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All Journal Maspari Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Rotasi Jurnal Pasir Laut Journal of Aquaculture Management and Technology Media Mesin: Majalah Teknik Mesin Jurnal Kelautan Tropis Omni-Akuatika Turbo : Jurnal Program Studi Teknik Mesin Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Riset Akuakultur Scripta Biologica Journal of Aquaculture and Fish Health PENA AKUATIKA : JURNAL ILMIAH PERIKANAN DAN KELAUTAN Jurnal Media Akuakultur Indonesia Abdi Geomedisains Journal Of Human And Education (JAHE) Warta LPM Journal of Community Services and Engagement: Voice of Community (VOC) Majalah Teknik Mesin

Sarjito -

Departemen Akuakultur, Universitas Diponegoro Jl. Prof. Soedharto S.H., Semarang 50275, Indonesia, Telp: +62821 5350 5993, Fax: 0247474698

Author-ID : 373626

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Identifikasi Dan Uji Postulat Koch Agensia Penyebab Penyakit Bakteri Pada Ikan Lele (Clarias gariepinus) yang Berasal dari Demak Wijayanti, Aulia Resty; -, Sarjito; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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The aim of the research was to find out the clinical sign of the catfish sample that has been infected by the bacteria and to find out the causative agent bacteria diseases of Clarias gariepinus from Demak. The fish sample collection used purposive random sampling method. The observation of clinical sign was implemented toward ten catfish samples. The isolation of bacteria from moribund fish surface and kidney of catfish was conducted by streaking method on NA and GSP media. Based on the characteristic of morphology (colour, form of colony), it was obtained five isolate from eleven isolates of bacteria for the next test. The test of postulat koch toward five isolates of ten test fish and characterized them to causative agent bacterial through morphological and biochemical characterization. The result of the research showed that the clinical sign were detected irregular swimming, balance disorders, fish stand still at the bottom of the aquarium, the fish limp, injury/red blisters at the injection site, a pale body color, porous on tails, fins and antennas. The test results of postulates koch obtained that the five isolates (D7, D10, D12, D14, D16) were capable of causing pain on test fish. Based on the postulate test also showed only two isolates (D7 and D12) were caused mortality of 30% of the test fish. The results of the morphological and biochemical characterization found that the five agents causing disease in fish from Demak were abbreviated namely of Pseudomonas plecoglossicida, Flavobacterium sp., Edwardsiella ictaluri, Aeromonas caviae and Aeromonas caviae, respectively.

Pengaruh Penambahan Tepung Cacing Tanah (Lumbricus rubellus) dalam Pakan Buatan Terhadap Jumlah Total Hemosit dan Aktivitas Fagositosis, Udang Vanname (Litopenaeus vannamei) Pujiati, Astri; -, Sarjito; -, Suminto
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstrak Â Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung cacing tanah terhadap jumlah total hemosit (THC) dan aktivitas fagositosis (AF) pada hemolim udang vannamei, serta mengetahui dosis terbaik. Materi yang digunakan udang vannamedengan berat Â± 8 gram yang dipelihara selam 30 hari. Metode yang digunakan metode eksperimental dengan Â rancangan acak lengkap (RAL) dengan 4 perlakuan, yaitu perlakuan A (tanpa penambahan tepung cacing tanah), perlakuan B (pemberian 40 gr/kg tepung ke pakan), perlakuan C (pemberian 60 gr/kg tepung ke pakan), dan perlakuan D (pemberian 80 gr/kg tepung ke pakan) masing-masing 3 ulangan. Frekuensi pemberian pakan dilakukan 3 kali sehari. Parameter yang diukur yaitu jumlah total hemosit (THC) dan aktivitas fagositosis (AF). Hasil penelitian menujukkan bahwa tepung cacing tanah memberikan pengaruh sangat nyata (P<0,01) terhadap jumlah total hemosit dan aktivitas fagositosis hemolim. Hasil terbaik didapatkan pada hari ke-30 pada perlakuan D (80 gr/kg) yaitu sebesar 45,8x106 sel/mm3, danÂ aktivitas fagositosis pada perlakuan D (80 gr/kg) sebesar 92,6%. Dosis yang terbaikÂ diperoleh pada perlakuan D (80 gr/kg). Â Abstract The aims of this experiment was to find out the effect of earthworm meal of Â total hemocyte (THC) and phagocytic activityÂ hemolim vannamei shrimp. This experiment used vanname shrimp with Â± 8 grams weight and cultured in 30 days. This experiment used was experimental method with complete random design with 4 treatmens, i.e.Â treatment A (without Â meal earthworms addition), treatment B (addition 40 g / kg meal to diet); treatment C (addition 60 g / kg meal to diet), and treatment D (addition 80 g / kg meal to diet) will 3 replications. Feeding frequency apply cared 3 times/day. Measured variables were the total number of hemocytes (THC) and phagocytic activity. The results showed that earthworm mealÂ was highly significant effect (P <0.01) onÂ total hemocytes count (THC) and phagocytic activity hemolim of vanname shrimp. The best dose of earthworm mealâ€™s was 80gr/kg (treatment D).

IDENTIFIKASI MOLEKULER SPESIES BAKTERI KANDIDAT PROBIOTIK YANG DIISOLASI DARI USUS UDANG VANAME (Litopenaeus vannamei) KOLEKSI DARI KABUPATEN SUBANG, JAWA BARAT Sarastiti, Siwi; Suminto, Suminto; Sarjito, Sarjito
Jurnal Pasir Laut Vol 4, No 1 (2020): Februari
Publisher : Magister Manajemen Sumberdaya Perairan, Departemen Sumberdaya Akuatik

Vaname (Litopanaeus vannamei) merupakan salah satu jenis kultivan budidaya udang putih yang bernilai ekonomis penting dan telah dibudidayakan secara intensif. Salah satu permasalahan budidaya secara intensif yaitu adanya penyakit yang disebabkan oleh bakteri patogen. Upaya mencegah serangan penyakit oleh bakteri patogen pada udang adalah dengan pemberian probiotik yang diisolasi dari usus udang vaname. Penelitian ini bersifat eksploratif bertujuan untuk mendapatkan isolat bakteri kandidat probiotik yang terdapat pada usus udang vaname (L. vannamei) dan mengidentifikasi secara molekuler bakteri kandidat probiotik yang terdapat pada usus udang vaname (L. vannamei). Prosedur dalam penelitian ini meliputi pengambilan sampel udang, isolasi bakteri usus udang, uji biokimia, uji aktivitas enzimatik, uji aktivitas antibakteri, identifikasi dan analisis molekuler isolat bakteri dari usus udang vaname. Hasil penelitian yang telah dilakukan, didapatkan 11 isolat bakteri dari usus udang vaname dari daerah Subang, Jawa Barat, memiliki morfologi yang beragam. 6 isolat bakteri positif terhadap ke empat uji proteolitik, 8 selulolitik, 7 lipolitik dan 7 amilolitik. Terdapat 2 isolat (SS5 dan SP3) memiliki aktivitas antibakteri terhadap bakteri V. harveyi dan V. Parahaemolyticus. Berdasarkan identifikasi dan alnalisis molekuler diketahui isolat bakteri dengan kode SS5 memiliki kemiripan 99,93% dengan Bacillus flexus pada nomor akses NR_113800.1.

The Diversity of Causative Agent Associated With Bacterial Diseases on Catfish (Clarias gariepinus) with Molecular Based from Demak, Indonesia Sarjito Sarjito; A. Harjuno Condro Haditomo; Desrina Desrina; Restiana Ariyati; S. Budi Prayitno
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and D14) that were caused 10–55% of fishes get sick and 0–30% fishes mortal. On the other hand, there were 13 isolates do not cause both sick and mortality of fish. On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.

Molecular Characteristics of Indonesian Isolate Enterocytozoon hepatopenaei Based on Sequence Analysis of 18S rRNA Genes Yohanes Kristiawan Artanto; Slamet Budi Prayitno; Sarjito Sarjito; Desrina Desrina; Alfabetian Harjuna Condro Haditomo
Journal Omni-Akuatika Vol 15, No 1 (2019): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

ABSTRACT Enterocytozoon hepatopenaei (EHP) infection have been reported as an obstacle of whiteleg shrimp (Penaeus vannamei) culture in Indonesia. However, understanding of the molecular characteristics of EHP species in Indonesia is not widely known. The aims of this study were to determine the identity and characters of DNA, and their phylogeny of EHP species from several different locations in Indonesia with specific references to 18S rRNA gene. The EHPs were collected from cultured P.vannamei from Lampung, Pangandaran, Sidoarjo, Banyuwangi, Probolinggo, Blitar, Makassar, and Lombok. Thirteen (13) samples were randomly selected to explore their gene characters through 18S rRNA gene sequencing. The primers used were EHP_F and EHP_1R. Parameter observed were DNA sequencing, nucleotide sequence homology with related available genes in the Gen Bank database, multiple sequence alignment, and reconstruction of genetic relationship trees. DNA sequence homology analysis showed that all samples had 99.89-100% similarity to Indian Enterocytozoon hepatopenaei (Accession Number MH259890.1 and MH260592.1). The alignment results illustrated that all EHP sequences of Indonesian isolates were 100% identical each other. The phylogenetic tree topology provided information that all sample accessions were in the same clade and spread evenly. The conclusion were that the Indonesian EHP species were identical (100%) and it could be said that they were genetically homogeneous. Keywords: Enterocytozoon hepatopenaei, 18S rRNA gene, Indonesia.

MODEL ADSORPSI LOGAM BERAT Pb, Cu, dan Zn SISTEM AIR-SEDIMEN MUARA SUNGAI BANJIR KANAL BARAT SEMARANG Lilik Maslukah; Ervia Yudiati; Sarjito .
Maspari Journal : Marine Science Research Vol 9, No 2 (2017): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

Adsorpsi merupakan proses penting dalam mengontrol transfer logam dari fase terlarut menjadi fase padatan. Logam Pb, Cu dan Zn merupakan logam yang dalam konsentrasi tinggi membahayakan bagi lingkungan. Penelitian ini bertujuan untuk memprediksi model adsorpsi Pb, Cu dan Zn dalam sistem air laut-sedimen di Muara Sungai Banjir Kanal Barat, Semarang. Sampel air dan sedimen diambil dari 7 stasiun. Untuk mendapatkan konsentrasi Pb, Cu dan Zn, sampel air diekstraksi menggunakan larutan organic APDC_MIBK. Sedangkan untuk sampel sedimen melalui tahapan destruksi menggunakan asam chlorida (HCl) dan asam nitrat (HNO3) pada suhu 1000C selama 8 jam. Pembacaan nilai absorbansi dilakukan dengan AAS merk Shimidzu tipe 680 AA. Data yang didapat dikalkulasi berdasarkan model adsorpsi yaitu model Partisi, model Freundlich dan model Langmuir. Hasil perhitungan linieritas menunjukkan bahwa transfer ion logam Pb, Cu dan Zn ke dalam sedimen mengikuti model Langmuir dengan koefisien determinasi (R2) 0,72 untuk Pb, 0,96 (Cu) dan 0,73 (Zn). Sementara nilai kapasitas adsorpsi maksimum logam Pb (11,01-25,72), logam Cu (22,88-112,79) dan Zn (216,68-25.106). Kapasitas adsorpsi maksimum antara fase terlarut terhadap material partikel jauh lebih besar dibanding antara sistem terlarut terhadap sedimen.KATA KUNCI: Adsorpsi, Freundlich, Langmuir, model, partisi.

Penggunaan Repetitive Sequence-Based Polychain Reaction (REP-PCR) Untuk Pengelompokan Bakteri Vibrio yang Berasosiasi dengan Ikan Kerapu Sakit dari Perairan Karimunjawa Sarjito Sarjito
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Ikan kerapu sakit diperoleh dari keramba jaring apung yang berlokasi di perairan Karimunjawa. Penelitian ini bertujuan untuk mengkaji penggunaan Repetitive Sequence-Based Polychain Reaction (REP-PCR) untuk pengelompokan bakteri genus vibrio yang berasosiasi dengan berbagai ikan kerapu sakit. Sebanyak 32 isolat Vibrio berhasil diisolasi dari bagian luka maupun ginjal berbagai ikan kerapu sakit dengan medium Thiosulfat Citrat Bile Salt Agar (TCBSA). Hasil rep-PCR diperoleh bahwa terdapat delapan kelompok bakteri vibrio yang berasosiasi dengan ikan kerapu sakit. Oleh karena itu, pada penelitian ini, delapan isolat (JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, dan JT 31) yang masing-masing mewakili kelompoknya akan dilakukan uji selanjutnya. Teknik molekuler gen 16S rDNA digunakan untuk karakterisasi kedelapan isolat secara komprehensif. Berdasarkan analisis sekuen gen 16S rDNA, data menunjukkan bahwa isolat JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, dan JT 31 memiliki kekerabatan terdekat dengan Vibrio natriegens, V. olivaceus, V. damsella ATCC33, V. fortis, V. alginolyticus, V. harveyi, V. parahaemolyticus and V. carcharieae. Dari hasil penelitian ini dapat disimpulkan bahwa rep-PCR dapat digunakan untuk pendekatan molekuler secara efisien pada bakteri vibrio yang berasosiasi dengan kerapu sakit.Kata kunci: rep-PCR, Vibrio, Kerapu, Karimunjawa Moribound grouper fish was taken from the cages located in Karimunjawa waters. The research aim was to apply Repetitive Sequence-Based Polychain Reaction (REP-PCR) to group the vibriosis on groupers from Karimunjawa waters. Thirty two isolates of Vibrio were isolated from external wound and kidney of groupers. Based on the rep-PCR results found eight groups of vibrio bacteria associated with moribund groupers fish. Eight isolates, i.e. isolate of JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, and JT 31 were continued to characterize using the molecular techniques of 16S rDNA. Based on the results of sequen analysis, data showed that isolate of JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, and JT 31 was closely related to Vibrio natriegens, V. olivaceus, V. damsella ATCC33, V. fortis, V. alginolyticus, V. harveyi, V. parahaemolyticus and V. carcharieae, respectively . The present research concluded that rep-PCR was able to conduct biomoleculer approach efficiently for vibrios bacteria in moribund groupers fish. Key words: rep-PCR, Vibrio, groupers, Karimunjawa

Causitive Agent Vibriosis dari Ikan Kerapu Bebek (Cromileptis altivelis) Bermulut Merah : 1. Patogenitas pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Sarjito Sarjito; Ocky Karna Radjasa; Sahala Hutabarat; Slamet B Prayitno
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 3 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The research aims were to find out the causative agent vibriosis of Cromileptis altivelis having clinical symptom red mouth and its pathogenecity to Epinephelus fuscoguttatus. Seven isolats Vibrio were isolated from wound and kidney of C. Altivelis. The result of Koch postulate indicated that five vibrios as a causative agent of vibriosis, consisted of three vibrios (isolat JT 07, JT 10, JT 20) and two vibrios (isolat JT 4, JT 29) caused mortality of 100% and 40% on E. fuscogutatus respectively. Three isolat vibrios ( JT 7, JT 10, JT 20 ) with higher pathogenicity were continued to futher investigation. Mean time to death of V. fuscus (JT 07), V. alginolyticus and V. anguillarum, to E. fuscogutatus on concentration of 108 CFU/mL were 83,33% (11,25 hours); 79,16%(15,63 hours); dan 50% (20,5 hours) respectively; whereas on concentration of 109 CFU/mL were 95,83% (10,8 hours); 87,5%(15,28 hours); dan 62,5% (19,6 hours) respectively. Lethal Concentration Median (LC50) of V. Fuscus, V. alginolyticus, V. anguillarum were 3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL. All isolates on concentration of 106 dan 107 CFU/mL did not cause 50% tested fish mortality Key words : Causative agent, Vibriosis, E. Fuscogutatus, V. parahaemolyticus, Pathogenicity Penelitian ini bertujuan untuk mengkaji causative agent vibriosis pada ikan Kerapu Bebek (Cromileptis altivelis) dengan gejala klinis mulut merah serta patogenisitasnya terhadap ikan Kerapu Macan (Epinephelus fuscoguttatus). Tujuh isolat Vibrio berhasil diisolasi dari bagian luka dan ginjal Kerapu Bebek Mulut Merah. Hasil uji postulat koch memperlihatkan lima isolat dimana isolat JT 07, JT 10, JT 20 dapat mengakibatkan kematian 100%, sedangkan isolat JT 04 dan JT 29 menyebabkan kematian 40%. Pada tiga isolat (Vibrio JT 07, JT 10 dan JT 20) yang memperlihatkan patogenitas yang lebih tinggi dilakukan uji lanjutan. Hasil karakterisasi melalui uji morfologi dan biokimia diperoleh bahwa isolat JT 07 memiliki kemiripan 96,15 % dengan Vibrio fuscus; JT 10 memiliki kemiripan 100% dengan Vibrio anguillarum dan JT 20 memiliki kemiripan 100% dengan Vibrio alginolyticus. Patogenisitas ketiga isolat vibrio tersebut secara berurutan adalah V. fuscus, V. alginolyticus, V. anguillarum, dimana diperoleh bahwa persentase kematian dan rerata waktu kematian (Mean Time to Death, MTD) pada penyuntikan intraperitoneal V. fuscus (JT 07), V. alginolyticus (JT 20) dan V. anguillarum (JT 10) dengan konsentrasi 108 CFU/mL adalah berturut-turut 83,33% (11,25 jam); 79,16% (15,63 jam); dan 50% (20,5 jam); sedangkan untuk konsentrasi 109 CFU/mL secara berurutan adalah 95,83% (10,8 jam); 87,5% (15,28 jam); dan 62,5% (19,6 jam). Lethal Concentration Median (LC50) V. fuscus, V. alginolyticus, V. anguillarum secara berurutan adalah sebesar 3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL. Sedang pada konsentrasi 106 dan 107 CFU/mL semua isolat tidak menimbulkan kematian pada ikan uji. Hasil ini menunjukkan bahwa tiga causative agent tersebut bersifat patogen pada ikan kerapu. Kata kunci: Causative agent, Vibriosis, E. fuscogutatus V. parahaemolyticus, Patogenisitas

Pengaruh Temperatur dan Photoperiod Terhadap Kematangan Gonad Kepiting Bakau (Scylla serrata) Ali Djunaedi; Adi Santoso; W. Widiatmoko; Sarjito Sarjito
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 2 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian dilakukan untuk mengetahui pengaruh photoperiod dan temperatur terhadap pematangan gonad kepiting bakau. Penelitian dilaksanakan di Hatchery Marine Center IImu Kelautan Jepara, dari bulan September 1999 sampai dengan bulan Desember 1999. Metoda penelitan menggunakan rancangan acak lengkap (RAL) faktorial faktor pertama adalah perlakuan photoperiod dengan 2 taraf pelakuan. yaitu shortday dan long-day. Sedangkan faktor kedua adalah perlakuan temperatur yang juga mempunyai 2 taraf perlakuan yaitu 29 oC dan 31 oC. Hasil penelitian menunjukksn bahwa perlakuan dengan kombinasi photoperiod dan temperatur berpengaruh terhadap perkembangan gonad kepiting bakau. Urutan besarnya nilai lndek kematangan gonad (IKG) adalah 25,41 (A 1B I); 22,06 (A 1B2):20,77 (A2B I) dan 19,88 (A2B2). Hasil analisis regresi nilai simpangan (b) dari persamaan garis menunjukkan nilai IKG terbaik dicapai pada perlakuan short-day dan temperatur 29oC (A 1B1). Sedangkan terendah pada perlakuan long-day dan temperatur 31°C (A2B2).Kata kunci: reproduksi, photoperiod, temperatur; indek kematangan gonad The research is to investigate the effect of photoperiod and temperature on the gonad development (GSI) of the mud crab. This research was conducted at the marine centre hatchery Jepara from September to December 1999. The method used was completely factorial randomized design. The first factor was photoperiod i.e. short-day and long-day. The second factor was i.e temperetur 29 oC and 31 oC. The result showed that photoperiod and temperatur in combination influenced gonad development of the mud crab. The degree of the gonado somatic index (GSI) was 25,41 (A 1B1); 22,06 (A 1B2); 20,77 (A2B1) and 19.88 (A2B2). The result of regression analysis showed that the highest and the lowest gonado somatic indices occured on the combination between short-day and 29 oC and long-day temperatur 3 1oC respectively. Keywords: reproduction, photoperiod, temperatur; and gonado somatic index (GSI)

Fenomena Pertumbuhan Compensatory dan Kualitas Ikan Nila Merah (Oreochromis sp.) pada Kondisi Laut Adi Santoso; Sarjito Sarjito; Ali Djunaedi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 2 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian skala laboratorium untuk mengevaluasi fenomena pertumbuhan compensatory dan kualitas produk (body composition) dari nila merah (Oreochromis sp.) yang dipelihara di air laut dilakukan pada awal Juni sampai awal Agustus 2003 di Hatchery Kampus Kelautan Teluk Awur Jepara FPIK UNDIP. Benih ikan (37,74gr + SD 1,16gr) sebelumnya diaklimatisasikan pada kondisi laut dan dipelihara dalam bak-bak percobaan dengan kepadatan 5 ekor/m3. Perlakuan pemuasaan dengan 3 kali ulangan selama 4 minggu percobaan, yaitu: ikan diberi pakan setiap hari (A/kontrol); diberi pakan selama 6 hari diikuti pemuasaan 1 hari (B); diberi pakan selama 5 hari diikuti pemuasaan 2 hari (C); dan, diberi pakan selama 4 hari diikuti pemuasaan 3 hari (D). Pakan berbentuk pellet tenggelam (PT CP Prima) dengan kandungan protein 24-26%, lemak 3-5%, serat kasar 4-6%, abu 5-8% dan air 11-13%, diberikan 2 kali sehari sebanyak 5% dari biomassa. Pengamatan pertumbuhan dilakukan seminggu sekali. Analisa body composition dilakukan untuk mengukur kandungan protein, lemak, karbohidrat dan air (%). Hasil penelitian memperlihatkan bahwa tidak terjadi perbedaan dalam pertumbuhan selama percobaan; hal ini menunjukkan terjadi fenomena pertumbuhan compensatory. Tingkat pertumbuhanpada masing-masing perlakuan yaitu 7,42 gr/minggu (A); 7,18 gr/minggu (B); 3,44 gr/minggu (C); dan, 5,34 gr/minggu (D). Analisis body composition tidak menunjukkan adanya perbedaan dalam kandungan protein, lemak, karbohidrat, maupun air. Kandungan protein normal (>18%). Kandungan lemak rendah, 1,50-1,80%, diikuti kandungan air yang tinggi (>70%), yang menyebabkan tekstur daging lebih lunak. Kandungan karbohidrat 2,7-4,8%.Kata kunci: nila merah, pertumbuhan compensatory, kuantitas, kualitasThe study to evaluate both compensatory growth phenomenon occurred and the fish quality (body composition) were done under the laboratory conditions from beginning June to beginning August 2003 at the hatchery of Marine Science Teluk Awur-Jepara Campus, Diponegoro University. Red tilapias of mean weight of 37.74g+SD 1.16 g were acclimated in seawater conditions. The fish were cultured in the tank with a density of fivefish/m3 The treatments were feeding daily (A/control); fish fed 6 days–a day unfed (B); fish fed 5 days-2 days unfed; and fish fed 4 days-3 days unfed (D). The food was slowly sinking type (CP Prima) containing protein24-26%, oil 3-5%, fibre 4-6%, ash 5-8%, and water 11-13%. Feeding frequency was twice a day with 5% of the biomass. Growth was measured weekly. For fish quality analysis (body composition), it was done tomeasure the contents of protein, fat, carbohydrate, and water (%). The result showed that there was no significant difference of the growth among the fish (ANOVA); and, in turn, it suggested that the compensatorygrowth was occurred. The growth rates were 7.42 g/week (A), 7.18 g/week (B), 3.44 g/week(C), and 5.34 g/week (D). There was no difference for the contents of protein, fat, carbohydrate, and water. In general, theprotein content was above 18%.The low fat contents (1.50-1.80%) was followed by the high water contents (>70%), so that it made the flesh texture being soft. The carbohydrate contents were 2.74 to 4.8%.Key words: red tilapia, compensatory growth, quantity, quality

Co-Authors - Aminah - Desrina, - - Istikhanah - Susanti, - - Triyaningsih A. Harjuno Condro Haditomo A. Santoso Adhi Kurniawan Adi Santoso Afifah, Roidah Nur Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agus Dwi Anggono Agus Pranoto Syah, Agus Pranoto Agus Yulianto Aji, Nisa Pamesty Rahma Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Alfia Magfirona Alfiyani, Lina Ali Djunaedi Amalia, Ayu Rizki Amanda Mega Putri, Amanda Mega ANGELA MARIANA LUSIASTUTI Anggoro, Agung Doni Anggun Putriani Situmorang, Anggun Putriani Aninditia Sabdaningsih Anisa Dwiaryani Latifah Annisa Oktafianti Nurlatifah, Annisa Oktafianti Aprilia Dwi Indriani Arifin Arifin Arum Almuaromah, Dita Asep Akmal Aonullah Astri Pujiati Aulia Resty Wijayanti Aulia, Annisa Syahida Ayi Santika Ayu Wulandari Bosma, Roel Bosma, Roel H. Briliani Ayu Wardani Budi Setiawan Buyung Junaidin, Buyung Caesa, Genio Chairulina Pitrianingsih CHONDRORESMI BANOR FAWWAZ Chyntia Arindita Dani Indrarini David Panca Wijaya, David Panca Desrina Desrina Dewi Nurhayati Dhani Mutiari Dian Ratna Sari Dian Wijayanto Diana Chilmawati Diana Chilmawati Diana Rachmawati Dicky Harwanto Ditha Cahyaningrum Ditha Febriana Dewanti Nineung Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Ervia Yudiati Fajar Basuki Famelia Meta Putri Fandy Malik Muhammad, Fandy Malik Fatian, Adella Spextania Ferdian Bagus Feriandika Fifiana Zulaekah Fitriadi, Ren Gina Saptiani Haeruddin Haeruddin Hasna, Salma Khoironnida Hasyim Asyari Ika Puspitasari Indah Febry Hastari Intan Eska Amalia Syahida Istiyanto Samidjan Jery, Jery Johannes Hutabarat Jokosisworo Jokosisworo Kewa, Kristofora Karolina Khuzaimah, Ima Siti Kurniawan Kurniawan Laksono Trisnantoro Lestari Lakhsmi Widowati Lilik Maslukah Lilik Setiyaningsih Linuwih Aluh Prastiti Lukman Anugrah Agung Lukman Lukman Marwenni Siregar, Marwenni Milza Apriliani, Milza Mita Umiliana, Mita Monica Nanda Muchtar muchtar Muhammad Burhan Mukhlisin, Latutik Nailil Muna Nida Qolbi Salma Rochani Noor Alis Setiyadi Nur Aklis Nur Annisa Nuri Nia Yanti, Nuri Nia Nurul Hidayati Ocky Karna Radjasa Panji Yusroni Anwar Prabowo, Anggit Bayu Pramudita Apriliyanti Prayitno, S. Budi Pungki Nanda Pratama Purwanto Purwanto Pusaka, Semerdanta R. Dewi Dharina Nurjannah Rahman, Nuril Endi Rahmawati, Amelia Rahmi Gusti Darma Raynol Simorangkir Rensiga Rintan Bunga Sari Restiana Ariyati Restiana Wisnu Ariyati Rini, Endah Setyo Ristiawan Agung Nugroho Rohita Sari Rosa Amalia Rosalina Safitri Rusydina Qamarul Salikin S. Budi Prayitno Sahala Hutabarat Sarastiti, Siwi Schrama, Johan Sekar Ayu Chairunnisa Seto Windarto Setyo Putro Rahmanto Setyowati, Suryaning Siti Nurjanah Siti Ziyadaturrohmah Siwi Hartanti Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Soedibya, Petrus Hary Tjahja Sri Hastuti Sri Hastuti Sri Nurchayati Sri Nuryati Sri Rejeki Subagiyo Subagiyo Subandiyono Subandiyono Subroto Subroto Sulisyaningrum Sulisyaningrum Sumini Sumini, Sumini Suminto , Suminto - Suminto Suminto Suminto Suminto Suminto Suminto Suminto Suminto Suradi Wijaya Saputra Tita Elfitasari Titik Susilowati Tri Mulyadi Trienes, Yoni W. Widiatmoko Wiji Utami, Wiji Wijianto Wijianto Wisnu Widyantoro Wiyadi Yelliana Fatmawati Suwarno Yohanes Kristiawan Artanto

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