Claim Missing Document
Check
Articles

Pengaruh Pemberian Ekstrak Etanol Kulit Buah Jeruk Baby (Citrus sinensis L. Osbeck) Selama 28 hari terhadap Kadar SGOT dan SGPT Sofia, Vivi; Widyaningsih, Wahyu; Yuliani, Sapto; Bachri, Moch Saiful
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.71513

Abstract

One of the plants used as natural medicine is baby orange (Citrus sinensis L. Osbeck). Baby oranges contain hesperidin, hesperetin, narirutin and nobiletin compounds. The purpose of this study was to determine the effect of giving ethanol extract of baby orange peel for 28 days on liver function as seen from the increase in SGOT and SGPT levels. This research is a pure experimental study with a completely randomized design (CRD) with a unidirectional pattern, namely the method used to observe the relationship of independent variables (including: normal control, negative control, ethanol extract of baby orange peel (EEKBJB) doses of 50, 100, 200 and 400 mg/kg BW) with the dependent variable (SGOT and SGPT levels). The test animals used in this study were 30 male Wistar rats which were divided into 6 treatment groups which were treated for 28 consecutive days. On day 29, blood samples were taken from the orbital sinus of the eye and the levels of SGOT and SGPT were measured. There was a significant difference in the levels of SGOT and SGPT as indicated by the results of the One Way ANOVA test (p<0.05). The results of the Post Hoc-Tukey test for SGOT levels showed a significant difference between the control group and the 50 mg/kg BW dose group, while the 100, 200 and 400 mg/kg BW doses did not show a significant difference. The results of the Post Hoc-Tukey, s-HSD test for SGPT levels did not show a significant difference between the control group and the 50, 100, 200 and 400 mg/kg BW dose groups. Significant differences were shown by doses of 50 and 400 mg/kg BW. Giving EEKBJB for 28 days did not cause toxic effects on liver function, seen from the absence of an increase in SGOT and SGPT levels.
Edukasi Kehalalan dan Keamanan Produk Berbasis Literasi Komunikasi Digital di Gilangharjo Pandak Bantul Azali, Aulia Syafadilla; Nuh, Muhammad; Rahmawati, Dhea; Saraswati, Yasti Rizkandar; Yuliani, Sapto; Sari, Eka Anisa
Jurnal SOLMA Vol. 14 No. 3 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i3.20962

Abstract

Pendahuluan: Perkembangan teknologi digital membawa dampak besar pada penyebaran informasi, termasuk terkait kehalalan dan keamanan produk. Namun, literasi masyarakat, khususnya pelaku usaha mikro, kecil, dan menengah (UMKM), masih terbatas dalam memahami standar halal-thayyib dan keamanan pangan. Studi ini bertujuan untuk meningkatkan pemahaman masyarakat Desa Gilangharjo, Bantul melalui edukasi berbasis literasi komunikasi digital. Metode: Sosialisasi, pelatihan interaktif, simulasi penggunaan laman halal resmi, pendampingan, serta evaluasi. Hasil: Adanya peningkatan signifikan pengetahuan masyarakat dengan skor rata-rata pretest 41,53 meningkat menjadi 45,69 pada postest (p=0,000). Edukasi ini memberikan dampak positif yang merata tanpa dipengaruhi faktor usia maupun jenis kelamin. Kesimpulan: Edukasi kehalalan dan keamanan produk berbasis literasi digital efektif meningkatkan kesadaran masyarakat, mendorong perilaku konsumsi kritis, serta memperkuat kesiapan UMKM menghadapi sertifikasi halal nasional.
Analysis of Characteristics, Fatty Acid Composition, Antioxidant Activity, and Beta-sitosterol of Moringa (Moringa oleifera Lam) Oil from Three Different Regions in Indonesia Chasanah, Uswatun; Yuliani, Sapto; Sugihartini, Nining
Jurnal Kefarmasian Indonesia VOLUME 15, NUMBER 2, JULY-DECEMBER 2025
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Moringa oil offers a range of health, nutritional, and cosmetic benefits. However, its characteristics can vary greatly depending on its geographical origin. This study aimed to compare the physicochemical properties, fatty acid composition, antioxidant activity, and beta-sitosterol content of moringa oil obtained from three regions in Indonesia: Central Java, Central Sulawesi, and East Nusa Tenggara. The physicochemical properties examined include organoleptic, specific gravity, viscosity, refractive index, iodine value, acid value, saponification value, and peroxide value. Furthermore, the fatty acid composition was analyzed using gas chromatography with flame ionization detection (GC-FID), antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and beta-sitosterol content was measured by thin-layer chromatography (TLC). Physical attributes, such as viscosity and specific gravity, do not exhibit significant differences between the regions. At the same time, chemical parameters, which include iodine value, acid value, and saponification value, show considerable variations. Fatty acid profiles revealed that oils from Central Sulawesi and East Nusa Tenggara have high oleic acid and low polyunsaturated fat content, providing better oxidative stability and longer shelf life. In contrast, Central Java oil showed higher levels of linoleic and behenic acids, making it more suitable for sensitive skin and hair care applications. Central Java oil exhibits the highest antioxidant activity, whereas Central Sulawesi oil contains the highest concentration of beta-sitosterol, suggesting a more significant potential as an anti-inflammatory agent. These findings highlight the influence of regional origin on the quality of moringa oil, presenting exciting opportunities for further research in this field.
Ethnopharmacology, LC-MS profiling, and molecular docking of diarrheal-healing plants by the Osing tribe Cendekiawan, Khrisna Agung; Kintoko; Yuliani, Sapto; Wardhani, Firdha Aprillia
Pharmaciana Vol. 15 No. 3 (2025): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v15i3.31315

Abstract

Indonesia has a long history of using medicinal plants, but much of this knowledge, especially among groups like the Osing people in East Java, is not well-documented. This study looks at the plants used by the Osing community to treat diarrhea. This research was conducted through interviews with residents of Banjar and Licin Villages in Banyuwangi Regency. The significance and perceived efficacy of each medicinal plant were evaluated using the ethnobotanical indices Use Value (UV) and Fidelity Level (FL). They also did tests to find the active chemicals in the plants. The most common plants for treating diarrhea were Psidium guajava (UV = 2.44) and Piper betle (UV = 1.11). Psidium guajava had the highest FL value (64.7%). Tests have shown that these plants contain essential chemicals. Further analysis confirmed the presence of quercetin and kaempferol, which are known to help alleviate stomach problems. To support these findings, computer-based tests were done, including Lipinski rule prediction, ADMET profiling, and molecular docking. The results showed quercetin binds well (ΔG = -9.72) and has a good RMSD value (1.83 Å). This suggests quercetin could be a good starting point for making antidiarrheal drugs. Overall, the study underscores the significance of traditional knowledge and recommends further research on plant-based treatments for stomach issues.
In Silico Study of Compounds Bioactive Guava (Psidium guajava) as Antidiarrheal Assisted Liquid Chromatography-Mass Spectroscopy Cendekiawan, Khrisna Agung; Kintoko, Kintoko; Yuliani, Sapto; Anggraeni, Linda Suci; Ningrum, Lailatul Fitria; Hasana, Siti Nur; Wardhani, Firdha Aprillia
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 2 (2025): Jurnal Mandala Pharmacon Indonesia 
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i2.876

Abstract

Guava leaves seeds (Psidium guajava) are known contain various compound potential bioactives? as antidiarrheal, such as quercetin and tannins, which work with method hinder intestinal contractions and disrupts permeability membrane cell bacteria. Research This aim for explore potential antidiarrheal from compound in leaf guava seed through identification compound using LC-MS and simulation molecular docking to six human protein target receptors, namely Cytochrome P450 1A1, Ephrin Type-B Receptor 4, Multidrug Resistance Protein 1, Dihydrofolate Reductase, Extracellular Calcium-Sensing Receptor, and Rap Guanine Nucleotide Exchange Factor 4. Extraction done with method maceration use solvent methanol, then compound analyzed using Liquid Chromatography–Mass Spectrometry (LC-MS). A total of eleven compound main chosen foranalyzed in silico using device soft AutoDockTools, PyRx, and Discovery Studio. Validation was performed through redocking of native ligands with RMSD value ? 2 Å. The results show some ligands such as Kaempferol, Cathecin, and Quercetin own affinity strong bond, with? Kaempferol show lowest binding energy of -9.72 kcal /mol against BHF receptor. Drug-likeness test based on Lipinski's rule also shows that part big compound fullfil criteria compound drug analysis? interaction amino acids show existence bond hydrogen and hydrophobic support? stability bond. This result show that compound leaf guava seeds, especially Kaempferol and Cathecin, potentially as candidate agent antidiarrheal natural. Further studies in vitro and in vivo are recommended for support findings.
The Description of Knowledge and Attitude of Coffee Lovers on Diabetes Melitus At Coffee Shops In Palaran District: The Description of Knowledge and Attitude of Coffee Lovers on Diabetes Melitus At Coffee Shops In Palaran District Muhammad Ma'ruf; Arif Budi Setianto; Rindi Ibrahim Lasanudin; Sapto Yuliani
KESMAS UWIGAMA: Jurnal Kesehatan Masyarakat Vol 9 No 2 (2023): July-December
Publisher : Universitas Widya Gama Mahakam Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24903/kujkm.v9i2.2224

Abstract

Background: Diabetes mellitus is a disease that is often called "the silent killer." This disorder occurs in the metabolism of carbohydrates and fats that are relatively deficient in insulin in the body. Previous research said that coffee could be used as a therapy for diabetes mellitus. The background for writing this article is the high prevalence of knowledge and attitude of coffee lovers and scientific evidence of coffee content as a decrease in blood glucose levels. Objectives: This study aims to describe the knowledge and attitude of coffee lovers toward diabetes mellitus in coffee shops in Palaran District. Research Metodes: This research method uses quantitative and descriptive. Data were obtained by distributing questionnaires to research subjects, namely coffee lovers who were in coffee shops in the Palaran District, Samarinda City, totaling 46 respondents. Results: The results showed that there were 26 coffee lovers (56.5%) who had good knowledge of diabetes mellitus and 20 people (43.5%) who had a good understanding of diabetes mellitus. Meanwhile, in the attitude of coffee lovers, there were 35 people (76.1%) who had good attitude towards diabetes mellitus and 11 people (23.9%) who had a reasonably good attitude towards diabetes mellitus. Conclusion: It can be concluded that the coffee lovers in the Palaran District coffee shop, on average, have good knowledge and attitude towards diabetes mellitus.
Effect of Avocado Leaf Ethanol Extract Gel (Persea americana Mill) on Healing Diabetic Wounds Widyaningsih, Wahyu; Yuliani, Sapto; Anasyua, Fairuz Khairunnisa; Bahagia, Tasya; Yuliawanti, Risma
Journal of Food and Pharmaceutical Sciences Vol 14, No 2 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.25562

Abstract

Diabetic wounds are a major clinical challenge due to delayed healing and increased complication risks. Ethanol extract of avocado leaves (Persea americana Mill.) has previously shown promise in enhancing tissue regeneration in diabetic wounds, but its potential in gel form remains untested. This study aimed to develop an avocado leaf ethanol extract-based gel and evaluate its woundhealing potential in diabetic mice. Avocado leaves were extracted using 70% ethanol and formulated into a gel, which was evaluated for physical properties and topical suitability. Diabetic mice were divided into five groups: normal control, negative control (gel base), positive control (bioplacenton), and two treatment groups (5% and 10% gel). Diabetes was induced using alloxan (150 mg/kg BW), followed by wound induction and treatment. Wound healing was assessed through visual assessment, wound diameter reduction, and healing duration. Statistical analysis was performed using one-way ANOVA with a 95% confidence level. Results showed the gel meets physical properties (pH test, organoleptic test, homogeneity test, spreadability test) and significantly improved wound healing in diabetic mice. The 5% gel demonstrated wound-healing activity due to bioactive compounds such as saponins, tannins, alkaloids, and flavonoids. Further analysis is needed to identify the specific components responsible for the healing effects, highlighting the gel’s potential for diabetic wound treatment.
Analisis Kualitatif dan Kuantitatif Kandungan Senyawa Flavonoid dan Fenolik pada Ekstrak Etanol Daun Mangga Kasturi (Mangifera Casturi Kosterm.) Rahmayani Maghfirah; Mochammad Saiful Bachri; Sri Mulyaningsih; Sapto Yuliani; Nur Mahdi
Journal of Pharmascience Vol. 12 No. 1 (2025): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v12i1.21350

Abstract

Mangga kasturi (Mangifera casturi Kosterm.) merupakan tanaman endemik Kalimantan Selatan yang dikenal dengan buahnya yang memiliki rasa manis dan aroma khas. Tanaman ini tergolong langka dan telah mengalami penurunan populasi di habitat aslinya akibat deforestasi dan kurangnya upaya budidaya. Daun mangga kasturi mengandung senyawa bioaktif seperti flavonoid dan fenolik yang berpotensi sebagai antioksidan alami untuk kajian awal pengembangan obat. Penelitian ini bertujuan untuk mengidentifikasi kandungan flavonoid dan fenolik dalam ekstrak etanol daun mangga kasturi (EEDMK) melalui uji kualitatif dan kuantitatif. Uji kualitatif dilakukan menggunakan uji tabung dan kromatografi lapis tipis (KLT) dengan fase diam silika gel GF254. Fase gerak berupa campuran heksana, etil asetat, dan asam format (6:4:0,2) digunakan untuk flavonoid, sementara campuran CHCl3, metanol, dan aquadest (7:3,5:1) digunakan untuk fenolik. Penetapan kadar flavonoid dilakukan dengan metode spektrofotometri AlCl₃ menggunakan standar kuersetin, sedangkan senyawa fenolik dianalisis menggunakan metode Folin-Ciocalteau dengan standar asam galat. Hasil uji kualitatif menunjukkan adanya senyawa flavonoid dengan nilai Rf 0,43 dan 0,63 serta fenolik dengan Rf 0,1. Uji kuantitatif menunjukkan total kandungan flavonoid dalam ekstrak adalah 8,3625±0,05 mg QE/g, sementara kandungan fenolik total mencapai 219,0196±1,80 mg GAE/g. Hasil ini menunjukkan potensi EEDMK sebagai sumber senyawa bioaktif yang dapat dikembangkan lebih lanjut untuk sediaan farmasi.
Antibacterial Activity of Endophytic Fungi Isolated from Betel Leaf (Piper betle) Against Pseudomonas aeruginosa and Staphylococcus aureus Kintoko Kintoko; Khrisna Agung Cendekiawan; Sapto Yuliani; Siti Nur Hasana; Firdha Aprilia Wardhani
Jurnal Ilmiah Farmako Bahari Vol 17 No 2 (2026): Jurnal Ilmiah Farmako Bahari
Publisher : Faculty of Mathematic and Natural Science, Garut University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jifb.v17i2.43506

Abstract

Betel leaf (Piper betle) contains antibacterial compounds such as flavonoids, saponins, and alkaloids. Endophytic fungi within plant tissues can produce similar bioactive metabolites. This study aimed to isolate, identify, and evaluate the antibacterial activity of endophytic fungi from young betel leaves against Pseudomonas aeruginosa and Staphylococcus aureus. This was experimental research. Endophytic fungi were isolated from young betel leaves on Potato Dextrose Agar (PDA) and identified macroscopically and microscopically. Antibacterial activity was tested using the paper disk agar diffusion method. Three endophytic fungal isolates were obtained: Isolate 1 (Trichoderma sp.), Isolate 2 (Rhizoctonia sp.), and Isolate 3 (Fusarium sp.). Against P. aeruginosa, Isolate 2 produced an average inhibition zone of 7.33 mm, and Isolate 3 produced 8.00 mm. Against S. aureus, Isolate 1 produced 7.33 mm and Isolate 3 produced 7.33 mm. No inhibition was observed for the negative control (DMSO). Endophytic fungi from betel leaves, particularly isolates resembling Rhizoctonia sp., Fusarium sp., and Trichoderma sp., show weak to moderate antibacterial activity against P. aeruginosa and S. aureus under the tested conditions. Further studies using molecular identification and purified extracts are recommended.
Self-Nano Emulsifying Drug Delivery System (SNEDDS) Kurkumin sebagai Kandidat Agen Terapi pada Model Hewan Demensia Paneri, Jerry Dwi; Wahyuningsih, Iis; Yuliani, Sapto
Indonesian Journal of Pharmaceutical Science and Technology Vol 13, No 2 (2026)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kurkumin, yang berasal dari Curcuma longa, menunjukkan potensi neuroprotektif, namun aplikasi klinisnya terbatas oleh bioavailabilitas yang rendah. Sistem penghantaran obat seperti self-nanoemulsifying drug delivery system (SNEDDS) dikembangkan untuk meningkatkan bioavailabilitas kurkumin. Penelitian ini bertujuan untuk menentukan proporsi optimal minyak kelapa murni (virgin coconut oil/VCO), Tween 80, dan PEG 400 dalam formulasi SNEDDS kurkumin. Penelitian ini meliputi optimasi formulasi menggunakan desain simplex lattice, karakterisasi fisikokimia, evaluasi in vivo, serta analisis statistik menggunakan SPSS. Formulasi SNEDDS yang optimal terdiri atas VCO (11%), Tween 80 (65,66%), dan PEG 400 (23,34%), dengan dosis kurkumin sebesar 35 mg/5 mL. Formulasi ini menunjukkan waktu emulsifikasi sebesar 32 ± 0,83 detik, transmitansi 99,39% ± 0,22%, ukuran droplet 13,18 ± 0,03 nm, indeks polidispersitas 0,131 ± 0,004, serta potensial zeta −13,0 ± 1,74 mV. Studi in vivo menunjukkan bahwa pemberian SNEDDS kurkumin pada dosis 7 mL/kg dan 12 mL/kg berat badan secara signifikan meningkatkan memori spasial pada tikus model demensia. Peningkatan kognitif tersebut tidak menunjukkan perbedaan yang signifikan dibandingkan dengan kelompok yang diberi sitikolin. Temuan ini menunjukkan bahwa SNEDDS yang diformulasikan dengan VCO, Tween 80, dan PEG 400 mampu meningkatkan penghantaran kurkumin dan memperbaiki memori spasial, sehingga mendukung potensinya sebagai terapi neuroprotektif.
Co-Authors Adhi Gunawan Adi Permadi, Adi Ainiyah, Nadia Putri Aisha Raihana Anasyua, Fairuz Khairunnisa Anggoro Wicaksono Anggraeni, Linda Suci Any Guntarti Arif Budi Setianto Arsyannur Pratiwi Aspamufita, Nuroh Ayu Shabrina Ayu Wulandari Azali, Aulia Syafadilla Bachri, Mochammad Saiful Bahagia, Tasya Botutihe, Lisa Agustina Citra Ariani Edityaningrum Daffara Kinanthi Lustikaiswi Daipadli Daipadli Danang Prasetyaning Amukti Dandy Annas Muttaqien Davit Nugraha Desi Ambarwati Desi Iswahyuni Didik Yuni Prasetya Dwi Utami Elmi Rahmadani Enti Rikomah, Setya Fairuz Zahroh Fitri Rahmawanti Galuh Rista Putri Gina Lestari Gina Lestari, Gina Gunawan, Adhi Handayani, Tiara Rizki Handayani, Tiara Rizki Hartati, Meity Hasana, Siti Nur Ibdal Satar Iis Wahyuningsih Intan Dwi Rahmita Khozanatul Ulwy Khrisna Agung Cendekiawan Kintoko Kintoko Kintoko Kintoko, Kintoko Laela Hayu Nurani Lasanudin, Rindi Ibrahim Leni Setiani Lily Noor Falah Lily Noor Falah Ma'ruf, Muhammad Magfirah Septiani Yusuf Maghfirah, Rahmayani Maysara, Risha Moch Saiful Bachri Moch Saiful Bachri Moch. Saiful Bachri Mochammad Saiful Bachri Muhammad Alfian, Muhammad Muhammad Ma'ruf Muhammad Marwan Ramadhan Muhammad Nuh Muhammad Yusron Maulana El-Yunusi Mustofa Ahda Nadia Putri Ainiyah Nadia Selvia Najini, Robby Ningrum, Lailatul Fitria Nining Sugihartini Nur Mahdi Nuri Ari Efiana Nurkhasanah Nurkhasanah Mahfudh Nurkhasanah Nurkhasanah Nuroh Aspamufita Paneri, Jerry Dwi Pelita, Putri Dina Permadi, Adi Putri Dina Pelita Rabiatul Adawiyah Rahmah Annura Rahmah Niar Ulfah Rahmawati, Dhea Rahmayani Maghfirah Ramadhan, Muhammad Marwan Ratih Saridewi Reka Rukmiati Rifka Salsabila Rikadyanti Rikadyanti Rindi Ibrahim Lasanudin Risha Maysara Risha Maysara Risma Yuliawanti Robby Najini Safira, Rizka Safwan Safwan Saraswati, Yasti Rizkandar Sari, Eka Anisa Satar, Ibdal Siti Nur Hasana Sofina Rahmadita Sri Mulyaningsih Sri Mulyaningsih Suci Kurniati Sugiyanto Suwidjiyo Pramono Tasya Bahagia Tiara Rizki Handayani Ulfa Ramdhani M Uswatun Chasanah Vivi Sofia Vivi Sophia Wahyu Widyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Wardhani, Firdha Aprillia Yuliawanti, Risma