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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Akuakultur Rawa Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Makara Journal of Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
MUNTI YUHANA
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan - IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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Journal : Microbiology Indonesia

 1 
Free-Living Ice-Nucleating Active Bacteria from High Mountain Lake Habitats MUNTI YUHANA; KURT HANSELMANN
Microbiology Indonesia Vol. 2 No. 3 (2008): December 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.444 KB) | DOI: 10.5454/mi.2.3.7

Abstract

We collected the culturable heterotrophic bacteria from oligotrophic high mountain lake habitats and tested their capability to induce ice formation. Direct plating was carried out using low-nutrient medium at a temperature of between 3 and 4°C. As many as 84 isolates were recovered from glacial ice and natural biofilm growing on granite rocks surface. Six out of 84 isolates were capable of expressing the ice-nucleation phenotype. After autoclaving the cell suspension at 121°C for 15 min, isolate J78 was still able to retain the ability for ice formation. Heat-stable ice nuclei produced by ice-nucleating active bacteria have potential applications in biotechnology. Characterization of INA bacteria was performed employing live-dead Gram staining and molecular methods. Universal primers for Bacteria (S-D-Bact-0008-b-S-20 and S-D-Bact-1524-a-A-18) were used for PCR to amplify almost the full length of the 16S rRNA genes of selected INA isolates. Restriction fragment length polymorphism analysis resulted in 2 unique patterns, as represented by J43 and J83, respectively. Based on DNA sequencing of 16S rRNA gene, isolate J43 (GeneBank accession no. AJ864852) was closely related to Pseudomonas mephitica (99.2% sequence similarity) and Janthinobacterium lividum (99% similarity), whereas isolate J83 (GeneBank accession no. AJ864859) showed 100% sequence identity to Pseudomonas fluorescens.
Effect of Micro-encapsulated Synbiotic at Different Frequencies for Luminous Vibriosis Control in White Shrimp (Litopenaeus vannamei) WAODE MUNAENI; MUNTI YUHANA; WIDANARNI WIDANARNI
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.24 KB) | DOI: 10.5454/mi.8.2.5

Abstract

The aim of this study was to evaluate the effect of micro-encapsulated synbiotic application at different frequencies for luminous disease control in white shrimp (Litopenaeus vannamei). The luminous disease is caused by Vibrio harveyi. In this experiment, a synbiotic which was a combination of the probiotic Bacillus sp. NP5 RfR and the oligosaccharides from sweet potato (Ipomea batatas L.) jago variety was apllied. The synbiotic was encapsulated by spray drying method. The in vivo experiment was conducted by supplementing the shrimp’s diet with the micro-encapsulated synbiotic for 40 days. Treatments included the administration micro-encapsulated synbiotic in different frequencies i.e. once a week (A), twice a week (B), daily (C), and without micro-encapsulated synbiotic (control treatment). The control treatment consisted of positive (K+) and negative (K-) controls. After 30 days period, all of the shrimp were challenged by intramuscular injection of pathogenic V. harveyi RfR at a concentration of 106 CFU ml-1 except the negative control. The treatment C resulted in significantly higher survival rate (SR), specific growth rate (SGR), and immune responses than those of the controls, whereas the feed conversion ratio (FCR) was lower than the controls. In addition, the population of Bacillus sp. NP5 RfR and total bacterial count (TBC) in the intestines increased, whereas the population of V. harveyi RfR and the total vibrio count (TVC) were lower compared to the controls.
In Vitro Phytochemical and Inhibitory Potential Test of Bawang Hutan Bulb Extract (Eleutherine palmifolia) on Vibrio harveyi WAODE MUNAENI; ARMAN PARIAKAN; LAODE BAYTUL ABIDIN; MUNTI YUHANA
Microbiology Indonesia Vol. 11 No. 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.507 KB) | DOI: 10.5454/mi.11.3.1

Abstract

The objectives of this study were to analyze phytochemical content of bawang hutan bulbs extract (Eleutherine palmifolia) and to test the inhibitory potential of bawang hutan bulbs extract on the growth of Vibrio harveyi bacteria at different doses. This study was conducted in March-May 2017 in Testing Laboratory of Fisheries and Marine Science Faculty of Halu Oleo University and Laboratory of Fish Health of Aquaculture Department of Fisheries and Marine Science Faculty and Laboratory of Biopharmaca of Bogor Agricultural University. Test parameter included: (1) Phytochemical test through the method of color visualization, (2) Inhibitory potential test using two methods namely agar diffusion and co-culture. Treatment of dose consisted of positive control/K+ (Chloramphenicol 30 mg/ml), negative control/K- (Sterile Aquadest) and treatment of extract included A (20 mg/ml), B (40 mg/ml), C (60 mg/ml), D (80 mg/ml). Qualitatively, result of phytochemical test showed that bawang hutan bulbs extract contained flavonoid, tannin, saponin, quinone, steroid and triterpenoid compounds. Result of inhibitory potential test indicated that treatment D obtained the highest inhibitory potential, while the minimum inhibitory potential was found in treatment A. The best co-culture test result was also found in treatment D, in which 24 hours after co-culture was performed, no V. harveyi colonies (total bacteria of 0 CFU/mL) were found. Bawang hutan bulbs extract in this study was able to inhibit the growth of V. harveyi.
Co-Authors , Alimuddin , Rahman, , Achmad Farouq Ade Dwi Sasanti Adna Sumadikarta ADNI OKTAVIANA Afif, Usamah Afiff , Usamah Agus Alim Hakim Aldy Mulyadin Aliati Iswantari Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela M Lusiastuti ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Zubaidah Anja Meryandini Annisa Astri Anggraeni Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Ayi Santika Ayi Santika Bako, Surandha Cahyawati, Nadia Citarasu, Thavasimuthu Claritha Madonsa Claritha Madonsa Darna Andrian Ramadhan DEDI JUSADI Destianingrum, Nurin Dalilah Ayu Dinamella Wahjuningrum Eddy Supriyono Efianda, Teuku Reza Emei Widiyastuti Enang Harris Enang Harris Enang Harris Endang Susianingsih Endang Susianingsih Endang Susianingsih Engelhaupt, Martin Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Faoziyatunnisa, Nurul Fauzi, Arini Resti Filibertus Tantio Firdausi, Amalia Putri Fitriana Nazar Gustilatov, Muhamad Hamida Pattah Hamsah Hamsah Hary Krettiawan Hasan Nasrullah Hasan Nasrullah Hermawaty Abubakar Hessy Novita Hessy Novita HORATH, THOMAS Iis Sumartini Iman Rusmana Ince Ayu Khairana Kadriah Ince Ayu Khairana Kadriah Ince Ayu Khairani Kadriah Inem Ode Inka Destiana Sapitri Irzal Effendi Istiqomah, Amalia Jr., Muhammad Zairin Julie Ekasari Ketut Sugama Ketut Sugama Khasanah, Noviati Rohmatul Kukuh Nirmala KURT HANSELMANN Kustiariyah Tarman La Ode Baytul Abidin La Ode Muhammad Arsal Laely Nuzullia Lili Sholichah Lilik Setiyaningsih Linuwih Aluh Prastiti M. Zairin Junior Majariana Krisanti Marini Wijayanti Mia Setiawati Mira Mawardi Muhammad Arif Mulya Muhammad Zairin Jr. Muharijadi Atmomarsono Nasrullah, Hasan Neltje Nobertine Palinggi Nugroho, Riyanto Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah Nuzullia, Laely Ode, Inem Pariakan, Arman Putra, The Best Akbar Esa Putri Shandra Ramhirez Putri, Shofii Amaliah Ramhirez, Putri Shandra Ranta Sumadi Rasidi Rasidi Rasidi, Rasidi Ricko Reynalta Rini, Adityawati Fajar Ronald Kriston Sauttua Nainggolan Rr. Bellya Anasti Maharani S.H. Dwinanti Salamah Salamah Sefti Heza Dwinanti Soko Nuswantoro Sri Hariati Sri Nuryati Sudrajat, R Herman Sugeng Santoso Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Syamsiar, Syamsiar Tambun, Andreas Taufiq Abdullah, Taufiq Tri Heru Prihadi Tsani Untsa, Agista Usman Usman Usman Usman UTUT WIDYASTUTI WAODE MUNAENI WAWAN ABDULLAH SETIAWAN Wicaksono, Baref Agung WIDANARNI WIDANARNI Woro Nur Endang Sariati Yanti Inneke Nababan Yunarty Yunarty Yusli Wardiatno Zakki Zainun Zulfani, Anisa