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Yanuhar, Uun
Aquatic Resources Management, Fisheries And Marine Science Faculty, Universitas Brawijaya
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PELATIHAN DAN PENDAMPINGAN MANAJEMEN KUALITAS AIR DAN KESEHATAN PADA BUDIDAYA IKAN KOI (CYPRINUS CARPIO) Uun Yanuhar; Muhammad Musa; Dyah Kinasih Wuragil
Jurnal KARINOV Vol 2, No 1 (2019): Januari
Publisher : Institute for Research and Community Service (LP2M), Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.227 KB) | DOI: 10.17977/um045v2i1p69-74

Abstract

Ikan koi (Cyprinus carpio) merupakan salah satu komoditas ikan hias air tawar yang memiliki nilai ekonomi dan peminat yang sangat tinggi. Hal ini menjadi dorongan untuk para pembudidaya untuk meningkatkan usaha budidaya ikan koi. Kecamatan Nglegok adalah salah satu lokasi kawasan Minapolitan Nasional dengan komoditas unggulannya berupa ikan koi. Namun, dalam berbudiya ikan koi  sering terjadi masalah salah satunya adalah timbulnya penyakit pada ikan. Penyakit yang sering menjadi kendala dalam kegiatan budidaya ikan koi di Kecamatan Nglegok adalah penyakit Myxobolus sp. Infeksi Myxobolus sp. pada ikan menyebabkan terganggunya proses pernafasan hingga kematian. Dalam mengatasi permasalahan tersebut dilakukan beberapa upaya berupa penerapan IPTEK mengenai manajemen kesehatan untuk budidaya ikan koi melalui kegiatan Penyuluhan Manajemen Kualitas Air dan Kesehatan Ikan Koi, Pendampingan Pemeriksaan Kualitas Air, Pendampingan Pemeriksaan Penyakit Ikan Koi dan Pelatihan Penggunaan Probiotik. Berdasarkan pengembangan penanganan permasalahan tersebut, hasil yang diperoleh dari program ini bahwa seluruh anggota mitra hadir dan mengikuti kegiatan penyuluhan yang diadakan. Melalui program ini pula para anggota mitra mendapatkan pembekalan materi edukasi dalam melakukan kegiatan berbudidaya ikan koi agar di masa depan dapat mengatasi permasalahan yang terjadi dalam budidaya ikan koi sehingga kerugian yang terjadi akibat permasalahan dalam budidaya ikan koi berkurang dan perekonomian anggota mitra meningkat.
Coastal Rural Development Planning: Fishery Food Management Rudianto Rudianto; Uun Yanuhar
Wacana Journal of Social and Humanity Studies Vol. 21 No. 4 (2018)
Publisher : Sekolah Pascasarjana Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The planning of coastal villages based on fishery food management is intended as an approach to strengthen coastal communities to alleviate rural poverty. The pressure on the coast from both anthropogenic processes causes the declining of fishery food supplies. The purpose of this study is to assess the relationship among variables affected fishery food in coastal villages, and to choose the priority to solve degradation of fishery food supplies. Case studies were selected at Tamban beach, Malang Regency. The research method used Partial Least Square (PLS) and Analytical Hierarchy Process (AHP) approach. The results show that the coastal rural development planning based on fishery food management is strongly depending on the relationship among the agricultural environment, alleviating rural poverty and fishery food management. The priorty parameter for  the agricultural  environment is that the role of private sector and handling skyrocketing food prices. whereas, the priority solution for rural poverty is that supply access on education and health as well as supplying growth opportunity for job creation. Furthermore, the priority for fishery food management is to increase capacity of local people capacity to prepare management plan and the second priority is that the government provide policy and regulatory framework.
EFEK PEMBERIAN PROBIOTIK TERHADAP IKAN KOI (Cyprinus carpio) YANG TERINFEKSI Myxobolus sp. (Effect of Probiotics Treatment on Koi Carp (Cyprinus carpio) Infected With Myxobolus sp.) Nico Rahman Caesar; Uun Yanuhar; Muhammad Musa
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 1 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.501 KB) | DOI: 10.14710/ijfst.15.1.60-65

Abstract

Myxobolus merupakan ektoparasit yang berbahaya dan dapat mengakibatkan kematian hingga 80%. Dalam praktek akuakultur, probiotik telah digunakan dalam beberapa tahun terakhir probiotik menjadi bagian integral dari praktik budaya untuk meningkatkan pertumbuhan dan ketahanan terhadap penyakit. Tujuan penelitian ini mengetahui pengaruh pemberian probiotik terhadap hematologi dan respon imun ikan koi yang terserang Myxobolus sp. yakni eritrosit dan leukosit serta ekspresi Nuclear Factor-kappa Beta  (NF-kB). Metode yang digunakan yaitu metode eksperimen. Dalam penelitian ini dibagi ke dalam 4 perlakuan yaitu, perlakuan (A) Kontrol, (B) ikan koi terinfeksi Myxobolus sp., (C) ikan koi terinfeksi Myxobolus sp. dengan pemberian probiotik dosis 0,55 ml dan (D) ikan koi terinfeksi Myxobolus sp. dengan pemberian probiotik dosis 1,1 ml. Metode pemberian perlakuan dengan menambahkan probiotik pada 30 liter air pada bak pemeliharaan. Kemudian dilakukan pengamatan eritrosit dan leukosit. Serta dilakukan Imunohistokimia menggunakan antibodi NF-kB pada jaringan insang. Berdasarkan hasil pengamatan eritrosit diperoleh nilai rata – rata pada perlakuan A sebesar 1.666.667 sel/mm3, perlakuan B sebesar 1.940.000 sel/mm3, perlakuan C sebesar 1.776.667 sel/mm3 dan perlakuan D sebesar 1.836.667 sel/mm3. Hasil pengamatan leukosit diperoleh nilai rata – rata pada perlakuan A sebesar 119.800 sel/mm3, perlakuan B sebesar 492.800 sel/mm3, perlakuan C sebesar 308.533 sel/mm3 dan perlakuan D sebesar 318.400 sel/mm3. Selanjutnya, berdasarkan hasil Imunohistokimia  didapatkan hasil pada perlakuan A nilai DAB sebesar 15,1%, perlakuan B nilai DAB sebesar 31,7%, perlakuan C nilai DAB sebesar 53,5% dan pada perlakuan D nilai DAB sebesar 47,5 %. Dari penelitian ini dapat disimpulkan bahwa pemberian probiotik berpengaruh terhadap eritrosit dan leukosit serta dapat meningkatkan ekspresi NF-KB sebagai respon imun pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Dosis optimal pemberian probiotik yaitu pada dosis 0,55 ml. Myxobolus is a dangerous parasite that can kill up to 80% on koi carp pond.. Probiotic intake has been proven to change the composition of the microbiota, and therefore helps in the recovery of microbiota that are disrupted (by antibiotics or other risk factors) into favorable or normal compositions. The purpose of this study was to determine the effect of probiotic administration on hematology and the immune response of koi fish attacked by Myxobolus sp. namely erythrocytes and leukocytes and expression of Nuclear Factor-kappa Beta (NF-kB). The method used was an experimental by providing probiotics to fish that promoted Myxobolus sp. In this study it was divided into 4 treatments namely, (A) Control, (B) koi carp infected by Myxobolus sp., (C) koi carp infected by Myxobolus sp. with probiotic doses of 0.55 ml and (D) koi fish infected by Myxobolus sp. by administering a probiotic dose of 1.1 ml. The treatment methods were the fish immersed into 30 L of water that added by Probiotic. The hematological observation of koi fish,  erythrocytes and leukocytes, was observed and immunohistochemistry using NF-KB antibodies in the gill tissue which is the target of the entry of Myxobolus sp. on Koi fish. Based on the results of the treatment of erythrocytes obtained the value of A handling is 1,666,667 cells / mm3, handling B is 1,940,000 cells / mm3, handling C is 1,776,667 cells / mm3 and handling D is 1,836,667 cells / mm3. The results of leukocyte observations obtained an average value on treatment A of 119,800 cells / mm3, treatment B was 492,800 cells / mm3, treatment C was 308,533 cells / mm3 and treatment D was 318,400 cells / mm3. Furthermore, based on the results of Immunohistochemistry, the results of handling A DAB value of 15.1%, handling B DAB value of 31.7%, handling C DAB value of 53.5% and at the time of treatment D DAB value of 47.5%. From this study it can be concluded that the treatment of probiotics affects erythrocytes and leukocytes and can increase the expression of NF-KB as an immune response in koi fish (Cyprinus carpio) infected with Myxobolus sp. The optimal dose of probiotics is at a dose of 0.55 ml 
UJI IN VITRO DAN KARAKTER PROTEIN EKSTRASELULER (ECP) Edwardsiella tarda DENGAN KONSENTRASI ETHANOL BERBEDA (In Vitro Test and Character of Extracellular Protein (ECP) Edwardsiella tarda with Different Ethanol Concentration) Cucun Herlina; Uun Yanuhar; Maftuch Maftuch
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 2 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.923 KB) | DOI: 10.14710/ijfst.15.2.105-111

Abstract

Protein ekstraseluler (ECP) bakteri memiliki kemampuan imunogenik dan dapat meningkatkan sistem imun tubuh inang. Komponen ECP diantaranya flagellin, protease dan metalloprotease mampu berdifusi pada sel inang untuk mengaktifkan respon imun. Tujuan penelitian adalah untuk mengetahui produksi protein ekstraseluler E. tarda dengan presipitasi ethanol pada konsentrasi berbeda sebagai potensi protein imunogenik secara in vitro. Metode penelitian yaitu kultur bakteri E. tarda, preparasi protein ekstraseluler, presipitasi protein, konsentrasi protein ditentukan denganspektrofotometer dan SDS-PAGE, uji in vitro protein ECP E. tarda, dan uji nilai RPS. Hasil protein ekstraseluler yang diperoleh dengan presipitasi ethanol tertinggi pada konsentrasi 90% sebanyak 5,69 mg/ml dan terendah pada konsentrasi 80% sebanyak 4,81 mg/ml. Hasil karakterisasi protein dengan SDS-SDSPAGE antara 30-60 kDa. Hasil uji in vitro vaksin E. tarda (presipitasi ethanol 100%) baik dari uji viabilitas maupun sterilitas menunjukkan tidak terdapat koloni yang tumbuh. Hasil nilai RPS pada ikan nila (Oreochromis niloticus) mencapai 100% dengan masa pemelihaaraan 9 hari pasca imunisasi. ECP bacteria have immunogenic abilities and can increase the host's immune system. ECP components which are flagellin, protease, metalloprotease can diffuse to host cell for the activation immune response. The study aimed to determine the production of extracellular protein E. tarda with ethanol precipitation at different concentrations as a potential immunogenic protein by in vitro test. The research methods were E. tarda bacterial culture, extracellular protein preparation, protein precipitation, protein concentration determined by spectrophotometer and SDS-PAGE, in vitro ECP E. tarda protein test, and RPS value test. The results of extracellular protein obtained by the highest ethanol precipitation at a concentration of 90% as much as 5.69 mg/ml and the lowest at 80% concentration as much as 4.81 mg/ml. The results of the characterization of proteins with SDS-SDS-PAGE between 30-60 kDa. The results of the in vitro test of E. tarda vaccine (100% ethanol precipitation) from both the viability and sterility tests showed that there were no growing colonies. The results of the RPS in tilapia (Oreochromis niloticus) reach 100% with a maintenance period of 9 days after immunization.
STATUS HEMATOLOGI DAN RESPON IMUN IKAN KOI (Cyprinus carpio) YANG TERINFEKSI Myxobolus sp. DENGAN TREATMENT DIMILIN (Hematological Status and Immune Response of Koi Fish (Cyprinus carpio) Infected by Myxobolus sp. With Treatment of Dimilin) feri Setiawan; Uun Yanuhar; Andi Kurniawan
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 1 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.461 KB) | DOI: 10.14710/ijfst.15.1.80-85

Abstract

Koi merupakan ikan hias favorit di pasar nasional dan internasional. Proses budidaya untuk menghasilkan koi dengan kualitas terbaik masih menjadi kendala, salah satunya adanya infeksi parasit Myxobolus sp. yang dapat menyebabkan kematian massal dalam waktu singkat. Myxobolus yang menyerang jaringan ikan dapat menyebabkan kerusakan prah pada jaringan. Banyak cara dilakukan oleh pembudidaya Ikan Koi untuk menghindari infeksi Myxobolus, yaitu dengan pengobatan secara alami dan menggunakan bahan kimia. Penelitian ini bertujuan untuk mengetahui efek hematologi dan respon imun pada Ikan Koi yang terinfeksi parasit Myxobolus dengan pengobatan dimilin. Metode yang digunakan adalah metode eksperimental dengan perlakuan kontrol ikan sehat (A), Ikan koi yang terinfeksi oleh Myxobolus sp. tanpa perlakuan dimilin (B), ikan koi terinfeksi Myxobolus sp dengan perlakuan dimilin 0,02 mg (C), dan ikan yang terinfeksi Myxobolus sp dengan perlakuan dimilin 0,01 mg (D). Pengambilan darah ikan dilakukan dengan metode punksi pembuluh darah bagian caudal dan dilakukan pengamatan hematologi, serta dilakukan pengamatan imunohistokima dengan melihat persentase Diaminobenzidine (DAB) pada ikan. Hasil rata-rata pada perhitungan hematologi leukosit dan eritrosit tertinggi  ditemukan pada ikan yang terinfeksi Myxobolus sp tanpa perlakuan dimilin (B), dengan eritrosit 2.913.334 sel / mm3 dan leukosit 197.184 sel / mm3. Hasil respon imun berdasarkan pengamatan imunohistokimia didapatkan nilai DAB perlakuan A adalah 16%, nilai DAB perlakuan B 42,7%, nilai DAB perlakuan C 25,6%, dan nilai DAB perlakuan D 31,4%. Kesimpulan penelitian ini adalah pemberian dimilin dapat mempengaruhi respon hematologi dan dapat mempengaruhi respon imun pada ikan yang dapat ditunjukkan dengan Interleukin-6 pada ikan dengan hasil DAB immunoratio. Koi is a favorite ornamental fish in the national and international markets. Cultivation process to produce koi with the best quality is still have problem, one of the problem is parasitic infection of Myxobolus sp. which can cause mass death in a short time. Myxobolus attacks fish tissue can cause severe damage to tissue. Many ways were used by koi fish farmers to avoid Myxobolus infection which  with natural treatment and using chemicals drug. This study aims to determine the hematological effects and immune respons in the koi fish infected with the Myxobolus and treated with dimilin. Experimental method was used in this research with control treatment of healthy fish (A), koi fish infected by Myxobolus sp. without treatment (B), koi fish infected by Myxobolus sp with dimilin treatment 0.02 mg (C), and koi fish infected by Myxobolus sp with dimilin treatment 0.01 mg (D). fish Blood samples were collected with puncture of caudal vessel method and then haematological observations to blood samples, and immunohistokima observations by looking percentage of Diaminobenzidine (DAB) in fish. Highest hematological Results of leukocytes and erythrocytes were found in treatment B, with erythrocytes result 2,913,334 cells / mm3 and leukocyte 197,184 cells / mm3. Immune response result based on immunohistochemical observations with DAB value of treatment A is 16%, treatment B is 42.7%, treatment C is 25.6%, and treatment D is 31.4%. Conclusion in this study that dimilin treatment can affect hematological response and immune response in fish which can be shown with Interleukin-6 in fish with DAB immunoratio results. 
EFEK PEMBERIAN DELTAMETHRIN TERHADAP EKSPRESI CD4 PADA IKAN KOI (Cyprinus carpio) YANG TERINFEKSI Myxobolus sp. (Effect of Deltamethrin on CD4 Expression in Koi Fish (Cyprinus Carpio) Infected by Myxobolus Sp.) Muhammad Sumsanto; Uun Yanuhar; Asus Maizar Suryanto Hertika
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 2 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (692.787 KB) | DOI: 10.14710/ijfst.15.2.94-98

Abstract

Parasit yang sering menyerang ikan koi adalah Myxobolus sp. Ikan yang terinfeksi Myxobolus sp. akan mengalami gejala klinis berupa nodul kemerahan dan pembengkakan pada organ insang. Penelitian bertujuan untuk mengetahui pengaruh pemberian deltamethrin terhadap ekspresi CD4 pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Pada penelitian ini dibagi menjadi 4 perlakuan (A) Kontrol, perlakuan (B) ikan koi terinfeksi Myxobolus sp., perlakuan (C) ikan koi terinfeksi Myxobolus sp. dengan pemberian deltamethrin dosis 0,5 µl/g dan perlakuan (D) ikan koi terinfeksi Myxobolus sp. dengan pemberian deltamethrin dosis 1 µl/g. Kemudian dilakukan pengamatan eritrosit, leukosit dan immunohistokimia untuk mengetahui ekspresi CD4 pada usus. Berdasarkan hasil penelitian didapatkan eritrosit pada perlakuan (A) sebesar 500.000 sel/mm3, (B) sebesar 1.850.000 sel/mm3, (C) sebesar 1.110.000 sel/mm3 dan (D) sebesar 1.670.000 sel/mm3. Hasil pengamatan leukosit perlakuan (A) didapatkan sebesar 121.600 sel/mm3, (B) sebesar 333.550 sel/mm3, (C) sebesar 265.770 sel/mm3 dan (D) sebesar 145.620 sel/mm3. Berdasarkan hasil Imunohistokimia  didapatkan hasil pada perlakuan (A) nilai DAB sebesar 15,0%, (B) DAB sebesar 30,2%, (C) DAB sebesar 24,3% dan (D) DAB sebesar 19,2 %. Dari penelitian ini dapat disimpulkan bahwa pemberian deltamethrin berpengaruh terhadap eritrosit dan leukosit serta dapat menurunkan ekspresi CD4 sebagai respon imun pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Parasites that often attack koi are Myxobolus sp. Fish infected with Myxobolus sp. will experience clinical symptoms of red nodules and swelling in the gill organs. The study aimed to determine the effect of deltamethrin administration on CD4 expression in koi fish (Cyprinus carpio) infected with Myxobolus sp. In this study divided into 4 treatments (A) Control, treatment (B) koi fish infected with Myxobolus sp., Treatment (C) koi fish infected with Myxobolus sp. by treatment a dose of 0.5 µl / g deltamethrin and treatment (D) koi fish infected with Myxobolus sp. with the treatment of 1 µl / g of deltamethrin. Then erythrocytes, leukocytes and immunohistochemistry were observed to determine CD4 expression in the intestine. Based on the results of the study obtained erythrocytes in treatment (A) of 500,000 cells / mm3, (B) of 1,850,000 cells / mm3, (C) of 1,110,000 cells / mm3 and (D) of 1,670,000 cells / mm3. Observation of leukocyte treatment (A) was obtained at 121,600 cells / mm3, (B) of 333,550 cells / mm3, (C) of 265,770 cells / mm3 and (D) of 145,620 cells / mm3. Based on the results of immunohistochemistry the results obtained in treatment (A) DAB value of 15.0%, (B) DAB of 30.2%, (C) DAB of 24.3% and (D) DAB of 19.2%. From this study it can be concluded that the administration of deltamethrin affects erythrocytes and leukocytes and can reduce CD4 expression as an immune response in koi fish (Cyprinus carpio) infected with Myxobolus sp.
IKTIOFAUNA DI HILIR SUNGAI KLAWING KABUPATEN PURBALINGGA, JAWA TENGAH Taufik Budhi Pramono; Diana Arfiati; Maheno Sri Widodo; Uun Yanuhar
Samakia : Jurnal Ilmu Perikanan Vol 9 No 2 (2018): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.59 KB) | DOI: 10.35316/jsapi.v9i2.227

Abstract

The status and potential of ichtyofauna resources in freshwaters, especially downstream of the river, must be monitored and evaluated frequently. Land use along the downstream river is often used as mining and industrial activities. The purpose of this study was to find out ichtyofauna in down stream of the Klawing Purbalingga River in Central Java. The study was conducted in January-March 2017 with descriptive exploratory method. Sampling using gill nets with the help of local fishermen. The fish obtained were identified and grouped by family to the species level. The results showed that in the down stream of the Klawing River there were species diversity consisting of 13 species, 7 families and 13 genera. Ichtyofauna from Cyprinidae family dominate as many as 6 species followed by Bagridae family 2 species. One introduced species of Osphronemus gouramy and one invasive alien fish species Hypostomus plecostomus and other species were indigenous species.
Analysis of The Secondary Metabolite of Kersen Leaf Extracts (Muntingia calabura L.) and Its Potential as Anti-Bacteria to Inhibit Aeromonas hydrophila Gede Angga Krishna Fariestha; Sri Andayani; Uun Yanuhar
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.487 KB) | DOI: 10.21776/ub.rjls.2018.005.02.6

Abstract

Aeromonas hydrophila is a gram-negative bacterium that massively attack aquatic organisms in fresh water, briny water and sea waters. Infection due to this bacterium caused septicemia in the host's body to the point of damaging the body's organs. Hence, alternative material is required in order to cope with this bacterium, which was by using natural material such as kersen leaf (Muntingia calabura L). The purpose of the research is to determine the effect of secondary metabolite contained in kersen leaf as anti-bacterial against Aeromonas hydrophila. This research used a method of MIC (Minimum Inhibitory Concentration) as an anti-bacterial test. In order to examine the secondary metabolite content, phytochemical screening and FTIR (Fourier Transform Infrared Spectroscopy) methods were used. The results obtained through the MIC test of 125 ppm is the minimum concentration capable of inhibiting the growth of Aeromonas hydrophila. Kersen leaf extracts is bacteriostatic anti-bacteria which only inhibit bacterial growth up to 24 hours. Phytochemical screening reported that kersen leaf extracts were positive of flavonoids, tannins, saponins, alkaloids and triterpenoids. Through FTIR test, it is known that phenol compounds were contained in kersen leaf extracts. This phenol is one of the metabolites with the function of anti-bacterial on this study.
Antibacterial Activity of Dayak Onion Bulbs (Eleutherine palmifolia (L) Merr) Ethanol Fraction against Pseudomonas fluorescens and Its Secondary Metabolite Analysis Immaria Fransira; Annisa Fitri Anggreini; Uun Yanuhar; Maftuch Maftuch
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.206 KB) | DOI: 10.21776/ub.rjls.2019.006.02.3

Abstract

Pseudomonas fluorescens is a gram-negative pathogenic bacteria which is one of the problems in fish farming that can causes death in fish. The alternative to overcome this bacterial attack is by using of natural material such as dayak onion bulbs (Eleutherine palmifolia (L) Merr), which are known to have secondary metabolites. The purpose of this research was to determine the antibacterial activity against P. flourescens that contained in the dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction and the content of antibacterial compounds from the best dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction. The method used in this research was antibacterial test with MIC (Minimum Inhibitory Concentration) followed by disc test and FTIR (Fourier Transform Infrared Spectroscopy) to analysis secondary metabolite. Fractionation was conducted using Chloroform: Methanol (9:1 v/v). MIC test result showed that 100 ppm was the minimum concentration that can inhibit the growth of P. fluorescens. Disc test reported that fraction 4 was the best fraction, with the dominant compounds that contained in the bulbs was phenolic compound based on FTIR test. So, the fraction of dayak onion bulbs (E. palmifolia (L) Merr) can be used as potential leads to discover new drugs.
Identification of Plankton on Fish Pond of Oreochromis niloticus Infected by Viral Nervous Necrosis Uun Yanuhar; Muhammad Musa; Dyah Tri Rahayu; Diana Arfiati
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.761 KB) | DOI: 10.21776/ub.rjls.2016.003.02.6

Abstract

The purpose of study is to determine the type of plankton found in cultivation pond of nile tilapia infected by VNN. The method is to identify the plankton in the water pond and testing the VNN that infects nile tilapia related to plankton eaten using Polymerase Chain Reaction (PCR). VNN identification results on the nile tilapia digestive tract stomach and intestines as well as plankton found in the stomach and intestines indicate VNN positively. Plankton found in the digestive tract of fish is division of Chlorophyta, Bacillariophyta, phylum Rotifera and Crustacea. It indicates that nile tilapia infected by VNN both on the gastrointestinal tract organs, stomatch or intestines and plankton were found with positive VNN digestive duct, while plankton found in water pond is VNN negative. The VNN infection depends on the nature of specifications VNN on nile tilapia and the plankton in the nile tilapia digestive duct.
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Alfarisi, Muhammad Asnin Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Baihani Amira Masitha Anasiru, Arief Farhan Kurniawan Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Anni Faridah Annisa Fitri Anggreini Apri Supii Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Bili Darnanto Susilo Binoj, Joseph Selvi BS, Sumitro Choirul Huda Choirul Huda Daimon Syukri Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Fitri, Kharisma Nuria Fitrianesia, Fika Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gunanti Mahasri Hardoko Hardoko Herlina, Cucun Herly Evanuarini Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Komarudin Komarudin MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Maria Kristiani Marsono Marsono Martiningsih, Nur Fauzi-yah Martiningsih, Nur Fauziyah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Musa Mufidah, Rhobithotus Muhamad Muhajir Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika R Adharyan Islamy Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari, Septi Setyayunita, Tamaryska Shiddiq, Muhammad Faiq Ash Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Ulhaq, Ulfieda Anwar Utari, Inpita Casuarina Eqisetia Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Arif Wahyudi Yusuf Wahyudi