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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia MEDIA KONSERVASI Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Lingkungan Indonesian Journal of Applied Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu Komputer dan Agri-Informatika Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Penelitian Hasil Hutan JURNAL PEMULIAAN TANAMAN HUTAN JURNAL PERBENIHAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) International Journal of Advances in Intelligent Informatics JURNAL BIOLOGI INDONESIA ZOO INDONESIA Kinetik: Game Technology, Information System, Computer Network, Computing, Electronics, and Control Indonesian Journal of Chemistry Forest and Society Jurnal Ilmu dan Teknologi Kayu Tropis Journal of Sylva Indonesiana Jurnal Penelitian Hasil Hutan Zoo Indonesia JURNAL HUTAN TROPIS Journal Of Sustainability Perspectives Jurnal Komputer Teknologi Informasi Sistem Komputer (JUKTISI) Jurnal Ilmu Kehutanan Journal of Sustainability Perspectives Policy Brief Pertanian, Kelautan, dan Biosains Tropika Indonesian Journal of Forestry Research Jurnal Penelitian Hasil Hutan

ISKANDAR ZULKARNAEN SIREGAR

Department Of Silviculture, Faculty Of Forestry, Bogor Agricultural University, Academic Ring Road Campus IPB Dramaga, PO Box 168, Bogor 16680, Indonesia

Author-ID : 381830

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Public Health Social Sciences Veterinary Other

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GENETIC VARIATION, HERITABILITY AND CORRELATION BETWEEN RESIN PRODUCTION CHARACTER OF Pinus merkusii HIGH RESIN YIELDER (HRY) IN CIJAMBU SEEDLING SEED ORCHARD (SSO) Iswanto, Arida Susilowati; Supriyanto, Supriyanto; Siregar, Iskandar Z.; Wahyudi, Imam; Corryanti, Corryanti
BIOTROPIA Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.2.257

Selection high resin candidate P.merkusii is a series of breeding activities to get high resin production trait of pine trees.Â In selection process of candidate tress, genetic diversity playsÂ very important role because higher genetic diversity will give higher opportunity to get source of genes for improved trait. Selection activities of high resin yielder candidates were started Â in 2006 through a series of survey activity and morphological identification of candidate trees with high resin production. Specific information about traits genetically related to resin production in Cijambu Seedling Seed Orchard is still not determined yet , although based on resin distribution trend this SSO has the highest mean of resin production. Â In this study, progenies planted in 1978 -1983 were evaluated to determine the effectiveness of previous selection, as well as the value of heritability and phenotypic traits that allegedly related with resin production. The results showed high value of coefficient genetic variation (CVG:16.08%), narrow sense heritability values for resin production trait (0.65) and resemble with previous research. This indicates that genetic factor was Â dominant for resin production and selection activities has been done for 30 years was effective to get high yielder superior candidate.Â Bark thickness, crown length and stem diameter trait was positively significant correlated to resin production, whereas level of pests and diseases was negatively significant correlated to resin production, it is possible that Â the trait related with lighting conditions that supporting photosynthesis process and number of resin duct in the wood. Â Key words: Selection, high resin yielder, P. merkusii, variation, heritability,phenotypic

ANATOMICAL CHARACTERS OF Aquilaria microcarpa INTERACTING WITH Fusarium sp. Siburian, Rima HS; Siregar, Ulfah J.; Siregar, Iskandar Z.; Santoso, Erdy; Wahyudi, Imam
BIOTROPIA Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013
Publisher : SEAMEO BIOTROP

Aquilaria microcarpa is one of agarwood-producing plants, Interaction of pathogenic fungi may cause physiological changes in plants that have an impact on visual changes in cells, tissues or organs of plants. This study aims to determine differences in the anatomical characters of Aquilaria microcarpa that have been inoculated with Fusarium sp. Observations of anatomical characters showed that traits of inoculated and un-inoculated Aquilaria microcarpa in general are partly different. The difference was found in wood color, odor/aroma, the pores of wood and leather inserted visible. Chemical compounds contained in the plant interact with fusarium was Elemol, Baimuxinal, 3-phenyl-2-butanone, chromen-4-one.

POPULATION GENETICS OF THE CRITICALLY ENDANGERED SPECIES Dipterocarpus littoralis Blume (Dipterocarpaceae) ENDEMIC ON NUSAKAMBANGAN ISLAND, INDONESIA Dwiyanti, Fifi Gus; Harada, Ko; Siregar, Iskandar Zulkarnaen; Kamiya, Koichi
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Background Dipterocarpus littoralis Blume is a critically endangered dipterocarp species found only on Nusakambangan Island, Central Java, Indonesia. Patterns of genetic diversity and population genetic structure of adults and saplings in two extant populations (Kali Jati and Solok Besek) were estimated using ten microsatellite markers. Results A total of 39 alleles was found, with two and four alleles being unique in adult and sapling populations, respectively. Allelic richness and heterozygosity was similar between adult (Ar = 3.00; He = 0.423) and sapling (Ar = 3.25; He = 0.441) populations.Inbreeding coefficientsin saplingswere positive in both populations and statistically significant in Kali Jati, while those in adult populations were not significantly different from zero, indicating excessive inbreeding and selfing in the sapling populations. Genetic differentiation of the sapling populations (FST = 0.036) was slightly lower than in the adult populations (0.050), but only significantly so for saplings. Conclusions This study revealed that D. littoralis has low genetic diversity in both adults and saplings. Similarly low values in allele richness and heterozygosity suggest that reductions of population size have been ongoing for long periods in this species. Significant genetic differentiation between sapling populations but not adult populations indicates that recent fragmentation is further accelerating the isolation process.

INTRASPECIFIC VARIATION ON EARLY GROWTH OF Neolamarckia cadamba Miq. IN PROVENANCE–PROGENY TESTS IN WEST JAVA PROVINCE, INDONESIA Sudrajat, Dede J; Nurhasybi, Nurhasybi; Siregar, Iskandar Z; Siregar, Ulfah J; Mansur, Irdika; Khumaida, Nurul
BIOTROPIA Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016
Publisher : SEAMEO BIOTROP

Genetic parameters for early growth of Neolamarckia cadamba, an indigenous species with potential as a source of timber wood, were estimated in open-pollinated provenance–progeny tests conducted at two sites in West Java Province, Indonesia. The experiment was arranged in a randomized complete block design with 12 provenances, 105 families, and five replications in four-tree row plots. Total height and root collar diameter were measured at 18 months of age. Significant differences among provenances and among families within provenances were observed for height and collar diameter at all sites, except for collar diameter among provenances at the Parungpanjang site. In general, the Garut (GSJ) provenance performed better than the Kualakencana (KKP) and Nusa Kambangan (NKJ) provenances at both sites. Evaluation of variance components at the two sites showed that the provenance effects (ranging from 0.5 to 1.7%) contributed more to the total variance than family-within-provenance effects (ranging from 0.4 to 0.6%). Genetic correlations between height and collar diameter were weak to moderate. Heritability estimates were low for all traits at Limbangan, whereas they were moderate at Parungpanjang. Estimated genetic gain for height and diameter based on proportional family selection of 0.30 was 0.13 and 0.18 at Limbangan, and 0.31 and 0.16 at Parungpanjang, respectively. Continued heritability measurement is required to obtain stable values. Stable heritability combined with family selection and within-family selection will improve genetic gain.

DNA BARCODE CHARACTERIZATION OF MISTLETOE INFESTATION IN TEAK CLONAL SEED ORCHARD (CSO) IN PADANGAN, EAST JAVA PROVINCE, INDONESIA Muttaqin, Zainal; Budi, Sri Wilarso; Wasis, Basuki; Corryanti, Corryanti; Siregar, Iskandar Zulkarnaen
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

For effective teak plantation management, early detection system in controlling teak mistletoe requires various basic information, including degree of infestation and accuracy of the species names. Mistletoe infestations in teak and mistletoe species name have been reported, but there are still problems in identifying the correct species or subspecies due to morphological similarity. The objective of this study was to clarify the species identity of hemi-parasitic mistletoe plants, which were found in teak Clonal Seed Orchard (CSO) in Padangan, East Java Province, Indonesia using DNA barcodes. Species identification of teak mistletoe based on leaf morphological characteristics and universal DNA barcode regions (i.e. matK and rbcL) were carried out. The results showed that the Canonical Discriminant Analysis (CDA) could differentiate Dendrophthoe pentandra and Macrosolen tetragonus based on leaf morphological characteristics. Variables having high correlation to distinguish both species were length of petiole, width of the widest leaf, number of secondary leaf veins, leaf base shape, aspect ratio, form factor and perimeter ratio of diameter. The results of DNA barcoding showed that the two DNA barcode regions presented good amplification and sequence results. Both DNA barcode regions successfully differentiated two species i.e. D. pentandra and M. tetragonus which belong to Loranthaceae family and have similar leaf morphological characteristics. Those regions were also able to identify Viscum articulatum and other species belonging to Santalaceae family. These results suggested that the two DNA regions could become recommended universal DNA barcode for identifying teak mistletoe.

GENETIC VARIATION OF TEAK MISTLETOE (Dendrophthoe pentandra (L.) Miq.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS Muttaqin, Zainal; Budi, Sri Wilarso; Wasis, Basuki; Siregar, Iskandar Zulkarnaen; Corryanti, Corryanti
BIOTROPIA Vol. 27 No. 2 (2020): BIOTROPIA Vol. 27 No. 2 August 2020
Publisher : SEAMEO BIOTROP

Mistletoes are hemiparasitic macroparasite plants that interfere with trees and other wild plants in nutrient acquisition. As the plant has low leaf water potential, it draws water from teak wood tissues during the deciduous stage of the teak host, thereby killing the twigs and eventually the teak tree. Mistletoes are also key players in plant diversity. Therefore, the mistletoe population needs to be regulated not only as a parasite but also as a keystone species affecting biodiversity. Knowledge scarcity on the status of mistletoes includes their genetic variation. Hence, the purpose of this study is to analyze the level of genetic variation of teak mistletoe (Dendrophthoe pentandra) using RAPD markers. At the Padangan teak Clonal Seed Orchard (CSO), leaf samples were randomly collected from three layers of the mistletoe crown (upper, middle, and below) taken from five host teak trees randomly selected from each of the sub-observation measure plots (OMP). Four OMP units inside the observation sample plots (OSP) (n = 3, 50 × 50 m) at different levels of infestation (light, moderate, and heavy) were established. Analysis of genetic variation and genetic distance of mistletoes across different crown layers was conducted using RAPD markers. The leaf samples from crown layers UU (upper crown, subsection upper), UM (upper crown, subsection middle), and UB (upper crown, subsection below), including U (upper crown), had significantly greater genetic variation (He = 0.181–0.255) than those from the M layer (middle crown, He = 0.227) and the B layer (below crown, He = 0.114). Furthermore, the greatest genetic distance occurred between mistletoes in the UB and B crown layers (0.310), whereas the shortest genetic distance occurred between mistletoes of the UU and UM layers in the upper crown (0.038). Practical implications of the low genetic variation include the control of D. pentandra infestation by restricting its population so that the Perhutani State-Owned Forestry Enterprise can maintain damage levels below the economic threshold.

Optimizing DNA Extraction Methods from Leaf and Wood Tissues to Support Dipterocarp Conservation and Sustainable Forest Management Arrofaha, Nawwall; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Adinugroho, Wahyu Catur; Siregar, Iskandar Zulkarnaen; Kamal, Irsyad; Syaputra, Dhika; Salamah, Andi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 1 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.1.13

Dipterocarpaceae are economically important, contributing over 85% of Indonesia's timber exports. However, this crucial resource is increasingly threatened by illegal logging, habitat destruction, and the illegal timber trade, which jeopardize dipterocarp population. Furthermore, conservation efforts utilizing genetic and forensic techniques often encounter substantial challenges due to the complexities in DNA extraction protocol. To address this, the study aimed to enhance the efficiency of DNA extraction methodologies by comparing two methods: the modified cetyltrimethylammonium bromide (CTAB) and the Genomic DNA Mini Kit (Plant) from Geneaid Biotech Ltd. The research focused on leaf and wood samples from two species, spesifically Rubroshorea leprosula (Miq.) P.S.Ashton & J.Heck and Shorea laevis Ridl. For each of these species, five leaf and five wood samples were extracted using both methods. The quality of the DNA extraction was evaluated using electrophoresis and quantified with a Qubit fluorometer. Higher DNA concentrations were obtained with the modified CTAB method compared to the GeneAid kit for both R. leprosula and S. laevis, particularly in leaf tissue. The GeneAid kit consistently exhibited low DNA yield efficiency compared to the modified CTAB method for both species. Additionally, PCR amplification of both leaf and wood samples confirmed that the extracted DNA was suitable formolecular analyses. These findings not only contributed to laboratory applications but also served as practical tools for species identification and genetic conservation for sustainable forest management and law enforcement.

Penentuan Kerapatan Massa Tajuk Pohon Decurrent Berdasarkan Analisis Keragaan Fisik Aini, Siti Churotul; Ulfa Adzkia; Agus Buono; Siregar, Iskandar Zulkarnaen; Lina Karlinasari
Jurnal Ilmu Kehutanan Vol 20 No 1 (2026): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jik.v20i1.22134

Self-weight calculation was identified as an essential step in tree structural analysis. In practice, this calculation included estimating stem weight based on wood density, while crown density was expressed as a percentage of crown area. However, previous research had not provided explicit methodologies for determining crown density as a function of crown mass and volume. Therefore, this research aimed to establish a proper method and corresponding conversion factors for translating area-based crown density estimates to those derived from crown weight and volume. A total of 15 healthy decurrent trees were selected to assess crown volume and density through physical attribute analysis. The new crown area density was calculated after pruning, where all pruned crown biomass was collected and weighed to determine crown mass. Mathematical analyses were developed to convert crown density values. The results showed that decurrent trees had a mean crown density of approximately 2.95 kg/m3, exceeding the value reported in reference research for excurrent trees (1.9 kg/m3). Since this research focused on tropical tree species, the results could serve as a reference for subsequent research on tropical tree structural characteristics.

Molecular taxonomy via DNA barcodes for species identification in selected genera of Fabaceae Wardani, I Gusti Ayu Kusuma; Amandita, Fitri Yola; Moura, Carina Carneiro de Melo; Gailing, Oliver; Siregar, Iskandar Z
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 12 No 1 (2022): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.12.1.112-122

Fabaceae is an invaluable plant family with considerable ecological and economic importances for example as food sources, bio-fertilizer, and medicinal plants. However, Fabaceae has been overexploited in Indonesia and several species belong to this family are critically endangered. Due to morphological similarity, rapid and accurate identification of Fabaceae species is essential to support its conservation efforts. Nowadays, species identification through DNA barcoding has become an effective taxonomic classification tool. This study was aimed to evaluate the effectiveness of chloroplast markers, matK and rbcL and the combination (matK+rbcL), as DNA barcodes for the identification of seleted genera in Fabaceae. The result showed that matK+rbcL and matK had the highest level of identification (90% and 82.05%) of the investigated genera, respectively. Additionally, matK had the highest mean of interspesific distance (0.134) and intraspecific distance (0.003). The combined barcode matK+rbcL had the highest correct identification rate when comparing the morphological with molecular identification. Furthermore, the best phylogenetic tree was obtained using Neighbor Joining method. Based on the overall performance, matK and matK+rbcL were the best barcodes for the selected genera in this study.

Co-Authors . Yulianti Aam Aminah Aam Aminah Abdul Munif Adinugroho, Wahyu Catur Aditya Nugroho Adzkia, Ulfa Agus Astho Pramono Agus Buono Agus Purwito Agus Wahyudi Aini, Siti Churotul Alami, Tegar Alfi Hudatul Karomah Ali Mukmin Amandita, Fitri Yola Aminah . Andi Salamah Andi Sukendro Andry Indrawan Ani Suryani Ani Suryani Anita, Vilda Puji Dini Arida Susilowati Iswanto Arif Satria Arrofaha, Nawwall Atok Subiakto Ayyasy, Yahya Baiquni Rangkuti, Ahmad Basuki Wasis Budi Tjahjono Cartealy, Imam Civi Cecep Kusmana Corryanti Corryanti Dede J Sudrajat Dede J. Sudrajat Dede J. Sudrajat Deden Derajat Matra Detty Sumiyati Dida Syamsuwida Dida Syamsuwida Dien Atin Boritnaban Divi Handoko Dodik Ridho Nurrochmat Edje Djamhuri Endah R Palupi Endah Retno Palupi Erdy Santoso Ervizal A.M. Zuhud Ervizal AMZU Eryna Elfasari Rangkuti Fifi Gus Dwiyanti Fifi Gus Dwiyati Fredisa, Yoga Gailing, Oliver Harisson, Rhett D. Hartati Hartati Hartati, N Sri Henti Hendalastuti R., Henti Hendalastuti Henti Rosdayanti Henti Rosdayanti, Henti IBNUL QAYIM Imam Wahyudi Indrawan, Imam Wahyudi IPB, BPKB Irdika Mansur Irsyad Kamal Ishak Yassir Istomo . Karlisa Priandana Kaswanto, Regan L. Kissinger Kissinger Ko Harada Koichi Kamiya Kosasih, Akhmad Kustiyarini, Nur Fadila KUSUMADEWI SRI YULITA Laswi Irmayanti Laswi Irmayanti, Laswi Latifah K. Darusman Latifah K. Darusman Lina Karlinasari Lutfy Abdulah Majiidu, Muhammad Matra, Deden Derajat Mohamad Rafi Moura, Carina Carneiro de Melo Mr. Kissinger Muhammad Majiidu Muhammad Rafi Muttaqin Muharam, Karima Fauziah Murdaningsih Haeruman K. Muttaqin, Muhammad Rafi Nasution, Tegar Alami Nelly Anna Nurhasybi Nurhasybi Nurul Khumaida Nyoto Santoso Prijanto Pamoengkas Putra, Heriansyah Rachmat, Henti Hendalastuti Rahila Junika Tanjungsari Rahman, Mohamad Miftah Rangkuti, Reyhan Abdillah RIKA RAFFIUDIN rima siburian Rina Mardiana Septaningsih, Dewi Anggraini Silalahi, Mangarah Siregar, Ulfa Juniarti Siregar, Ulfah J Situmorang, Rizki Andista Pandapotan Slamet, Alim Setiawan Sri Wilarso Budi Supatmi Supatmi Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Suria Darma Tarigan Suryanto Suryanto Suseno Amien Susila, Susila Syaiful Anwar Syamsidah Rahmawati, Syamsidah Syaputra, Dhika Tedi Yunanto Tedi Yunanto Ulfa Adzkia Ulfah Juniarti Siregar Ulfah, Mariyana Ulfah, Mariyana Utami, Anisa Dwi Wardani, I Gusti Ayu Kusuma Wati, Ridha Wijayanto, Nurheni WINDRA PRIAWANDIPUTRA, WINDRA Wisnu Ananta Kusuma Yayat Hidayat Yulianti Bramasto Zainal Muttaqin

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