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Journal : Pharmacy Reports

The detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP) in developing country Wibowo, Muhammad Rizky; Harfiani, Erna; Sarmoko, Sarmoko; Nugraha, Yudhi
Pharmacy Reports Vol. 1 No. 1 (2021): Pharmacy Reports
Publisher : Indonesian Young Scientist Group and UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.235 KB) | DOI: 10.51511/pr.9

Abstract

The severe acute respiratory syndrome coronavirus (SARS-CoV-2) has infected the human system resulting in Covid-19, and has spread rapidly worldwide. Therefore, a fast, simple, cost-effective, and accurate detecting tool is required. The standard diagnostic tool of the World Health Organization is the reverse transcription-polymerase chain reaction (RT-PCR). This method detects the presence of viral genetic material in the human body with accurate results. However, it has several limitations in terms of equipment, personnel, duration, and cost. Therefore, a fast, simple, and sensitive alternative detection, is required, one of which is the reverse transcription loop-mediated isothermal amplification (RT-LAMP) that functions under isothermal conditions. This method is battery-driven, hence, easy to move closer to the patient. Conclusively, the RT-LAMP test for SARS CoV-2 diagnosis produces comparable sensitivity to a standard RT-PCR and is more suitable for resource-poor settings, such as rural areas of developing countries.
Co-Authors ., Mashoedojo Abd. Rasyid Syamsuri Abdi Wira Septama Abdul Kolib Adi Sukrisno Agneta Irmarahayu Agus Aulung Aisyah, Ammara Almerveldy Azaria Dohongi Almerveldy Azaria Dohongi Angga Hardiansyah Anisah Anisah Anisah Anisah Aprilia, Citra Ayu Ashfia Desi Rahmalina Aulia Chaerani Aulia Chairani Avliya Quratul Marjan Az Zahra, Fatima Boenga Nurcita Budiman, Dicky Citra Ayu Aprilia Dicky Budiman Dinda Nuraini Hanifah Wahab Dwi Arwandi Yogi Saputra Fachri Razi Fajriati Zulfa Fikriana, Chika Nur Hany Yusmaini Ilmi, Ibnu Malkan Bakhrul Ima Maria Indra Kusuma Kartika Putri Amalia Makkiyah, Feda Anisah Manpan Drajat Maria Selvester Thadeus Md Ikhsan Mokoagow Meiskha Bahar Mila Citrawati Mokoagow, Md Ikhsan Muhammad Reiza Mulyadewi, Amelia Luthfiyah Muttia Amalia Nabilah, Ayi Nely Jumaliah Nugraha, Yudhi Nugrahayu Widyawardani Nurfitri Bustamam Nurhakim, Althaf Dhaifullah Pasaribu, Rini Sarah Pasiak, Taufiq Fredrik Primayanti Nurul Ilmi Pritasari, Ilham Arzy Diana Pritha Maya Savitri Putra Sang Fajar El Harry Putra Sang Fajar El Harry Rahmi, Eldiza Puji Ratna Puspita Ratna Puspita Reiza, Muhammad Retno Yulianti Revina, Rika Ria Maria Theresa Ria Maria Theresa Ryan Herardi Sarmoko Sarmoko Serli Marlina Siska Maydianah Sitinjak, Sahat Anugerah Immanuel Sri Rahayu Sri Wahyuningsih Susanti, Febriyolla Sutarto, Riyadi Taufiq Fredrik Pasiak Taufiq Fredrik Pasiak Taufiq Fredrik Pasiak Taufiq Fredrik Pasiak Taufiq Pasiak Taupan Ichsan Tuarita Theodora Putri Simanjaya Tresnawati, Nden Ajeng Ufi Dewintera Uswatun Hasanah Uswatun Hasanah Uswatun Hasanah Utami Wahyuningsih Wahab, Dinda Nuraini Hanifah Wibowo, Muhammad Rizky Wibowo, Putri Larasati Widianingsih Widianingsih Yanti Harjono Hadiwiardjo Yanto Sandy Tjang Yudivaniel Zihono Zihono, Yudivaniel