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All Journal Maspari Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Rotasi Jurnal Pasir Laut Journal of Aquaculture Management and Technology Media Mesin: Majalah Teknik Mesin Jurnal Kelautan Tropis Omni-Akuatika Turbo : Jurnal Program Studi Teknik Mesin Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Riset Akuakultur Scripta Biologica Journal of Aquaculture and Fish Health PENA AKUATIKA : JURNAL ILMIAH PERIKANAN DAN KELAUTAN Jurnal Media Akuakultur Indonesia Abdi Geomedisains Journal Of Human And Education (JAHE) Warta LPM Journal of Community Services and Engagement: Voice of Community (VOC) Majalah Teknik Mesin
Sarjito -
Departemen Akuakultur, Universitas Diponegoro Jl. Prof. Soedharto S.H., Semarang 50275, Indonesia, Telp: +62821 5350 5993, Fax: 0247474698
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Identifikasi Bakteri Agensia Penyebab Motile Aeromonas Pada Ikan Lele Dumbo (Clarias gariepinus) yang Berasal dari Kecamatan Rowosari, Kabupaten Kendal Apriliyanti, Pramudita; -, Sarjito; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Bacterial disease that often affects catfish was Aeromoniasis caused by Aeromonas sp. This disease was considered as an exotic disease since it reslted in mas mortality over 60% within 7 days. The purpose of this study was to determine the clinical signs of fish sample that affected by bacteria. To identifiy the causative agent of bacterial disesase morphological and biochemically, and then to observe the pathogenicity of the selected motile Aeromonas by postulat koch test. The research method used was a combination between exploration and case study. Purposive random sampling was used to collect moribund catfish. Observation of bacterial clinical signs was carried out to 10 moribund catfish that purposively rondomized. Isolation of bacteria from infected skin and kidney were conducted on aeromonas special medium GSP. Purification was done by recultured the selected colony 3 - 5 times on GSP medium. Three (3) selected isolate for Postulate Koch was cultured in Zobelt medium, and then intramusculary injected to 10 tested catfish of 10 - 15 cm, with concentration 108 clinical response and mortality rates were observed every 6 hours for 96 hours. Finally three tested bacteria were identified morphological and biochemically. The results showed clinical signs that clinical signs of moribund catfish related to Aeromonas infection. This could be seen from the presence of haemorrhagic and pethicia of the body skin such as at abdomen, dorsal and tail fins, exopthlamic and gill necrosis. From the 10 catfishes it could be isolates 16 bacterial colonies. Three selceted isolates namely LPK.GSP3.pk, LPK.GSP4.p and LPK.GSP6.k showed their pathogenicity on the postulat koch test. LPK.GSP6.k demonstrated their pathogen caused 35% mortality and 39% moribund, whilst LPK.GSP3.pk and LPK.GSP4.p equally caused 20% mortality and 47% moribund. Morphoogical and biochemical karacterization of the three isolates above indicated that three isolates closely related to Aeromonas caviae, Aeromonas hydrophila and Aeromonas salmonicida. This was might be the reason why these three bacterial isolates quite virulence.
PENGARUH PERENDAMAN EKSTRAK DAUN SIRIH (Piper betle) DENGAN KONSENTRASI YANG BERBEDA TERHADAP GEJALA KLINIS, KELULUSHIDUPAN, HISTOLOGI DAN PERTUMBUHAN UDANG VANAME (Litopenaeus vannamei) YANG DIINFEKSI Vibrio harveyi Annisa, Nur; Sarjito, -; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Volume 4, Nomor 3, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Bakteri Vibrio harveyi merupakan salah satu agensia penyebab vibriosis yang dapat menyebabkan kematian pada udang, sehingga menimbulkan kerugian secara ekonomi. Tujuan dari penelitian ini adalah mengetahui pengaruh konsentrasi perendaman ekstrak daun sirih (Piper betle) berbeda terhadap gejala klinis, kelulushidupan, histologi dan pertumbuhan udang Vaname (L. vannamei) yang diinfeksi V. harveyidan mengetahui konsentrasi terbaik dari perendaman ekstrak daun sirih (Piper betle).Udang uji yang digunakan berjumlah 120 ekor dengan ukuran ±7 cm. Penginfeksian V. harveyi sebanyak 0,1 mL dengan konsentrasi 106 CFU/ml pada bagian intramuskular.Pada penelitian ini digunakan konsentrasi ekstrak daun sirih dengan konsentrasi perlakuan 0 ppm, 500 ppm, 800 ppm dan 1100 ppm. Gejala klinis yang muncul adanya warna memerah pada bagian ekor dan telson serta seluruh tubuh, perubahan warna kehitaman pada hepatopankreas serta udang terlihat pasif.  Perendaman (dipping) dilakukan selama 10 menit. Hasil penelitian diperoleh bahwa perendaman ekstrak daun sirih memberikan pengaruh nyata terhadap kelulushidupan dan histopatologi hepatopankreas udang vaname akan tetapi tidak berpengaruh nyata terhadap pertumbuhan udang vaname. Hasil ini juga diperoleh bahwa penggunaan konsentrasi1100 ppmmerupakan konsentrasiekstrak daun sirih terbaik untuk mengobati udang vaname yang terinfeksi V. harveyi. Vibrio harveyi isone of vibriosis agents that couldmass mortality in farmed shrimp, therefore result in significant losses in shrimp farming. The purpose of this researchis to find out the effect of various immersion concentration of betel leaves extract (Piper betle) toward clinical symptoms, survival rate, histology and growth of vannamei (L. vannamei) thatinfectedby V. harveyi and to determine the best concentration of immersion betel leaves extract (Piper betle).Infection was carried out by intramuscular infection of 0,1 ml of 106 CFU/ml V. harveyi. When clinical sign was shown redness on the tail and telson up the whole body flushed, discoloration and shrimp hepatopancreas blackened shrimp ponds passive then soaked with betel extract for 10 minutesThe result of the betel leavesextract immersion that give a significant effect on survival and histopathological hepatopancreas vaname but, it did not significantly affect the growth of shrimp vannamei. The result of this research which are the using concentration 1100 ppm is the best concentration to treat infected white shrimp V. harveyi.
AGENSIA PENYEBAB VIBRIOSIS IKAN LELE DUMBO (Clarias gariepinus) PADA KOLAM BEKAS TAMBAK UDANG Indrarini, Dani; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Tingginya permintaan pasar terhadap ikan lele menyebabkan pembudidaya bekerja keras untuk meningkatkan hasil produksi melalui upaya budidaya intensif dalam pemanfaatan lahan bekas tambak. Seiring dengan adanya pemanfaatan lahan bekas tambak yang memiliki kandungan salinitas rendah, maka dimungkinkan untuk terdeteksinya bakteri genus Vibrio. Vibriosis merupakan penyakit bakterial yang sangat merugikan usaha budidaya ikan karena dalam waktu yang sangat singkat dapat menimbulkan tingkat kematian yang tinggi. Metode yang digunakan adalah metode eksploratif. Ikan sampel diambil dari Desa Bulumanis Kabupaten Pati dan Desa Desa Wonosari Kabupaten Demak sebanyak 10 ekor yang diduga terserang penyakit bakteri. Isolasi bakteri menggunakan media TCBS. Organ yang diisolasi yaitu luka – luka pada permukaan tubuh, hati, dan ginjal ikan lele. Hasil isolasi diperoleh 23 isolat lalu diseleksi berdasarkan morfologi koloni hingga diperoleh 5 isolat (LPL14, LDL8, LPG10, LPL4, dan LDH1) untuk uji postulat koch. Hasil penelitian menunjukkan bahwa gejala klinis ikan lele yang terserang Vibriosis adalah luka kemerahan/borok (ulcer) pada permukaan tubuh, hemoragi (luka kemerahan), perut berisi cairan kuning dan sirip gripis yang disertai luka kemerahan. Identifikasi bakteri dilanjutkan dengan uji biokimia. Agensia penyebab Vibriosis ikan lele pada kolam bersalinitas rendah adalah bakteri genus Vibrio  (LPL 14, LDL 8, dan LPG 10), Vibrio vulnificus (LDH 1), dan Vibrio harveyi (LPL4). Pengamatan histopatologi diperoleh bahwa terjadi kerusakan pada organ hati berupa kelainan nekrosis, degenerasi vakuola, melanomakrofag, dan kongesti. High market demand of catfish causes an increase of the farmer effort to increase the production by extensification using unproductive brackish pond area. In a row of using a brackish pond area which has a low salinity, consequently Vibriosis are detected in the pond culture. Vibriosis is a bacterial diseases that can causes loss in aquaculture at a short time can lead a high mortality rate. Research method used explorative method. 10 samples of fish were taken from Bulumanis village, Pati regency and Wonosari village, Demak regencies which were potentially infected by Vibrio. Isolation of bacteria were done in TCBS medium. Bacterial isolates were collected from fish lesion on the body surface, liver, and kidney of catfish. Isolation were able to gained 23 isolates and then 5 isolates (LPL14, LDL8, LPG10, LPL4, and LDH1) were selected based on colony morphology to do postulates koch’s test. The results of this research showed that the clinical signs of catfish infected by Vibrio were redness lesions/ulcer on the body surface, hemorrhagic, fluid inside stomach, and fin eroded with redness wound. Bacterial identification through biochemical test revealed that the causative agent of catfish disease at brackish pond area were bacteria of the genus Vibrio (LPL 14, LDL 8, and LPG 10), Vibrio vulnificus (LDH 1), and Vibrio harveyi (LPL4). Observation of histopathology found necrosis, vacuolar degeneration, melanomacrofage, and congestion in the liver.
PERENDAMAN BERBAGAI DOSIS EKSTRAK DAUN BAKAU (Rhizophora apiculata) UNTUK PENGOBATAN KEPITING BAKAU (Scylla serrata) YANG DIINFEKSI BAKTERI Vibrio harveyi Putri, Amanda Mega; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Budidaya kepiting bakau (Scylla serrata) sering terkendala karena serangan penyakit bakterial Vibrio harveyi. Penggunaan antibiotik dalam jangka panjang dapat menimbulkan resistensi dan mencemari lingkungan. Penggunaan bahan alami untuk pengobatan infeksi bakteri sedang digalakkan, salah satunya adalah ekstrak daun bakau (Rhizophora apiculata). Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak daun bakau terhadap kelulushidupan, laju pertumbuhan spesifik, dan histopatologi hepatopankreas kepiting serta mengetahui dosis terbaik untuk mengobati kepiting yang diinfeksi bakteri V. harveyi. Kepiting bakau yang digunakan berjumlah 48 ekor dengan berat tubuh antara 40.11±2.53 gram, yang kemudian disuntik bakteri V. harveyi dengan kepadatan 106 CFU/ml secara intramuskular. Perendaman ekstrak daun bakau dilakukan 60 – 90 menit pasca penyuntikan setelah muncul gejala klinis seperti merenggangnya kaki renang dan kaki jalan, karapas menghitam, terdapat bintik putih, dan munculnya bercak merah. Perendaman ekstrak daun bakau berpengaruh sangat nyata (P<0.05) dan P(<0.01) terhadap kelulushidupan kepiting yang diinfeksi bakteri V. harveyi. Nilai rerata kelulushidupan tertinggi hingga terendah berturut-turut yaitu 100% (perlakuan D), 66.67% (perlakuan C), 58.33% (perlakuan B), dan 41.67% (perlakuan A). Sebaliknya, perendaman ekstrak daun bakau tidak memberikan pengaruh nyata (P>0.05) dan P(>0.01) terhadap pertumbuhan kepiting bakau. Hasil pengamatan histopatologi diperoleh adanya kerusakan berupa nekrosis dan vakuolisasi pada organ hepatopankreas. Pada penelitian ini, dosis 900 ppm merupakan dosis terbaik dalam mengobati kepiting bakau yang diinfeksi V. harveyi yang mampu memberikan efek penyembuhan terhadap kepiting bakau. Mud crab (Scylla serrata) culture often found obstacle due to bacterial infection Vibrio harveyi. Long-term use of antibiotics cause bacterial resistante and contaminated the environment. Natural ingredients has been for treatment of diseased crab, one of which was mangrove leaf extract (Rhizophora apiculata). This research was aimed to observe the effect of immersion mangrove leaf extract to the survival rate, specific growth rate, and histopathological picture of hepatopancreas mud crab and to know the best dose to treat mud crab that was infected by bacteria V. harveyi. Mud crab used in this research was 48 animals  with body weight between 40.11±2.53 grams, then injected by bacteria V. harveyi with a density of 106 CFU/ml intramuscularly. Dipping in mangrove leaf extract was conducted for 60 – 90 minutes after injection with the appearance of clinical signs such as wide apart swimming and walking legs, blackened carapace, white spots, and red spots. Dipping of mangrove leaf extract significantly effect (P<0.05) and P(<0.01) on the survival of mud crab that was infected by bacteria V. harveyi. The average value of highest to the lowest survival 100% (treatment D), 66.67% (treatment C), 58.33% (treatment B), and 41.67% (treatment A) respectively. The other way, the submersion with mangrove leaf extract showed not giving significant different (P>0.05) and P(>0.01) on growth of mud crab. Histopathological observations obtained were necrosis and vakuolisation in hepatopancreas organ. In this research, dose of 900 ppm give the best treatment to mud crab (S. serrata) were infected V. harveyi. Therefore, immersion mangrove (R. apiculata) leaf extract was able to provides a healing effect on mud crab infected bacteria V. harveyi.
PENGGUNAAN EKSTRAK JAHE MERAH (Zingiber officinale var. Rubrum) SEBAGAI ALTERNATIF PENGOBATAN IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI BAKTERI Aeromonas hydrophila Indriani, Aprilia Dwi; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ikan nila (Oreochromis niloticus) merupakan jenis ikan air tawar yang mudah dipelihara. Intensifikasi budidaya dapat menyebabkan serangan penyakit bakteri seperti Aeromonas hydrophila. Hal ini dapat menyebabkan kerugian ekonomi dalam kegiatan budidaya. Upaya yang dilakukan untuk mengobati serangan bakteri A. hydrophila salah satunya menggunakan bahan alami, ekstrak jahe merah (Zingiber officinale var. Rubrum). Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak jahe merah untuk mengobati ikan nila yang diinfeksi  A. hydrophila. Ikan nila yang digunakan sebanyak 120 ekor dengan ukuran 7 – 9 cm kemudian disuntik bakteri A. hydrophila dengan kepadatan 108 CFU/ml secara intramuskular. Pelarut yang digunakan dalam ekstraksi jahe merah adalah ethanol 96%. Pengobatan ini menggunakan metode short bathing selama 8 menit dan dilakukan sebanyak 2 kali selama penelitian, yaitu hari pertama dan hari ke 8 setelah penyuntikan. Penggunaan ektrak jahe merah berpengaruh nyata (P<0,05) untuk mengobati ikan nila yang diinfeksi A. hydrophila dan ekstrak ini mampu menyembuhkan luka (ulcer) pada ikan nila yang diinfeksi A. hydrophila dalam 14 hari. Nilai rata-rata kelulushisupan tertinggi hingga terendah berturut-turut yaitu 57,78% (perlakuan C), 46,71% (perlakuan B), 40,78% (perlakuan D) dan 8,85% (perlakuan A). Tilapia (O. niloticus)  is one of the freshwater species which is easy to be cultured. Intensification of Tilapia causes bacterial diseases, such as A. hydrophila. This case resulted in economic disadvantage in aquaculture. One of the effort used to eliminate A. hydrophila infection was the usage of natural ingredient, red ginger (Zingiber officinale var. Rubrum) extract. The aims of this research was to determine the effect of red ginger extract for healing Tilapia infected by A. hydrophila. The Tilapia used were 120 fish, around 7 – 9 cm in size and were injected by bacteria A. hydrophila with density 108CFU / mL intramuscularly. Red ginger extract was obtained by dipping in ethanol 96%. This treatment used short bathing method for 8 minutes and it was done as much as 2 times, at the 1st and 8th days post-injection. The utilization of red ginger extract showed a significant effect (P<0.05) for healing Tilapia infected by A. hydrophila and this extract could heal ulcer on Tilapia infected by A. hydrophila in 14 days. The results showed that the average value of the highest to the lowest survival in a row were 57.78% (treatment C), 46.71% (treatment B), 40.78% (treatment D) and 8.85% (treatment A) respectively.
EFIKASI PERENDAMAN EKSTRAK SAMBILOTO (Andrographis paniculata Ness) DENGAN SALINITAS BERBEDA DAN PENGARUHNYA PADA KELULUSHIDUPAN SERTA INDEKS FAGOSITOSIS IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI Aeromonas hydrophila Darma, Rahmi Gusti; Sarjito, -; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ikan nila (O. niloticus) merupakan ikan air tawar yang dapat hidup pada air bersalinitas. Salinitas dipengaruhi oleh reaksi osmotik dalam tubuh ikan tetapi kemampuan yang dimilliki ikan nila untuk mempertahankan salinitas berbeda tidak mampu melindungi dari serangan bakteri. Upaya yang dilakukan untuk mencegah serangan bakteri A. hydrophila salah satunya menggunakan bahan alami yaitu ekstrak sambiloto (A. paniculata Ness) yang dimasukan pada media salinitas berbeda. Penelitian ini bertujuan mengetahui konsentrasi salinitas yang paling efektif untuk menyerap ekstrak sambiloto dan pengaruh ekstrak sambiloto terhadap kelulushidupan ikan nila yang diinfeksi A.hydrophila. Ikan nila yang digunakan sebanyak 120 ekor dengan ukuran 7,5±0,03 cm. Perendaman ikan dengan salinitas 0, 5, 10, dan 15 ppt. Dosis ekstrak yang digunakan adalah 100 ppm dalam 5 L air dengan 3 kali ulangan dan direndam selama 5 jam. Setelah itu ikan disuntik bakteri A. hydrophila dengan kepadatan 108 CFU/ml. Nilai rata-rata kelulushidupan tertinggi hingga terendah berturut-turut yaitu 81,14% (perlakuan C), 78,93% (perlakuan D), 72,29% (perlakuan B) dan 54,70% (perlakuan A). Perendaman ikan dengan salinitas berbeda tidak berpengaruh nyata terhadap kelulushidupan ikan nila yang diinfeksi A. hydrophila. Perendaman ikan pada salinitas 10 ppt yang ditambahkan ekstrak sambiltoto merupakan perendaman terbaik yang memberi pengaruh pada indeks fagositosis. Diketahui nilai tertinggi indeks fagositosis perlakuan C (70,33%).  Tilapia (O. niloticus) is one of fish fresh water can live in the salinity. Salinity affected by osmotic reaction in fish body but the ability in tilapia for maintain by different salinity not able to protect bacterial desease. Efforts are being made to prevent bacterial attack one A. hydrophila using natural ingredients that extracted from sambiloto (A. paniculata Ness) and put into in different salinity media. The aim of this study to determine the effective concentration of salinity to absorb the effect of the extract of sambiloto and effect of sambiloto extract on survival of tilapia which has infected by A.hydrophila. 120 Tilapia this research used with lenght of 7.5 ± 0.03 cm. The treatment used with a salinity of 0, 5, 10, and 15 ppt . With extract dose 100 ppm in 5 L of water with 3 replications and deeping for 5 hours . After that, the  fishes were injected with bacteria A. Hydrophila in density of 108 CFU / ml . The average value of the highest survival rate to the lowest row is 81.14 % ( treatment C ), 78.93 % ( treatment D ), 72.29 %  ( treatment B ) and 54.70 % ( treatment A ) . The deeping treatment in different salinity did not significantly affect survival of tilapia which infected by A. hydrophila . Soaking the fish at 10 ppt salinity with sambiltoto extract gives the best immersion effect on phagocytosis index. It is discovered that the highest value of phagocytosis index treatment C ( 70.33 % ) .
Performa Profil Darah Lele Dumbo (Clarias gariepinus) Yang Terserang Penyakit Kuning Setelah Pemeliharaan Dengan Penambahan Vitamin C Pada Pakan Hartanti, Siwi; Hastuti, Sri; -, Sarjito
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Abstrak Vitamin C yang ditambahkan dalam pakan diharapkan dapat mengurangi stress dan mempercepat proses penyembuhan luka pada ikan. Tujuan dari penelitian ini adalah untuk mengetahui performa profil darah pada lele dumbo (Clarias gariepinus) yang terserang penyakit kuning setelah penambahan vitamin C pada pakan dan mengetahui nilai profil darah setelah penambahan vitamin C pada pakan. Hasil penelitian menunjukkan bahwa pemberian vitamin C tidak berpengaruh nyata (P>0,05) terhadap nilai profil darah ikan lele dumbo (C. gariepinus). Nilai eritrosit tertinggi pada perlakuan A sebesar 2,02±0,26 x106 sel/ul, nilai leukosit tertinggi pada perlakuan A sebesar 102±9,86 x103 sel/ul, nilai hemoglobin tertinggi pada perlakuan A sebesar 7,9±1,03 g/dl, nilai hematokrit tertinggi pada perlakuan A sebesar 24,07±3,83 %, nilai glukosa darah tertinggi pada perlakuan D sebesar 48,67±4,04 mb/L, nilai biliriubin direk tertinggi pada perlakuan A  sebesar 0,17 mg/dl, bilirubin indirek nilai tertinggi pada perlakuan B, C dan D  sebesar 0,13 mg/dl, nilai bilirubin total tertinggi pada perlakuan C sebesar 0,3 mg/dl, nilai SGPT tertinggi ada pada perlakuan C sebesar 21,33±2,08 U/L dan pada nilai SGOT tertinggi ada pada perlakuan B sebesar 103,67±21,50 U/L. Hasil pengamatan jaringan hati ikan lele dumbo kuning menujukkan perubahan struktur hati pada semua perlakuan selama 4 minggu. ABSTRACT Vitamin C which was added in feed was expected to reduce stress and accelerate the process of healing wounds in fish. The purpose of this research is to find out the performence profile of blood dumbo catfish (Clarias gariepinus) by the yellow disease after increment vitamin C on feed and find out the profile blood value after increment vitamin C on feed. The result from this research, that vitamin C addition was not significantly affect  (P>0,05) to the profile of dumbo cathfish (C. gariepinus) blood sample. The highest Erythrocytes value of treatment A was 2,02±0,26 x106 sel/ul, the highest leukocyte value of treatment A was 102±9,86 x103 sel/ul, the highest hemoglobin value of treatment A was 7,9±1,03 g/dl, the highest Hematokrit value of treatment A was 24,07±3,83 %, the highest blood glucose value of  treatment D was 48,67±4,04 mb/L, the highest direct biliriubin value of treatment A was 0,17 mg/dl, the highest indirect Bilirubin value of treatment B, C and D were 0,13 mg/dl, The highest value of total of bilirubin in treatment C was 0,3 mg/dl, the highest value og  SGPT was exist in  treatment C of 21,33±2,08 U/L, and the highest value of SGOT was exist in treatment B as much as 103,67±21,50 U/L. The results from observation of heart tissue catfishes which were affected by the  yellow disease,  indicated stucture changed  of its heart in all treatment for four weeks.  
PATOGENISITAS ISOLAT K14 YANG DIISOLASI DARI LELE DUMBO (Clarias gariepinus) YANG BERASAL DARI DEMAK Wulandari, Ayu; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Vol 3. No 2 (2014): Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Budidaya lele (Clarias gariepinus) berkembang pesat dan memiliki nilai ekonomis yang tinggi. Ikan lele dapat dibudidayakan di lahan dan sumber air tebatas serta padat penebaran tinggi.  Pertumbuhan ikan lele cepat dan pemasaran mudah. Penelitian ini bertujuan untuk mengetahui tingkat patogenisitas dan leukosit total ikan lele dumbo (C. gariepinus) yang telah diinfeksi Isolat bakteri K14 dengan kepadatan yang berbeda serta identifikasi Isolat bakteri K14 pada lele dumbo secara biomolekuler berbasis PCR 16S rDNA. Penelitian ini menggunakan metode eksperimental. Ikan uji yang digunakan adalah ikan lele dumbo sebanyak 210 ekor dengan ukuran 8.02 ± 0.6 cm Kepadatan Isolat bakteri K14 yang digunakan dalam penelitian adalah perlakuan (A) 100 CFU/mL, perlakuan (B) 104 CFU/ml, perlakuan (C) 105 CFU/ml, perlakuan (D) 106 CFU/ml, perlakuan (E) 107 CFU/ml, perlakuan (F) 108 CFU/ml, dan perlakuan (G) 109 CFU/ml. Pengamatan gejala klinis dilakukan selama 96 jam dan pengamatan leukosit total dilakukan setiap 24 jam sekali selama 6 hari. Hasil penelusuran sequen 16S rDNA mengunakan internet melalui sistem BLAST. Hasil pengamatan gejala klinis lele dumbo yang diinfeksi Isolat bakteri K14 adalah ikan berenang lemah di dasar air, berenang vertikal, nafsu makan berkurang, kulit mengelupas, haemorragic pada tubuh, kemudian timbulnya radang kemudian menjadi luka yang terbuka (ulcer). Kematian ikan tertinggi terlihat pada perlakuan F (108 CFU/mL) dan G (109 CFU/mL) yaitu 30 ekor, kemudian perlakuan E (107 CFU/mL) sebesar 24 ekor, perlakuan D (106 CFU/mL) sebesar 23 ekor, perlakuan C (105 CFU/mL) sebesar 22 ekor, dan kematian ikan paling sedikit dialami pada perlakuan B (104 CFU/mL) sebesar 2 ekor. Nilai LD50 dalam uji patogenisitas menunjukkan bahwa konsentrasi bakteri yang dapat mematikan 50% populasi ikan lele dumbo dalam waktu 96 jam adalah 4.977x105 CFU/ml. Jumlah leukosit total tertinggi setelah 24 jam penyuntikan Isolat bakteri K14 pada perlakuan G adalah 3.41x104 sel/mm3 dan terendah pada perlakuan A sebanyak 1.57x104 sel/mm3. Hasil penelusuran berdasarkan 16S rDNA menggunakan sistem BLAST diperoleh bahwa homogenitas sebesar 96% terhadap Aeromonas sp. Catfish farming have high value and high demand because a catfish can growt in limited water resources with high density. Catfish growth fast and easy to sell them. The purpose of this research was to determine the level of pathogenicity of K14 isolate to catfish (C. gariepinus that have inframoscularly nipelia misl K14 isolateat different densities and then biomolecularly identified based on PCR 16S Rdna. This research used experimental method. The fish sample was used catfish with 8.02 ± 0.6 cm leght. The density of bacteria used in this research were (A) PBS, (B) 104 CFU/mL, (C) 105 CFU/mL, (D) 106 CFU/mL, (E) 107 CFU/mL, (F) 108 CFU/mL, (G) 109 CFU/mL. The observation of clinical signs carried out for 96 hours and total leukocytes were observed every 24 hours for 6 days. 16S rDNA  sequences of K14 isolate was casuistry the BLAST system. The clinical sign of catfish that infected by K14 isolates obtained that fish showed swimming weakly, found till vertically, poor appetite, skin peeling, haemoragic then followed by the inflammation and ulcer. The highest fish mortality was seen in the treatments F (108 CFU/mL) and G(109 CFU/mL) by 30 animals, E (107 CFU/mL) treatments 24 animal, D treatment  106 CFU/mL was valued 23 animals, C treatment 105 CFU/mL was 22 animals and B treatment 104 CFU/mL was 2 animals respectively. LD50 value of K14 demonstrated that the concentration of 105 CFU/mL has able to kill 50% of treated catfish within 96 hours. The highest total leukocytes after 24 hours injection of K14 isolate was found in the G treatment valued 3.41 x 104 cells/mm3 and the lowest found in A treatment at 1.57 x 104 cells/mm3. Reidentification of K 14 isolate using moleculer approach 16S rDNA then presented the sequence againt existing gen bank by BLAST system indicated that genetically the K14 isolate closes 96% to Aeromonas sp.
PENGARUH KONSENTRASI KONSORSIUM BAKTERI K1, K2 DAN K3 TERHADAP STATUS KESEHATAN RUMPUT LAUT (Eucheuma cottonii) Siregar, Marwenni; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Eucheuma cottonii berperan sebagai penyumbang utama produksi perikanan. Faktor penghambat produksi adalah penyakit ice-ice. Ice-ice disebabkan oleh lingkungan yang tidak sesuai, rumput laut akan stres sehingga melepaskan senyawa haloamin menimbulkan lendir dan memicu tumbuhnya bakteri. Penelitian ini bertujuan untuk mengetahui pengaruh konsorsium bakteri K1, K2 dan K3 dengan konsentrasi yang berbeda terhadap kesehatan E cottonii. Metode pada penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuannya dengan konsorsium bakteri K1,K2 dan K3 yang sama dan konsentrasi yang berbeda yaitu A (tanpa inokulasi/kontrol), B (5x106 CFU/mL), C (2.3x107 CFU/mL, dan D (2.2x108 CFU/mL). E. cottonii bobot 1 gram dan panjang 5 cm dipelihara dalam botol kaca yang diisi air laut steril 200 ml selama 9 hari. Kondisi suhu 28°C, salinitas 30 ‰, dan pH 8. Pemeliharaan menggunakan shaker kecepatan 100 rpm dan pemaparan cahaya 12 jam terang 12 jam gelap. Hasil penelitian menunjukkan konsentrasi konsorsium K1,K2 dan K3 yang berbeda berpengaruh nyata (P < 0,01) terhadap nilai pertumbuhan mutlak E. cottonii. Gejala klinis yang ditimbulkan adalah terdapat spot putih pada thallus, warna thallus memudar, cabang melepuh dan putus. Bakteri konsorsium yang menyebabkan gejala klinis tersebut adalah Corynebacterium, Vibrio hollisae dan Pseudomonas sp. Berdasarkan hasil penelitian disimpulkan bahwa konsorsium bakteri K1, K2 dan K3 dengan konsentrasi 2.2x108 CFU/mL dapat menyebabkan penurunan bobot dan gejala klinis tertinggi yang ditimbulkan. Eucheuma cottonii role as a major contributor to the production of fisheries. Factors inhibiting the production is ice-ice disease. Ice-ice caused by the environment are not incompatible, seaweed would stress that cause mucus haloamin releasing compounds and trigger the growth of bacteria. This study aims to determine the effect of bacterial consortium K1, K2 and K3 with different concentrations on the health of E. cottonii. The method in this study using a completely randomized design (CRD) with 4 treatments and 3 replications. Treatment with bacterial consortium K1, K2 and K3 are the same and different concentrations namely A (without inoculation / control), B (5x106 CFU / ml), C (2.3x107 CFU / ml, and D (2.2x108 CFU / ml ). E. cottonii weighs 1 gram and 5 cm long preserved in a glass jar filled with 200 ml of sterile sea water for 9 days. Conditions 28 ° C, salinity 30 ‰, and pH 8. Maintenance using a shaker speed of 100 rpm and the exposure light 12 hours of light to 12 hours dark. The results showed concentrations of a consortium of K1, K2 and K3 are different significant (P <0.01) to the value of absolute growth E. cottonii. The clinical symptoms are caused there are white spots on thallus, thallus color fading, branch blister and break up. Bacteria that cause clinical symptoms consortium is Corynebacterium, Vibrio hollisae and Pseudomonas sp. Based on the results of the study concluded that the bacterial consortium K1, K2 and K3 with a concentration of 2.2x108 CFU / ml can cause weight loss and highest clinical symptoms caused.
PENGARUH PENAMBAHAN SERBUK DAUN BINAHONG (Anredera cordifolia) DALAM PAKAN TERHADAP KELULUSHIDUPAN DAN HISTOPATOLOGI HEPATOPANKREAS UDANG VANAME (Litopenaeus vannamei) YANG DIINFEKSI BAKTERI Vibrio harveyi Sari, Rensiga Rintan Bunga; Sarjito, -; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 4, Nomor 1, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Udang vaname merupakan salah satu  jenis udang yang dibudidayakan di Indonesia karena memiliki nilai ekonomis yang cukup tinggi. Namun terdapat kendala yang dihadapi oleh pembudidaya udang antara lain adalah  serangan  penyakit. Penyakit yang diakibatkan oleh V. harveyi bersifat ganas karena dapat mematikan populasi larva udang yang terserang dalam waktu 1 sampai 3 hari. Pengobatan dengan menggunakan kombinasi berbagai antibiotik juga dapat menimbulkan masalah resistensi pada bakteri negatif. Salah satu tanaman yang dapat digunakan adalah Binahong (Anredera cordifolia). Penelitian ini bertujuan untuk mengkaji pengaruh serbuk daun binahong (A.cordifolia) terhadap kelulushidupan udang vaname (Litopenaeus vannamei) yang diinfeksi bakteri V. harveyi dan mengkaji dosis terbaik dari serbuk daun binahong yang paling efektif terhadap kelulushidupan udang vaname. Udang uji yang digunakan dalam penelitian berukuran  ±5 g dengan jumlah udang sebanyak 120 ekor. Metode penelitian ini adalah eksperimen dengan 4 perlakuan dan 3 ulangan yaitu perlakuan A (0 g/kg pakan), B (30 g/kg pakan), C (60 g/kg pakan) dan D (90 g/kg pakan). Pemberian pakan dilakukan selama 14 hari sebelum di uji tantang. Uji tantang dilakukan dengan menyuntikkan bakteri V. harveyi dengan dosis 104cfu/mm3 sebanyak 0,1 mL. Pengamatan dilakukan selama 10 hari pasca infeksi bakteri V. harveyi. Hasil penelitian menunjukkan bahwa gejala klinis udang pasca infeksi warna tubuh memerah, ekor dan kaki renang memerah, hepatopankreas berwarna coklat, dan terjadi melanosis pada karapas. Nilai kelulushidupan tertinggi hingga terendah berturut-turut 6,67% (perlakuan A), 56,67% (perlakuan D), 73,33% (perlakuan C), dan 90,00% (perlakuan B). Hasil penelitian nenunjukkan dosis 30 g/kg pakan merupakan dosis terbaik sebagai antibakteri pada udang vaname untuk mencegah penyakit V. harveyi. Vannamei is the one of shrimp species cultured in Indonesia caused has the highly economic value. But there was some, such as shrimps disease. The disease shrimps formed by V. harveyi is vicious, made mortality in curva population in 1 to 3 day. Utilization of  antibiotics caused resistance for bacteria. There was some effort to solve the effect of  resistance of antibiotics, such as using natural ingredient as an alternative treatment. The one of plants that would be herbal medicine are Binahong leaves (Anredera cordifolia). The aims of this research were to determine the effect of binahong leaves powder (A. cordifolia) againts survival rate of Vannamei (L. vannamei) infected by V. harveyi and to determine the best dose of binahong leaves powder toward survival rate of vannamei. Vannamei with total 120 shrimps weight everage ± 5 g. This research used experimental method with 4 treatments and 3 repetitions. They were treatment A (0 g/kg of feed), B (30 g/kg of feed), C (60 g/kg of feed) and D (90 g/kg of feed). The treatment done in 14 days before the challenge test. The challenge test done by injection of V. harveyi with dose 104 cell/mm3 as much as 0,1 mL . The result showed clinical sign of vannamei post infection, such as flushed body, flushed fin and swimming legs, brown hepatopancreas and melanosis on the carapace. The average value of the highest to the lowest survival are 6.67% (treatment A), 56.67%, (treatment D), 73.33% (treatment C), and 90.00% (treatment B). 30 g/kg of feed was the best dose as an immunostimulant in vannamei for preventing disease of V. harveyi.
Co-Authors - Aminah - Desrina, - - Istikhanah - Susanti, - - Triyaningsih A. Harjuno Condro Haditomo A. Santoso Adhi Kurniawan Adi Santoso Afifah, Roidah Nur Agatya Sara Ardiantami, Agatya Sara Agil Setya Utomo, Agil Setya Agus Dwi Anggono Agus Pranoto Syah, Agus Pranoto Agus Trianto Agus Yulianto Aji, Nisa Pamesty Rahma Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Alfia Magfirona Alfiyani, Lina Ali Djunaedi Amalia, Ayu Rizki Amanda Mega Putri, Amanda Mega ANGELA MARIANA LUSIASTUTI Anggoro, Agung Doni Anggun Putriani Situmorang, Anggun Putriani Aninditia Sabdaningsih Anisa Dwiaryani Latifah Annisa Oktafianti Nurlatifah, Annisa Oktafianti Aprilia Dwi Indriani Arifin Arifin Arum Almuaromah, Dita Asep Akmal Aonullah Astri Pujiati Aulia Resty Wijayanti Aulia, Annisa Syahida Ayi Santika Ayu Wulandari Azhar, Nuril Bosma, Roel Bosma, Roel H. Briliani Ayu Wardani Budi Setiawan Buyung Junaidin, Buyung Caesa, Genio Chairulina Pitrianingsih CHONDRORESMI BANOR FAWWAZ Chyntia Arindita Dani Indrarini David Panca Wijaya, David Panca Desrina Desrina Dewi Nurhayati Dhani Mutiari DIAH AYU SATYARI UTAMI Dian Ratna Sari Dian Wijayanto Diana Chilmawati Diana Chilmawati Diana Rachmawati Dicky Harwanto Ditha Cahyaningrum Ditha Febriana Dewanti Nineung Edward Raharja, Edward Endah Setyowati Eni Ashfa Ashofa Ervia Yudiati Fajar Basuki Famelia Meta Putri Fandy Malik Muhammad, Fandy Malik Fatian, Adella Spextania Ferdian Bagus Feriandika Fifiana Zulaekah Fitriadi, Ren Gina Saptiani Haeruddin Haeruddin Hasna, Salma Khoironnida Hasyim Asyari Ika Puspitasari Indah Febry Hastari Intan Eska Amalia Syahida Istiyanto Samidjan Jery, Jery Johannes Hutabarat Jokosisworo Jokosisworo Karim, Murni Kewa, Kristofora Karolina Khuzaimah, Ima Siti Kurniawan Kurniawan Laksono Trisnantoro Lestari Lakhsmi Widowati Lilik Maslukah Lilik Setiyaningsih Linuwih Aluh Prastiti Lukman Anugrah Agung Lukman Lukman M. Yogi Riyantama Isjoni Marwenni Siregar, Marwenni Milza Apriliani, Milza Mita Umiliana, Mita Monica Nanda Muchtar muchtar Muhammad Burhan Mukhlisin, Latutik Nailil Muna Nida Qolbi Salma Rochani Noor Alis Setiyadi Nur Aklis Nur Annisa Nuri Nia Yanti, Nuri Nia Nurul Hidayati Ocky Karna Radjasa Panji Yusroni Anwar Prabowo, Anggit Bayu Pramudita Apriliyanti Prayitno, S. Budi Pungki Nanda Pratama Purwanto Purwanto Pusaka, Semerdanta R. Dewi Dharina Nurjannah Rahman, Nuril Endi Rahmawati, Amelia Rahmi Gusti Darma Raynol Simorangkir Rensiga Rintan Bunga Sari Restiana Ariyati Restiana Wisnu Ariyati Rini, Endah Setyo Ristiawan Agung Nugroho Rohita Sari Rosa Amalia Rosalina Safitri Rusydina Qamarul Salikin S. Budi Prayitno Sahala Hutabarat Sarastiti, Siwi Schrama, Johan Sekar Ayu Chairunnisa Seto Windarto Setyo Putro Rahmanto Setyowati, Suryaning Siti Nurjanah Siti Ziyadaturrohmah Siwi Hartanti Slamet B Prayitno Slamet Budi Prayitno slamet budi prayitno Soedibya, Petrus Hary Tjahja Sri Hastuti Sri Nurchayati Sri Nuryati Sri Rejeki Subagiyo Subagiyo Subandiyono Subandiyono Subroto Subroto Sulisyaningrum Sulisyaningrum Sumini Sumini, Sumini Suminto , Suminto - Suminto Suminto Suminto Suminto Suminto Suminto Suminto Suminto Suradi Wijaya Saputra Tita Elfitasari Titik Susilowati Tri Mulyadi Trienes, Yoni W. Widiatmoko Wiji Utami, Wiji Wijianto Wijianto Wisnu Widyantoro Wiyadi Yelliana Fatmawati Suwarno Yohanes Kristiawan Artanto