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Departemen Akuakultur, Universitas Diponegoro Jl. Prof. Soedharto S.H., Semarang 50275, Indonesia, Telp: +62821 5350 5993, Fax: 0247474698
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Pengaruh Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila Syahida, Intan Eska Amalia; Sarjito, Sarjito; Prayitno, Slamet Budi; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

The study aimed was to know the influence of Piper crocatum exstract on the blood profile included total erythrocyte, total leukocyte, differential leukocyte (DL), phagocytosis indeks (IF) and survival rate of common carp (Cyprinus carpio) infected by Aeromonas hydrophila and the best concentration of P. crocatum for imunostimulatory common carp of infected A. hydrophila. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The methods used in this research is experimental methods by using random design complete (RAL) with 4 treatments and three replicates. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The treatments of different addition of P. crocatum exstract on fish feed, 0 gr/kg feed (treatment A), 5 gr/kg feed (treatment B), 10 gr/kg feed (treatment C), 15 gr/kg feed (treatment D). The treatnent was given 21 days. The research results obtained additional P. crocatum exstract significantly different effect (P0,05) on percentage of netrofil (2%) and survival rate of common carp (C. carpio) infected by A. hydrophila (40%), the best concentration of P. crocatum on feed for immunostimulant common carp of infected A. hydrophila, the result showed the best dose 5 g/kg feed
PENGARUH PENAMBAHAN SERBUK LIDAH BUAYA (Aloe vera) DALAM PAKAN TERHADAP KELULUSHIDUPAN DAN PROFIL DARAH IKAN MAS (Cyprinus carpio) YANG DIINFEKSI BAKTERI “Aeromonas hydrophila” Arindita, Chyntia; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penelitian ini bertujuan untuk mengkaji pengaruh penambahan serbuk lidah buaya (Aloe vera) dalam pakan terhadap kelulushidupan dan profil darah ikan mas pasca infeksi bakteri Aeromonas hydrophila serta mengetahui penambahan dosis serbuk lidah buaya yang terbaik. Ikan uji yang digunakan adalah ikan mas (Cyprinus carpio) berukuran 8,55±0,71 cm dan bobot 10,18±2,24 g. Metode penelitian ini adalah eksperimen dengan 4 perlakuan dan 3 ulangan yaitu perlakuan A (0 g/kg pakan), B (30 g/kg pakan), C (60 g/kg pakan) dan D (90 g/kg pakan). Pemberian pakan selama 14 hari sebelum uji tantang. Uji tantang dilakukan dengan menyuntikkan suspensi A. hydrophila dengan dosis 107 sel/mm3 sebanyak 0,1 mL secara intramuscular. Pengamatan dilakukan selama 10 hari pasca infeksi A. hydrophila, pengamatan tersebut meliputi gejala klinis, kelulushidupan dan profil darah. Hasil penelitian menunjukkan bahwa gejala klinis ikan pasca infeksi diantaranya berenang tidak normal (lamban atau vertikal), respon makan menurun, produksi lendir berlebih, peradangan, timbul ulcer, sirip punggung geripis, sisik lepas, daging rusak. Total eritrosit hari ke-4 pasca infeksi mengalami penurunan disemua perlakuan. Total leukosit ikan mas pada hari ke-1 pasca infeksi semakin meningkat. Kadar hematokrit pasca infeksi pada perlakuan B, C dan D lebih tinggi dibandingkan dengan perlakuan A. Kesimpulan yang diperoleh adalah penambahan serbuk lidah buaya memberikan pengaruh sangat nyata terhadap kelulushidupan ikan mas pasca infeksi bakteri, penambahan serbuk lidah buaya dalam pakan dapat meningkatkan nilai total leukosit, eritrosit dan hematokrit lebih banyak dibandingkan dengan ikan yang tidak diberi pakan dengan tambahan serbuk lidah buaya dan dosis serbuk lidah buaya 30 g/kg pakan merupakan dosis terbaik. The aims of this research was to determine the effect of Aloe vera powder in the feed to againts survival rate and blood profile of carp that infected by Aeromonas hydrophila  and to know the addition of A. vera powder dose is best. The fish samples used were carp (C. carpio) with 8.55 ± 0.71 cm lenght and the average weight are 10.18 ± 2.24 g. The method in this research was experimental with 4 treatments and 3 replications. The treatment were A (0 g/kg of feed), B (30 g/kg of feed), C (60 g/kg of feed) and D (90 g/kg feed) and feeds for 14 days. Challenge test was done by injecting a suspension of A.hydrophila at a dose of 107 cells/mm3 as much as 0.1 mL intramuscularly. The observations were done at 10th days post-infection by A. hydrophila, these observations clinical symptoms, survival and include blood profile. The results showed clinical sign experienced after infected  that fishes were swimming in a slant condition (whirling), decreased feed response, inflammation, ulcers arise,necrosis, and scales loose. The results showed that the total erythrocytes at  4th days decrease in all treatments. Total leukocyte carp at the 1st day increased. Total hematocrit in treatment, B, C and D post-infection were higher than treatment A. The conclusion of this research highly significant effect (P > 0.05) to survival rate carp infected by  A. hydrophila and A. vera powder addition in feed able to increase the total erythrocytes, leukoist and total value compared with more hematokrit fish not given feed with the addition of A. vera powder and were dose of A. vera powder 30 g/kg feed gave a better survival rate of carp infected by bacteria A. hydrophila.
KARAKTERISASI DAN UJI POSTULAT KOCH BAKTERI GENUS VIBRIO YANG BERASAL DARI MEDIA KULTUR MASSAL MIKROALGA Rahmanto, Setyo Putro; Sarjito, -; Chilmawati, Diana
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Vibriosis adalah salah satu jenis penyakit yang disebabkan oleh bakteri Vibrio sp. Penyakit ini merupakan salah satu kendala utama yang sering menyerang pembenihan maupun pembesaran udang. Penelitian ini bertujuan mengetahui agensia penyebab vibriosis yang berasal dari media kultur mikroalga dan gejala klinisnya pada udang vaname (Litopenaeus vannamei). Metode yang digunakan penelitian ini adalah metode eksploratif. Metode pengambilan sampel menggunakan metode simple random sampling. Isolasi bakteri menggunakan media Thiosulphate Citrate Bile Salt Agar (TCBSA). Isolat dilakukan seleksi berdasarkan morfologi koloni untuk dilakukan uji postulat koch.  Udang vaname yang digunakan sebagai hewan uji untuk uji postulat koch adalah udang sehat dengan berat 1-1,5 g sebanyak 10 ekor untuk masing – masing isolat dengan ulangan sebanyak 3 kali. Penyuntikan dilakukan pada ruas abdomen kedua dengan kepadatan bakteri 108 CFU/mL dengan dosis 0,1 mL. Pengamatan gejala klinis uji postulat koch dilakukan selama 96 jam. Identifikasi bakteri dilakukan dengan kriteria uji biokimia dan morfologi bakteri. Hasil penelitian didapatkan 21 isolat bakteri. Seleksi bedasarkan morfologi koloni bakteri didapatkan 6 isolat bakteri (TDS10, TDS12, TDS13, TDS15, TDS20, dan TDS9) untuk dilakukan uji postulat koch. Hasil identifikasi bakteri keenam isolat tersebut teridentifikasi sebagai Vibrio harveyi, V. parahaemolyticus, V. alginolyticus, V. fischeri dan V. mimicus yang berpotensi sebagai agensia penyebab vibriosis. Vibriosis was one type of disease caused by genus Vibrio. This disease was one of the major problems in shrimp farming esspecially shrimp hatchery and rearing. Aims of this research to determine the cause of vibriosis derived on culture of microalgae and clinical sign vaname shrimp (Penaeus vannamei) affected by vibriosis. The method in this research used was exploratory research .The sampling method using was simple random sampling method . The isolation of bacteria used Thiosulphate Citrate Bile Salt Agar (TCBSA). Isolate the selection was conducted based on colony morphology for Postulat Koch 's test. Shrimp test used vaname for Postulat Koch 's test was healthy shrimp with weigh of 1-1.5 as 10 shrimps for every repilcation. The bacterial was injected on second abdominal segment with bacterial density of 108 CFU / mL and 0.1 mL volume. The observations of clinical sign for 96 hour after Postulate Koch’s test. Identification bacteria was carried by biochemical and morphological criterias test. The results were obtained 21 isolates. Selection was done based on bacterial colony morphology of bacterial isolates was obtained 6 isaolates (TDS10, TDS12, TDS13, TDS15, TDS20 and TDS9) these isolates was continue for postulates koch's test. The results of identification six bacterial isolates was identified as Vibrio harveyi, V. parahaemolyticus, V. alginolyticus, V. fischeri and V. mimicus as an agent potentially cause vibriosis
PENGARUH PENGGUNAAN EKSTRAK DAUN JERUJU (Acanthus ilicifolius) TERHADAP KELULUSHIDUPAN IKAN KERAPU MACAN (Epinephelus fuscogutattus) YANG DIINFEKSI Vibrio alginolyticus Aonullah, Asep Akmal; Prayitno, Slamet Budi; -, Sarjito
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

ABSTRAK Kendala utama budidaya ikan kerapu adalah tingginya tingkat kematian terutama pada benih sebagai akibat serangan bakteri dan patogen. Penelitian ini bertujuan untuk mengkaji pengaruh penggunaan ekstrak daun jeruju (Acanthus ilicifolius) terhadap kelangsungan hidup ikan kerapu macan  (Epinephelus fuscogutattus) yang diinfeksi Vibrio alginolyticus. Ikan kerapu macan (E. fuscogutattus) sebanyak 120 ekor diberikan perlakuan selama 40 hari dengan pemberian pakan yang ditambahkan ekstrak daun jeruju (A. ilicifolius). Uji tantang dilakukan dengan menyuntikan suspensi bakteri V. alginolyticus secara intramuskular. Prosedur pengamatan dilakukan selama 10 hari pascainfeksi meliputi gejala klinis, kelulushidupan serta gambaran darah ikan kerapu macan. Hasil penelitian menunjukkan gejala klinis ikan sakit diantaranya bergerak lamban, berenang tidak teratur (erratic swimming), berenang di permukaan, warna tubuh ikan menjadi gelap, timbulnya luka di bagian punggung serta geripis pada bagian sirip ikan dan pembengkakan pada bola mata (exopthalmia). Pemberian ekstrak daun A. ilicifolius pada pakan menunjukkan hasil tidak berpengaruh nyata (P>0,05) terhadap kelulushidupan dan jumlah total eritrosit ikan kerapu macan pascainfeksi, akan tetapi penambahan ekstrak daun A. ilicifolius menunjukkan hasil berpengaruh nyata (P<0,05) pada hari ke-8 dengan nilai tertinggi 9,39x104 sel/mm3 serta berpengaruh sangat nyata (P<0,01) terhadap aktifitas fagositik hari ke-4 dengan nilai tertinggi 65%. Dosis terbaik dalam penelitian ini adalah penambahan ekstrak daun A. ilicifolius sebanyak 5 gr/kg pakan. ABSTRACT This research was aimed to find out the effect of using leaf extract Acanthus ilicifolius toward survival rate of tiger grouper (Epinephelus  fuscogutattus) infected by Vibrio alginolyticus. 120 fish E. fuscogutattus was treated 40 days with the added leaf extract A. ilicifolius in the feeding process. The test was done by injecting of bacterial suspensions V. alginolyticus in the intra-muscular of the fish. Observation procedure was performed for 10 days after the infection such as clinical symptoms, survival rate and blood picture of the fish. The results showed clinical symptoms of sick fishes i.e. abnormally swiming activity, erratic swimming, swimming on the surface, dark colouration of the skin, a wound on the back and fins, and exopthalmia. The test results showed that extract of A. ilicifolius leaf not significantly different (P>0.05) on survival rate and total erythrocyte of E. fuscogutattus post-infection, but the addition of leaf extracts A. ilicifolius indicate results significantly different (P<0.05) on day 8 with the highest score of 9.39 x104 cells/mm3 and differed significantly (P<0.01) on  phagocytic activity day 4 with a highest score of 65%. The best dosage in this research was 5 gr/kg of feed.
KEANEKARAGAMAN AGENSIA PENYEBAB VIBRIOSIS PADA UDANG VANAME (Litopenaeus vannamei) DAN SENSITIVITASNYA TERHADAP ANTIBIOTIK Apriliani, Milza; Sarjito, -; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Udang vaname (Litopenaeus vannamei) merupakan salah satu jenis udang ekonomis penting di Indonesia. Serangan vibriosis adalah salah satu kendala pada kegiatan budidaya udang vaname. Penelitian ini bertujuan untuk mengetahui agensia penyebab vibriosis yang menginfeksi udang vaname di Tambak Intensif Desa Wonorejo, Kendal, sensitivitasnya terhadap antibiotik eritromisin, enrofoloksasin, dan oksitetrasiklin, serta gejala klinis udang vaname yang terserang vibriosis. Metode pada penelitian ini adalah metode eksploratif konfirmatori dan metode pengambilan sampel menggunakan metode purposive sampling. Bakteri Vibrio diisolasi dari bagian hepatopankreas dan ekor pada media Thiosulphate Citrate Bile Salt Agar (TCBSA). Berdasarkan karakteristik morfologi terdapat 37 isolat yang diperoleh, kemudian dilakukan identifikasi secara biokimia sehingga didapatkan 5 jenis bakteri Vibrio. Kelima jenis bakteri Vibrio dilakukan uji sensitivitas dan uji postulat Koch. Udang vaname yang digunakan dalam uji postulat Koch memiliki panjang tubuh 10-12 cm yang disuntik dengan kelima isolat bakteri dengan konsentrasi 106 CFU/mL. Antibiotik yang digunakan dalam uji sensitivitas adalah eritromisin, enrofoloksasin, dan oksitetrasiklin dengan dosis 35µg. Bakteri Vibrio sp., berasarkan identifikasi secara biokimia adalah V. parahaemolyticus, V. harveyi, V. fluvialis, V. mimicus, dan V. vulnificus. Hasil uji sensitivitas menunjukkan bahwa kelima isolat bakteri resisten terhadap antibiotik eritromisin, enrofoloksasin, dan oksitetrasiklin. Udang vaname yang terserang vibriosis di Tambak Intensif Desa Wonorejo, Kendal serupa dengan gejala klinis yang ditunjukkan udang vaname pada uji Postulat Koch yaitu hepatopankreas kecoklatan, uropoda kemerahan, pereopoda kemerahan, pleopoda kemerahan, nekrosis pada antennal scale dan berwarna kemerahan, serta terdapat melanosis pada abdomen. Hasil uji postulat Koch menunjukkan bahwa kelima isolat bakteri mengakibatkan udang vaname sakit engan gejala klinis sama dengan udang vaname sampel dan menimbulkan kematian  92% - 100% selama 14 hari masa pemeliharaan.Vaname shrimp is one of shrimp species that has an important economic value in Indonesia. The vibriosis desease is on of the constraints on vaname shrimp aquaculture activities. The purposes of this research are to discover clinical signs and vibriosis causative agent that infected vaname shrimp farmed intensively in the village of Wonorejo Kendal Regency as well as its sensitivity against antibiotics erythromycin, enrofoloxacin, and oxytetracycline. The methods of this research are explorative confirmatory method and sampling method using purposive sampling method. Vibrio bacterium is isolated from hepatopancreas and tail on Thiosulphate Citrate Bile Salt Agar media (TCBSA). Based on morfology characteristic, there are 37 isolat, which are obtained and then identified biochemically, therefore they result 5 types of Vibrio sp., bacterium. After that, the 5 of Vibrio sp., bacterium is performed sensitivity test and Koch’s postulates test. The length of vaname shrimp’s body that is used during the Koch’s postulates test is 10-12 cm and then injected by the 5 of isolat bacterium with concentration 106 CFU/mL. The antibiotic that is used during the sensitivity test are erythromycin, enrofoloxacin, and oxytetracycline with the dose 35µg. Vibrio bacterium based on the biochemical test are V. parahaemolyticus, V. harveyi, V. fluvialis, V. mimicus, dan V. vulnificus. The result of sensitivity test shows that the five of isolates bacterium are resistant against antibiotics erythromycin, enrofoloxacin, and oxytetracycline. Clinical signs that is shown by vaname shrimp farmed intensively in the village of Wonorejo Kendal Regency which contaminated vibriosis and Koch’s postulates test are browny on hepatopankreas, redish on uropoda, pereopoda and pleopoda, redish and necrosis on antennal scale, and melanosis on abdomen. The Koch’s postulates result shows the five of isolat bacterium cause vaname shrimp get sick with similar clinical signs to vaname shrimp sample and they result on high mortality ≥ 92% during 14 days of maintenance.
Pengaruh Perendaman Ekstrak Daun Belimbing Wuluh (Avverhoa bilimbi Linn) terhadap Kelulushidupan dan Histopatologi Hati Ikan Gurami (Osphronemus gouramy) yang Diinfeksi “Aeromonas hydrophila” Nineung, Ditha Febriana Dewanti; Sarjito, -; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 4, Nomor 1, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Bakteri A. hydrophila merupakan penyebab penyakit Motil Aeromonas Septicemia pada ikan gurami. Salah satu bahan alami yang berpotensi  untuk pengobatan penyakit aeromonas adalah daun belimbing wuluh (A. bilimbi L.). Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak daun belimbing wuluh dan mengetahui dosis terbaik terhadap kelulushidupan dan histopatologi hati ikan gurami. Metode penelitian yang digunakan adalah metode eksperimental, dengan rancangan percobaan  menggunakan Rancangan Acak Lengkap (RAL) yaitu 4 perlakuan dan 3 ulangan. Uji in vitro dilakukan sebelum penelitian, dalam rangka penentuan dosis untuk uji in vivo. Perlakuan yang digunakan diantaranya perlakuan A (dosis 0 ppm), perlakuan B (dosis 1000 ppm), perlakuan C (dosis 2000 ppm), dan perlakuan D (dosis 3000 ppm). Ikan gurami terlebih dahulu diinjeksi A. hydrophila dengan cara menyuntikkan 0,1 mL bakteri sebanyak 107CFU/mL secara intramuskular. Setelah ikan gurami menunjukkan gejala klinis, maka dilakukan perendaman dengan ekstrak daun belimbing wuluh selama 20 menit dengan 2 kali perendaman, yaitu pada hari pertama pasca injeksi dan hari ke 6 pasca perendaman pertama. Hasil penelitian diperoleh, bahwa perendaman ekstrak daun belimbing wuluh memberikan pengaruh yang berbeda nyata (p<0,05) terhadap kelulushidupan ikan gurami. Kelulushidupan tertinggi pada perlakuan D (56,67%±15,28), kemudian perlakuan C (43,33%±11,55), diikuti perlakuan B (30,00%±24,46), dan yang paling rendah pada perlakuan A (6,67%±11,55). Hasil pengamatan histopatologi hati menunjukkan kelainan berupa nekrosis, degenerasi, melanomakrofag dan kongesti. Hasil penelitian diperoleh bahwa dosis 3000 ppm merupakan dosis terbaik untuk pengobatan penyakit Motil Aeromonas Septicemia pada ikan gurami dengan metode perendaman. A. hydrophila is one of the causes Motil Aeromonas Septicemia disease in gouramy (O. gouramy). One of the natural materials to cure aeromonas disease is starfruit leaves (A. bilimbi L.). The aims of this research were to determine an effect by using starfruit leaves (A. bilimbi L.) and to determine the best dose to survival rate and Histopathology of gouramy (O. gouramy) liver. Research methods used experimental method and experimental design used Completely Randomized Design with 4 treatments and 3 replications. In vitro test was done before getting research to determine dose for in vivo test.  The treatments were treatment A (0 ppm), treatment B (1000 ppm), treatment C (2000 ppm), and treatment D (3000 ppm). Previously, gouramy (O. gouramy) was injected by 0,1 ml A. hydrophila intramuscurally with bacteria density 107 CFU/ml. After carps showed clinical sign, so the immersion was done by starfruit leaves (A. bilimbi L.) extract for 20 minutes with 2 replications, in first day of post-injection and day sixth of first immersion. Results of this research were immersion of starfruit leaves extract gaves a significantly different effect (p<0.05) to gouramy (O. gouramy) survival rate. Other results of this research were the highest survival rate was at treatment D (56.67%±15.28), then treatment C (43.33%±11.55),  followed with treatment B (30.00%±24.46), and the lowest was treatment A (6.67%±11.55). Observation of liver Histopathology showed abnormalities such as necrosis, degeneration, melanomacrofag, and congestion.  Based on survival rate, consentration 3000 ppm was the best dose to cure motil aeromonas septicemia in gouramy (O. gouramy) with immersion method.
PENGARUH PENAMBAHAN EKSTRAK JAHE MERAH (Zingiber officinale var. Rubrum) PADA MEDIA PEMELIHARAAN TERHADAP KELULUSHIDUPAN DAN PERTUMBUHAN IKAN GURAMI (Osphronemus gouramy) YANG DIINFEKSI BAKTERI Edwardsiella tarda Prastiti, Linuwih Aluh; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 4, Nomor 3, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Gurami (O. gouramy) adalah salah satu jenis ikan air tawar yang dipilih pembudidaya untuk dipelihara. Namun kendala yang sering dialami dalam membudidayakan kultivan ini adalah serangan penyakit yang disebabkan oleh bakteri E. tarda. Penggunaan antibiotik dalam jangka panjang dapat berdampak negatif, diantaranya dapat menimbulkan resisten terhadap bakteri dan dapat mencemari lingkungan. Penggunaan bahan alami mulai berkembang untuk pengobatan ikan yang terserang penyakit, salah satu bahan alami yang digunakan yakni ekstrak jahe merah (Z.officinale var. Rubrum). Penelitian ini bertujuan untuk mengetahui gejala klinis, pertumbuhan, serta pengaruh perendaman ekstak jahe merah sebagai terhadap kelulushidupan ikan gurami yang diinfeksi bakteri E. tarda. Ikan gurami yang digunakan sebanyak 120 ekor dengan ukuran 7 - 9 cm dan diinfeksi bakteri E. tarda dengan kepadatan 108 CFU/ml secara intramuskular. Perendaman ekstrak jahe merah dilakukan pada hari ke 3 pasca infeksi setelah muncul gejala klinis seperti timbulnya luka (ulcer), pendarahan (haemorhagic), dropsy dan geripis pada ekor, sirip dada dan punggung. Perendaman ekstrak jahe merah berpengaruh nyata (P<0,05) terhadap kelulushidupan ikan gurami yang diinfeksi bakteri E. tarda. Nilai rata – rata kelulushidupan terendah hingga tertinggi berturut – turut yaitu 3,33% (perlakuan A), 26,67% (perlakuan B), 40,00% (perlakuan C) dan 56,67% (perlakuan D). Gourami (O. gouramy) is one of freshwater fish that has choosen as cultured fish by farmers. However, there are problems in culturing this cultivan, one of them is disease caused by E. tarda. Long-term use of antibiotics have a negative impact, which could cause resistance to bacteria and contaminate environment. Red ginger extract (Z. officinale var. Rubrum) as natural ingredients could be used as alternative treatment for this disease. This research aimed to determine clinical sign, growth rate and effect of short bathing of red ginger extract on survival rate of gourami that were infected with E. tarda bacteria. Gourami used were 120 fish with 7 - 9 cm in size and infected with E. tarda with density of 108CFU/ml intramuscularly. Immersion in red ginger extract was conducted on 3rd day post infection after the clinical sign was showed such as ulcer, haemorhage, dropsy, and erosion at caudal, pectoral and dorsal fin. Short bathing in red ginger extract significantly (P <0.05) affected the survival rate of gourami were infected with E. tarda but did not affect growth rate. Average values of the survival rate respectively are 3,33% (treatment A), 26.67% (treatment B), 40.00% (treatment C) and 56.67% (treatment D).
Aplikasi Vaksin Dna Koi Herpes Virus (Khv) Melalui Metode Perendaman Dengan Dosis Yang Berbeda Dalam Upaya Pencegahan Penyakit Pada Ikan Mas (Cyprinus carpio) Chairunnisa, Sekar Ayu; Prayitno, Slamet Budi; -, Sarjito; Santika, Ayi; Nuryati, Sri
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

The purpose of this research was to determine fish clinical signs of KHV-infected and determine effectiveness of KHV DNA vaccine with immersion method to prevent koi herpes virus (KHV) infection. This research was conducted in National Center for Freshwater Aquaculture Development (BBPBAT) Sukabumi, from October 2012 to Februari 2013. The fish samples used were carp seed free KHV with size 3 – 5 cm as many as 450 animals. Five (5) treatments and 3 replications were administered in this research. The treatment were, without vaccine, vaccine at dose of 104, 105, 106, and 107 CFU/ml. Vaccination technique used was vaccination through immersion method. One of the vaccine that has been developed was KHV DNA vaccine. Eksperimental fishes were maintain for 30 days past vaccination, and then challenged with KHV and observed for 21 days. The variables measured were clinical signs and survival rate. The results of this research indicated that non vaccinated fish (A), vaccinated fish at 104(B) and 105(C). demonstered clinical signs of KHV-infected fish, while vaccinated fish at 106(D) and 107(D) visibly normal. This results indicated that vaccination using KHV DNA vaccines at 106 and 107 CFU/ml could prevent eksperimental fish from KHV-infection. PCR results showed that fish with treatments A, B and C positive infected KHV, while treatment D and E negative KHV. Survival rate (SR) at treatment A (15,56%), B (50,00%), C (53,33%), D (78,89%) and E (83,33%). For prevention of KHV, dose vaccine DNA KHV 106 and 107 CFU/ml could be used. This results supported by survival rate from treatment D (78,89%) and E (83,33%) higher than treatment A, B and C.
Pengaruh Ekstrak Daun Sirsak (Annona muricata) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila Nurjannah, R. Dewi Dharina; Prayitno, Slamet Budi; Sarjito, Sarjito; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Carp is a freshwater species that is easily cultivated. One of the constraints in cultivating carps (C. carpio) is diseases that ultimately caused mass mortality. The disease that generally affects carp is Motile Aeromonas Septicemia (MAS) caused by Aeromonas hydrophila. Soursop leaves is one of the herbs that can be used as an ingredient to control fish diseases because it contains chemicals that acts bacteriocide. The purpose of this research was to determine the influence and the best dose of soursop leaf extract in feed to the blood profile and survival of carp infected by A. hydrophila. The treatments of this research were addition of soursop leaf extract on feed with 4 different dosages and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet) and D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B (5 g/kg diet) significantly different (P0.05) on total erythrocyte (0.83×106 cells/mm3), differential leukocyte include the percentage of monocytes (7.67%), neutrophils (8.67%), lymphocytes (83.67%), phagocytic activity (37%) and survival rate (50%). It can be conclude that soursop leaf extract at dosage 5g/kg was able to stimulate immune response of carp, it was characterized by an increased total leukocytes of carp infected by A. hydrophila.
Inventarisasi Bakteri yang Berpotensi sebagai Probiotik dari Usus Ikan Bandeng (Chanos chanos) Wardani, Briliani Ayu; Sari, Rohita; -, Sarjito
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Abstrak Ikan bandeng tahan penyakit karena didalam tubuhnya terdapat bakteri yang berasosiasi membantu pencernaan untuk peningkatan kesehatan dan mencegah penyakit pada ikan bandeng. Mekanisme pertahanan terhadap penyakit diduga mampu menghambat bakteri pathogen. Kasus penyakit pada ikan bandeng selama ini jarang ditemukan, oleh karena itu ikan bandeng diduga mempunyai kemampuan immune system yang tinggi. Untuk mencari bakteri kandidat probiotik yaitu dengan cara skrining bakteri yang terdapat pada usus ikan bandeng untuk mendapatkan jenis-jenis bakteri yang dapat dikembangkan sebagai pembentukan konsorsium probiotik. Tujuan dari penelitian adalah untuk menginventarisasi bakteri pada usus ikan bandeng yang berpotensi sebagai bakteri kandidat probiotik pada usus ikan bandeng (Chanos chanos). Metode yang digunakan adalah eksplorasi. Prosedur penelitian yaitu skrining bakteri yang berpotensi sebagai kandidat probiotik meliputi isolasi bakteri, pemurnian isolat, uji sensitivitas, isolat terpilih, uji morfologi dan biokimia. Uji morfologi yang dilakukan adalah pengamatan bentuk sel, warna koloni, dan bentuk koloni. Selanjutnya dilakukan uji biokimia yaitu uji gram, uji oksidatif-fermentatif, uji motilitas, uji aerob-anaerob, uji katalase, uji oksidase, dan uji gula. Hasil penelitian menunjukkan bahwa dari enam belas isolat bakteri, diperoleh empat isolat bakteri (JA1, JA3, JA4, dan JA7) berpotensi sebagai kandidat probiotik untuk dapat dikembangkan sebagai pembentukan konsorsium probiotik. Berdasarkan karakterisasi secara morfologi dan biokimia menunjukkan bahwa isolat JA1 memiliki kekerabatan terdekat dengan Lactobacillus fermentum (86%), isolat JA3 dengan Lactobacillus gasseri (91%), isolat JA4 dengan Lactobacillus delbrueckii (86%) dan isolat JA 7 dengan Micrococcus lylae (93%). Dari hasil penelitian ini dapat disimpulkan bahwa bakteri kandidat probiotik yang terdapat pada saluran pencernaan ikan bandeng (Chanos chanos) adalah Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus delbrueckii, dan Micrococcus lylae. Abstract Milkfish includes disease resistant fish this is due to gut microflora that maintain health and prevent milkfish from disease infection. Defense mechanism against disease is suspected to prevent pathogenic bacteria. The cases of the disease in milkfish have been rarely found, therefore milkfish thought to have high immune system ability shown by the presence of bacteria that potentially probiotic bacteria. To search the candidate probiotics that is by screening of bacteria found in the intestine of milkfish to obtain the types of bacteria that can be developed as the formation of probiotics consortium. The purpose of this research was to inventory the bacteria in the intestine of milkfish (Chanos chanos) that is potential as probiotic bacteria. The method used is exploration. Procedure of the research, includes the isolation of bacterial purification, test sensitivity, candidate probiotic isolate, morphological and biochemical tests. Tests were conducted morphological observation, ic, cell shape, colony color, and form colonies. Further biochemical tests are gram test, oxidative-fermentative test, motility test, aerobic-anaerobic test, catalase test, oxidase test, and test of sugar. The results showed that four (JA1, JA3, JA4, and JA7) of the sixteen isolates as probiotic candidate. The four isolates was potentially to develop the formation of probiotics consortium. Based on morphological and biochemical characterization showed that JA1, JA3, JA4, and JA7 isolates were closely related to Lactobacillus fermentum (86%), Lactobacillus gasseri (91%), Lactobacillus delbrueckii (86%) and Micrococcus lylae (93%) respectively. From this research it could be concluded that the candidate probiotic bacteria found in the intestine of milkfish (Chanos chanos) was Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus delbrueckii, and Micrococcus lylae.
Co-Authors - Aminah - Desrina, - - Istikhanah - Susanti, - - Triyaningsih A. Harjuno Condro Haditomo A. Santoso Adhi Kurniawan Adi Santoso Afifah, Roidah Nur Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agus Dwi Anggono Agus Pranoto Syah, Agus Pranoto Agus Yulianto Aji, Nisa Pamesty Rahma Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Alfia Magfirona Alfiyani, Lina Ali Djunaedi Amalia, Ayu Rizki Amanda Mega Putri, Amanda Mega ANGELA MARIANA LUSIASTUTI Anggoro, Agung Doni Anggun Putriani Situmorang, Anggun Putriani Aninditia Sabdaningsih Anisa Dwiaryani Latifah Annisa Oktafianti Nurlatifah, Annisa Oktafianti Aprilia Dwi Indriani Arifin Arifin Arum Almuaromah, Dita Asep Akmal Aonullah Astri Pujiati Aulia Resty Wijayanti Aulia, Annisa Syahida Ayi Santika Ayu Wulandari Bosma, Roel Bosma, Roel H. Briliani Ayu Wardani Budi Setiawan Buyung Junaidin, Buyung Caesa, Genio Chairulina Pitrianingsih CHONDRORESMI BANOR FAWWAZ Chyntia Arindita Dani Indrarini David Panca Wijaya, David Panca Desrina Desrina Dewi Nurhayati Dhani Mutiari Dian Ratna Sari Dian Wijayanto Diana Chilmawati Diana Chilmawati Diana Rachmawati Dicky Harwanto Ditha Cahyaningrum Ditha Febriana Dewanti Nineung Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Ervia Yudiati Fajar Basuki Famelia Meta Putri Fandy Malik Muhammad, Fandy Malik Fatian, Adella Spextania Ferdian Bagus Feriandika Fifiana Zulaekah Fitriadi, Ren Gina Saptiani Haeruddin Haeruddin Hasna, Salma Khoironnida Hasyim Asyari Ika Puspitasari Indah Febry Hastari Intan Eska Amalia Syahida Istiyanto Samidjan Jery, Jery Johannes Hutabarat Jokosisworo Jokosisworo Kewa, Kristofora Karolina Khuzaimah, Ima Siti Kurniawan Kurniawan Laksono Trisnantoro Lestari Lakhsmi Widowati Lilik Maslukah Lilik Setiyaningsih Linuwih Aluh Prastiti Lukman Anugrah Agung Lukman Lukman Marwenni Siregar, Marwenni Milza Apriliani, Milza Mita Umiliana, Mita Monica Nanda Muchtar muchtar Muhammad Burhan Mukhlisin, Latutik Nailil Muna Nida Qolbi Salma Rochani Noor Alis Setiyadi Nur Aklis Nur Annisa Nuri Nia Yanti, Nuri Nia Nurul Hidayati Ocky Karna Radjasa Panji Yusroni Anwar Prabowo, Anggit Bayu Pramudita Apriliyanti Prayitno, S. Budi Pungki Nanda Pratama Purwanto Purwanto Pusaka, Semerdanta R. Dewi Dharina Nurjannah Rahman, Nuril Endi Rahmawati, Amelia Rahmi Gusti Darma Raynol Simorangkir Rensiga Rintan Bunga Sari Restiana Ariyati Restiana Wisnu Ariyati Rini, Endah Setyo Ristiawan Agung Nugroho Rohita Sari Rosa Amalia Rosalina Safitri Rusydina Qamarul Salikin S. Budi Prayitno Sahala Hutabarat Sarastiti, Siwi Schrama, Johan Sekar Ayu Chairunnisa Seto Windarto Setyo Putro Rahmanto Setyowati, Suryaning Siti Nurjanah Siti Ziyadaturrohmah Siwi Hartanti Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Soedibya, Petrus Hary Tjahja Sri Hastuti Sri Hastuti Sri Nurchayati Sri Nuryati Sri Rejeki Subagiyo Subagiyo Subandiyono Subandiyono Subroto Subroto Sulisyaningrum Sulisyaningrum Sumini Sumini, Sumini Suminto , Suminto - Suminto Suminto Suminto Suminto Suminto Suminto Suminto Suminto Suradi Wijaya Saputra Tita Elfitasari Titik Susilowati Tri Mulyadi Trienes, Yoni W. Widiatmoko Wiji Utami, Wiji Wijianto Wijianto Wisnu Widyantoro Wiyadi Yelliana Fatmawati Suwarno Yohanes Kristiawan Artanto