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All Journal Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology JPM: JURNAL PENGABDIAN MASYARAKAT

Trilas Sardjito

Departmen Reproduksi Veteriner Fakultas Kedokteran Hewan, Universitas Airlangga Kampus-C Unair, Jl Mulyorejo, Surabaya, Jawa Timur, Indonesia, 60115

Author-ID : 3693330

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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The Antiproliferative and Spermicide Effects of D-Limonene on Gonadal Function in Male Albino Rats: A Comprehensive Study on Spermatogenesis and Gonadal Morphometry Herdiansyah, Akbar Dimas; Yudistira, Neo Rakka; Afandik, Nadya Ashila; Meiliza, Nazwa; Ardiansyah, Kresna Ryan; Sardjito, Trilas; Sasi, Samira Musa
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.149-162

This study investigates the effects of D-Limonene on reproductive health parameters in albino rats, focusing on sperm quality and gonadal morphology. The study involved administering D-Limonene at various doses to assess their impacts on sperm parameters, including motility, viability, and abnormalities, as well as gonadal morphometry. The D-Limonene was applied at doses of 50, 100, and 150 mg/kg BW for 60 days, followed by histopathological analysis of testicular tissues. Results indicated that D-Limonene treatment revealed a dose-dependent decline in sperm quality parameters. Specifically, total motility, progressive motility, and viability decreased, while abnormality rates increased with higher D-Limonene doses. Notably, the highest dose (150 mg/kg BW) led to the most significant reductions in sperm motility and viability. Additionally, the presence of necrotic cells and structural changes in testicular tissues were observed, supporting the adverse impact of D-Limonene on reproductive function. The findings suggest that D-Limonene exhibits potential as an effective spermicide, highlighting its utility in population control applications. The study underscores the need for further research to fully understand the mechanisms behind these effects and their implications for reproductive health.

PENAMBAHAN L – ARGININ DALAM PENGENCER TRIS KUNING TELUR SETELAH EKUILIBRASI 2 JAM TERHADAP MOTILITAS DAN MEMBRAN PLASMA UTUH SPERMATOZOA DOMBA SAPUDI Suryaningsih, Hastini; Sabdoningrum, Emy Koestanty; Susilowati, Suherni; Sardjito, Trilas; wurlina, wurlina; Damayanti, Tita
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This research was aimed to determine the addition of L-arginine on motility and sperm plasma membrane of Sapudi sheep after two hour equilibration time in egg yolk tris diluent. This research used fresh samples of sheep Sapudi semen collected by artificial vagina, then were devided into 4 treatments and 6 replications. Analysis of the data using Analysis of Variance (ANOVA) then proceed to the Duncan Test to determine significant differences between treatments. The first treatment P0 was no L – Arginine added as control. P1 was treated with 0,004 M L – Arginine. P2 was treated with 0,005 M L – Arginine, P3 was treated with 0,006 M L – Arginine. Result of two hour equilibration time for the sperm motility showed: (P0) 46,67a 5,16, (P1) 49,16ab 5,84, (P2) 55,00bc 7,74, (P3) 58,33c 6,83. Result of two hour equilibration time for the sperm plasm membrane were: (P0) 39,33a 4,92, (P1) 43,33ab 4,22, (P2) 46,50 bc 3,44, (P3) 50,83 c 3,48. The addition of L-arginine with a concentration as much as 0.006 M, shows the highest result in sperm motility and sperm plasm membrane intact of Sapudi semen.

CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERAH AKSEPTOR INSEMINASI BUATAN DI KUD ARGOPURO KECAMATAN KRUCIL KABUPATEN PROBOLINGGO Yohana, Nadia; Samik, Abdul; Aksono, Bimo; Sardjito, Trilas; Hermadi, Herry Agoes; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Reproduction is one of essential factor directly affecting profitability in a dairy production system. Some of the reproductive factors that influenced to the developing of dairy cattle of small holder's farmer are Conception Rate (CR) Service per Conception (S/C). The study was conducted to know the CR and S/C of Friesian Holstein in KUD Argopuro, Probolinggo. The data is taken primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variables include : the identity of the breeder, cages, and feeding and drinking. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by Inseminator in KUD Argopuro. The data which by collected from this sample are CR and S/C. This research was a case study. The materials used cattle as the acceptors were 10% from 728 lactation cattle. Data from the results of this research on CR (30%) and S/C (2,49). The conclusion of this research for CR the result were lower than normal and S/C the result were higher than normal.

PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS Nawangwulan, Tantri; Madyawati, Sri Pantja; Plumeriastuti, Hani; Sardjito, Trilas; Susilowati, Suherni; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.

CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERANAKAN LIMOUSIN DAN SAPI PERANAKAN ONGOLE HASIL IB DI KECAMATAN PILANGKENCENG KABUPATEN MADIUN Bhaskara, Alfiutha Propha; Suprayogi, Tri Wahyu; Darsono, Roesno; Srianto, Pudji; Hariadi, Mas'ud; Sardjito, Trilas
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The study was conducted to know conception rate and service per conception of Limousin Cross breed and Ongole Cross Breed in Pilangkenceng, Madiun. Data were collected for the periode of Oktober 2016 to February 2017 from inseminators in Pilangkenceng district. The data were taken by primary and secondary. Primary data retrieval were done by direct observation, which includes several variables, where the variables include: the identity of the breeder, feeding and drinking. The secondary data were obtained by recording the farmer's artificial insemination card. All data was analyzed to find a percentage of conception rate (CR) and find number of service per conception (S/C). Then S/C were analyzed with T-test adn CR were analyzed with chi-square test. The results of this research showed that the service per conception's average of limousin cross breed and ongole cross breed is 1,61 and 1,72. The average of conception rate limousin cross breed and ongole cross breed is 53% % and 59 %. The result showed no significant difference (p>0,05) on CR and S/C between limousin cross breed and ongole cross breed. The conclusion of this research showed that service per conception and conception rate of ongole cross breed in Pilengkenceng Madiun is better than limousin cross breed but there's no significant difference (p>0,05).

PENGARUH LAMA WAKTU PENGHITUNGAN STRAW DAN POSISI JARAK STRAW DIATAS PERMUKAAN NITROGEN CAIR TERHADAP MOTILITAS DAN VIABILITAS SEMEN BEKU SAPI MADURA POST THAWING Ardiana, Lutfi; Hardijanto, Hardijanto; Damayanti, Ratna; Sardjito, Trilas; Mustofa, Imam; Utomo, Budi
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Aims of this research was to determine the effect of the duration of straw count and the position of straw from liquid nitrogen surface to the motility and viability of post thawing frozen Madura cattle. This study used a sample derived from Madura cattle (Pasean OO 1220) from BBIB singosari as much as 54 straw divided into 9 treatments. P1 (1cm 10 sec), P2 (1cm 20 sec), P3 (1cm 40 sec), P4 (2cm 10 sec), P5 (2cm 20 sec), P6 (2cm 40 sec), P7 (3cm 10 sec), P8 (3cm 20 sec), P9 (3cm 40 sec) and repeated as much as 6 times then observed for motility and viability of post thawing spermatozoa. The data obtained were analyzed using SPSS 16.0. The results showed that the duration of straw count and the position of straw from liquid nitrogen significant (P<0,05) on post thawing motility. The position of straw from liquid nitrogen signifant (P<0,05) on post thawing viability. The conclusion of this research showed that 1 cm 40 sec give the higher post thawing motility.

PERANAN VITAMIN B12 DALAM PENGENCER SUSU SKIM KUNING TELUR TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA SAPUDIPOST THAWING Arini, Mirza Asafaizh; Suprayogi, Tri Wahyu; Warsito, Sunaryo Hadi; Sardjito, Trilas; Susilowati, Suherni; Triana, Indah Norma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, Sapudi sheep semen was diluted in the egg yolk skim milk extender + vitamin C 0,02 mg / ml, treatment group (P1 and P2), Sapudi sheep semen was diluted in the egg yolk skim milk extender+ vitamin B12 5 Î¼g / ml and grouped in P1, Sapudi sheep semen was diluted in the egg yolk skim milk extender + Vitamin C 0.02 mg / ml + Vitamin B12 5Î¼g / ml and grouped in P2. The data analysis was use One Way Analysis of Variant (ANOVA), then to identify the real difference among the treatments continued with Duncan distance test. The result of data analysis showed that P2 treatment was significantly different (p <0.05) with P1 treatment and P0 treatment or control. While P1 treatment was not significantly different (p> 0,05) with P0 treatment or control. The result showed that the addition of vitamin C 0.02 mg / ml with vitamin B12 5 Î¼g / ml combination in egg yolk skim milk extender was the best addition to maintain the motility and viability of the post thawing Sapudi sheep spermatozoa.

PENGARUH pH LENDIR MUKOSA VAGINA SAAT BIRAHI TERHADAP PERSENTASE KEBUNTINGAN (Conception Rate) PADA SAPI PERAH DI KUD TANI WILIS KABUPATEN TULUNGAGUNG DAN KSU TUNAS SETIA BARU KABUPATEN PASURUAN Rizki, Andaru; Srianto, Pudji; Suprihati, Endang; Sardjito, Trilas; Ismudiono, Ismudiono; Al Arif, Mohammad Anam
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research is to determine the effect of mucos acidity of vaginal mucous when estrus towards conception rate at KUD Tani Wilis Tulungagung District and KSU Tunas Setia Baru Pasuruan District. Measured samples were taken from 30 dairy cattles in each KUD. Mucos acidity of vaginal mucous were obtained by using a digital acidity meter before insemination was done on dairy cattle. After measuring the acidity from mucosal vagina mucous, artificial insemination has done on estrus dairy cattle. On the 60th day after insemination, rectal palpation was done to check the conception rate. Research result from 30 dairy cattle on each KUD showed that mucos acidity of vaginal mucous has signifficant effect towards conception rate. Mucos acidity of vaginal mucous range 6.5-8.5 with the highest range of conception rate in KSU Tunas Setia Baru Pasuruan District 7.5-7.7 and in KUD Tani Wilis Tulungagung District with the highest range of conception range 7.2-7.4 and 7.5-7.7.

CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI BALI HASIL INSEMINASI BUATAN DI KABUPATEN SUMBAWA BARAT TAHUN 2017 Deskayanti, Asih; Sardjito, Trilas; Sunarso, Agus; Srianto, Pudji; Suprayogi, Tri Wahyu; Hermadi, Herry Agoes
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The study was conducted to know Conception Rate and Service per Conception of Bali cattle acceptors in west Sumbaw district. Data were collected fot the periode of January to September 2017 from inseminator in west Sumbawa district. The data were taken by primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variable include the identitiy of the breeder, the cages, feeding, drinking and knowladge of breeders. The secondary data were obtained by recording of the officer insemination. All data was analyzed to find a percentage of Conception Rate and find number of Service per Conception (S/C) by analyzed descriptive of SPSS. Risults of this research showed that Conception Rate and Service per Conception's average of Bali Cattle is 60 % and 1,7. The conculusion of this research showed that Conception Rate is lower than normal value and Service per Conception of Bali cattle in West Sumbawa district is better. This research was expected to be a reference for the next artificial insemination programmed by the government.

Hubungan antara skor hasil bacaan heat detector dengan gambaran sitologi vagina sebagai parameter penentuan estrus pada kambing Meydilasari, Nanda Irsa; Hernawati, Tatik; Lestari, Tita Damayanti; Safitri, Erma; Sardjito, Trilas; Ismudiono, Ismudiono; Hidanah, Sri
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aimed to determine the relationship between heat detector reading results and the feature of vaginal swab preparations as a parameter for determining estrus in Peranakan Etawa goats. Five healthy non-pregnant goats weighing 20-30 kg, were synchronized using PGF2α (7.5 mg Dinoprost, Enzaprost) intramuscularly twice with 11 days interval. Vaginal cytology examination began the day after the second PGF2α injection. The heat detector (Draminski®, DramiÅ„ski SA, Poland) is an estrus detecting device based on the amount of electric current flowing through the vaginal mucus. Spearman correlation analysis showed a relationship (p <0.05) between heat detector reading and estrus detection based on vaginal cytology. The heat detector readings were very significantly correlated positively (p <0.01) with the number of epithelial cells (coefficient correlation, r= 0.564), but negatively correlated with the number of cornified cells (r = –0.854), and also significantly correlated positively (p <0.05) to the number of leukocytes (r = 0.487). The estrus stages were significantly correlated positively (p <0.05) only to the number of leukocytes (r = 0.918). Likewise, the number of epithelial cells was highly correlated negatively (p <0.01) only to cornified cells (r = –0,643), and the number of cornified cells was significantly correlated negatively (p <0.05) only to the number of leukocytes (r = –0.472). It could be concluded that the features of the proestrus, estrus and metestrus stages based on the goat vaginal cytology was correlated with the reading results of the heat detector.

Co-Authors Abdul Samik, Abdul Afandik, Nadya Ashila Aftabuddin Rz, Muhammad Agustono, Bodhi Al arif, Mohammad Anam Amaliya, Anny Ani Wijayanti Ardiana, Lutfi Ardiansyah, Kresna Ryan Arini, Mirza Asafaizh Ashari, Lukman Azwani, Nabila Bhaskara, Alfiutha Propha Bimo Aksono Brillianti, Febby Fairy Budi Utomo Chusniati, Sri Dadik Rahardjo, Dadik Damayanti, Tita Darsono, Roesno Deskayanti, Asih Endang Suprihati Erma Safitri Hani Plumeriastuti Hardijanto, Hardijanto Hariadi, Mas'ud Herdiansyah, Akbar Dimas Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Indah Norma Triana, Indah Norma Ismudiono Ismudiono, Ismudiono Khafsah, Khafsah M. Anam Al Arif Mafruchati, Maslichah Meiliza, Nazwa Meydilasari, Nanda Irsa Muhammad Aulia Rahman Nawangwulan, Tantri Nenny Harijani Nidom, Chairul Anwar Nur, Maysarah Oktavia Prastiya, Ragil Angga Pudji Srianto Puspita, Satya Alysa Cahya Putri Nurmalia Damayanti Ratna Damayanti Rimayanti Rizki, Andaru Sabdoningrum, Emy Koestanty Safitri, Ainun Merta Saputro, Amung Logam Sasi, Samira Musa Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistyowati, Deny Sunarso, Agus Supriyadi Suryaningsih, Hastini Suwarno Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Tyasningsih, Wiwiek Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiantoro, Krisna Widjiati Widjiati Widodo, Oky Setyo Wiwik Misaco Yuniarti Wurlina, W Yohana, Nadia Yudistira, Neo Rakka Yunita, Maya Nurwartanti

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