutes, Proteobacteria, Acidobacteria and Fusobacteria. Actinobacteria and Bacteroidetes phyla are found only in A. cerana honey stomach.
Melissopalynological Analysis Revealed a Higher Pollen Diversity in Stingless Bees than in Honey Bees Sayusti, Tiara; Raffiudin, Rika; Djuita, Nina Ratna; Perwitasari-Farajallah, Dyah; Batubara, Irmanida; Priawandiputra, Windra; Sitompul, Rahmadi; Jahnk, Svea Lina; Behling, Hermann
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.196-203

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Identifying floral resources used by honey bees and stingless bees is essential for sustainable beekeeping and understanding pollination ecology. This study compared the diversity of floral resources utilized by the honey bee Apis cerana and stingless bees Geniotrigona thoracica and Heterotrigona itama based on pollen in honey. Bee colonies were in the same Flora Nauli Beekeeping area, Pematang Siantar, North Sumatra, Indonesia, ensuring equal access to similar surrounding floral resources. Honey samples were collected, and pollen grains were extracted, acetolysed, and analyzed by counting 1,200 grains to determine frequency classes. Pollen diversity was calculated using the Shannon–Wiener index (H′). Stingless bee honey contained more diverse pollen than A. cerana, which only contained pollen from the Arecaceae family. Heterotrigona itama and G. thoracica honey contained pollen from 20 and 11 plant families, respectively. Each species had a distinct predominant pollen type (>45%): Cocos nucifera (A. cerana), Sapotaceae type (G. thoracica), and Casuarinaceae type (H. itama). A higher pollen diversity index was also observed in H. itama (1.05-1.83) than in G. thoracica (0.34–1.64) and A. cerana (0.66). These results indicate that stingless bees are more generalist than honey bees, highlighting their ecological role in supporting pollination networks in tropical agroecosystems.
Strategi Konservasi dan Pelestarian Lebah di Indonesia Buchori, Damayanti; Priawandiputra, Windra; Kahono, Sih; Raffiudin, Rika; Putra, Ramadhani Eka; Armowidi, Tri; Meilin, Araz; Sari, Adha; Amrulloh, Rosyid; Sartiami, Dewi
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 5 No 3 (2023): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0503.690-694

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Rekomendasi kebijakan yang perlu dilakukan dalam Strategi Konservasi dan Pelestarian Lebah di Indonesia: 1) Penghentian alih fungsi lahan dari hutan menjadi perkebunan dan pertanian monokultur 2) Penggunaan pestisida yang bijaksana 3) Memperbanyak jumlah dan keragaman tanaman penyedia pakan lebah 4) Mempromosikan taman kehati dan ruang terbuka hijau yang ramah lebah 5) Pengembangan kawasan konservasi untuk habitat lebah di perkebunan 6) Mempromosikan konsep pertanian regenerative 7) Meningkatkan peran petani dalam pertanian berkelanjutan yang ramah lebah 8) Pembuatan kebijakan untuk menetapkan spesies lebah yang terancam punah
Variasi perilaku mencari makan pada semut rangrang Oecophylla smaragdina (Fabricius) pada habitat yang berbeda: Variations of foraging behavior of weaver ants Oecophylla smaragdina (Fabricius) in different habitats Rezki, Rezki; Aoliya, Nur; Fadliansyah, Fadliansyah; Wulandari, Siti Latifa; Jesajas, David Reinhard; Raffiudin, Rika
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.141

Abstract

Weaver ants (Oecophylla smaragdina (Fabricus)) are carnivorous insects and play a role as biocontrol agents in urban habitats. This study aims to analyze the foraging behavior of O. smaragdina in two different habitats, namely urban and non-urban. The two locations are characterized by differences in the intensity of human interaction and the distance between the trees and the tree canopy. The behavioral observation method uses all sampling events with five behavioral categories: lurking (M1), approaching (M2), carrying (M3), communicating between ants in a colony (K1), and competition with other ants (K2). We tested three different types of feed: chick scraps, Tenebrio molitor Linnaeus larvae, and sugar. The results showed that there were three sequences of foraging behavior in urban areas, namely: (1) M1, M2, K1, M3, (2) M1, M2, K1, M3, K2, and (3) M1, M2, K2, M3. Meanwhile, there is only one pattern in non-urban areas, namely M1, M2, M3. Approaching food (M2) and lurking food (M1) were the dominant responses of O. smaragdina in urban and non-urban areas. In urban areas, broiler chickens were the type of feed most approached by ants, followed by sugar and beetle larvae. O. smaragdina in non-urban areas also preferred chicken pieces as feed compared to the others, but only one individual O. smaragdina approached (M2) chicken pieces and none approached the others. Foraging ants are more active at high temperature and low humidity, compared to low temperature and high humidity. Therefore, the foraging behavior of O. smaragdina is influenced by habitat factors.
The characterization of Apis cerana and Apis mellifera mrjp2 gene in Indonesia and the phylogeny relationship with mrjp family genes: Karakterisasi gen mrjp2 Apis cerana dan Apis mellifera di Indonesia dan hubungan filogeni dengan gen mrjp famili Shullia, Nurul Insani; Febiriani, Tia Vina; Raffiudin, Rika; Widjaja, Candra; Atmowidi, Tri
Jurnal Entomologi Indonesia Vol 21 No 1 (2024): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.1.1

Abstract

The major royal jelly protein 2 (mrjp2) gene is one of the molecular markers that can discriminate between Apis cerana Fabricius and A. mellifera Linnaeus. Due to the lack of mrjp2 gene sequences registered in GenBank for Indonesian A. cerana and A. mellifera, DNA characterization and bioinformatics analysis were needed. This research aimed to characterize the exon-intron organization of mrjp2 genes for both Indonesian bee species and analyze the phylogenetic relationship with other mrjp family genes. DNA samples of Apis cerana and A. mellifera, collected from Bogor, were amplified using MF-MR primer at annealing temperatures of 47 oC and 50 oC, respectively. The length of A. cerana and A. mellifera DNA sequences were 579 and 597 bp, respectively. The DNA sequences of both species were comprised of partial exons 1, introns 1, exons 2, introns 2, and partial exon 3. The number of putative amino acids of A. cerana and A. mellifera mrjp2 genes were 111 and 123, respectively. We confirmed that the partial MRJP2 putative amino acids of both honey bees belong to the MRJP family and contained the peptide signal in the 14 first amino acid sites. Nucleotide variation of the mrjp2 gene in A. cerana was higher than A. mellifera. Phylogenetic tree construction showed that A. cerana and A. mellifera form a monophyletic clade with the A. mellifera mrjp7 gene and another mrjp family gene clustered as reported in a previous study.
RAPID DETECTION OF THE AFRICANIZED HONEY BEE: A TOOL FOR INDONESIAN ANIMAL QUARANTINE RAFFIUDIN, RIKA; BINTAR, ANIFA; WIDJAJA, M. CHANDRA; FARAJALLAH, AHMAD; PURWANTARA, BAMBANG
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.086 KB) | DOI: 10.11598/btb.2009.16.1.65

Abstract

Molecular detection methods were used to determine if Africanized Honey Bees (AHBs) are present in populations of imported Apis mellifera in Indonesia. The cytochrome b (cyt b gene) was amplified from mitochondrial DNA and digested with the BglII restriction enzyme (cytb/BglII). Two types of animal DNA extraction kits were used and found suitable for rapid preparation of DNA from A. mellifera by the Animal Quarantine facility. Results showed that all 94 colony samples from beekeepers in Java produced a 485 bp PCR product from the amplification of this gene. Two DNA fragments of 194 and 291 bp from all samples were produced after digestion with BglII. This cytb/BglII result together with the DNA sequence of cyt b showed that all collected samples of A. mellifera were the non-AHB type. Hence, this study did not detect AHB in Indonesia.Key words :  Apis mellifera, molecular detection methods, DNA, cytochrome b, mitochondrial genetics
INTRASPECIFIC VARIATIONS OF 16S MITOCHONDRIAL GENE SEQUENCES OF YELLOW RICE STEM BORER,scirpopbaga incertulas (LEPIDOPTERA: CRAMBIDAE) FROM WEST JAVA RAFFIUDIN, RIKA; WINNIE, RUTH MARTHA; SAMUDRA, I MADE
BIOTROPIA Vol. 18 No. 1 (2011): BIOTROPIA Vol. 18 No. 1 June 2011
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2011.18.1.135

Abstract

Yellow rice stem borer (Scirpophaga incertulas) is one of the most important rice pest insects in Asia, including Indonesia. However, there is a lack of genetic data for this important agricultural insect.  Therefore, this study was conducted to explore intraspecific differentiation of S. incertulas partial 16S mitochondrial gene from Bogor, Karawang, Indramayu and Cirebon (West Java, Indonesia). Here, we reported a total of 325 bp of 16S mitochondrial gene of S. incertulas from the obtained samples. Among all DNA sequences, three haplotypes of 16S mitochondrial gene were observed and submitted to GenBank under Accession Number of GU191881, GU191882, GU191883, respectively for haplotype 1, 2, and 3.  The haplotype 1 was found in all S. incertulas surveyed locations, except Bogor. The haplotype 2  and 3 were found only in from Cirebon and Bogor samples. These haplotype variations can be applied as DNA markers for S. incertulas early larva detection method among other rice stem borers. Hence, further explorations of the mitochondrial variations of S. incertulas in Java and other parts of Indonesia are needed.   Keywords: moth, haplotypes, genetic differentiations,  molecular identification
Co-Authors , Triadiati ACHMAD FARAJALLAH Afifah, Ismayati Afriani, Nita Agmal Qodri Agung Prasetyo Alexander Kurniawan Sariyanto Putera Alfath Fanidya ALFIYAN, ACHMAD Ali Agus Amrulloh, Rosyid Andi Gita Maulidyah Indraswari Suhri ANDY DARMAWAN Anzalia Eka Putri Aoliya, Nur Araz Meilin Ariani, Nunik Sri ARIF MUKHTAR HASBI Arismayanti Eka Armowidi, Tri Bambang Purwantara Behling, Hermann Berry Juliandi BINTAR, ANIFA DAMAYANTI BUCHORI Dedy D. Solihin Dewangga, Rezha Tanu DEWI SARTIAMI Dewi, Aisyah Nurlatifah Diana, Akso Dodik Ridho Nurrochmat Dwika Bramasta DYAH PERWITASARI -FARAJALLAH Dyahastuti, Meis endang S. Ratna Erin Treanore Fadliansyah, Fadliansyah Fahri Fahri Farajallah, Ahmad Febiriani, Tia Vina Fenny Oktavia Ferbiyanto, Andri Fifi Gus Dwiyanti Fitradiansyah, La Ode Fitradiansyah Fitriya, Jazirotul Fitriya, Jazirotul Gautama Simanjuntak, Juniarto Gina Dania Pratami, Gina Dania Giyanto Giyanto Grass, Ingo HARIPRABOWO, LEKSONO EKOPURANTO Hasan, M. Ubaidilah Helmi Romdhoni Hepburn, Randall Hepburn, Randall Hermann Behling Hermawan, Endang Hery Purnobasuki Hutabarat, Desmina Kristiani I MADE SAMUDRA I Made Samudra I Nyoman Widiarta IBNUL QAYIM Ida Kinasih Idham Sakti Hararap Ilyasov, Rustem Iman Rusmana Indradewa, Rhian Innayah Zahara IPB, BPKB Irmanida Batubara ISKANDAR ZULKARNAEN SIREGAR Jahnk, Svea Lina Jasmi Jasmi Jasmi Jasmi Jauharlina Jauharlina Jauharlina Jauharlina Jayawarsa, A.A. Ketut Jesajas, David Reinhard KANTHI ARUM WIDAYATI Kuntadi Kuntadi, Kuntadi Lamerkabel, J. S. A. Li, Kevin Listyowati, Sri Mahardika Gama Pradana Meiry F. Noor Mubin, Nadzirum Muhammad Basrowi N. Nurjanah Nadya Aulia Putri Nina Ratna Djuita Nisa, Nisfia Rakhmatun Nisa, Weni Khoiru Nisfia Rakhmatun Nisa NOVA MUJIONO Novidayanti, Aldea Anisyafera NUNIK SRI ARIYANTI Nurjanah Nurul Insani Shullia Nyoto Santoso PERSADA, ALAM PUTRA Pradana, Mahardika Gama Pratiwi, Intan Ratu PUJI LESTARI Puji Lestari Pujiastuti Pulungan, Zumaida Nur Purwanto, Didik B. Putra, Heriansyah Putra, Ramadhani Putri Amelya, Mega Qashiratuttarafi Qashiratuttarafi R.C. Hidayat Soesilohadi Rachman, Muhammad Dhiya'ur Ramadhani Eka Putra Ratna Djuita, Nina RAUF, AUNU RC Hidayat Soesilohadi REZKI, REZKI Rika Indri Astuti Rina Mardiana Rismayanti Robo, Rusnia J Rosadela, Fitri Rosi Fitri Ramadani Rusnia J Robo Ruth Martha Winnie Sabella, Yurika Nur SAHLAN, MUHAMAD SARI, ADHA Sayusti, Tiara Setya Priambudi , Abyan Shafira Ananda Djakaria Siffahk, Lutmitha Nisaul Sih Kahono Sih Kahono Simanjuntak, Juniarto Gautama Siria Biagioni Siti Latifa Wulandari Sitompul, Rahmadi Soemartono Sosmarsono Susanti, Fera Susanti TARUNI SRI PRAWASTI TARUNI SRI PRAWASTI Tiara Sayusti Tri Atmowidi TRI BASTUTI PURWANTINI TRI HERU WIDARTO Uci Sugiman Utami, Anisa Dwi Vella Nurazizah Djalil Vinna Windy Putri Wachju Subchan Widjaja, Candra WIDJAJA, M. CHANDRA Widjaja, Mochammad Chandra Wildan Arsyad F WINDRA PRIAWANDIPUTRA, WINDRA Winnie, Ruth Martha Woro Anggraitoningsih Noerdjito Yelin Adalina Yodhy, Lintang Yofian Anaktototy YULIA PUJIASTUTI Yulia Pujiastuti YULIN LESTARI Zahra Rodliyatam Mardliyah