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All Journal International Journal of Public Health Science (IJPHS) Jurnal Pendidikan IPA Indonesia Indonesian Journal of Mathematics and Natural Sciences Abdimas Forum Ilmu Sosial INTEGRALISTIK Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Sainteknol : Jurnal Sains dan Teknologi Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Bioeksperimen: Jurnal Penelitian Biologi Phenomenon : Jurnal Pendidikan MIPA BIOMA Unnes Political Science Journal Unnes Civic Education Journal Journal of Biology Education Unnes Science Education Journal Journal of Government and Civil Society Dinamisia: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Politik Indonesia: Indonesian Political Science Review Journal of Innovative Science Education AMALIAH: JURNAL PENGABDIAN KEPADA MASYARAKAT JURNAL BUDIDAYA PERTANIAN Jurnal ABDINUS : Jurnal Pengabdian Nusantara Abdimasku : Jurnal Pengabdian Masyarakat Jurnal Litbang Kota Pekalongan Life Science Dinamika Kesehatan: Jurnal Kebidanan dan Keperawatan Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Innovative: Journal Of Social Science Research Kartika: Jurnal Studi Keislaman Prosiding Seminar Nasional Biologi Prosiding Temu Ilmiah Nasional Guru Biosaintifika: Journal of Biology & Biology Education Unnes Civic Education Journal Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) Jurnal Info Kesehatan
Martien Herna Susanti
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Kinerja Dinas Pekerjaan Umum Dan Tata Ruang Kota Salatiga Dalam Pengembangan Tata Ruang Wilayah Tahun 2016 (Studi Kasus Taman Tingkir Kota Salatiga) Nahar, Akhmad Lutfi; Munandar, Moh. Aris; Susanti, Martien Herna
Unnes Political Science Journal Vol 1 No 2 (2017): July
Publisher : Program Studi Ilmu Politik Fakultas Ilmu Sosial Universitas Negeri Semarang

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Salatiga city is currently facing the influence over the function expansion of land where affected by the development of spatial locality. Public works-human settlements and Spatial planning Salatiga city as one of the Government agencies involved in standardization of spatial one public place to ensure the balance of the ecosystem of the city. Tingkir Park as one of place who became a city park and the activities of the citizens of most crowded at this time. Where is the year 2011-2016 public place in Salatiga city is just 15.9% meet the standard of 30%. Required performance improvements and spatial planning which can accommodate all kinds of activities to support the scope better. The issues examined in this study are (1) How the implementation of a performance carried out by the Department of public works and town of Salatiga in Spatial Development Tingkir Park in Salatiga city? (2) what factors affect the performance conducted Public works-human settlements and Spatial planning in Salatiga city spatial development in the region of the year 2016 (Case study on Tingkir Park, Salatiga)? This research in a research combination (mixed methods) with a model sequential exploratory. Research locations in the public works and of the city spatial salatiga and Tingkir Park. A kind of method research with qualitative and quantitative  approaches with the methods discriptive. The focus of this study is to understand  the implementation of the and the factors influences the performance of Public works-human settlements and Spatial planning in Salatiga pertaining with Tingkir park. The population and sample obtained using formulas Frank Lynch. Data collection in this research using interviews, the questionnaire, observation, documentation. The result of this research is the implementation of the performance of Public works-human settlements and Spatial planning in Salatiga in case study  Tingkir park  less fulfill standard green open space in Salatiga city with the central government 15.9 % of standard 30 %. But based on the research done through prespektif the community 60,3 % totally agree with an average 80,0 % so people really agree and satisfied with the Tingkir park  in salatiga city. After the Tingkir park, management less well until facilities damaged. As: game children and tiles. Factors that influences performance management department of Public works-human settlements and Spatial planning in Salatiga city seen from the individual, a personal capacity, and factors motivation. Where third this factor in public works agency and spatial planning prefer the ability and education. Employees to will be exposed through expression attitudes and the act of employees can perform the task and functions well. Advice for Salatiga city government should always involve the community in each phase because communities understand more what they want related policies implemented. And always optimize the implementation of performance management until progress of development of facilities and infrastructure that unkempt and efficiently beeing good. Salatiga Goverment must always support and provide development of facilities and infrastructure like city parks that  not only stop on building the park stage but until the maintenance and supervision to ensure the maintenance functions with good infrastructure.
Peran Kantor Pertanahan dalam Penyelesaian Sengketa Tanah antara Masyarakat Desa Pakis dengan Perhutani Kabupaten Kendal Saputro, Doni; Setiajid, Setiajid; Susanti, Martien Herna
Unnes Political Science Journal Vol 1 No 2 (2017): July
Publisher : Program Studi Ilmu Politik Fakultas Ilmu Sosial Universitas Negeri Semarang

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Land is included into one of human need which is functioning during the life and dead of the human himself. On one hand, the development of the era increases the needs of land. On the other hand, the land is unrenewable. It comes from the limitation of land-availability factors cannot afford the human breeding increase. Therefore, there is a big possibility of getting land-lawsuit among people and the officials. It can be seen from what happens in  Pakis village with the Forestry department of Kendal. Both of them are claiming the right of land owmership so that it needs mediation for the problem solving. This study is aimed at knowing the influence of Landlordy-department as the mediator and knowing the obstacles of this case. This study uses qualitative study by doing interviews to the informants related to this land-lawsuit case. The writer found that Kendal have passed through two mediation session and no clear result so the Land Office conduct the caucus as an alternative dispute settlement land by the people Village Pakis with Perhutani Kendal. The main closure  is that Forestry Department prosecutes the disputed-land in Pakis Village legally owned by the department and certified by the Forestry Department.
Implementasi Kebijakan Pengelolaan Sumber Mata Air Di Desa Nyatnyono Kabupaten Semarang Ali Rizqi, Nur Kholish; Susanti, Martien Herna; Sumarno, Sumarno
Unnes Political Science Journal Vol 1 No 2 (2017): July
Publisher : Program Studi Ilmu Politik Fakultas Ilmu Sosial Universitas Negeri Semarang

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The source of water Nyatnyono are the areas that have the potential is large enough in order to improve the quality of revenue the Nyatnyono village. Perdes No. 2 Tahun 2015 as a product of the village Nyatnyono, to set up the management of the assets of the Nyatnyono village to be neat and have a clear legal basis. Management of the assets of the village Nyatnyono as Perdes No. 2 Tahun 2015 so as not to be misused by certain elements so that it will be useful for the village and surrounding communities in metting of the needs. The formulation of a problem in this study are: 1) how the implementation of policy managements of springs Nyatnyono village as Perdes No. 2 Tahun 2015 of the village assets. 2) the impact of Perdes No. 2 Tahun 2015 to improve the welfare of the Nyatnyono. The study with the qualitative. The research in the Nyatnyono village the district of semarang. The research  is the implementation of policy and policy impact of the managements of water resources in the Nyatnyono village. The method of collecting data in this study interviewing, documentation and observation. Methods of data analysis in research by using a step 1) data collection 2) the reduction data 3) the presentation of data 4) the withdrawal of the conclusion. The research result showed that: 1) implementation Perdes of the managements village assets. village chiefs as a full management assets of the Nyatnyono village, and human resources in the management of the necessary supervision. 2) a positive impact in terms of the management of water located in the Nyatnyono village is traders around spring Kalimah Toyyibah and improvement of access roads Nyatnyono. The negative, lack of control or supervision of human resources in the field, especially the water resulted in the revenue the village used by unscrupulous persons who are not responsible for personal again. Advice: there needs to be given to Perdes No. 2 Tahun 2015 of the village assetsto clarify the implementation of the management asset as well the rural need for awareness of the government and society on the management of water resources in the Nyatnyono village to in policy process because conflict and the impact of the policy can benefit the Nyatnyono village. 2) socialization between the government of the village by society about implementation of Perdes. In addition the government Nyatnyono village in the implementation of the policy should also be maximal in terms of supervision agency execution in the field.
Peran Pemerintah Daerah Kota Bekasi Dalam Mewujudkan Penataan Ruang Terbuka Hijau Cahyo, Reza Dwi; Susanti, Martien Herna; Sunarto, Sunarto
Unnes Political Science Journal Vol 1 No 2 (2017): July
Publisher : Program Studi Ilmu Politik Fakultas Ilmu Sosial Universitas Negeri Semarang

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The city of Bekasi, including one of the city are hard to do development in all areas, including the revamping of the city. The problem of green open space in Bekasi of particular, require the handling of the structurally through various studies and policy given the green open a remote ecosystem a particular environment for the area being developed. There needs to be the role of local goverments in realizing the arrangement of green open space in Bekasi City. The formulation of the problem of this study is. 1) If the efforts made by the city of Bekasi in the realization of green open space?, 2) How public participation in the realization of green open space in the city of Bekasi?, 3) Factors are to be an obstacle to the local government in pursuit of green open space in the city of Bekasi? The study with the qualitative. The research at the center of the city of Bekasi and the public city of Bekasi. An informant for the research is staff BAPPEDA the city of Bekasi, staff official city planning of Bekasi city and society. Focus of research which is 1) Local goverments, 2) Public participation, 3) Factors in the opposite direction. Techniques of collecting data in an observation, interviews, and documentation. Technical analysis includes data collection, the reduction of data, the presentation of data, and the withdrawal of the conlusion. The results showed that: 1) The city of Bekasi set the location decided by the mayor of Bekasi through the regulations of the mayor of Bekasi number 5 year 2015 of the way the provision of incentives and disincentives in the control of the utilization of space in the city of Bekasi, local government provides incentives to people who are encouraged to do the use of green open space in the city of Bekasi. 2) Forms of public participation submitted to the local government city of Bekasi in oral or written and delivered to the department of related with counseling and guidance of green open space, a campaign of green open space through the mass media and electronics, making the green school and green campus, the fulfillment of the open green space, community organizations and the award to the public has a green open space. 3) Public awareness in the provision of green open space, there are still obstacles both obstacles the internal and external constraints, obstacles internally by the constraints the government and external problems is the constraint of the community people. Advice : 1) There needs to be the program and activites that are more about green open space itself as weel as socialize the program has been made so that people can understand a program for local governments, 2) For the people there should be a concern about the green open space to the creation of a comfortable, environment and clean, 3) The city of Bekasi to disseminate green open space to the community by providing knowledge about the importance of achieving green open space through regional regulation in order to create awareness of people that can preserve and maintain green open space in the city of Bekasi
KOMUNIKASI POLITIK PARTAI KEBANGKITAN BANGSA DENGAN MASYARAKAT BERBASIS NU DI KECAMATAN KESUGIHAN KABUPATEN CILACAP INDIARTO, ARIF; Sunarto, Sunarto; Susanti, Martien Herna
Unnes Civic Education Journal Vol 2 No 2 (2016)
Publisher : Civic Education Program, Universitas Negeri Semarang

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Komunikasi politik merupakan suatu proses penyampaian  informasi oleh pimpinan partai politik terhadap masyarakat berbasis NU dengan media berupa lisan maupun tulisan dan diharapkan anggota partai politik tersebut mengerti dan menyetujui dari apa yang telah disampaikan. Partai politik yang mendapat dukungan dari masyarakat berbasis NU di Kecamatan Kesugihan adalah PKB. Salah satu basis PKB adalah NU. Dari sekian banyaknya masyarakat  NU, di Kecamatan Kesugihan merupakan salah satu basisnya. Permasalahan penelitian ini adalah: bagaimanakah komunikasi politik PKB dengan masyarakat berbasis NU di Kecamatan Kesugihan? faktor-faktor penghambat terjadinya komunikasi politik Partai PKB dengan masyarakat berbasis NU di Kecamatan Kesugihan,penelitian ini menggunakan pendekatan kualitatif. Lokasi penelitian ini di Kecamatan Kesugihan. Teknik pengumpulan data dengan wawancara, observasi dan dokumentasi. Peneliti menggunakan teknik triangulasi. Hasil penelitian DPAC PKB Kecamatan Kesugihan melakukan komunikasi politik dilakukan dengan cara sederhana, lewat pengajian, yasinan, minggu kliwonan. Faktor penghambat komunikasi politik PKB dan masyarakat berbasis NU yaitu terlalu luasnya Kecamatan Kesugihan, Saran dalam penelitian ini: kepada PKB, senantiasa meningkatkan perannya dalam berkomuikasi politik, perlu meningkatkan komunikasi dan melakukan pendekatan kepada masyarakat.Political communication is a process of delivering information by leaders of political parties based on the society in the form NU with verbal and written media’s and political party members are expected to understand and approve of what has been delivered. The political party that has the support of the community-based NU in Kesugihan’s Subdistrict is PKB. PKB is one of the bases NU. Among the many NU society, in the Kesugihan’s Subdistrict is one base. The problemin this research is: how political communication agreements with community-based NU in Kesugihan Subdistrict? inhibiting factors the PKB’s political communication with the community-based NU in Kesugihan subdistrict, this research used a qualitative approach. This research sites in Kesugihan Subdistrict. Techniques of data collection by interview, observation and documentation. Researcher use triangulation techniques. The results DPAC PKB’s Kesugihan Subdistrict in political communication is done in a simple way, through lectures, yasinan, Kliwonan. Inhibiting factors PKB political communication and NU based society is too breadth Kesugihan Subdistrict, Suggestions in this research: to PKB, communicated constantly role increasing in politics, need to improve communication and approach to society
KAMPANYE KADER PEREMPUAN PARTAI GOLONGAN KARYA DALAM PEMILU LEGISLATIF TAHUN 2009 DI KUDUS FERDIANA, RAHMA; Susanti, Martien Herna; Lestari, Puji
Unnes Civic Education Journal Vol 2 No 2 (2016)
Publisher : Civic Education Program, Universitas Negeri Semarang

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Pada pemilu 2009 Partai Golkar salah satu partai yang sukses, dengan terpilihnya 6 anggota DPRD di Kabupaten Kudus dengan komposisi 4 kursi kader laki-laki dan 2 kursi kader perempuan. Terpilihnya 2 kader perempuan Partai Golkar dalam pemilu legislatif tahun 2009 tidak terlepas dari adanya komunikasi pada saat kampanye. Permasalahan dalam penelitian ini mencakup: (1) Bagaimana kampanye kader perempuan Partai Golongan Karya dalam Pemilu Legislatif tahun 2009 di Kabupaten Kudus?, (2) Apakah faktor-faktor yang mendukung dan menghambat kampanye kader perempuan Partai Golongan Karya dalam Pemilu Legislatif tahun 2009 di Kabupaten Kudus?. Hasil penelitian menunjukan kampanye yang dilakukan oleh kader perempuan Partai Golkar dalam pemilu legislatif tahun 2009 di Kabupaten Kudus sudah menggunakan kerangka konsep atau langkah-langkah dalam kampanye, dimulai dari merumuskan tema, proses perencanaan mengorganisasi kampanye,penganggaran kampanye, pelaksanaan kampanye dan evaluasi kampanye. Faktor yang mendukung dan menghambat kampanye kader perempuan Partai Golongan Karya terdiri dari faktor internal, dan eksternal. Faktor internal yang mendukung adalah Image Partai Golkar, keluarga, dan organisasi. Faktor internal yang menghambat adalah sumber dana. Faktor eksternal yang mendukung adalah media sedangkan faktor eksternal yang menghambat adalah waktu dan tempat, serta sikap pragmatisIn the 2009 election Golkar party a successful one , with the election of six members of parliament in the Holy District 4 seat cadre composition of men and 2 women cadres seat . 2 women elected Golkar Party cadres in the legislative elections of 2009 can not be separated from the lack of communication during the campaign . Problems in this study include : ( 1 ) How women cadres campaign Golkar Party in the legislative election of 2009 in Kudus Regency ? , ( 2 ) What are the factors that support and hinder women cadres campaign Golkar Party in the legislative election of 2009 in the District Holy ? . The results showed that the campaign conducted by the Golkar Party cadres women in the 2009 legislative elections in the Holy District already uses the conceptual framework or the steps in the campaign , starting from formulating themes , organized campaign planning process , budgeting, campaign , campaign execution and campaign evaluation . Factors that support and hinder women's campaign Golkar Party cadres composed of internal factors and external . Internal factors that support is Golkar Party Image , families , and organizations . Internal factors that inhibit the source of funds . External factors that support is medium while the external factors that hamper is a time and place , as well as a pragmatic attitude
KEHIDUPAN SOSIAL EKONOMI PEREMPUAN DALAM MASYARAKAT NELAYAN DI DESA PANJANG BARU KECAMATAN PEKALONGAN UTARA MASITHO, BAHRAIN DWI; Lestari, Puji; Susanti, Martien Herna
Unnes Civic Education Journal Vol 2 No 2 (2016)
Publisher : Civic Education Program, Universitas Negeri Semarang

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Kemiskinan yang terjadi pada masyarakat nelayan membuat seluruh anggota keluarga pada masyarakat nelayan ikut ambil andil dalam mencari nafkah untuk memenuhi kebutuhan sehari-hari, terutama perempuan nelayan. Keterlibatan perempuan nelayan dalam mencari nafkah, tidak diimbangi dengan kemampuan dan ketrampilan yang dimiliki sehingga perlu adanya pemberdayaan untuk masyarakat nelayan, teritama untuk perempuan nelayan agar mereka dapat meningkatkan kualitas hidup mereka. Penelitian ini ditujukan untuk mengetahui kehidupan sosial ekonomi perempuan dalam masyarakat nelayan di Desa Panjang Baru Kecamatan Pekalongan Utara dan untuk mengetahui kualitas hidup perempuan dalam masyarakat nelayan di Desa Panjang Baru Kecamatan Pekalongan Utara. Penelitian ini menggunakan metode penelitian kualitatif dengan teknik triangulasi sumber. Hasil penelitian ini adalah kehidupan sosial ekonomi perempuan nelayan sebagai ibu rumah tangga, akan menjalankan perannya sebagai ibu rumah tangga dengan baik karena mereka dapat mengurus pekerjaan rumah dengan baik. Sedangkan kehidupan sosial ekonomi perempuan nelayan yang bekerja, mereka harus bisa membagi waktu antara mengurus pekerjaan rumah dengan bekerja sehingga mereka memiliki beban ganda dalam kehidupannya. Kualitas hidup perempuan dalam masyarakat nelayan di Desa Panjang Baru Kecamatan Pekalongan Utara dikatakan kurang baik karena rendanya kualitas sumber daya manusia sebagai akibat keterbatasan akses sekolah dan kesehatan, pemukiman yang kurang layak, dan kurangnya sarana dan prasana seperti sekolah, pasar dan kamar mandi umum.Poverty occurs in fishing communities makes the whole family on fishing communities took part in making a living to meet daily needs, especially women fishers. The involvement of women in fishing for a living, is not matched by the ability and the skills so necessary for the empowerment of fishing communities, women teritama for fishermen so they can improve their quality of life. This study aimed to determine the social-economic life of women in fishing communities in Panjang Baru village of Northern District of Pekalongan and to determine the quality of life of women in fishing communities in Panjang Baru village of Northern District of Pekalongan. This study uses qualitative research methods with a triangulation technique. The results of this research are social and economic life as a fishing woman housewife, will perform its role as a housewife with either because they can take care of the house work well. While the women's social and economic life of fishermen who work, they should be able to divide their time between taking care of homework to work so that they have a double burden in life. Quality of life of women in fishing communities in the village of North Pekalongan District of New Long dikatan unfavorable because the lace quality of human resources as a result of limited access to schools and health care, housing less viable, and the lack of facilities and infrastructures such as schools, markets and public bathrooms.
EFEKTIVITAS PENERAPAN METODE PERMAINAN HAPPY KINGDOM PADA MATERI KEANEKARAGAMAN MAKHLUK HIDUP TERHADAP AKTIVITAS DAN HASIL BELAJAR SISWA SMP Kinayungan, Irna; Susanti, R; Chasnah, Chasnah
Journal of Biology Education Vol 5 No 3 (2016)
Publisher : FMIPA UNNES

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Penelitian ini bertujuan untuk mengukur keefektifan penerapan metode permainan happy kingdom pada materi keanekaragaman makhluk hidup terhadap aktivitas dan hasil belajar siswa kelas VII. Metode penelitian yang digunakan pra-experimental dengan desain one-shot case study. Populasi dalam penelitian ini adalah seluruh siswa kelas VII MTs NU Ungaran tahun ajaran 2014/2015 dengan sampel siswa kelas VII D dan VII E yang diambil menggunakan teknik cluster random sampling. Prosedur penelitian meliputi tahap persiapan (observasi, penyusunan perangkat pembelajaran dan instrumen tes) dan pelaksanaan (pembelajaran, penilaian dan evaluasi). Data aktivitas siswa diambil melalui observasi, data hasil belajar melalui metode tes, sedangkan tanggapan siswa dan guru diambil melalui angket. Hasil penelitian menunjukkan persentase keaktifan siswa sebesar 75,51% dengan ketuntasan siswa mencapai 82,43%, menandakan bahwa penelitian ini efektif karena >80% siswa memperoleh nilai ≥ KKM 70. Persentase tanggapan guru sebesar 86% (dikategorikan sangat baik) dan sebanyak 85% siswa menyatakan setuju terhadap metode permainan happy kingdom menandakan bahwa siswa sangat tertarik. Kesimpulan dari penelitian ini adalah penerapan metode permainan happy kingdom efektif diterapkan pada materi keanekaragaman makhluk hidup kelas VII terhadap aktivitas dan hasil belajar siswa. This study aims to determine the effectiveness application of happy kingdom game method in grade VII diversity of living things learning. The research method of pre-experimental design is one-shot case study. The population in this study were all students of grade VII MTs NU Ungaran year 2014/2015, and sample students of class VII D and VII E were taken using cluster random sampling technique. Research procedure includes preparation phase (observation, preparation of learning tools and test instruments) and implementation phase (learning, assessment and evaluation). Data retrieved through observation of student activity, learning outcomes data through test method, while students and teachers responses were taken through a questionnaire. Results of study include the percentage of student activity amounted to 75.51%, and the completeness of students reached 82.43%, indicating that learning process is effective for> 80% of students received grades KKM ≥ 70. Percentage of 86% teacher responses were categorized as excellent and as much as 85% of students very interested to the game happy method kingdom. The conclusion from this study is application of game happy kingdom method effectively applied to diversity of living things learning at grade VII science classroom.
Pengaruh Pemberian Vitamin A terhadap Penurunan Parasitemia Mencit Strain Swiss yang diinfeksi Plasmodium berghei Isnaeni, Umi; Iswari, Retno Sri; WH, Nugrahaningsih; Susanti, R.
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.3930

Abstract

Vitamin A merupakan vitamin yang berperan sebagai imunostimulan. Penelitian ini bertujuan untuk mengetahui apakah pemberian vitamin A dapat menurunkan parasitemia Plasmodium berghei pada mencit strain Swiss. Penelitian ini menggunakan 24 ekor mencit strain Swiss jantan berumur 6-8 minggu dengan berat badan 20-30 gram/ekor. Penelitian dilaksanakan secara eksperimental dengan menggunakan time series design. Dalam penelitian ini dilakukan perlakuan berupa pemberian vitamin A dengan 3 variasi dosis yaitu 0 IU/g BB, 35 IU/g BB dan 70 IU/g BB serta kelompok kontrol negatif dengan masing- masing kelompok terdiri dari 6 ekor mencit. Vitamin A diberikan 1 jam sebelum penginfeksian dan mencit dirawat sampai mencit pada kelompok kontrol negatif mati. Sediaan apus darah tipis dibuat 2 hari sekali dan parasitemia dihitung dengan pengecatan Giemsa. Data parasitemia dianalisis dengan ANOVA.Untuk hasil yang signifikan maka dilanjutkan dengan uji lanjut Post hoc pada taraf kesalahan 1%. Hasil uji ANOVA untuk kelompok perlakuan B, C dan D diperoleh nilai p <0,001 pada masing-masing kelompok perlakuan. Hal tersebut menyatakan bahwa adanya perbedaan yang signifikan pada perlakuan yang diberikan. Begitu juga untuk uji lanjut Post hoc yang telah dilakukan diperoleh nilai p < 0,001. Dapat disimpulkan bahwa pemberian vitamin A berpengaruh terhadap penurunan parasitemia Plasmodium berghei pada mencit strain Swiss.Vitamin A is a vitamin that acts as an immunostimulant. This research aims to determine whether the administration of vitamin A can reduce parasitaemia of Plasmodium berghei in Switzerland strain mice. This research used 24 mice Switzerland strain mice 6-8 weeks old weighing 20-30 grams/tail. Research conducted experimentally by using time series design. In this research, the provision of vitamin A treatment with 3 doses of variation is 0 IU/g BW, 35 IU/g BW and 70 IU/g BW as well as the negative control group, with each group consisting of 6 mice. Vitamin A was given 1 hour before infection then treated mice to mice in the negative control group died. Thin blood smears were made 2 days and parasitaemia was calculated by Giemsa staining. Parasitaemia data were analyzed with ANOVA. Significant results then followed by a further test Post hoc at 1% error level. ANOVA test results for the treatment group B, C and D obtained value of p <0.001 for each treatment group. It is claimed that there are significant differences in the treatment given. Likewise for further Post hoc testing that has been done obtained value of p <0.001. It can be concluded that the administration of vitamin A affects the reduction in parasitaemia of Plasmodium berghei in Switzerland strain mice.
ISOLASI DAN IDENTIFIKASI VIRUS AVIAN INFLUENZA SUBTIPE H5N1 DI PETERNAKAN TRADISIONAL KECAMATAN GUNUNGPATI SEMARANG Wibowo, Angga Ari; Susanti, R.; Ulum, Farikhul; W.H., Nugrahaningsih
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2270

Abstract

Avian Influenza (AI) atau yang lebih dikenal dengan flu burung disebabkan oleh virus influenza yang bermutasi menjadi patogen. Penelitian tentang isolasi dan identifikasi virus AI subtipe H5N1 perlu dilakukan untuk mengetahui keberadaan virus tersebut khususnya di kecamatan Gunungpati. Desain penelitian adalah eks ploratif dengan pengumpulan sampel usap kloaka secara acak di lima kelurahan di kecamatan Gunungpati. Sampel usap kloaka ditumbuhkan pada telur ayam berembrio SPF, kemudian diisolasi RNA-nya dilanjutkan dengan identifikasi subtipe virus AI menggunakan Reverse Transcriptase-Polymerase Chain Reaction (RTPCR) dengan primer pendeteksi gen H5 dan N1. Hasil positif apabila visualisasi hasil elektroforesis dari produk PCR menunjukkan pita-pita spesifik panjang 219 bp untuk H5 dan 131 bp untuk gen N1-nya. Limapuluh sampel usap kloaka yang diisolasi dari lima kelurahan di Gunungpati, delapan isolat positif VAI dan enam diantaranya positif H5N1 dengan angka prevalensi 12%. Isolat positif berasal dari 2 spesies itik (16,67%), 2 dari entok (11,76%) dan 2 dari angsa (18,18%). Dari lima kelurahan yang diambil sampelnya, tiga kelurahan ditemukan positif virus H5N1 masing-masing kelurahan Sekaran (6,67%), Kalisegoro (16,67%) dan Pakintelan (15,78%). Unggas-unggas air di peternakan unggas tradisional berpotensi sebagai penularan virus AI, khususnya subtipe H5N1.Avian Influenza (AI) or better known as bird flu is caused by influenza viruses that mutate into a pathogen. Research on the isolation and the identification of H5N1 subtype needed to be carried out to determine the presence of the virus, particularly in the subdistrict of Gunungpati. The study design was explorative by collecting cloacal swab samples randomly from five villages in Gunungpati. The cloacal swab samples were cultured in embryonated SPF chicken eggs, then the RNA was isolated and followed by the identification of AI virus subtype using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) with H5 and N1 gene detecting primers. A positive result is obtained if the visualization of the electrophoresis of PCR products showed bands with specific length of 219 bp for H5 gene and 131 bp for N1 gene. Fifty cloacal swab samples were isolated from five villages in Gunungpati, and eight of them were positive isolates and six of them were H5N1 positive with the prevalence rate of 12%. The positive isolates were derived from two species of duck (16,67%), 2 from wild duck (11,76%) and 2 from geese (18,18%). Of the five sampled villages, three villages were found to be H5N1 positive, i.e. Sekaran village (6,67%), Kalisegoro village (16,67%) and Pakintelan village (15,78%). Water birds in traditional poultry farming were considered potential as the transmitters of AI virus, particularly of H5N1 subtype.
Co-Authors - Suprapti, - A Yuniastuti A.A. Ketut Agung Cahyawan W Adhi, Nino Aditya Marianti Aji Purwinarko, Aji Ali Rizqi, Nur Kholish Ali Rizqi, Nur Kholish Ana Fitria Ananda, Ananda Anandha Anandha, Anandha Andi Suhardiyanto Andreas Priyono Budi Prasetyo Angga Ari Wibowo Ani Rusilowati Ari Yuniastuti Ariesta, Putri Ariesta, Putri ARIF INDIARTO, ARIF Arlinda, Dyken Dwi Asri, Widi Aulia, Innarotul Aulia, Maliqa Azkiyah, Nimas Catur BAHRAIN DWI MASITHO, BAHRAIN DWI Bahtiar, Denny Herbianto Cahya Buana Cahyati, Widya Hari Cahyo, Reza Dwi Cahyo, Reza Dwi Chasnah Chasnah, Chasnah Chatarina Umbul Wahyuni Christijanti, W. Dafip, M. Dewi Mustikaningtyas Dewi, Nuriana Rahmani DIAN LESTARI Dimarti, Safira Chaerani Dimarti, Safira Chairani Dita Selviana, Dita Doni Saputro E Hidayat, E E.S.,, Sri Mulyani Endah Peniati ENDANG TRI MARGAWATI Enni Suwarsi Rahayu F Widhi Mahatmanti Fachry Abda El Rahman Falistina, Amna Farikhul Ulum Felintina Yuniarti, Felintina Fidia Fibriana Fifti Istiklaili, Fifti Firdausi, Aulya Della Fitri Arum Sasi, Fitri Arum Fitriya, Farikhatun Nur Gita Ria Octafia, Gita Ria Hammam, Bagus Hardi Saputro, Iwan Hasani, Azharu Alfi I Gusti Ngurah K Mahardika I Gusti Ngurah Kade Mahardika I Wayan T Wibawan I-W.T Wibawan Ibnul Mubarok Indriawati, Indriawati Irna Kinayungan, Irna Isnaeni, Lili Astuti Isnaeni, Lili Astuti Isti Hidayah Iswari, R S Jaya, Adi Franata Kenarni, Naina Rizki Kristamtini, K Kusuma, Fitriana Dian Kusuma, Putri Patria Larasanti, Firna Larasanti, Firna Lina Herlina Listiaji, Prasetyo M.T Suhartono Maggy T Suhartono Mahardika Prasetya Aji Maman Rachman Margareta Rahayuningsih Nugroho Edi K Moh. Aris Munandar, Moh. Aris Mohamed, Mohd Salim Bin Mukaromah, Riska Laila Muyasaroh, Kun Maisarotun Na’ima, Mirtaati Nahar, Akhmad Lutfi Nahar, Akhmad Lutfi Naim, Fatchun Nezar, Muhammad Rofiq Ning Setiati Nisa, Farah Fitrotun Nissa, Ma’isyatun Nugrahaningsih Nugrahaningsih Nugrahaningsih WH, Nugrahaningsih Nur Halimah Nur Kusuma Dewi Nur Rahayu Utami Oktaviani, Esa Pauline, Maretha Putri Pramesti Dewi Pratiwi, Retno Herningrum Priyantini Widiyaningrum PUJI LESTARI Pukan, Krispinus Putri, Amalia Nurina Putri, Komala Amelia R, Rahmatika Saputri R.D Soejoedono RAHMA FERDIANA, RAHMA Rahmatih, Aisa Nikmah Rahmatih, Aisa Nikmah Rahmawati, Esa Mara Rahmawati, Sinta Kurnia Rakainsa, Senda Kartika Ratih Puspita Dewi, Ratih Puspita Retna Utami Retno D Soejoedono Retno Sri Iswari Rizki Puspita Dewi, Rizki Puspita Rohmah, Amalia Nor Romadhoni, Mar`atush Sholihah RS Iswari S.Pd. M Kes I Ketut Sudiana . Sa’adah, Siti Rofiatus Salfia, Mega Salwa, Khoerina Samsul Nizar Saputri, Aprilia Rizki Saraswati, Ifah Saraswati, Ifah Septiana Anggraini, Septiana Setiadi, Eka Setiajid Setiajid Setiajid Setiajid, Setiajid Shinta Widyastuti, Shinta Sigit Saptono Siti Harnina Bintari Sri Sukaesih Sri Utami Sriyadi Sriyadi, Sriyadi Subagyo Subagyo Sulistyo, Danu Sumarno . Sunarto Sunarto Supriyanto Supriyanto Suryo Pratikwo Suryonegoro, Bayu Murti Sutikno Sutikno Tafrika Donita Talitha Widiatningrum Tijan Tijan, Tijan Tri Budi Kurniawan, Tri Budi Trisna, Mila Tuti Widianti Umi Isnaeni, Umi Utami, Anisa Dyah Utami, Melia Dwi Utomo, Didik Huswo Wahyuni, Desiy Ema Dwi Wardono, Hari Rudijanto Indro Widya Hary Cahyati Widyaningrum, Kartika Wijaya, Ifan Badra Wijayanti , Yuni Wiwi Isnaeni Wulan Christijanti Wulandari K. E, Yenni Tyas Yohanes Nong Bunga Yuliana, Lusia Retno Yuliana, Lusia Retno Yuliantika, Atika Yuni Wijayanti Yuniastuti , Ari Yuniastuti, A. Yustinus Ulung Anggraito Zaenafree, Intan Zakyani, Nova N