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All Journal Media Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Pharmaceutical and Sciences. Buletin Pengabdian Jurnal Agro Veteriner
Suzanita Utama
Department Of Veterinary Reproduction - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Kedokteran Hewan

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THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Dhesy Kartikasari; Sri Mulyati; Suzanita Utama; Pudji srianto; Widjiati Widjiati; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.285 KB) | DOI: 10.21157/j.ked.hewan.v14i1.15283

Abstract

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Kartikasari, Dhesy; Mulyati, Sri; Utama, Suzanita; srianto, Pudji; Widjiati, Widjiati; Plumeriastuti, Hani
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15283

Abstract

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis.A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL.The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies.Positive results showed a brownish color on the oocyte and its cumulus.The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased.It can be concluded that the addition of urea does not affect the level of apoptosis.
Co-Authors . Helen . Mulyani . Purwati Abdul Samik, Abdul Abdullah Hasib Adikara, Yuanara Augusta Rahmat Akbar Haryo Widhi Trismiharto Al arif, Mohammad Anam Alfanindya, Evita Aisyah Amrullah, Muhammad Fajar Belgania, Ristaqul Husna Berliana, Erprinanda Galuh BUDI UTOMO Budi Utomo Budiarto Budiarto Candra Bumi Chairul Anwar Chandra, Evania Haris Chandramohan, Koshini Desak Ketut Sekar Cempaka Putri Dhesy Kartikasari Diba, Lucky Djoko Legowo Djoko Poetranto DWI WIJAYANTI Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati ERMA SAFITRI Erma Safitri Fedik Abdul Rantam Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove Ida Yuniarti IMAM MUSTOFA Imam Mustofa Ira Sari Yudaniayanti Ismudiono Ismudiono, Ismudiono Kadek Rachmawati Kadek Racmawati Kartikasari, Dhesy Kevin Mardotillah Khairullah, Aswin Rafif Koesnoto Supranianondo Kuncorojakti, Suryo Laba Mahaputra Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mas’ud Hariadi Mayola, Dilla Aranda Meles, Dewa Ketut Mochamad Lazuardi Muhammad Fahmi Abdillah Muhammad Faizal Adiutomo Ngakan Made Rai Widjaja Niluh Suwasanti Nunuk Dyah Retno Lastuti Nurhusien Yimer Nusdianto Triakoso Pratama, Hanif Sabekti Primarizky, Hardany Pudji Srianto Putri, Desak Ketut Sekar Cempaka R. Heru Prasetyo R.T.S Adikara, R.T.S Adikara Racmawati, Kadek Ratna Damayanti Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Santoso, Kuncoro P. Sarudji, Suryanie Setiawan Koesdarto Sharveihn Sathindren Soeharsono Soeharsono Sofie Adi, Keren Jayanti Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Wigati Mardi Suherni Susilowati Sukmanadi, Moh. Sukmanadi, Mohammad Sulaiman, Azhari Athaillah Suwasanti, Ni Luh Suwasanti, Niluh Syaiful Rizal Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Warsito, Sunaryo Hadi Widjiati w Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wurlina, W Xin, Irene Teh Kai Yunus, Muchammad