Articles
Identification of Koi Herpesvirus on Koi Fish (Cyprinus carpio) with Immunocytochemistry Test Streptavidin Biotin
Rika Wahyuningtyas;
Uun Yanuhar
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (321.292 KB)
|
DOI: 10.21776/ub.rjls.2018.005.03.2
Koi herpesvirus (KHV) has been identified in common carp (Cyprinus carpio) and varieties of C. carpio such as koi carp. Handling KHV outbreak on koi fish are always late and on case KHV infection, hence the purpose of this study is to find an alternative method in identifying a particular fish diseases KHV which more simple, practical and accurate. 20 samples are positively infected by KHV were derived from Blitar, with the characteristic of them are gill slime, erosion or ulcers on the skin, movement were not controlled, and skin blister. The result of our study showed that 100% positive reaction to the ICC in smear blood, which is marked golden chocolate color. The result of immunostaining streptavidin biotin obtained that koi fish have been infected by severe KHV showed golden chocolate color on the blood smear compared to positive control. While fish have been infected by undemanding KHV infection or still in the beginning stage, the blood smear showed chocolate bluish. It can be concluded that Immunocytochemistry Test Streptavidin Biotin can be used for early identifying of Koi Herpes Virus (KHV) due to simpler producer, more practical, and accurate result.
Identifikasi Ikan Genus Mystus Dengan Pendekatan Genetik
Taufik Budhi Pramono;
Diana Arfiati Arfiati;
Maheno Sri Widodo;
Uun Yanuhar Yanuhar
Jurnal Sumberdaya Akuatik Indopasifik Vol 1 No 2 (2017): November
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (720.956 KB)
|
DOI: 10.30862/jsai-fpik-unipa.2017.Vol.1.No.2.34
The fish of the Bagridae Family in Indonesia reach until 60 species and one of them is from the genus Msytus. The method is used for fast and accurate species identification was needed. DNA barcoding was a new identification method with molecularly approach. The CO1 gene was amplified and PCR products were sequenced and analyzed using bioinformatics software. Editing of sequencing results and determining the nucleotide composition were analyzed with Mega5 software. The DNA sequence was aligned with ClustalW vers. 1.4. Sequences are compared with GenBank data using BLAST (Basic Local Aligment Search Tools) and BOLDSystems. Phylogenetic tree was made using Neighbor_Joining method. One example of the identification of fish from the confirmed Mystus genus is Mystus nigriceps being the Mystus singaringan.
PELATIHAN DAN PENDAMPINGAN PENGELOLAAN PAKAN MANDIRI DAN LINGKUNGAN BERKELANJUTAN GUNA MENINGKATKAN BUDIDAYA IKAN LELE DI KABUPATEN TUBAN
Uun Yanuhar;
Dyah Kinasih Wuragil
Jurnal Pengabdian Pendidikan dan Teknologi (JP2T) Vol 2, No 1 (2021)
Publisher : Universitas Negeri Malang
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.17977/um080v2i12021p30-36
Ikan lele (Clarias sp) adalah salah satu komoditas ikan air tawar yang memiliki peminat yang tinggi. Hal ini menyebabkan para pembudidaya berusaha untuk meningkatkan usaha budidaya ikan lele. Kabupaten Tuban adalah salah satu lokasi kawasan perikanan budidaya ikan lele di Jawa Timur. Program Pengabdian Masyarakat ini bertujuan memberdayakan para petani ikan untuk memproduksi pakan mandiri, sebagai bentuk pengembangan usaha pembesaran ikan lele dalam memecahkan masalah semakin meningkatnya harga pakan ikan. Keberadaan unit produksi pakan mandiri sifatnya yang tidak memakan lahan yang luas kemudian pelaksanaan teknisnya yang praktis dan merupakan kegiatan perikanan yang berprospek ekonomis sehingga menjadi alasan untuk menjadi bagian dari kegiatan pemberdayaan masyarakat yang berbasis perikanan di Desa Campurejo, Desa Jenggolo dan Desa Tegalagung. Penerapan IPTEK mengenai pembuatan/produksi pakan secara mandiri baik berupa alat pembuat pakan dan formulasi bahan pakan itu sendiri sehingga dapat meningkatkan pengembangan usaha budidaya ikan lele yang ada di Kabupaten Tuban. Melalui program ini para anggota mitra mendapatkan pembekalan materi edukasi dalam melakukan kegiatan berbudidaya ikan lele agar di masa depan dapat mengatasi permasalahan yang terjadi dalam budidaya ikan lele sehingga produksi ikan lele meningkat dan kerugian berkurang, sehingga perekonomian anggota mitra meningkat. Berdasarkan hasil kegiatan menunjukkan bahwa target dari kegiatan Pengabdian kepada masyarakat skema Program Kemitraan Masyarakat (PKM) terpenuhi dengan adanya peningkatan ketrampilan dan pengetahuan dalam berbudidaya ikan lele yang semakin baik, peningkatan pemahaman terhadap monitoring kualitas air kolam, selain itu juga peningkatan pemahaman terhadap penyakit yang dialami ikan lele oleh para anggota mitra.Kata kunci – Ikan Lele (Clarias sp); Pakan Mandiri; Pemberdayaan Masyarakat; Manajemen Lingkungan.
PELATIHAN PEMELIHARAAN IKAN KOI UNTUK PENGEMBANGAN WISATA IKAN DI KAWASAN BEDENGAN, SELOREJO, MALANG
Heru Suryanto;
Bili Darnanto Susilo;
Aminnudin Aminnudin;
Sukarni Sukarni;
Suprayitno Suprayitno;
Marsono Marsono;
Uun Yanuhar
Jurnal Pengabdian Pendidikan dan Teknologi (JP2T) Vol 2, No 1 (2021)
Publisher : Universitas Negeri Malang
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.17977/um080v2i12021p14-22
Wana wisata Bedengan berada di Dusun Selokerto, Desa Selorejo, Kecamatan Dau, Kabupaten Malang. Wisata ini telah dibuka sejak tahun 2007 pada lahan seluas 13,7 hektar yang terletak ±12 km dari Universitas Negeri Malang. Tempat ini memiliki udara khas dataran tinggi, ditumbuhi oleh pepohonan pinus, tanah yang masih subur dan terdapat aliran sungai dengan airnya yang sangat jernih sehingga tempat ini sangat cocok sebagai wisata edukasi tentang ekosistem. Mitra desa ingin mengembangkan wisata edukasi ikan di tempat ini, sehingga sangat diperlukan pengetahuan, keterampilan dan teknik budidaya ikan. Untuk itu dilakukan beberapa tahapan kegiatan, yaitu: 1. Survey lokasi wana wisata bedengan; 2. Pengkondisian lokasi yang pas untuk dijadikan kolam ikan dan pengambilan sampel air; 3. Pembelian dan pengkondisan ikan koi; 4. Pemindahan ikan koi ke kolam; 4. Pelatihan pemeliharaan ikan yang melibatkan 11 anggota pengelola wisata bedengan serta mengundang narasumber ahli. 5. Pemberian bantuan pakan serta praktek pemeliharaan ikan koi. Setelah pelaksaan rangkaian kegiatan tersebut, anggota pengelola wisata bedengan sudah dapat memahami dan mampu melakukan proses pemeliharaan ikan koi dengan baik. Para pengunjung juga memberikan respon positif dengan adanya wisata edukasi ikan ini.
N-VIVO TEST OF Chlorella vulgaris EXTRACT AS HEAT SHOCK PROTEINS INDUCTION OF CANTANG GROUPER (Ephinephelus fuscoguttatus-lanceolatus) INFECTED BY VIRAL NERVOUS NECROSIS
Amira Masitha;
Uun Yanuhar;
Asus Maizar Suryanto Hertika
JFMR (Journal of Fisheries and Marine Research) Vol 3, No 1 (2019): JFMR VOL 3 NO.1
Publisher : JFMR (Journal of Fisheries and Marine Research)
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (363.14 KB)
|
DOI: 10.21776/ub.jfmr.2019.003.01.3
Cantang Grouper fish (Ephinephelus fuscoguttatus-lanceolatus) has a high value both in international and local markets. However, these fishes have decreased production due to viral infection of VNN (Viral Nervous Necrosis). In relation to viral infections, heat shock protein (HSP) inducer derived from C. vulgaris is an effort to be used as an anti-inflammatory agent due to VNN infection. The aim of this research to know the potential of C. vulgaris extracts as an inducer of heat shock protein. The method used in this research is experimental method using in-vivo test treatment of C.vulgaris extracts with of 33 μg/ml in Cantang grouper fish with four treatments that are (A) control fish, (B) fish infected with VNN, (C) fish treated with C. vulgaris extracts, and (D) Fish treated with C. vulgaris extracts and VNN infection. The results showed that in the phytochemical test, C. vulgaris extracts showed that there are three compounds contained are alkaloids, terpenoids, and tannins. Control fish was labelled using IgG anti Hsp 70 anti-mouse inflammatory response no apparent inflammatory reaction, fish treated with 33 μg/ml of C. vulgaris extracts HSP-70 responses was low inflammatory response, and fish treated with 33 μg/ml of C. vulgaris extract and VNN inflammatory response strengthened and tissue inflammatory response only with VNN infection were very strong. The results showed the treatment of 33 μg/ml of C. vulgaris extracts can inhibit the development of the virus also capable reduced the inflammatory response to Cantang grouper infected by VNN.
In-Vivo Test of Spirulina sp as Inducer of β-Actin In Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis
Yovan Endik Irawanto;
Uun Yanuhar;
Andi Kurniawan
JFMR (Journal of Fisheries and Marine Research) Vol 2, No 3 (2018): JFMR VOL 2 NO 3
Publisher : JFMR (Journal of Fisheries and Marine Research)
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (517.566 KB)
|
DOI: 10.21776/ub.jfmr.2018.002.03.11
Cantang grouper fish (Epinephelus fuscoguttatus-lanceolatus) is a result of hybridized fish between a female of tiger grouper fish (Epinephelus fuscoguttatus) and male of kertang grouper (Ephinephelus lanceolatus). In the development of cultivation, there are many problems, one of them infected with the class of virus Nodaviridae, namely Viral Nervous Necrosis (VNN). Fish had a defense against cellular immunity against the VNN virus by β-actin. The aims of this research to explore the crude extract of Spirulina sp as a β-actin inducer for the anti-inflammatory immune system in grouper fish against VNN attack. The method used in this research is experiment methods. Crude extracts of Spirulina sp (33 μg/ml) were conducted by feeding orally to groupers, and VNN infections were conducted by feeding the already positive VNN meat. Detection of VNN using RT-PCR, however β-actin detection using PCR, and IHC in the organ of Cantang grouper fish. The results showed that the percentage of DAB value of control fish (14.0%), fish treated with Spirulina sp (25.7%), fish treated with VNN (31.9%), and fish treated with Spirulina sp extract and VNN infection (32.4%). The percentage of DAB values were indicated by the detection of the target gene β-actin. Immunity in fish increases with the addition of Spirulina sp. The increased β-actin expression may also be used as an indicator of a grouper's body defense against VNN infection.
PENINGKATAN POTENSI KELOMPOK MASYARAKAT MELALUI PENERAPAN TEKNOLOGI MICROBUBBLE DAN INTERNET OF THINGS DI POKDAKAN RUKUN ABADI MAKMUR
Uun Yanuhar;
Muhammad Musa;
Herly Evanuarini;
Dyah Wuragil;
Fajar Shodiq;
Nur Junirahma;
Nico Rahman Caesar
Jurnal Pengabdian Pendidikan dan Teknologi (JP2T) Vol 3, No 1 (2022)
Publisher : Universitas Negeri Malang
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.17977/um080v3i12022p27-32
Seiring berkembangnya sektor budidaya ikan koi, banyak ditemukan permsalahan dalam prosesnya. Berbagai macam permasalahan tersebut yaitu penyakit, penurunan daya dukung air dan kendala pemasaran ditengah masa pandemi Covid 19 ini. Guna mengatasi permasalahan yang maka diperlukan penerapan teknologi untuk meningkatkan potensi budidaya ikan koi. Penerapan teknologi yang diterapkan harus mudah dipahami dan berkelanjutan sehingga efektif dan efisien dalam penerapannya. Tujuan Pengabdian Kepada Masyarakat Doktor Mengabdi ini adalah transfer paket teknologi Microbubble dan Internet of Things (IoT) pada kolam budidaya ikan koi untuk meningkatkan potensi kelompok masyarakat. Selain itu, juga dilakukan pelatihan dan pendampingan terkait penerapan paket Microbubble dan Internet of Things (IoT). Metode pelaksanaan kegiatan Pengabdian Kepada Masyarakat Doktor Mengabdi meliputi, memberikan pelatihan dan pendampingan mengenai penerapan ilmu pengetahuan dan teknologi (IPTEK) dengan menggunakan Microbubble berbasis Internet of Things (IoT) dalam mendukung budidaya ikan dengan sistem intensif dan mengatasi permasalahan fluktuasi kualitas air yang selama menjadi kendala. Teknologi Microbubble berbasis Internet of Things (IoT) yang diterapkan telah disesuaikan dengan kebutuhan kolam, tipe kolam serta memudahkan dalam pemeliharaannya. Pelaksanaan kegiatan diikuti oleh seluruh anggota tim pengabdian dan anggota mitra kegiatan. Berdasarkan hasil pelatihan dan pendampingan yang telah dilakukan terdapat peningkatan Sumber Daya Manusia, dilihat dari meningkatnya pengetahuan dan pemahaman anggota mitra dalam penerapan paket teknologi yang diberikan dan mendukung peningkatan produksi budidaya ikan koi yang berkelanjutan
PEMBERDAYAAN MASYARAKAT MELALUI PENERAPAN TEKNOLOGI MICROBUBBLE TERKONTROL IOT PADA KOLAM IKAN LELE DI POKDAKAN ROI LELE KABUPATEN MALANG
Heru Suryanto;
Aminnudin Aminnudin;
Uun Yanuhar
Jurnal Pengabdian Pendidikan dan Teknologi (JP2T) Vol 3, No 1 (2022)
Publisher : Universitas Negeri Malang
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.17977/um080v3i12022p1-7
Ikan lele merupakan salah satu ikan budidaya yang banya digemari oleh masyarakat Jawa Timur sehingga permintaannya setiap tahun semakin meningkat. Seiring meningkatnya permintaan yang meningkat tidak diimbangi dengan pengelolaan kualitas air yang baik. Salah satu penyebabnya karena kurangnya pemahaman petani ikan akan pentingnya penerapan teknologi dalam proses budidaya ikan. Oleh karena itu, perlu adanya pemberdayaan masyarkaat melalui Pengabdian kepada masyarakat mengenai penerapan teknologi dalam budidaya ikan. Program Pengabdian Kepada Masyarakat ini akan berfokus pada satu mitra yang saat ini tengah mengembangkan usaha perikanan budidaya lele, yaitu Kelompok Budidaya Ikan (Pokdakan) ROI LELE. Pokdakan ROI LELE berada di desa Bringin, kecamatan Wajak, Kabupaten Malang, Provinsi Jawa Timur. Metode pelaksanaan PKM ini meliputi memberikan pelatihan dan pendampingan mengenai penerapan teknologi Microbubble Generator (MBG) yang terkoneksi dengan Internet of things (IOT). Sistem MBG ini dilengkapi sensor untuk sistem monitoring kadar oksigen terlarut dan pH dengan mengintegrasikan konsep IOT untuk memonitor dan mengendalikan MBG secara otomatis maupun manual melalui kontrol smartphone. Berdasarkan hasil penerapan MBG dan IOT pada kolam ikan lele didapatkan hasil kadar oksigen terlarut meningkat dan menjaga pH perairan tetap stabil sehingga mendukung kelangsungan pertumbuhan ikan lele. Selanjutnya, capaian dari PKM ini ialah adanya peningkatan pengetahuan dan keterampilan petani ikan serta peningkatan kapasitas produksi ikan lele dengan adanya penerapan MBG dan IOT.
Effect of Homogenization Pressure on Bacterial Cellulose Membrane Characteristic Made from Pineapple Peel Waste
Muhamad Muhajir;
Heru Suryanto;
Yanuar Rohmat Aji Pradana;
Uun Yanuhar
Journal of Mechanical Engineering Science and Technology (JMEST) Vol 6, No 1 (2022)
Publisher : Universitas Negeri Malang
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.17977/um016v6i12022p034
Many studies were conducted to maintain the environment by reducing the waste, especially pineapple peel waste. This study aims to explore the effect of various pressure of the homogenization process on bacterial cellulose membrane surface morphology and structure produced using extract of pineapple peel waste. The methods include the preparation of pellicle samples from the product of the fermentation process of Acetobacter xylinum using a medium from the extract of pineapple peel waste. Bacterial cellulose pellicles were crushed using a blender. Mashed bacterial cellulose pellicle then homogenized in High-Pressure Homogenizer with pressure variation of 0 bar, 150 bar, 300 bar, 450 bar, dan 600 bar then cast into a mold. The bacterial cellulose solutions were dried in an oven at 60°C for 8 hours. The dried bacterial cellulose membrane was analyzed using XRD for the structure and SEM analysis for the morphology. The results indicate that the crystalline properties of BCM were shifted after being treated by various pressure processing in a High-Pressure Homogenizer. It was found that the High-Pressure Homogenizer with higher pressure reduced the peak intensity, decreased crystalline index from 87% to 70%, and decreased the degree of crystalline from 88% to 77% without changing the cellulose structure. The higher pressure of the homogenization process causes the porosity of the membrane to be decreased.
Expression of Hsp70 and β-actin Genes as The Immune Response against Viral Nervous Necrosis that Infected Asian Seabass (Lates calcalifer)
Yusuf Arif Wahyudi;
Uun Yanuhar;
Maftuch Maftuch
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1115.084 KB)
|
DOI: 10.21776/ub.jels.2018.008.03.02
The viral nervous necrosis (VNN) is betanodavirus of the family Nodaviridae with acute infection and associated with high levels mortality up to 100% of numerous larval-stage marine and freshwater fish species. The danger signals of viral infection that are capable of activating APCs (Antigen Presenting Cells), furthermore produced molecules protein receptors such as interferon and heat shock proteins. Stimulating interferon type I (IFN I) induce several antiviral molecules, further binding the actin cytoskeleton to reach the site of infection. This study demonstrated a correlation between increased levels of Hsp70 (heat shock proteins) and actin filamentous (β-actin) within invasion wild-type isolate of ssRNA VNN from Asian Seabass (L. calcalifer) juvenile. Furthermore, expression of Hsp70 and actin as an indicator or biomarker of stressed states in fish. The reverse transcriptase polymerase chain reaction (RT-PCR) method used to finding expression Hsp70 and β-actin. Whereas nested RT-PCR used for VNN genes. The up-regulation of Hsp70 was observed on the brain tissue higher than eye tissue of sample positive infected VNN. Whereas different expression of β-actin, β-actin receptor expression tends to be stable in the organs of the brain and eye, both invaded VNN or normal, while on the eye, the ratio slightly increased based on the intensity of the band. The research shows that altered expression of heat shock protein 70 and beta-actin gene receptors in the target organs that response to invasion VNN at Asian seabass (L. calcalifer) juvenile. Alteration expression of heat shock protein 70 and beta-actin can be used as an indicator or biomarker of stress cells, especially from VNN invasion at fish. Keywords: Asian seabass, β-actin, Hsp70, Immune response, Viral nervous necrosis.