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Eka Safitri
STMIK AMIKOM Yogyakarta
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Journal : Jurnal Natural

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The validation of urea determination in saliva using optical urea biosensor with p-dimethylaminobenzaldehyde (DMAB) reagent ALDIANSYAH, TEUKU; SAFITRI, EKA; GINTING, BINAWATI; NAZARUDDIN, NAZARUDDIN; SUHUD, KHAIRI; SALEHA, SITTI; NURHAYATI, NURHAYATI; NAZARUDDIN, SALSABILLA LATANSA; AFIFI, MUHAMMAD RIDHO; NURHAIDA, NURHAIDA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.41251

Abstract

The validation of urea determination in saliva using the biosensor method and the p-dimethylaminobenzaldehyde (DMAB) method has been successfully conducted. Anthocyanin compounds from Chatarantus roseus flowers were extracted using the maceration method with methanol as the solvent, yielding a total of 22.60% with a total concentration of 6.01 mg/L. The obtained extract was subjected to qualitative anthocyanin testing, showing a positive result was indicated by the formation of a reddish color and quantitative testing with the formation of a faded yellow color. The maximum wavelength ( max) of anthocyanin was 664 nm with an absorbance of 0.674. The saliva samples were obtained from three different ages i.e: children, teenagers, and adults. During the saliva collection procedure, participants were instructed to abstain from consuming any food or beverages, with the exception of water, for a minimum of 1 hour prior to sample collection. All samples were stored in a freezer at 4-8C until needed for analysis. Linearity test results were assessed using a calibration curve, yielding a coefficient of determination (R) of 0.978 with a sensitivity of 0.033 for the biosensor and an R of 0.975 with a sensitivity of 0.685 for DMAB. The limit of detection (LOD) values were determined as 7.203 10 M for the biosensor and 6.984 10 M for DMAB, while the limit of quantification (LOQ) values were 2.182 10 M and 2.116 10 M, respectively. Statistical analysis using a t-test showed tcalculated = 1.314, compared to ttable = 4.302, indicating that tcalculated ttable. This result suggests no statistically significant difference between the biosensor and DMAB methods, confirming that the biosensor method is comparable in performance to the DMAB method. Additionally, urea concentration measurements showed that adult samples exhibited the highest urea levels among the tested samples, which may indicate an association with potential health risks, including dental caries, kidney failure, and liver damage.
Co-Authors Abdul Karim Achmad Yanu Aliffianto Afifi, Muhammad Ridho Agung Hartoyo Ahmad Mujahid, Ahmad Ahmad, Nursaidah Akastangga, Muhammad Dedad Bisa Raguna Al Af Ghoni, Achmad Solakhuddin Al Khasy, Moh. Ali Qorror ALDIANSYAH, TEUKU Alexander Hamonangan Simamora Alghiffary, Muhammad Amalia, Rika Sri Andika, Iswandi Anita Anita Annas Alkhowarizmi Aprinal Adila Asril Arfa Azkiyah, Desintya Arifin , Atwal Arifin, Ahmad Kamal Arsyad, Muhammad Rizal Aviv Pasa, Muhammad Awalia, Hu’shilla Azis, Nurul Ni'ma Azmi, Abdullah Binawati Ginting Bistari Budi Sustriawan Budiman, Muh. Arief Chandra, Novrina Cloudya, Afifah Dani Rizana Dayanti, Meri Dedi Junaedi, Dedi Dedri Syafei Dodi Irwandi, Dodi Edi Suharto Evi Novita Evi Widowati EVY FITRIA, EVY Fahruddin Ali Sabri Fazriani, Eva Tatia Fega, Nabila Fera Sriyunianti Fitri Dara Fitria Budi Utami Fitriyana Fitriyana, Fitriyana Fonna, Syafrizal Ginting, Rahmadani Ftri Hadi, Mukhammad Nur Hanum, Dania Febyola Harisa, Muh. Hermaliana, Mia I Kadek Suartama Ilham Maulana Isti Handayani Jannah, Firaihanil Julinawati, Julinawati Khulduniah, Khulduniah Laksono, Muharram Budi Lelifajri Lelifajri Luthfi, Rantika M. Zulkarnain Maesaroh, Nur Maharani, Alviah Camilla Masum, Ahmad Md Sani, Nor Diyana Mohamed Salem Alawadi, Elsayed Mu'arif, Mohammad Wildan Muhammad Idrus Muhammad Ihsan Muhammad Isa Muhsin Riyadi MUNASIB MUNASIB, MUNASIB Mustanir Mustanir Mustaufik N. Nazaruddin Nasrullah Idris NAZARUDDIN, SALSABILLA LATANSA Nina Herlina Nur Aini Nur Huda Nurhaida Nurhaida Nurhayati Nurhayati Priyono, Habib Putri, Najwa Auliya Qodri Rahil Helmi Rahmadi Rahmadi Ramadani, Nurista Riski Rikki Vitria Rinaldi Idroes Riski, Novia Riza Chamadi, Muhammad Riza Multazam Luthfy Rosalina, Ranti Resmi Rumpoko Wicaksono Sa'dudin, Ihsan Sa’dudin, Ihsan Sagir Alva Sa’dudin, Ihsan Sinaga, Sunita Siregar, Nasrun Salim Siti Aminah SITI KHOLIFAH Siti Mujanah Siti Nurhayati Sitti Saleha, Sitti Sontani, Uep Tatang Suci Maulina Suedah, Suedah Suhud, Khairi Sukoma, Sukoma Supriatno Suriani, Seri Suwardinata, Amiliya Syatta, Indy Mafiiqo Taher, Rusdee Ulfah Ulfah Umamah, Umamah Wahyuni, Eni Wirawan Fadly Yuliah, Elah Yulius Saprianus Dala Ngapa Zuliaha, Isnaini