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All Journal Buletin Peternakan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Pastura; Journal Of Tropical Forage Science Jurnal Veteriner Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Ilmu dan Industri Peternakan Biotropika Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production Jurnal Peternakan (Jurnal of Animal Science) Conference on Innovation and Application of Science and Technology (CIASTECH) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Seminar Nasional Lahan Suboptimal Jurnal Ilmiah Inovasi Jurnal Ilmiah Fillia Cendekia JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan Jurnal Ilmu Peternakan Terapan Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Sains dan Teknologi Peternakan Indonesian Journal of Applied Research (IJAR) Jurnal Nukleus Peternakan Sibatik Journal : Jurnal Ilmiah Bidang Sosial, Ekonomi, Budaya, Teknologi, Dan Pendidikan Livestock and Animal Research Prosiding Seminar Nasional Mini Riset Mahasiswa Journal of Livestock and Animal Health (JLAH) Jurnal Peternakan Borneo Jurnal Agripet Animal Production Journal of Agriprecision & Social Impact
Osfar Sjofjan
Animal Husbandry Faculty, Brawijaya University-Malang
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Potential Antibacterials Compounds of Lactic Acid Bacteria (LAB) from Quail Intestine (Coturnix japonica) in Inhibition Growth of Escherichia coli and Salmonella typhimurium Afdora, Pupimadita Tizar; Ardiyati, Tri; Sjofjan, Osfar; Kalsum, Umi
Journal of Tropical Life Science Vol 1, No 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Quail (Coturnix japonica) is a bird that have high protein content, but vulnerable to digestive diseases. The purpose of this research was to determine the ability of antibacterial compounds of LAB from intestinal quail origin in bacterial growth inhibition test. This research used Completely Randomized Design (CRD) with variable concentrations of Cell Free Supernatant (CFS) for 10, 20, 30, 40, 50, and 60% and bacterial pathogens of the digestive tract of quail (S. typhimurium, E. coli of human origin, and E. coli of bird origin) by using the Minimum inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The data obtained were analyzed by Analysis of Variance (ANOVA). The results obtained showed that the bacterium Lactobacillus fermentum and L. salivarius derived from quail intestine can produce antibacterial compounds that could inhibit the growth of Salmonella typhimurium, Escherichia coli (human), and E. coli (bird). Minimum concentration of the addition of CFS from L. fermentum in inhibiting the growth of tested bacteria was 30% for S. typhimurium, 30% for E. coli (human), and 20% for E. coli (bird). While the addition of CFS minimum concentration of L. salivarius in inhibiting the growth of tested bacteria was 20% for S. typhimurium, 20% for E. coli (human), and 10% for E. coli (bird). Keywords: Antibacterials, Lactic Acid Bacteria (LAB), Escherichia coli, Quail, and Salmonella typhimurium.
Potential Antibacterials Compounds of Lactic Acid Bacteria (LAB) from Quail Intestine (Coturnix japonica) in Inhibition Growth of Escherichia coli and Salmonella typhimurium Pupimadita Tizar Afdora; Tri Ardiyati; Osfar Sjofjan; Umi Kalsum
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Quail (Coturnix japonica) is a bird that have high protein content, but vulnerable to digestive diseases. The purpose of this research was to determine the ability of antibacterial compounds of LAB from intestinal quail origin in bacterial growth inhibition test. This research used Completely Randomized Design (CRD) with variable concentrations of Cell Free Supernatant (CFS) for 10, 20, 30, 40, 50, and 60% and bacterial pathogens of the digestive tract of quail (S. typhimurium, E. coli of human origin, and E. coli of bird origin) by using the Minimum inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The data obtained were analyzed by Analysis of Variance (ANOVA). The results obtained showed that the bacterium Lactobacillus fermentum and L. salivarius derived from quail intestine can produce antibacterial compounds that could inhibit the growth of Salmonella typhimurium, Escherichia coli (human), and E. coli (bird). Minimum concentration of the addition of CFS from L. fermentum in inhibiting the growth of tested bacteria was 30% for S. typhimurium, 30% for E. coli (human), and 20% for E. coli (bird). While the addition of CFS minimum concentration of L. salivarius in inhibiting the growth of tested bacteria was 20% for S. typhimurium, 20% for E. coli (human), and 10% for E. coli (bird). Keywords: Antibacterials, Lactic Acid Bacteria (LAB), Escherichia coli, Quail, and Salmonella typhimurium.
Screening of Cellulolytic Bacteria from Sugarcane Waste (Bagasse) and Optimization of Cellulase Activity as Animal Feed: Screening of Cellulolytic Bacteria from Sugarcane Waste (Bagasse) Ramadhani, Sulistya Ika; Ardyati, Tri; Sjofjan, Osfar
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.19

Abstract

The Sugar Factory of Tjoekir Jombang is one of the sugar factories located in Jombang, East Java under PT Perkebunan Nusantara X. Sugarcane waste, also known as bagasse causes pollution of the environment. Some microorganisms are able to degrade cellulose-containing bagasse, because of cellulolytic enzymes produced by cellulolytic bacteria. This study aims to obtain cellulolytic bacteria isolates, screen the highest cellulolytic activity, identify the highest cellulolytic activity isolates, and optimize conditions (pH and temperature) for the highest cellulase activity. Cellulolytic bacteria from bagasse were grown on a medium containing 1% CMC. Several additional minerals were other than those in the CMC medium. The cellulase activity was assayed semi-quantitatively with the addition of 0.1% Congo red and quantitatively using the 3,5-Dinitro Salicylic Acid (DNS) method. Bacterial isolates with high cellulolytic activity were identified based on the 16S rDNA sequence. This research obtained 20 bacterial isolates, where isolate A1T4 had the highest cellulolytic index of 1.18 mm. Measurement of cellulase activity using the DNS method showed that isolate A2T2 had the highest cellulase activity of 2.19 U/mL. Hemolysis assay showed that from 12 isolates, only two isolates have γ-hemolysis activity (isolates A1T6 and A3T3). Those isolates were optimized in a CMC broth medium with temperatures of 30°C and 37°C and pH 5 and pH 6 to produce the highest cellulase activity. Isolate A1T6 and A3T3 were grown optimally at 30oC and pH 6. Isolate A1T6 was identified as Citrobacter amalonaticus with a similarity of 99.80%, and isolate A3T3 was identified as Pseudomonas mendocina with a similarity of 98.83%.
Screening of Cellulolytic Bacteria from Sugarcane Waste (Bagasse) and Optimization of Cellulase Activity as Animal Feed: Screening of Cellulolytic Bacteria from Sugarcane Waste (Bagasse) Ramadhani, Sulistya Ika; Ardyati, Tri; Sjofjan, Osfar
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.19

Abstract

The Sugar Factory of Tjoekir Jombang is one of the sugar factories located in Jombang, East Java under PT Perkebunan Nusantara X. Sugarcane waste, also known as bagasse causes pollution of the environment. Some microorganisms are able to degrade cellulose-containing bagasse, because of cellulolytic enzymes produced by cellulolytic bacteria. This study aims to obtain cellulolytic bacteria isolates, screen the highest cellulolytic activity, identify the highest cellulolytic activity isolates, and optimize conditions (pH and temperature) for the highest cellulase activity. Cellulolytic bacteria from bagasse were grown on a medium containing 1% CMC. Several additional minerals were other than those in the CMC medium. The cellulase activity was assayed semi-quantitatively with the addition of 0.1% Congo red and quantitatively using the 3,5-Dinitro Salicylic Acid (DNS) method. Bacterial isolates with high cellulolytic activity were identified based on the 16S rDNA sequence. This research obtained 20 bacterial isolates, where isolate A1T4 had the highest cellulolytic index of 1.18 mm. Measurement of cellulase activity using the DNS method showed that isolate A2T2 had the highest cellulase activity of 2.19 U/mL. Hemolysis assay showed that from 12 isolates, only two isolates have γ-hemolysis activity (isolates A1T6 and A3T3). Those isolates were optimized in a CMC broth medium with temperatures of 30°C and 37°C and pH 5 and pH 6 to produce the highest cellulase activity. Isolate A1T6 and A3T3 were grown optimally at 30oC and pH 6. Isolate A1T6 was identified as Citrobacter amalonaticus with a similarity of 99.80%, and isolate A3T3 was identified as Pseudomonas mendocina with a similarity of 98.83%.
Co-Authors - Aulani’Am A.P Rahayu Abd. Rasyid Syamsuri Achmad Bagus Adhiluhung Mardhotillah Achmanu . Adam E. Saputra Addharul Muttaqin Adelina Ari Hamiyanti Adharul Mutaqin Adharul Mutaqin Aditias Nabil Khoirunisa Adli, D. N. Adli, Danung Nur Afdora, Pupimadita Tizar Agus Susilo Ahmad Nur Musyarrof Al Akrom Ahzami, Abdurahhman Aini, Baiq Widya Rahmatul Ajeng Kusumaningtyaswati Akhmad Zaenal Wijaya Amini, Khairuni Anisa Ramadhani Antonius Suwanto Aqnes Suryani Asri Nurul Huda Awang Tri Satria Ayu Damayanti B. Sundu Badat Muwakhid Bintang Putri Churriyah, Rosyidatul Cindy A. Damayanti Cindy Audina Damayanti Danial Mukhtar Husada Derana Tri Mustikawatie Dhika Yonika Primacitra Diah Kartika Sari Didik Nur Edi Dina Marliana Djalal Rosyidi Djunaidi, Irfan dos Santos, Abilio Dwi Kiat Puspitasari Dyah Lestari Yulianti Dyah Lestari Yulianti Edhy Sudjarwo Edhy Sudjarwo Edhy Sudjarwo Edhy Sudjarwo Edi Wahyu Satria Edi, Didik Nur Eko Widodo Eny Sri Widyastuti Esti Puspitasari Faizal Andri Farela Adhana Muflikhien Farida Kusuma Astuti Febri Puska Padang Filoza Marwi Filoza Marwi Firmansyah, Angga Fitri Wahyuni Fitria Tridyana Putri Hadi, Tasya Anggraini Sukma Haryaka Tisti Sadara Heli Tistiana Heni Setyo Prayogi Hertamawati, Rosa Tri Hudan Allama Ika Widiyawati Ilmi Sholichatunnisa Ilmiatus Sholichatunnisa Indah Puspita Sari Irfan Djunaidi Irfan Djunaidi Irfan H. Djunaidi Irfan H. Djunaidi Jihana, Amalia Iffah Kasri, Kasri Khaerunnisa Firdaus Sadikin Kuncoro Kuncoro Kuncoro, Kuncoro Kurniawan, Muhammad Iqbal Laila Nur Rohma Lestari, Suci Puji Lestariningsih Lestariningsih Lestariningsih Lestariningsih Lilik Eka Radiati Ludovika Jessica Virginia M Halim Natsir M Halim Natsir M. H. Natsir M. Halim Natsir Mariani Jesika Widiawati Mashudi Mashudi Muhammad A. Y. Harahap Muhammad Andika Yudha Harahap Muhammad Andika Yudha Harahap Muhammad H. Natsir Muhammad Halim Natsir Muhammad Halim Natsir Muhammad Izzuddiyn Muharlien Muharlien Muharlien Muharlien Nanang Febrianto Natasha Lenzky Mutia Nur Hidayah Nuraini Nadzifah Nurul Jannah, Saadatin Obbi Firmansyah Picky Oriesta Ayu Harmayanda Pratiwi Trisunuwati Prihatiningsih Pupimadita Tizar Afdora Putri, Fitria Tridyana Ramadhani, Sulistya Ika Randa, Sangle Yohanes Ratih Eka Dewayani Reo Radius Falah Reynaldy Hadi Ardyansyah Rica Silvi Anastasia Rizki Nur Azizah Rizki Prafitri Romy Abdillah Ramadhan Roro Rianthie Jessieca A.G Roselin Gultom Rositawati Indrati Ruli Nanda Pratama S. Chuzaemi S. Sulistyaningsih Sabarta sembiring Sangle Yohanes Randa Sari, Sri Ratna septiani anggitasari sitompul Siska OktaFina Diarlin Siska Tirajoh Siska Tirajoh, Siska Siti Nur Ulpah Sri Minarti Sri Ratna Sari Suyadi Suyadi Syahputra Wibowo Tasya Nur Karina Tika Septi Wardani Tri Ardiyati Tri Ardiyati Tri Ardyati Tri Ardyati Tri Eko Susilorini Ubaidillah, Muhammad Zaki Umi Kalsum Umiani Hatta USMAN ALI VM Ani Nurgiartiningsih Wahidin Mardiono Swantho Menggi Emma Widiati, Aris Sri Woro Busono, Woro Y.F Nuningtyas Yuli Frita Nuningtyas Yusuf, Zaen