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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Media Kesehatan Masyarakat Indonesia JURNAL GIZI INDONESIA Journal of the Medical Sciences (Berkala Ilmu Kedokteran) El-Hayah : Jurnal Biologi Jurnal Kesehatan Masyarakat Indonesian Journal of Cancer Chemoprevention Journal of Biomedicine and Translational Research The Indonesian Biomedical Journal Universa Medicina Biosaintifika: Journal of Biology & Biology Education YARSI Medical Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research Medica Hospitalia Medica Arteriana (Med-Art) Journal of Telenursing (JOTING) Diponegoro International Medical Journal (DIMJ) Narra J Bioscientia Medicina : Journal of Biomedicine and Translational Research Life Science Jurnal Locus Penelitian dan Pengabdian JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Indonesian Journal of Biomedicine and Clinical Sciences Folia Medica Indonesiana
Neni Susilaningsih
Anatomy Histology Department, Faculty Of Medicine, Diponegoro University, Semarang, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health

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Histopathological changes in pregnant mice's hepar and kidney following exposure to kelor (Moringa oleifera) leaf extract Armalina, Desy; Susilaningsih, Neni; Saraswati, Indah
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 13, No 2 (2025): June
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jgi.13.2.145-151

Abstract

Background: Moringa provides extra dietary benefits. The immune system is significantly influenced by the nine necessary amino acids, calcium, iron, potassium, magnesium, zinc, and vitamins A, C, E, and B that are included in this food. Previous studies state that moringa causes liver and kidney damage. Another study found that moringa has an abortive effect.Objectives: This study aimed to determine how exposure to kelor (moringa oleifera) leaf extract affected the hepar and kidney in pregnant mice and found the secondary metabolite of the moringa leaves.Materials and Methods: An experimental laboratory design was conducted in 24 pregnant female Balb/c mice. They were randomized into four groups. Group K was not given anything. Group P1-P3 were given moringa leaf extract during pregnancy at days with a dose dose of 10, 20, and 30 mg in 10, 20, and 18 days, and termination was done to take the organs and make the tissue processing.Results: We found no dead mice and no aggression during the experiment. Significant statistics differed between the experimental group in the microscopic appearance of the kidney and hepar. This study found degeneration and inflammation in the hepar and kidney in the treatment group. Qualitative phytochemical tests on leaves contained alkaloids, flavonoids, phenolics, tannins, and steroids.Conclusion: The ethanol extract of Moringa leaf has shown a mild heparin and kidney effect compared to the control.
Effectiveness of Andrographis paniculata Extract Nanoparticle on The Expression of CD4+ and CD8+ of Rats Listeriosis Iffiyana, Alifatul; Farida, Helmia; Susilaningsih, Neni
El-Hayah:Jurnal Biologi Vol 10, No 2 (2025): EL-HAYAH (VOL 10, NO 2 Maret 2025)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v10i2.32207

Abstract

Listeria monocytogenes is a Gram-positive bacterium that causes listeriosis known as foodborne disease. L. monocytogenes infection will activate both innate and adaptive immune responses. Androghrapis paniculata has an effect as immunostimulant. Nanocapsulation-chitosan can increase solubility and reduce the particle size making it easier for compounds to enter the cell. This research focuses on the utilization of nanoparticle A. paniculata as an immunomodulator against CD4+ and CD8+. Study design was an experimental study with post only control group trial. A total of 30 male Wistar rats divided into 6 groups, with 5 rats in each group. All groups were injected by L. monocytogenes intravenous except Normal Group. EAP 200 group was administered oral crude extract of A. paniculata at dose 200 mg/kg BW for 7 days. nEAP 100, nEAP 200 and nEAP 400 groups were administered oral Nanoparticle-Extract of A. paniculata at dose of 100 mg/kg BW, 200 mg/kg BW, and 400 mg/kg BW respectively for 7 days. CD4+ and CD8+ cells were detected by isolating PBMC cells then analyzed using flow cytometry. Statistical analysis applied ANOVA. Both crude extract of EAP 200 and nanoparticles nEAP 400 significantly increase the number of CD4+ (P= 0.050) and CD8+ (P= 0.010). Crude and nanoparticles extract of A. paniculata increase the production of CD4+ and CD8+ cells in rats infected with L. monocytogenes.
Mapping the Research Landscape of Natural Compounds for Tuberculosis Treatment: A Bibliometric Approach Hakam, Abdul; Susilaningsih, Neni; Mexitalia, Maria; Susanto, Hardhono; SyarofilAnam, Moh.
Jurnal Locus Penelitian dan Pengabdian Vol. 4 No. 5 (2025): JURNAL LOCUS: Penelitian & Pengabdian
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/locus.v4i5.3981

Abstract

Tuberculosis (TB) remains a global health crisis, exacerbated by rising drug resistance in Mycobacterium tuberculosis. While conventional therapies like BPaL/BPaLM regimens and shorter rifapentine-based treatments show promise 11–33, the need for novel anti-TB agents persists. Natural compounds, particularly from biodiverse regions like Indonesia 77, offer untapped potential, yet prior bibliometric analyses lack comprehensive integration of computational and multi-omics approaches to guide future research. This study maps the research landscape of natural compounds for TB treatment (2015–2025) through bibliometric analysis, identifying gaps and proposing AI-driven, multi-disciplinary strategies to accelerate drug discovery. PubMed-derived data (23 articles) were analyzed using VOSviewer to visualize co-authorship, keyword co-occurrence, and thematic clusters. Trends in authorship, geographic contributions, and research foci (e.g., molecular docking, drug resistance) were evaluated. China dominated research output (11/23 studies), with clusters emphasizing computational methods (e.g., virtual screening), bacterial enzymes, and animal models. Keyword analysis revealed a strong focus on drug resistance and synergism, yet limited exploration of AI, multi-omics, or ethnopharmacology. Notably, studies like Romulo et al. (2018) highlighted Indonesian plants’ anti-TB potential 77, but systematic integration with modern technologies remains underexplored. This study identifies a critical gap: the need to merge traditional natural compound research with AI-aided drug design, multi-omics (e.g., transcriptomics 1616), and nanodelivery systems. By proposing a framework that bridges computational predictions (e.g., molecular docking 1515) with experimental validation, this research advances a novel, scalable approach to combat drug-resistant TB, leveraging global biodiversity and cutting-edge technologies.
Andrographis paniculata Leaves Extract Inhibit TNF-α and Caspase-3 Expression of Septic Rats’ Intestinal Tissues Ardika, Ryco Giftyan; Budiono, Bernardus Parish; Widiastiti, Nyoman Suci; Maharani, Nani; Susilaningsih, Neni; Sandra, Ferry
The Indonesian Biomedical Journal Vol 16, No 1 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i1.2727

Abstract

BACKGROUND: Microcirculation and cellular disturbances caused by sepsis might trigger significant intestinal damage. Andrographis paniculata extract decreases inflammatory intestinal epithelial cells with its role as an antiparasitic and anti-inflammatory agent. However, A. paniculata extract’s effect on sepsis have not been commonly studied, especially in the intestinal tissues. Therefore, this study was conducted to determine A. paniculata leaves extract (APLE) effect in sepsis-induced intestinal tissues of rats by examining the expression of inflammatory cytokines involved in sepsis, namely tumor necrosis factor (TNF)-α and Caspase-3.METHODS: Rats were divided into five groups; two groups received no pretreatment and the other three groups received 200, 400, and 500 mg/kg BW/day APLE, respectively. Three pretreated groups and one group with no pretreatment were then injected with 1 mg/200 g BW lipopolysaccharides (LPS) intraperitoneally to create septic rat models. Three days after the LPS-induction, rats were euthanized and the expression of TNF-α and Caspase-3 were assessed based on the immunohistochemical staining of rats’ intestinal tissues.RESULTS: Compared with NaCl (sham), LPS significantly (p<0.001) induced TNF-α expression from 6.60±1.36 to 25.37±1.74. Pretreatment of 200, 400, and 500 mg/kg BW/day APLE could significantly (p<0.001) inhibit the LPS-induced TNF-α expression (18.82±1.36, 11.45±1.18, and 6.89±1.90, respectively). Similar with TNF-α, compared with NaCl (sham), LPS significantly (p<0.001) induced Caspase-3 expression from 6.92±1.66 to 23.59±2.25. Pretreatment of 200, 400, and 500 mg/kg BW/day APLE could significantly (p<0.001) inhibit the LPS-induced Caspase-3 expression (17.47±1.68, 12.99±1.51, and 5.59±1.51, respectively).CONCLUSION: The pretreatment of APLE could inhibit the LPS-induced TNF-α and Caspase-3 expression, therefore APLE could be suggested as a potential sepsis-preventing agent.KEYWORDS: Andrographis paniculata, sepsis, TNF-α, Caspase-3, lipopolysaccharide 
Addition of Beetroot Extract to Neoadjuvant Adriamycin Cyclophosphamide Regimen Increased Tumor Cell Apoptosis in Mammary Adenocarcinoma Rats Susilowati, Sri; Susilaningsih, Neni; Suharti, Catharina
Indonesian Journal of Cancer Chemoprevention Vol 12, No 3 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss3pp130-136

Abstract

Apoptosis is one of the anticancer targets. Currently, the concomitant use of phytotherapy products and chemotherapy regimens is common in breast cancer patients. The purpose of this study was to examine the apoptotic effect of adding beetroot extract to the neoadjuvant Adriamycin Cyclophosphamide (AC) regimen by observing the expression levels of p53 and caspase 3 in tumor tissue from mammary adenocarcinoma rats. Twenty-four rats that succeeded in growing tumor nodules were randomly divided into 4 treatment groups: without treatment, AC only treatment, AC plus beetroot extract at dose of 25 and 100 mg/kg BW, respectively. AC was given 4 cycles in doses of 5 and 50 mg/kg body weight intraperitoneally every week. Tumor tissue was dissected at 4th week for examination of p53 and caspase 3 expression levels using the qRT-PCR method. The addition of beetroot extract at doses of 25 and 100 mg/kg BW in the neoadjuvant AC regimen showed significantly higher levels of p53 and caspase 3 expression than those with AC treatment alone. These results proved that beetroot extract has a synergistic effect with neoadjuvant AC regimen by increasing tumor cells apoptosis.Keywords: Beetroot extract, Adriamycin, Cyclophosphamide, apoptosis, p53.
Healing of Radiation Dermatitis with Ozonated Aloe Vera Oil by Increasing PDGF and Epidermal Thickness in Sprague-Dawley Rats Utami, Widoasti Putri; , Yan Wisnu Prajoko; Prihharsanti, Christina H.N.; Sadhana, Udadi; Susilaningsih, Neni; Restiwijaya, Maryam; Nur, Muhammad
Folia Medica Indonesiana Vol. 58, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: • This study demonstrated the effect of ozonated Aloe vera oil in the healing of radiation dermatitis wound. • Groups that received no treatment was compared with groups that received treatment using 2.5% hydrocortisone ointment and ozonated Aloe vera. • Ozonated Aloe vera oil improves the healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness. Abstract : The long-term use of corticosteroids as a standard treatment for skin disorders, such as radiation dermatitis, can cause many side effects. Alternatively, ozonated Aloe vera oil may replace corticosteroids due to its fewer side effects and benefits in wound healing process. Re-epithelialization and the formation of growth factors, such as platelet-derived growth factor (PDGF), play an important role in the healing of dermatitis wound. This study intended to demonstrate the effect of ozonated Aloe vera oil to improve the healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness. This study used a post-test only control group design. A sample of 36 Sprague-Dawley rats was divided into 6 groups (C1=without treatment, C2=2.5% hydrocortisone ointment, P1=pure Aloe vera (AV), P2=300 mg/mL ozonated Aloe vera (OAV), P3=600 mg/mL OAV, P4=1200 mg/mL OAV. The expression of PDGF was assessed using Allred scoring with immunohistochemical staining, whereas the epidermal thickness was assessed using hematoxylin and eosin (H&E) staining at 400x microscopic magnification. The PDGF expression and epidermal thickness between the control and the treatment groups showed significant differences using a Kruskal-Wallis test (P=0.001) and one-way ANOVA test (P<0.001). The groups that was given ozonated Aloe vera oil had higher average of PDGF expression and thicker epidermis than the other groups. The Spearman's correlation test showed a strong positive relationship (p<0.001 and r=0.709) between the two variables. In conclusion, ozonated Aloe vera oil improves healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness.
Soursop Leaf Extract Reduces AST, ALT, Bilirubin Levels, and Liver Damage Scores in Sorafenib-treated Wistar Rats with Hepatocellular Carcinoma Susilaningsih, Neni; Prajoko, Yan Wisnu; Budijitno, Selamat; Prabowo, Erik; Istiadi, Hermawan; Muniroh, Muflihatul; Riwanto, Ignatius
The Indonesian Biomedical Journal Vol 18, No 1 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i1.3883

Abstract

BACKGROUND: Sorafenib, the standard therapeutic agent for advanced hepatocellular carcinoma (HCC), may induce hepatic dysfunction, thereby necessitating adjunctive therapy to mitigate this adverse effect. While preliminary research has suggested that Soursop (Annona muricata) leaves exhibit anti-tumor and hepatoprotective properties, their efficacy in mitigating liver damage associated with sorafenib treatment remains unexplored. This study was conducted to assess the liver-protective effects of soursop leaf extract in Wistar rats receiving sorafenib for HCC treatment.METHODS: Ethanol extract of soursop leaves was prepared using the maceration method. Twenty-nine Wistar rats were divided into five groups: healthy control (HC) group, HCC groups receiving no treatment, sorafenib only, sorafenib + 50 mg/kgBW/day soursop extract, and sorafenib + 100 mg/kgBW/day soursop extract. All groups, except the HC group, were given Diethyl Nitrosamine (DEN) to cause HCC. Following a two-week treatment period, serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assessed using colorimetric methods, while total bilirubin was assessed using diazo with sulphanilic acid method. From histopathological specimen, relative liver weight was measured and liver damage score was assessed using Hematoxylin and Eosin.RESULTS: Administration of sorafenib resulted in a reduction of AST, ALT, total bilirubin, relative liver weight, and liver damage scores. Furthermore, the combined administration of sorafenib with soursop leaf extract at dosages of 50 and 100 mg/kgBW/day led to a dose-dependent amelioration of these indicators. The most pronounced improvement was observed with the highest dose of soursop extract, which significantly reduced AST, ALT, total bilirubin, relative liver weight, and liver damage scores compared to the sorafenib-only group.CONCLUSION: Soursop leaf extract at 100 mg/kgBW/day effectively reduced AST, ALT, bilirubin levels, and liver damage score in sorafenib-treated Wistar rats with HCC, indicating its hepatoprotective effects. These findings suggest that soursop leaf extract may be a promising adjuvant therapy for mitigating sorafenib-induced hepatotoxicity in HCC treatment.KEYWORDS: Annona muricata, hepatocellular carcinoma, sorafenib, AST, ALT, bilirubin, hepatoprotective
Co-Authors , Yan Wisnu Prajoko Abdul Hakam Abdul Mughni Abraham Simatupang Aditya Purnama Agnes Stella Valentina Akhmad Ismail Almaz, Ayyasi Izaz Ambariyanto , Ar, Ardiyana Ari Suwondo Ariyanto, Diaza O. Asri, Hairul Astika Widy Utomo, Astika Widy Awal Prasetyo Azhar, Abu Bambang Joni Karjono Bambang Witjahjo Bambang Witjahjo Bernadus Parish Budiono Budiono, Bernardus Parish Bulandari, Beatrice LA. Cahyono, Chemy Wiryawan Catharina Suharti Damma Purnawati, Ratna Desy Armalina Dimarti, Safira Chairani Dwi Marliyawati Elvira Yunita Endang Sri Lestari Eriawan Agung Nugroho Eriawan Agung Nugroho, Eriawan Agung Eva Annisa Fahmi Syarif Fanti Saktini Ferry Sandra Fidela Hanan Zivana Fifin Luthfia Rahmi Fuadi, Ahmad F. Hardhono Susanto Hardian Hardian Hari Peni Julianti Helmia Farida Hermawan Istiadi Iffiyana, Alifatul Ignatius Riwanto, Ignatius Indah Saraswati Joni Karjono, Bambang Kanti Yunika Kurniaty, Linggom Kusuma, Nauval Marta Mahabuana, Bintang Mahendra, Vito Maria Eka Patri Y Maria Mexitalia Mario Sadar Bernitho Hutagalung Maryam Restiwijaya Meiny Suzery Muhammad Nur Mulyadi Djojosaputro, Mulyadi Muniroh, Muflihatul Muttaqien, Rizal Arief, Najatullah Najatullah Najatullah, Najatullah Nani Maharani Nugrahadi, Dimas Erlangga Nugroho, Trilaksana Parish Budiono Prabowo, Erik Prihharsanti, Christina H.N. Pritadesya, Maharani Prizka Purnawati, Ratna Dama Purnomo, Hery D. Putri, Ajeng Kurniasari Rahajeng, Herera Rampengan, Derren DCH. Ratna Damma Purnamawati Ratna Damma Purnawati Renni Yuniati Respati, Danendra RK. Retno Murwani Ryco Giftyan Ardika Selamat Budijitno Sigit Adi Prasetyo Sigit Adi Prasetyo, Sigit Adi Silaban, Hertina Sri Susilowati Suhartono, Suhartono Supandi Andy SyarofilAnam, Moh. Tanti Ajoe Kesoema, Tanti Ajoe Tjandra, Kevin C. Tobing, Romauli Tri Widyawati, Tri Trilaksana Nugroho Udadi Sadhana, Udadi Ulfa Nurullita Utami, Widoasti Putri Wahyu Haris Prabowo Widiastiti, Nyoman Suci Yan Wisnu Prajoko Yuriz Bakhtiar Zhafran Hafizhki