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Genetic Variation, Heritability and Correlation between Resin Production Character of Pinus merkusii High Resin Yielder (HRy) in Cijambu Seedling Seed Orchard (SSO) Iswanto, Arida Susilowati; Supriyanto, Supriyanto; Siregar, Iskandar Z.; Wahyudi, Imam; Corryanti, Corryanti
BIOTROPIA Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.2.257

Abstract

Selection high resin candidate P.merkusii is a series of breeding activities to get high resin production trait of pine trees.  In selection process of candidate tress, genetic diversity plays  very important role because higher genetic diversity will give higher opportunity to get source of genes for improved trait. Selection activities of high resin yielder candidates were started  in 2006 through a series of survey activity and morphological identification of candidate trees with high resin production. Specific information about traits genetically related to resin production in Cijambu Seedling Seed Orchard is still not determined yet , although based on resin distribution trend this SSO has the highest mean of resin production.  In this study, progenies planted in 1978 -1983 were evaluated to determine the effectiveness of previous selection, as well as the value of heritability and phenotypic traits that allegedly related with resin production. The results showed high value of coefficient genetic variation (CVG:16.08%), narrow sense heritability values for resin production trait (0.65) and resemble with previous research. This indicates that genetic factor was  dominant for resin production and selection activities has been done for 30 years was effective to get high yielder superior candidate.  Bark thickness, crown length and stem diameter trait was positively significant correlated to resin production, whereas level of pests and diseases was negatively significant correlated to resin production, it is possible that  the trait related with lighting conditions that supporting photosynthesis process and number of resin duct in the wood.  Key words: Selection, high resin yielder, P. merkusii, variation, heritability,phenotypic
Anatomical Characters of Aquilaria microcarpa Interacting with Fusarium sp Siburian, Rima HS; Siregar, Ulfah J.; Siregar, Iskandar Z.; Santoso, Erdy; Wahyudi, Imam
BIOTROPIA Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1409.897 KB) | DOI: 10.11598/btb.2013.20.2.258

Abstract

AbstractAquilaria microcarpa is one of agarwood-producing plants, Interaction of pathogenic fungi may cause physiological changes in plants that have an impact on visual changes in cells, tissues or organs of plants. This study aims to determine differences in the anatomical characters of Aquilaria microcarpa that have been inoculated with Fusarium sp. Observations of anatomical characters showed that traits of inoculated and un-inoculated Aquilaria microcarpa in general are partly different. The difference was found in wood color, odor/aroma, the pores of wood and leather inserted visible. Chemical compounds contained in the plant interact with fusarium was Elemol, Baimuxinal, 3-phenyl-2-butanone, chromen-4-one.
Population Genetics of the Critically Endangered Species Dipterocarpus littoralis Blume (Dipterocarpaceae) Endemic on Nusakambangan Island, Indonesia Dwiyanti, Fifi Gus; Harada, Ko; Siregar, Iskandar Zulkarnaen; Kamiya, Koichi
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17150.467 KB) | DOI: 10.11598/btb.2014.21.1.304

Abstract

Background Dipterocarpus littoralis Blume is a critically endangered dipterocarp species found only on Nusakambangan Island, Central Java, Indonesia. Patterns of genetic diversity and population genetic structure of adults and saplings in two extant populations (Kali Jati and Solok Besek) were estimated using ten microsatellite markers. Results A total of 39 alleles was found, with two and four alleles being unique in adult and sapling populations, respectively. Allelic richness and heterozygosity was similar between adult (Ar = 3.00; He = 0.423) and sapling (Ar = 3.25; He = 0.441) populations.Inbreeding coefficientsin saplingswere positive in both populations and statistically significant in Kali Jati, while those in adult populations were not significantly different from zero, indicating excessive inbreeding and selfing in the sapling populations. Genetic differentiation of the sapling populations (FST = 0.036) was slightly lower than in the adult populations (0.050), but only significantly so for saplings.Conclusions This study revealed that D. littoralis has low genetic diversity in both adults and saplings. Similarly low values in allele richness and heterozygosity suggest that reductions of population size have been ongoing for long periods in this species. Significant genetic differentiation between sapling populations but not adult populations indicates that recent fragmentation is further accelerating the isolation process.
Intraspecific Variation on Early Growth of Neolamarckia cadamba Miq. in Provenance-Progeny Tests in West Java Province, Indonesia Sudrajat, Dede J; Nurhasybi, Nurhasybi; Siregar, Iskandar Z; Siregar, Ulfah J; Mansur, Irdika; Khumaida, Nurul
BIOTROPIA Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1301.149 KB) | DOI: 10.11598/btb.2016.23.1.439

Abstract

Genetic parameters for early growth of Neolamarckia cadamba, an indigenous species with potential as a source of timber wood, were estimated in open-pollinated provenance–progeny tests conducted at two sites in West Java Province, Indonesia. The experiment was arranged in a randomized complete block design with 12 provenances, 105 families, and five replications in four-tree row plots. Total height and root collar diameter were measured at 18 months of age. Significant differences among provenances and among families within provenances were observed for height and collar diameter at all sites, except for collar diameter among provenances at the Parungpanjang site. In general, the Garut (GSJ) provenance performed better than the Kualakencana (KKP) and Nusa Kambangan (NKJ) provenances at both sites. Evaluation of variance components at the two sites showed that the provenance effects (ranging from 0.5 to 1.7%) contributed more to the total variance than family-within-provenance effects (ranging from 0.4 to 0.6%). Genetic correlations between height and collar diameter were weak to moderate. Heritability estimates were low for all traits at Limbangan, whereas they were moderate at Parungpanjang. Estimated genetic gain for height and diameter based on proportional family selection of 0.30 was 0.13 and 0.18 at Limbangan, and 0.31 and 0.16 at Parungpanjang, respectively. Continued heritability measurement is required to obtain stable values. Stable heritability combined with family selection and within-family selection will improve genetic gain.
DNA Barcode Characterization of Mistletoe Infestation in Teak Clonal Seed Orchard (CSO) in Padangan, East Java Province, Indonesia Muttaqin, Zainal; Budi, Sri Wilarso; Wasis, Basuki; Corryanti, Corryanti; Siregar, Iskandar Zulkarnaen
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2755.603 KB) | DOI: 10.11598/btb.2017.24.2.651

Abstract

For effective teak plantation management, early detection system in controlling teak mistletoe requires various basic information, including degree of infestation and accuracy of the species names. Mistletoe infestations in teak and mistletoe species name have been reported, but there are still problems in identifying the correct species or subspecies due to morphological similarity. The objective of this study was to clarify the species identity of hemi-parasitic mistletoe plants, which were found in teak Clonal Seed Orchard (CSO) in Padangan, East Java Province, Indonesia using DNA barcodes. Species identification of teak mistletoe based on leaf morphological characteristics and universal DNA barcode regions (i.e. matK and rbcL) were carried out. The results showed that the Canonical Discriminant Analysis (CDA) could differentiate Dendrophthoe pentandra and Macrosolen tetragonus based on leaf morphological characteristics. Variables having high correlation to distinguish both species were length of petiole, width of the widest leaf, number of secondary leaf veins, leaf base shape, aspect ratio, form factor and perimeter ratio of diameter. The results of DNA barcoding showed that the two DNA barcode regions presented good amplification and sequence results. Both DNA barcode regions successfully differentiated two species i.e. D. pentandra and M. tetragonus which belong to Loranthaceae family and have similar leaf morphological characteristics. Those regions were also able to identify Viscum articulatum and other species belonging to Santalaceae family. These results suggested that the two DNA regions could become recommended universal DNA barcode for identifying teak mistletoe.
Genetic Variation of Teak Mistletoe (Dendrophthoe pentandra (L.) Miq.) Based on Random Amplified Polymorphic DNA (RAPD) Markers Muttaqin, Zainal; Budi, Sri Wilarso; Wasis, Basuki; Siregar, Iskandar Zulkarnaen; Corryanti, Corryanti
BIOTROPIA Vol. 27 No. 2 (2020): BIOTROPIA Vol. 27 No. 2 August 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.519 KB) | DOI: 10.11598/btb.2020.27.2.1211

Abstract

Mistletoes are hemiparasitic macroparasite plants that interfere with trees and other wild plants in nutrient acquisition. As the plant has low leaf water potential, it draws water from teak wood tissues during the deciduous stage of the teak host, thereby killing the twigs and eventually the teak tree. Mistletoes are also key players in plant diversity. Therefore, the mistletoe population needs to be regulated not only as a parasite but also as a keystone species affecting biodiversity. Knowledge scarcity on the status of mistletoes includes their genetic variation. Hence, the purpose of this study is to analyze the level of genetic variation of teak mistletoe (Dendrophthoe pentandra) using RAPD markers. At the Padangan teak Clonal Seed Orchard (CSO), leaf samples were randomly collected from three layers of the mistletoe crown (upper, middle, and below) taken from five host teak trees randomly selected from each of the sub-observation measure plots (OMP). Four OMP units inside the observation sample plots (OSP) (n = 3, 50 × 50 m) at different levels of infestation (light, moderate, and heavy) were established. Analysis of genetic variation and genetic distance of mistletoes across different crown layers was conducted using RAPD markers. The leaf samples from crown layers UU (upper crown, subsection upper), UM (upper crown, subsection middle), and UB (upper crown, subsection below), including U (upper crown), had significantly greater genetic variation (He = 0.181–0.255) than those from the M layer (middle crown, He = 0.227) and the B layer (below crown, He = 0.114). Furthermore, the greatest genetic distance occurred between mistletoes in the UB and B crown layers (0.310), whereas the shortest genetic distance occurred between mistletoes of the UU and UM layers in the upper crown (0.038). Practical implications of the low genetic variation include the control of D. pentandra infestation by restricting its population so that the Perhutani State-Owned Forestry Enterprise can maintain damage levels below the economic threshold.
Co-Authors . Yulianti Aam Aminah Aam Aminah Abdul Munif Aditya Nugroho Adzkia, Ulfa Agus Astho Pramono Agus Buono Agus Purwito Agus Wahyudi Alami, Tegar Alfi Hudatul Karomah Ali Mukmin Aminah . Andi Sukendro Andry Indrawan Ani Suryani Ani Suryani Anita, Vilda Puji Dini Arida Susilowati Iswanto Arif Satria Arrofaha, Nawwall Atok Subiakto Ayyasy, Yahya Baiquni Rangkuti, Ahmad Basuki Wasis Budi Tjahjono BUDI TJAHJONO Cartealy, Imam Civi Cecep Kusmana Corryanti Corryanti Dede J Sudrajat Dede J. Sudrajat Dede J. Sudrajat Deden Derajat Matra Detty Sumiyati Dida Syamsuwida Dida Syamsuwida Dien Atin Boritnaban Divi Handoko Dodik Ridho Nurrochmat Edje Djamhuri Endah R Palupi Endah Retno Palupi Erdy Santoso Ervizal A.M. Zuhud Ervizal AMZU Eryna Elfasari Rangkuti Fifi Gus Dwiyanti Fifi Gus Dwiyati Fredisa, Yoga Harisson, Rhett D. Hartati Hartati Hartati, N Sri Henti Hendalastuti R., Henti Hendalastuti Henti Rosdayanti Henti Rosdayanti, Henti IBNUL QAYIM Imam Wahyudi Indrawan, Imam Wahyudi IPB, BPKB Irdika Mansur Irsyad Kamal Ishak Yassir Istomo . Karlisa Priandana Kaswanto, Regan L. Kissinger Kissinger Ko Harada Koichi Kamiya Kosasih, Akhmad Kustiyarini, Nur Fadila KUSUMADEWI SRI YULITA Laswi Irmayanti Laswi Irmayanti, Laswi Latifah K. Darusman Latifah K. Darusman Lina Karlinasari Lutfy Abdulah Majiidu, Muhammad Matra, Deden Derajat Mohamad Rafi Mr. Kissinger Muhammad Majiidu Muhammad Rafi Muttaqin Muharam, Karima Fauziah Murdaningsih Haeruman K. Muttaqin, Muhammad Rafi Nasution, Tegar Alami Nelly Anna Nurhasybi Nurhasybi Nurul Khumaida Nyoto Santoso Prijanto Pamoengkas Putra, Heriansyah Rachmat, Henti Hendalastuti Rahila Junika Tanjungsari Rahman, Mohamad Miftah Rangkuti, Reyhan Abdillah RIKA RAFFIUDIN rima siburian Rina Mardiana Septaningsih, Dewi Anggraini Silalahi, Mangarah Siregar, Ulfa Juniarti Siregar, Ulfah J Situmorang, Rizki Andista Pandapotan Slamet, Alim Setiawan Sri Wilarso Budi Supatmi Supatmi Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Suria Darma Tarigan Suryanto Suryanto Suseno Amien Susila, Susila Syaiful Anwar Syamsidah Rahmawati, Syamsidah Tedi Yunanto Tedi Yunanto Ulfah Juniarti Siregar Ulfah, Mariyana Ulfah, Mariyana Utami, Anisa Dwi Wati, Ridha Wijayanto, Nurheni WINDRA PRIAWANDIPUTRA, WINDRA Wisnu Ananta Kusuma Yayat Hidayat Yulianti Bramasto Zainal Muttaqin