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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Kedokteran Brawijaya Jurnal Ilmu dan Teknologi Hasil Ternak The Journal of Experimental Life Sciences (JELS) AGRIKA Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Mahasiswa Manajemen Sumberdaya Perairan Wacana, Jurnal Sosial dan Humaniora Research Journal of Life Science REKAYASA Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan Indonesian Journal of Natural Pigments Journal of Aquaculture and Fish Health Syntax Idea Jurnal Karinov Jurnal Abdi Insani Jurnal Pengabdian, Pendidikan dan Teknologi Teknomekanik JURNAL BAHARI PAPADAK Livestock and Animal Research Jurnal Penelitian Chanos Chanos Jurnal Kedokteran Hewan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Yanuhar, Uun
Aquatic Resources Management, Fisheries And Marine Science Faculty, Universitas Brawijaya
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Characteristics of sisal-epoxy composite boards with sodium chloride-treated fibers at different treatment temperatures Setyayunita, Tamaryska; Suryanto, Heru; Aminnudin, Aminnudin; Osman, Azlin Fazlina; Yanuhar, Uun
Teknomekanik Vol. 8 No. 1 (2025): Regular Issue
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/teknomekanik.v8i1.35572

Abstract

The growing environmental concerns associated with synthetic fibers have led to the increased adoption of bio-fibers as reinforcements in polymer composites. Sodium chloride (NaCl) is explored as a fiber treatment agent to enhance the adhesion between fibers and the matrix. The study aims to evaluate the effects of NaCl treatment on the characteristics of sisal fiber-epoxy composite boards. A completely randomized design was applied with three factors: treatment temperature (25 °C and 100 °C), NaCl concentration (1, 3, and 5 wt%), and composite board density (0.40, 0.60, and 0.80 g/cm³). Sisal fibers were soaked in NaCl solutions for one hour, rinsed, dried, and manually blended with epoxy at a ratio of 80:20 wt%. Composite board properties were observed according to the standards of JIS-A-5908, Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). Results indicated that increasing the NaCl concentration and treatment temperature significantly improved the properties of the composite board. The optimal parameters (5wt% NaCl, 100 °C, 0.80g/cm³) yielded a modulus of elasticity of 4.59±0.26 GPa, modulus of rupture of 18.88±0.03 MPa, and internal bond strength of 3.92±0.18 MPa, representing increases of 200.32%, 130.65%, and 218.70%, respectively. Thickness swelling decreased to 2.13±0.43% (48.14%) and water absorption to 8.95±0.05% (32.25%). These findings confirm that NaCl treatment is an eco-friendly method to enhance the mechanical strength and moisture resistance of biofiber composites. It also supports the development of high-performance composite boards.
THE EFFECT OF CRUDE PROTEIN HALIMEDA SP. ON CYPRINUS CARPIO INFECTED KOI HERPES VIRUS ON EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-1 Yanuhar, Uun; Wahyuningtyas, Rika
JFMR (Journal of Fisheries and Marine Research) Vol. 1 No. 1 (2017): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2017.001.01.3

Abstract

Halimeda sp. is a kind species of macroalgae that abundant grow in Indonesia. The utilization of Halimeda especially protein for disease prevention caused by the virus has not been done to the fish. The purpose of the study is to know the  treatment of crude protein Halimeda sp on  Cyprinus carpio infected by Koi Harvest Virus (KHV) on the expression of Major Histocompatibility Complex (MHC) class 1. MHC class-1 is one of the immune adaptive response on fish like this C. carpio. The methods are isolation of crude protein Halimeda sp,   purification  of  crude protein, Haemagglutination (HA) test and dot blot test. The result shows that the crude protein of Halimeda sp. can produce of adaptive immune response like MHC class 1. MHC class-1 has the function of  the immune system for maintenance of virus attacks directly. Responsible of treatment crude protein Halimeda sp. on C. carpio can be showed quantitatively using the dot blot test result. The  conclusion  is the crude protein of Halimeda sp. able to activate the MHC class I and to inhibit the proliferation of the KHV and maintenance of the fish cell of C. carpio..
N-VIVO TEST OF Chlorella vulgaris EXTRACT AS HEAT SHOCK PROTEINS INDUCTION OF CANTANG GROUPER (Ephinephelus fuscoguttatus-lanceolatus) INFECTED BY VIRAL NERVOUS NECROSIS Masitha, Amira; Yanuhar, Uun; Hertika, Asus Maizar Suryanto
JFMR (Journal of Fisheries and Marine Research) Vol. 3 No. 1 (2019): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2019.003.01.3

Abstract

Cantang Grouper fish (Ephinephelus fuscoguttatus-lanceolatus) has a high value both in international and local markets. However, these fishes have decreased production due to viral infection of VNN (Viral Nervous Necrosis). In relation to viral infections, heat shock protein (HSP) inducer derived from C. vulgaris is an effort to be used as an anti-inflammatory agent due to VNN infection. The aim of this research to know the potential of C. vulgaris extracts as an inducer of heat shock protein. The method used in this research is experimental method using in-vivo test treatment of C.vulgaris extracts with of 33 μg/ml in Cantang grouper fish with four treatments that are (A) control fish, (B) fish infected with VNN, (C) fish treated with C. vulgaris extracts, and (D) Fish treated with C. vulgaris extracts  and VNN infection. The results showed that in the phytochemical test, C. vulgaris extracts showed that there are three compounds contained are alkaloids, terpenoids, and tannins. Control fish was labelled using IgG anti Hsp 70 anti-mouse inflammatory response no apparent inflammatory reaction, fish treated with  33 μg/ml of C. vulgaris extracts HSP-70 responses was low inflammatory response, and fish treated with  33 μg/ml of C. vulgaris extract and VNN inflammatory response strengthened and tissue inflammatory response only with VNN infection were very strong. The results showed the treatment of 33 μg/ml of C. vulgaris extracts can inhibit the development of the virus also capable reduced the inflammatory response to Cantang grouper infected by VNN.
In-Vivo Test of Spirulina sp as Inducer of β-Actin In Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis Irawanto, Yovan Endik; Yanuhar, Uun; Kurniawan, Andi
JFMR (Journal of Fisheries and Marine Research) Vol. 2 No. 3 (2018): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2018.002.03.11

Abstract

Cantang grouper fish (Epinephelus fuscoguttatus-lanceolatus) is a result of hybridized fish between a female of tiger grouper fish (Epinephelus fuscoguttatus) and male of kertang grouper (Ephinephelus lanceolatus). In the development of cultivation, there are many problems, one of them infected with the class of virus Nodaviridae, namely Viral Nervous Necrosis (VNN). Fish had a defense against cellular immunity against the VNN virus by β-actin. The aims of this research to explore the crude extract of Spirulina sp as a β-actin inducer for the anti-inflammatory immune system in grouper fish against VNN attack. The method used in this research is experiment methods.  Crude extracts of Spirulina sp (33 μg/ml) were conducted by feeding orally to groupers, and VNN infections were conducted by feeding the already positive VNN meat. Detection of VNN using RT-PCR, however β-actin detection using PCR, and IHC in the organ of Cantang grouper fish. The results showed that the percentage of DAB value of control fish (14.0%), fish treated with Spirulina sp (25.7%), fish treated with VNN (31.9%), and fish treated with Spirulina sp extract and VNN infection (32.4%). The percentage of DAB values were indicated by the detection of the target gene β-actin. Immunity in fish increases with the addition of Spirulina sp. The increased β-actin expression may also be used as an indicator of a grouper's body defense against VNN infection.
Effect of Extracellular Crude Protein (ECP) on expression IL-1, IL-6, IFN- in nile tilapia (Oreochromis niloticus) Herlina, Cucun; Fadjar, Mohammad; Maftuch, Maftuch; Yanuhar, Uun
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.43338

Abstract

Modern strategies incorporate preventive measures such as extracellular products, as soluble proteins with antigenic properties, as well as generating a broad immune response. The aim of research is to determine and analyze the effect of the extracellular product of E. tarda on enhancing the immunity of Nile tilapia (O. niloticus). The research design with treatment durations of d0, 3rd, 6th, 9th day, and control (A, B, C, D, K). ECP vaccination was injected intraperitoneally at a dose of 0.1 ml/fish. The parameters analyzed are the expression of IL-1, IL-6, and IFN-, phagocytic activity, leukocyte differential, total white blood cells, and relative percent survival. The results of phagocytosis activity were highest in treatment C at 84.18% 1.81. The highest leukocyte differential in treatment C was lymphocytes at 56% 2.00, monocytes at 28% 2.00, and 16% 0.00, with a total leukocyte count of 29 x 10^3 cells 2.64, and RPS values ranging from 83.33% to 100% over 9 days of post-vaccination maintenance. Then, post-vaccination, there was an increase in the expression levels of the cytokine genes IL-1 and IL-6, but there was no increase in the expression levels of the IFN- gene compared to the control in Nile tilapia. (O. niloticus).Keywords:ECPIL-1IL-6IFN-Immune Response
Hematological Profile of Tilapia (Oreochromis niloticus) as a Bioin-dicator to Evaluate Water Quality of Floating Net Cage Cultivation System in Ranu Grati, Indonesia Alfarisi, Muhammad Asnin; Hertika, Asus Maizar Suryanto; Yanuhar, Uun
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.75605

Abstract

Ranu Grati is a volcanic lake situated in Grati District, Pasuruan Regency, East Java, Indonesia. The rapid expansion of floating net cage aquaculture in Ranu Grati has raised concerns about water quality deterioration and its impact on fish health. This study aimed to evaluate the hematological profile of tilapia (Oreochromis niloticus) as a bioindicator for assessing water quality in floating net cage cultivation systems. The purpose of this research was to examine the hematological profile of tilapia fish alongside a series of water quality parameter measures as an environmental factor influencing fish life. Sampling was conducted with three repetitions every three weeks from September to November 2024 at five distinct locations, which were deemed representative of each group of floating net cages distributed around the Ranu Grati region. The Canonical Correspondence Analysis (CCA) approach was utilized to ascertain the relationship between hematological profile of tilapia and water quality. The data analysis indicates that the water quality at Stations 2 and 3 in the research area was somewhat polluted, but Stations 1 and 5 were heavily polluted. The elevated pollution levels at Stations 1 and 5 are attributed to the significant quantity of floating net cages in the vicinity relative to other stations. The investigation indicated that low dissolved oxygen levels and elevated ammonia concentrations impacted the hematological parameters of Tilapia fish. All water quality characteristics influenced the status of leukocytes, erythrocytes, and hemoglobin at moderate quantities. Moreover, micronuclei and hematocrit levels were significantly affected by elevated concentrations of ammonia, temperature, BOD, and pH, whereas DO, TDS, and TSS influenced lower values.
The Characterization of Haemaglutinin Protein of Vili Vibrio alginolyticus as Adhesion Molecule on Epithelial Cells Receptor of Humpback Grouper (Cromileptes altivelis) Yanuhar, Uun
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.3499

Abstract

Vibriosis was a crucial problem and a primer infection causing mortality of humpback grouper.Virulence factor of vibrio, such as vili, is an adhesion molecule protein based on haemaglutination test toblood serum of humpback grouper. This research was aimed to characterize an adhesion molecule of viliprotein of V. alginolyticus on epithelial cells of Humpback grouper as haemaglutinin protein. Themethods are laboratory exploration by isolate epithelial cells of Humpback grouper, characterize a receptorand test vili protein to adhesion molecule that is epithelial cell of Humpback grouper. The result of proteinhaemaglutinin test to vili V. alginolyticus show that an adhesion molecule with a molecule weight of38.98 kDa is an adhesin vili V. alginolyticus and a haemaglutinin. The adhesion pattern of V.alginolyticus on epithelial cells of Humpback grouper exposed to protein haemaglutinin of vili show bothdiffuse and localize pattern. Research conclusion was the haemaglutinin protein of vili V. alginolyticuswas an adhesin having a role to both virulence process of bacteria and attachment process to receptor ofepithelial cells of Humpback grouper.Keywords: adhesion molecule, epithelial cell, vili, V. alginolyticus, C. altivelis
Immunological Response of Interleukin-6 on Cantang Hybrid Grouper (Ephinephelus sp.) By Induction Of Protein Brachionus sp. Kumalaningrum, Dwi Retna; Yanuhar, Uun; Musa, Mohammad; Martiningsih, Nur Fauzi-yah
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.30835

Abstract

This study examines the role of Brachionus sp protein on the interleukin-6 pro-inflammatory immune system in Cantang grouper infected with Viral Nervous Necrosis (VNN). The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of Interleukin-6 as an indicator of increased fish body defense system against VNN. Testing of Brachionus protein by injection at doses of 35 µl, 105 µl, and 170 µl /150 gram Cantang grouper. The results showed that the lowest decrease was at a dose of 105 µl/150-gram Cantang groupers.  In addition, Brachionus sp. contains 3 protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa. 
KARAKTERISTIK SEDIMEN DI PERAIRAN PESISIR LEKOK, KABUPATEN PASURUAN, JAWA TIMUR Anggraini, Rifka Rimbi; Yanuhar, Uun; Risjani, Yenny
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 12 No. 1 (2020): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.681 KB) | DOI: 10.29244/jitkt.v12i1.28705

Abstract

Sedimentasi merupakan salah satu dinamika yang terjadi di kawasan pesisir untuk mengetahui kondisi pantai tersebut. Pesisir perairan Lekok merupakan salah satu kawasan perairan terbuka yang memiliki dinamika perubahan pesisir sangat fluktuatif. Studi sebaran jenis sedimen yang dilakukan secara terstruktur pada lokasi ini digunakan untuk memberikan informasi mengenai proses transportasi dan pengendapan sedimen berdasarkan distribusi ukuran butir sedimen. Penelitian ini bertujuan untuk mengetahui ukuran butir sedimen secara horizontal di pesisir perairan Lekok. Penelitian ini dilakukan pada sembilan titik sampling dengan pengambilan sampel berupa sedimen dasar secara acak dengan menggunakan metode grab sampler. Selanjutnya dilakukan analisis lebih lanjut dengan metode gravimetri. Berdasarkan hasil penelitian diketahui bahwa karakteristik sedimen dalam Pesisir perairan Lekok umumnya didominasi oleh tiga jenis sedimen yaitu: lempung berpasir, lempung dan lempung lanau. Nilai sorting berada dalam kondisi well-sorted hingga kondisi moderately well-sorted di mana kondisi sedimen didominasi dengan well sorted. Nilai skewness berada pada nilai skewed positively dimana nilai menunjukkan hasil positif. Nilai kurtosis berada pada leptokurtic dimana sedimen dari stasiun 1 ke stasiun 9 homogen.
Kajian Cemaran Mikroplastik Terhadap Profil Darah Ikan Bandeng (Chanos chanos) di Perairan Utara Jawa Timur Uun Yanuhar; Andik Isdianto; Defa Rizqi Machfuda; Gilang Rusrita Aida; Lutfia Fahimatul Ilmi; Nezya Pramudya Wardani; Nico Rahman Caesar
Samakia : Jurnal Ilmu Perikanan Vol 16 No 1 (2025): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35316/jsapi.v16i1.6058

Abstract

Pencemaran mikroplastik di ekosistem perairan telah menjadi perhatian global karena dampaknya terhadap biota laut, termasuk ikan yang merupakan sumber pangan utama bagi manusia. Penelitian ini bertujuan untuk mengkaji pengaruh cemaran mikroplastik terhadap profil darah ikan bandeng (Chanos chanos) di perairan utara Jawa Timur. Sampel ikan bandeng diambil dari beberapa lokasi lokasi yang memiliki tingkat pencemaran mikroplastik berbeda. Analisis mikroplastik dalam jaringan ikan dilakukan menggunakan metode mikroskopis dan spektroskopi, sementara analisis profil darah mencakup pemeriksaan parameter hematologis seperti jumlah eritrosit, leukosit, kadar hemoglobin, dan hematokrit. Hasil penelitian menunjukkan adanya akumulasi mikroplastik dalam jaringan ikan bandeng di seluruh lokasi pengambilan sampel, dengan konsentrasi tertinggi ditemukan di area dengan aktivitas industri dan pemukiman padat. Pengaruh mikroplastik terhadap profil darah ikan terlihat dari peningkatan jumlah leukosit dan penurunan kadar hemoglobin yang signifikan pada ikan yang terpapar mikroplastik dalam jumlah tinggi. Hal ini mengindikasikan adanya respon stres dan inflamasi akibat paparan mikroplastik, yang berpotensi memengaruhi kesehatan ikan dan kemampuan adaptasinya terhadap kondisi lingkungan. Penelitian ini menunjukkan bahwa cemaran mikroplastik dapat berdampak negatif pada sistem hematologi ikan bandeng, yang berimplikasi pada kesehatan ekosistem dan keamanan pangan bagi manusia. Temuan ini mendukung perlunya tindakan mitigasi pencemaran mikroplastik di perairan pesisir serta pengembangan kebijakan pengelolaan lingkungan yang lebih efektif.
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Agus Susilo Alfarisi, Muhammad Asnin Alfi Nur Rusydi Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Masitha Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Annisa Fitri Anggreini Apri Supii Arief Farhan Kurniawan Anasiru Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Baihani, Amira Bili Darnanto Susilo BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Defa Rizqi Machfuda Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Ekwan Nofa Wiratno Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gilang Rusrita Aida Gilang Rusrita Aida Gunanti Mahasri Hardoko Hardoko Hartati Kartikaningsih Herlina, Cucun Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Islamy, R Adharyan Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Kharisma Nuria Fitri Kristiani, Maria Lutfi Ni`'Matus Salamah Lutfia Fahimatul Ilmi MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marsono Marsono Martiningsih, Nur Fauzi-yah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Fadjar Mohammad Musa Mohammad Musa Mufidah, Rhobithotus Muhajir, Muhamad Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nezya Pramudya Wardani Nezya Pramudya Wardani Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari Septi Anitasari, Septi Setyayunita, Tamaryska Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Umi Zakiyah Utari, Inpita Casuarina Eqisetia Wahyudi, Yusuf Arif Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Wahyudi Zainal Abidin