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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Kedokteran Brawijaya Jurnal Ilmu dan Teknologi Hasil Ternak The Journal of Experimental Life Sciences (JELS) AGRIKA Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Mahasiswa Manajemen Sumberdaya Perairan Wacana, Jurnal Sosial dan Humaniora Research Journal of Life Science REKAYASA Journal of Mechanical Engineering Science and Technology Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan Indonesian Journal of Natural Pigments Journal of Aquaculture and Fish Health Syntax Idea Jurnal Karinov Jurnal Abdi Insani Jurnal Pengabdian, Pendidikan dan Teknologi Teknomekanik JURNAL BAHARI PAPADAK Livestock and Animal Research Jurnal Penelitian Chanos Chanos Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Yanuhar, Uun
Aquatic Resources Management, Fisheries And Marine Science Faculty, Universitas Brawijaya
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The Use Nanoparticles Tomatoes Flour As Source of Fiber on Chicken Nuggets Herly Evanuarini; Agus Susilo; Uun Yanuhar; Adelya Desi Kurniawati
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 17 No. 3 (2022)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2022.017.03.4

Abstract

The purpose of this research was to determine the use nanoparticle tomatoes flour as source fiber to increased the quality of chicken nuggets. The materials used in research are nuggets made from chicken meat with optional ingredient other flour tapioca, pepper, onion white, salt, sugar and bread flour.  The addition of nanoparticles of tomato flour on chicken nuggets by treatment carried out. Experimental method laboratory used as a method test laboratory. The Completely Randomized Design was used as an experimental design with 4 treatments and 5 replications. The treatment use control that is without the addition of nanoparticles tomato flour (P0), P1 (addition of nanoparticles tomato flour 1%), P2 (2%), and P3 (3%) of the total chicken meat used. The variables measured are protein content, fiber content, pH value, and sensory quality (color, taste, aroma, texture, and acceptance). The addition of nanoparticles tomatoes flour (Lycopersicum esculentum Mill) on chicken nuggets gives highly significant effect (P<0.01) on protein content, fiber, pH value, and sensory quality of color, taste, aroma, texture, and acceptance. Protein content in chicken nuggets with addition nanoparticles tomatoes flour (Lycopersicum esculentum Mill) produced range between 13.1 – 16.17. The average rate of chicken nuggets fiber range between 0.79 – 1.04%. Chicken nuggets pH value between 5.86 – 6.16. Panelists score on quality organoleptic color chicken nuggets addition nanoparticles tomatoes flour range between 3.15 – 4.50, score taste ranges between 3.25 – 4.65, score aroma ranges from 3.20-4.55 texture range 3.15 – 4.60 dan acceptance 3.40 – 4.70. The research conclusion is the addition of tomatoes flour in the form of nanoparticles could improved the quality of nuggets and additions by 3 percent make chicken nuggets with best quality.
Exploration of Brachionus sp. Protein on the In Vivo Response of CD4 at Cantang Grouper (Epinephelus sp.) with VNN Infection (Viral Nervous Necrosis) Nur Fauziyah Martiningsih; Uun Yanuhar; Mohammad Musa; Dwi Retna Kumalaningrum
Journal of Aquaculture and Fish Health Vol. 12 No. 3 (2023): JAFH Vol. 12 No 3 September 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i3.31497

Abstract

This study examines the function of Brachionus sp. protein on the Cluster Differentiation-4 (CD4) immune system in cantang grouper infected with VNN. The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of CD4 as an indicator of increased fish body defense system against VNN. Brachionus sp. itself contains three protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa, which are expected to increase the immune system against VNN on cantang grouper. Protein injection of Brachionus with doses of 35 µl, 105 µl and 170 µl /150 g cantang grouper, showed that the lowest decrease was at a dose of 105 µl/150 gram cantang groupers.
Antibody Response in The Blood of Cantang Grouper Infected by Viral Nervous Necrosis with Recombinant Protein Chlorella Vulgaris Vaccination Choirul Huda; Uun Yanuhar; Muhammad Musa; Yusuf Wahyudi; Nur Sakinah Junirahma; Nico Caesar
Journal of Aquaculture and Fish Health Vol. 12 No. 2 (2023): JAFH Vol. 12 No. 2 June 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i2.39231

Abstract

Cantang grouper (Epinephelus sp.) is one of the Export Oriented commodities which is a mainstay in increasing Indonesian mariculture production. The disease that causes many deaths in cantang grouper is Viral Nervous Necrosis. One of the most effective ways is by giving vaccines to fish. This study aims to develop a recombinant protein Chlorella vulgaris vaccine with the addition of an adjuvant to increase the immunity of cantang grouper infected with VNN. The method used was experimental with a Completely Randomized Design (CRD) consiting of seven treatments and three replications, namely (K+) cantang grouper infected with VNN, (T1) Healthy cantang grouper was given 33 µl of vaccine, (T2) Healthy cantang grouper was given vaccine of 66 µl, (T3) Healthy cantang grouper was given 112 µl of vaccine, (T4) Healthy cantang grouper was given 33 ul of vaccine and then tested against Viral Nervous Necrosis, (T5) Healthy cantang grouper was given 66 ul vaccine and then tested against Viral Nervous Necrosis, (T6) Healthy cantang grouper was given 112 ul vaccine and then tested against Viral Nervous Necrosis. The fish used were 8-10 cm in size and were reared for 56 days. The results showed that the Chlorella vulgaris protein recombinant vaccine with the addition of adjuvant gave a very significant effect on the immunity of cantang grouper infected with VNN with the best dose of 33 µl (T4) seen from the relative protection level (RPS) (75,6%), survival of cantang grouper (78%) after challenge test and a significant increase in antibody titer.
Pengaruh Nanovaksin Berbasis Nanopartikel Hybrid Chitosan Terhadap Sel Darah Ikan Kerapu Cantang Yang Diinfeksi VNN Gian Suryanatha Hartawan; Uun Yanuhar; Muhammad Musa; Heru Suryanto; Gunanti Mahasri; Apri Supii; Nico Rahman Caesar
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 4 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.4.650

Abstract

Cantang grouper (Epinephelus sp.) is a fish with a high economic value and has been widely cultivated. VNN disase, caused by Betanodavirus infection, can lead to mass mortality of cantang grouper. Precautions considered effective in preventing Viral nervous necrosis (VNN) disease include vaccination. This study aimed to determine the effect of administering recombinant Chlorella vulgaris protein nanovaccine based on chitosan hybrid nanoparticles in increasing the immune response of cantang grouper infected with VNN. The study consisted of five treatments: K+ (cantang grouper infected with VNN), K- (healthy cantang grouper), T1 (healthy cantang grouper given 33 µl of nanovaccine and then challenged with VNN), T2 (healthy cantang grouper given 66 µl of nanovaccine and then tested with a VNN challenge), and T3 (healthy cantang grouper given 112 µl of nanovaccine and then tested with a VNN challenge). The parameters observed were haematology, nanovaccine Relative percent survival (RPS), and Survival rate (SR) levels. The study results showed that administering the nanovaccine increased the immune response of the fish, as indicated by an increase in haematology response, RPS, and SR in cantang grouper compared to the treatment without nanovaccine administration. The best dose of nanovaccine in this study is 33 µl. Keywords: fish disease, nanovaccines, virus, C. vulgaris, grouper
Viral Nervous Necrosis and Vibriosis in Grouper Fish: A Case Study Gian Suryanatha Hartawan; Uun Yanuhar; Muhammad Musa; Amira Baihani; Yusuf Arif Wahyudi; Choirul Huda; Nico Rahman Caesar
Journal of Aquaculture and Fish Health Vol. 12 No. 3 (2023): JAFH Vol. 12 No 3 September 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i3.40256

Abstract

Viral Nervous Necrosis (VNN) and Vibriosis are contagious diseases that can affect grouper fish from the larval to adult stages and cause significant economic losses for farmers. The owner brought the sunuk grouper to BKIPM Mataram to determine the fish's health. Since the grouper arrived at the floating net cage, the owner reports it has sustained bodily injuries. The grouper is separated from the healthy fish and placed in the area where the sick fish are. During maintenance, the fish exhibits a decrease in appetite, wounds on the mouth, operculum, and body from the head to the tail, low mobility, and swimming upside-down. The physical examination revealed that the fish was swimming limply in an inverted position and had lesions on various body parts. According to virological and bacteriological laboratory tests, the sunuk grouper infected with Vibriosis and VNN can be affected by the condition of fish that have been sick since they first arrived. Also, stress, contact with infected fish, and a decreased appetite result in sunuk grouper contracting the disease. Due to a disease, a fish's immune system does not function optimally. This condition makes sunuk susceptible to several infectious diseases.
The Relationship Between Water Quality and Phytoplankton Abundance with Different Showing Density in Litopenaeus vannamei Ponds in Bayeman Village, Probolinggo District, East Java Fika Fitrianesia; Asus Maizar Suryanto Hertika; Uun Yanuhar; Muhammad Faiq Ash Shiddiq
Jurnal Penelitian Pendidikan IPA Vol. 10 No. 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.4364

Abstract

Intensive ponds often experience a decrease in water quality and cause ponds to bloom due to the fertility of the pond bottom due to the buildup of organic material originating from leftover feed, feces and the remains of dead shrimp. The aim of this research is to determine the relationship between water quality and phytoplankton abundance in intensive pond types with different densities in Bayeman Village, Probolinggo Regency. The research was carried out from January to March 2023. Samples were taken using the purposive sampling method in 6 different pond plots. Phytoplankton analysis was carried out at the Laboratory of the Sand Source Freshwater Fisheries Technical Implementation Unit and the Hydrobiology Laboratory of the Fish Resources Section of the Faculty of Fisheries and Marine Sciences. Measurement of in situ water quality parameters, namely temperature, pH, brightness, DO, TDS, light intensity, salinity and ex situ nitrate, phosphate and ammonia using spectrophotometric methods. The correlation test results of physico-chemical parameters related to phytoplankton abundance have a strong and heterogeneous relationship only with the orthophosphate parameter
PENERAPAN KOLAM KARANTINA IKAN HIAS BERBASIS TEKNOLOGI RAMAH LINGKUNGAN DI POKDAKAN MAKMUR REJEKI KABUPATEN TULUNGAGUNG Uun Yanuhar; Muhammad Musa; Herly Evanuarini; Dyah Kinasih Wuragil; Heru Suryanto; Muhammad Sumsanto; Nico Rahman Caesar
Jurnal Abdi Insani Vol 10 No 4 (2023): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v10i4.1141

Abstract

The goldfish (Carassius auratus) has become one of the most sought-after ornamental fish today. The continuously rising market demand for goldfish as pets and decorative objects has driven the growth of the goldfish (Carassius auratus) farming industry. However, fish farmers still face difficulties in controlling the health of the fish and the quality of their water. The aquatic environment plays a crucial role in the growth of goldfish (Carassius auratus). Therefore, environmental management and fish health are essential in goldfish (Carassius auratus) farming. After the PKM Team analyzed the existing issues, the activities carried out to address these issues include the implementation of an Environmentally Friendly Recirculating Aquaculture System (RAS) in the goldfish (Carassius auratus) farming ponds. In this PKM program, water filtration will consist of mechanical filters, biological filters, chemical filters, and UV light. The results of the community service activities conducted by the PKM Team with their partners include increased knowledge, improved skills, increased production capacity, and increased profitability. Keywords:
Fungsi Sel Imun Interleukin-4 (IL-4) pada Ikan Kerapu Tikus (Cromileptes altivelis) dengan Induksi Protein Imunogenik Vibrio harveyi [The Function Of Interleukin-4 (IL-4) Immune Cells On Humpback Grouper (Cromileptes altivelis) By Induction Of Immunogenic Protein Of Vibrio harveyi ] Uun Yanuhar; Asus Maizar SH; Ita Fitria S
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11588

Abstract

Abstract Humpback grouper (Cromileptes altivelis) is a commodity to be cultivated because it has a high price, but the grouper highly susceptible to Vibriosis (Vibrio harveyi). The research objective was to see the function of immune cells of interleukin-4 (IL-4) are formed due to the induction of immunogenic proteins of Vibrio harveyi. Research procedures include Vibrio harveyi immunogenic protein isolation, SDS-PAGE electrophoresis, elektroelusi and dialysis, clinical exam of Vibrio harveyi immunogenic proteins, and immunohistochemical to look at immune cells (cells IL-4) with using a secondary antibody labeling of anti IL-4 biotin conjugate. The results showed that Vibrio harveyi protein found have immunogenic properties is 51.16 kDa. This protein is an adhesin protein. Protein 51.16 kDa was purified by electroelution and the protein will be used as a vaccine administered by intraperitoneal injection in grouper (Cromileptes altivelis) at a dose of 31.6 ug protein / 150 g of fish weight. In organ tissues of the brain, eyes and kidneys of induced fish by immunohistochemical examination, It was detected Immune cells (cells IL-4) using a secondary antibody labeling of anti IL-4 biotin conjugate. Tues formed IL-4 can be seen on the network grouper indicated by a change in tissue color with an inverted microscope examination of the golden brown color is formed on the IL-4 immune cells. It show the cross-reaction between 51.16 kDa immunogenic protein induced in fish with anti IL-4 biotin conjugate. The conclusions is the protein of Vibrio harveyi with molecular weight of 51.16 kDa is an immunogenic protein which able to induce formation of IL-4 in grouper.
Pengaruh Pemberian Bahan Aktif Ekstrak Nannochloropsis oculata Terhadap Kadar Radikal Bebas Pada Ikan Kerapu Tikus (Cromileptes altivelis) yang Terinfeksi Bakteri Vibrio alginolyticus [The Influence Of Supply Of Bioactive Extract Nannochloropsis oculata To Dose Of Free Radicals In The Humpback Grouper (Cromileptes altivelis) That Was Infected By Vibrio alginolyticus] Uun Yanuhar
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 2 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i2.11687

Abstract

AbstractNanochloropsis oculata is one of kind green algae that overload in Indonesia ocean. The purpose of this research  was to know the potency antioxidant of Nannochloropsis oculata extract as in the Humback Grouper. The research was conducted by  experimently and used direct random sampling as design with 4 experiments and 3 repeats  i.e control fish, fish was  added Nannochloropsis oculata extract, Vibrio alginolyticus - infected fish, and Vibrio alginolyticus - infected fish that was added Nannochloropsis oculata extract. The result showed, fish that added by Nannchloropsis oculata extract made a different influence which was very clear to the decreasing of  free radical in the body. Free radical (malonaldehid) in the Vibrio alginolyticus -  infected fish was 4, 04 µg/100 mg which fish was added by Nannochloropsis oculata extract, the free radical level was decrease into 3, 29 µg/100 mg massa.
Mekanisme Infeksi Vibrio Pada Reseptor Ikan Kerapu Tikus Cromileptes altivelis [The Mechanism Of Vibrio Infection To Grouper Reseptor Cromileptes altivelis] Uun Yanuhar
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11693

Abstract

Abstract One of the most favourite fish that farmer fish cultivated because its high economic value was gouper (C. altivelis). The obstacle in cultivation system was caused by Vibrio algynolitycus. Vibrio angullarum, Vibrio parahaemolyticus. Vibrio harveyi infection. Many deseases that attacked to the grouper was caused by the role of pathogen specific as a ligan and its reseptor. The aim of these study was to improve and to know the role of reseptor in vibriosis mechanism with tested spesific reseptor protein of grouper Cromileptes altivelis. These study used experimental and descriptive methode with identified of grouper reseptor, Hemaglutinin test and observation use electron microscope. These study result showed that reseptor protein of C. altivelis was found in its eyes. These showed spesivity and reactivity to the vibriosis specific epitop.
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Alfarisi, Muhammad Asnin Alfi Nur Rusydi Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Masitha Anasiru, Arief Farhan Kurniawan Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Annisa Fitri Anggreini Apri Supii Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Baihani, Amira Bili Darnanto Susilo BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Defa Rizqi Machfuda Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Ekwan Nofa Wiratno Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Fitrianesia, Fika Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gilang Rusrita Aida Gilang Rusrita Aida Gunanti Mahasri Hardoko Hardoko Hartati Kartikaningsih Herlina, Cucun Herly Evanuarini Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Islamy, R Adharyan Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Kharisma Nuria Fitri Kristiani, Maria Lutfi Ni`&#039;Matus Salamah Lutfia Fahimatul Ilmi MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marsono Marsono Martiningsih, Nur Fauzi-yah Martiningsih, Nur Fauziyah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Fadjar Mohammad Musa Mufidah, Rhobithotus Muhamad Muhajir Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nezya Pramudya Wardani Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari, Septi Setyayunita, Tamaryska Shiddiq, Muhammad Faiq Ash Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Umi Zakiyah Utari, Inpita Casuarina Eqisetia Wahyudi, Yusuf Arif Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Wahyudi Zainal Abidin