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Potential of Dayak Onion (Eleutherine palmifolia (L) Merr) Extract As Antibacterial Against Pseudomonas fluorescens Fransira, Immaria; Yanuhar, Uun; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1074.218 KB) | DOI: 10.21776/ub.jels.2019.009.02.03

Abstract

Dayak onions (E. palmifolia (L) Merr) is a herb used as medicine and known to have antibacterial activity. This research aimed to evaluate antibacterial activity of Dayak onion (Eleutherine palmifolia (L) Merr) extract against fish pathogen Pseudomonas fluorescens. The method used in this study was extraction by maceration, UV-Vis spectrophotometry analysis on the extract and antibacterial activity test with Minimum Inhibitory Concentration (MIC) as well as disc diffusion test. The results of this study showed that the yield value was 8.87%. Based on UV-Vis analysis, it confirmed that extract of Dayak onion (Eleutherine palmifolia (L) Merr) contains predominantly flavonoids and phenols with its derivatives, that have antibacterial activity. The results of MIC and disc diffusion test showed potential antibacterial activity against P. fluorescens, as observed from the value of absorbance and inhibition zones that formed. Keywords: Antibacterial, Disc diffusion, Eleutherine palmifolia (L) Merr, MIC, Pseudomonas fluorescens.
The Effect of Protein Induction of Brachionus sp. Toward In-Vivo Response of Cluster Differentiation-8 (CD8) Cantang Grouper (Epinephelus sp.) Infected by VNN (Viral Nervous Necrosis) Nur Fauziyah Martiningsih; Uun Yanuhar; Mohammad Musa; Dwi Retna Kumalaningrum
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4025

Abstract

The large demand for grouper conduce increase production intensively in various ways, by increasing the production of grouper aquaculture raises causing new problems, one of which is infection with VNN disease. The use of antibiotics to treat VNN infections is prohibited or no longer recommended by the government. An alternative way to deal with VNN infection is by increasing the body's immunity in the fish itself. One effort that can be done to increase the body's immunity in grouper is by administering Brachionus sp. protein extracts. This research was conducted to determine whether the Brachionus sp. has an effect on the immune system in cantang grouper (Epinephelus sp.) as indicated by the emergence of Cluster Differentiation-8 (CD8) expression in fish that have been infected with VNN. The proteins contained in Brachionus sp. that has three type molecular weight (122.73 kDa, 75.49 kDa, and 13.77 kDa) potential as an ingredient for treating VNN-infected fish. It is hoped that this can improve the immune system in Epinephelus sp. infected with VNN. Protein Brachionus sp. given by injection at a dose of 35 µl, 105 µl and 170 µl per 150 gr Epinephelus sp. 105 µl was the best concentration in boosting the immune system in the tested Epinephelus sp., as indicated by a decreased CD8 count.
The Relationship Between Water Quality and Phytoplankton Abundance with Different Showing Density in Litopenaeus vannamei Ponds in Bayeman Village, Probolinggo District, East Java Fika Fitrianesia; Asus Maizar Suryanto Hertika; Uun Yanuhar; Muhammad Faiq Ash Shiddiq
Jurnal Penelitian Pendidikan IPA Vol 10 No 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.4364

Abstract

Intensive ponds often experience a decrease in water quality and cause ponds to bloom due to the fertility of the pond bottom due to the buildup of organic material originating from leftover feed, feces and the remains of dead shrimp. The aim of this research is to determine the relationship between water quality and phytoplankton abundance in intensive pond types with different densities in Bayeman Village, Probolinggo Regency. The research was carried out from January to March 2023. Samples were taken using the purposive sampling method in 6 different pond plots. Phytoplankton analysis was carried out at the Laboratory of the Sand Source Freshwater Fisheries Technical Implementation Unit and the Hydrobiology Laboratory of the Fish Resources Section of the Faculty of Fisheries and Marine Sciences. Measurement of in situ water quality parameters, namely temperature, pH, brightness, DO, TDS, light intensity, salinity and ex situ nitrate, phosphate and ammonia using spectrophotometric methods. The correlation test results of physico-chemical parameters related to phytoplankton abundance have a strong and heterogeneous relationship only with the orthophosphate parameter
Hematological Analysis of Red Bader Fish (Barbonymus altus) as a Biomarker in Assessing Pollution Status in Brantas River, Surabaya, East Java Arief Farhan Kurniawan Anasiru; Asus Maizar Suryanto Hertika; Uun Yanuhar
Jurnal Penelitian Pendidikan IPA Vol 10 No 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.5659

Abstract

The Brantas River has many functions for both humans and the aquatic organisms in it. The type of fish that is often found in the Brantas River in Surabaya is the red bader fish (Barbonymus altus). Hematology is a sensitive parameter and can be used as a marker in assessing water conditions. The aim of this research is to analyze the hematological profile of red bader fish (Barbonymus altus) to assess the condition of the Brantas River, Surabaya. The research was carried out at 3 locations on the Brantas River in Surabaya with 3 sampling times with an interval of 1 month starting in January – March 2022. The parameters observed were water quality (temperature, TSS, TDS, pH, DO, BOD, ammonia and heavy metals Hg) and hematological parameters (erythrocytes, hemoglobin, hematocrit, leukocytes and micronuclei). Analysis of the relationship between water quality parameters and hematology using the Correspondence Correlation Analysis (CCA) method. The results show that there are water quality parameters that do not meet quality standards and there are abnormal hematological parameters. CCA analysis shows that there is a correlation between water quality and hematological parameters with moderate concentrations (erythrocytes, hemoglobin and hematocrit) and strong concentrations (leukocytes and micronuclei).
Analisis Status Kualitas Air di Sungai Porong yang Tercemar oleh Deterjen terhadap Respon Hematologi Ikan Java Barb (Barbonymus gonionotus) Nico Rahman Caesar; Uun Yanuhar; Abd. Rahem Faqih; Septi Anitasari; Gatot Ciptadi; Muhammad Musa; Mohammad Bisri; Muhammad Sumsanto; Nezya Pramudya Wardani
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6170

Abstract

The Porong River is one of the rivers that faces high anthropogenic impacts, such as surfactant waste from detergents. The hematology of Javanese carp (Barbonymus gonionotus) serves as a sentinel organism that can be used as a biomarker for the waters of the Porong River. The aim of this study is to analyze the water quality status of the Porong River polluted with surfactants using the STORET method, as well as its impact on the hematology of Javanese carp using regression and correlation analysis. The research employed a descriptive survey method. The results of the study indicated that the Porong River was categorized as moderately polluted. The average highest surfactant concentration reached 7.2 mg/L, leading to an increase in TSS to 107.95 mg/L and a decrease in DO to 1.80 mg/L. Surfactants exhibited a negative correlation with erythrocytes (91.2%), hematocrit (47.7%), and hemoglobin (60.6%), indicating that the higher the surfactant concentration, the lower the number of erythrocytes, hematocrit levels, and hemoglobin levels in Javanese carp. Conversely, there was a positive correlation between surfactants and leukocytes (56.7%) and blood glucose (66.7%), suggesting that the higher the surfactant concentration, the higher the number of leukocytes and blood glucose levels in Javanese carp.
Improvement of physical and sensory quality of beef patties with the addition of chicken eggshells nanopowder Evanuarini, Herly; Susilo, Agus; Yanuhar, Uun; Kurniawati, Adelya Desi
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.77733

Abstract

Objective: The purpose of this study was to investigate the effect of adding chicken eggshells nanopowder for the physical quality and sensory evaluation of beef patties.Methods: The method used in this study was a laboratory experiment with a completely randomized design with 5 treatments: without adding chicken eggshells nanopowder as a control, adding 0.1%, 0.3%, 0.5% and 0.7% chicken eggshells nanopowder (w/w) with 5 repetitions. Data were analyzed by analysis of variance, if there was a significant or very significant difference it was continued with Duncan's multiple range test.Results: Based on the research results, it was found that the addition of chicken eggshells nanopowder to beef patties had a very significant effect (P<0.01%) on moisture content, water holding capacity, cooking loss, texture, and sensory evaluation.Conclusions: The addition of 0.7% chicken eggshells nanopowder can reduce moisture content, increase water holding capacity, reduce cooking loss, improve texture, and sensory quality acceptable to panelists.
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Agus Susilo Alfarisi, Muhammad Asnin Alfi Nur Rusydi Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Masitha Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Annisa Fitri Anggreini Apri Supii Arief Farhan Kurniawan Anasiru Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Baihani, Amira Bili Darnanto Susilo BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Defa Rizqi Machfuda Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Ekwan Nofa Wiratno Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gilang Rusrita Aida Gilang Rusrita Aida Gunanti Mahasri Hardoko Hardoko Hartati Kartikaningsih Herlina, Cucun Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Islamy, R Adharyan Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Kharisma Nuria Fitri Kristiani, Maria Lutfi Ni`&#039;Matus Salamah Lutfia Fahimatul Ilmi MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marsono Marsono Martiningsih, Nur Fauzi-yah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Fadjar Mohammad Musa Mohammad Musa Mufidah, Rhobithotus Muhamad Muhajir Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nezya Pramudya Wardani Nezya Pramudya Wardani Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari Septi Anitasari, Septi Setyayunita, Tamaryska Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Umi Zakiyah Utari, Inpita Casuarina Eqisetia Wahyudi, Yusuf Arif Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Wahyudi Zainal Abidin