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All Journal Biospecies Biotika: Jurnal Ilmiah Biologi Biosfer: Jurnal Tadris Biologi Proceeding International Conference on Global Resource Conservation Prosiding Seminar Biologi Jurnal Sumberdaya Hayati Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Industria: Jurnal Teknologi dan Manajemen Agroindustri Jurnal Belantara AL-HAYAT: Journal of Biology and Applied Biology Floribunda Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Riset Biologi dan Aplikasinya Jurnal Biosense Bioscientist : Jurnal Ilmiah Biologi Jurnal Sains dan Teknologi BIO-SAINS | Jurnal Ilmiah Biologi
Muhammad Rifqi Hariri
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Plant Species In The Honggoderpo Cemetery, Wonosobo Regency, Central Java Irsyam, Arifin Surya Dwipa; Ristiawan, Hani; Hariri, Muhammad Rifqi; Peniwidiyanti, Peniwidiyanti; Irwanto, Rina Ratnasih
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.214

Abstract

Honggoderpo Cemetery is one of the urban green open spaces (RTH) which is located adjacent to the center of Wonosobo Regency, Central Java. This cemetery is a sacred area, so disturbances to the environment are rare, including plants. Therefore, plant diversity is still maintained...
Short Notes on the Leaf Architecture and Morphometry of Syzygium spp. Leaves from the Living Collections of Bogor Botanic Gardens Zahro, Shofiyyatuz; Wahidah, Baiq Farhatul; Hariri, Muhammad Rifqi
Biosfer: Jurnal Tadris Biologi Vol 14 No 2 (2023): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v14i2.18128

Abstract

Studies of leaf architecture and morphometry in Syzygium spp. are investigated. Seven species of Syzygium are taken for the study with additional Psidium guineense Sw. used as comparison. The seven species include Syzygium urceolatum subsp. palembanicum (Miq.) P.S.Ashton, Syzygium tinombalum Sunarti, Syzygium sexangulatum (Miq.) Amshoff, Syzygium hirtum (Korth.) Merr. & L.M. Perry, Syzygium boerlagei (Merr.) Govaerts, Syzygium racemosum (Blume) DC., and Syzygium claviflorum (Roxb.) Wall ex Steud. A comprehensive description of leaf architecture for the studied taxa was provided including leaf type, laminar shape, leaf attachment, laminar length, laminar width, petiole, leaf margin, apex shape, base shape, leaf color, and laminar symmetry. The pattern showed primary vein framework, major secondary spacing, major secondary angle, major secondary vein framework, and tertiary veins. Taxonomically significant leaf features of examined taxa showed great diversity in leaf attachment opposite, primary vein framework pinnate, and major secondary angle smoothly decreasing towards base.ABSTRAK: Kajian arsitektur daun dan morfometri Syzygium spp. diamati dan dilaporkan. Tujuh spesies Syzygium digunakan dan satu spesies Psidium guineense Sw. sebagai pembanding. Jenis Syzygium yang diamati terdiri dari Syzygium urceolatum subsp. palembanicum (Miq.) P.S.Ashton, Syzygium tinombalum Sunarti, Syzygium sexangulatum (Miq.) Amshoff, Syzygium hirtum (Korth.) Merr. & L.M. Perry, Syzygium boerlagei (Merr. Govaerts), Syzygium racemosum (Bl) DC., dan Syzygium claviflorum (Roxb.) Wall ex Steud. Deskripsi arsitektur daun yang diamati mencakup ciri tipe daun, bentuk helaian, letak daun, panjang helaian daun, lebar helaian daun, tangkai daun, tepi daun, ujung daun, pangkal daun, warna permukaan daun, dan simetri helaian daun. Pola venasi menunjukkan primary vein framework, major secondary spacing, major secondary angle, major secondary vein framework, dan tertiary veins. Ciri dengan nilai signifikan ditemukan pada letak daun berhadapan, primary vein framework menyirip, dan sudut sekunder utama yang secara halus berkurang menuju dasar.
Decay Tree Detection in Bogor Botanic Gardens Collection Using Sonic Tomograph Technology Zulkarnaen, Rizmoon Nurul; Rinandio, Dipta Sumeru; Hariri, Muhammad Rifqi; Hutabarat, Prima Wahyu Kusuma; Martiansyah, Irfan; Wardani, Fitri Fatma; Mujahidin, Mujahidin; Primananda, Enggal; Rachmadiyanto, Arief Noor; Mulyani, Melza; Husaini, Iin P.A; Setyanti, Dwi; Indresputra, Faozan; Rahmaningtiyas, Lutfi; Pratiwi, widya Ayu; Damayanti, Frisca; Endewip, Lidya N.; Helmanto, Hendra; Suhatman, Agus; Safarinanugraha, Dina; Purnomo, Danang Wahyu
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.7725

Abstract

Bogor Botanic Gardens is an ex-situ plant conservation area in Indonesia. Since BBG is 103 years old, many collections are 100 years old or older. These antique collections may sustain damage, such as broken or collapsing, endangering visitors and employees. As a result, monitoring tree health at BBG is a critical task. According to the tree health monitoring data, 73 of 244 trees were further checked using the PiCUS Sonic Tomograph. Trees from the Fabaceae (31%) and Myrtaceae (10%) families were the most frequently checked. Walnuts trees from the Burseraceae family had the most specimens (47,94%). The PST effectively provides an immediate picture of the stem condition by calculating solid and decaying wood percentage values.
Anatomical Characteristics of Genus Cassine (Celastraceae) Leaves Muliyah, Evi; Yusniati, Euis; Hariri, Muhammad Rifqi; Zulkarnaen, Rizmoon Nurul
Biosfer: Jurnal Tadris Biologi Vol 16 No 1 (2025): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v16i1.25780

Abstract

Cassine is a genus of plants belonging to the family Celastraceae. Species of Cassine are among those preserved through ex-situ conservation efforts at the Bogor Botanical Gardens. This study aims to provide a comprehensive overview of the anatomical characteristics of leaf stomata in four distinct Cassine species. Stomatal and epidermal observations were conducted using the replica method. The findings reveal that all Cassine species exhibit anisocytic stomata, characterized by a central guard cell surrounded by three neighboring cells of unequal size. Furthermore, stomata were confined to the abaxial (lower) surface of the leaf, with none present on the adaxial (upper) side. This distribution indicates that gas exchange in Cassine leaves primarily occurs on the lower surface, reflecting a significant physiological adaptation. These observations offer valuable insights into the structural and functional traits of Cassine species conserved at the Bogor Botanical Gardens. Karakteristik Anatomi Daun Genus Cassine (Celastraceae) ABSTRAK: Cassine adalah genus tanaman yang termasuk dalam keluarga Celastraceae. Tanaman Cassine merupakan salah satu spesies yang dilestarikan dalam konservasi ex-situ di Kebun Raya Bogor. Penelitian ini berfokus untuk memberikan gambaran yang komprehensif mengenai karakteristik anatomi stomata daun pada empat spesies Cassine yang berbeda. Pengamatan stomata dan epidermis diperoleh dengan menggunakan metode replika. Temuan penelitian mengungkapkan bahwa semua spesies Cassine menunjukkan stomata anisositik, yang ditunjukkan oleh sel penjaga pusat yang dikelilingi oleh tiga sel yang berdekatan dengan berbagai ukuran. Selain itu, stomata terbatas pada permukaan abaksial (bawah) daun, tanpa ada yang ada di sisi adaksial (atas). Hal ini menunjukkan bahwa pertukaran gas pada daun Cassine terutama terjadi di bagian bawah, yang mencerminkan adaptasi fisiologis yang penting. Pengamatan ini memberikan wawasan yang berharga tentang sifat struktural dan fungsional dari spesies Cassine yang disimpan di Kebun Raya Bogor.
OPTIMASI SUHU ANNEALING MARKA MOLEKULER SIMPLE SEQUENCE REPEAT (SSR) UNTUK STUDI KERAGAMAN GENETIK Aglaonema pictum (Roxb.) Kunth Maharani, Fira Shabrina Bintang; Hariri, Muhammad Rifqi; Turhadi, Turhadi
JURNAL BIOSENSE Vol 8 No 4 (2025): Edisi Oktober 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i4.5695

Abstract

Aglaonema pictum (Roxb.) Kunth merupakan tanaman hias populer di dunia karena memiliki variasi corak warna daun. Studi tentang keragaman genetik A. pictum masih jarang dilakukan, sehingga upaya ini perlu dilakukan. Salah satu penanda molekuler yang dapat digunakan untuk mengetahui keragaman genetik yaitu Simple Sequence Repeats (SSR). Tujuan penelitian ini adalah mengidentifikasi kondisi optimum pada primer SSR untuk studi keragaman genetik A. pictum. Metode yang digunakan adalah pengumpulan sampel, ekstraksi DNA menggunakan cetyltrimethylammonium bromide (CTAB), amplifikasi DNA menggunakan primer SSR (OSR13, RM10, RM6470, OSR14, MRG2172_RT2172, MRG2153_RGP2153, RM249, RM100, MRG2881_RT2881, dan RM7240) dengan menggunakan Polymerase Chain Reaction (PCR) pada kisaran suhu 52-54℃, 55-59℃, 47.2-50.9℃, dan 47.6-50.4℃, elektroforesis dan dokumentasi gel agarosa, serta analisis data. Hasil penelitian menunjukkan bahwa dua dari sembilan primer SSR (RM249 dan RM7240) dapat diamplifikasi dengan baik pada suhu 49,3 ˚C dan 52,6 ˚C. Kedua suhu tersebut mengindikasikan suhu optimum yang dapat mengamplifikasi DNA sampel A. pictum. Sementara itu, primer OSR13, RM10, RM6470, OSR14, MRG2172_RT2172, MRG2153_RGP2153, RM100, MRG2881_RT2881 tidak berhasil teramplifikasi dengan baik dan spesifik pada rentang suhu annealing yang digunakan dalam penelitian ini.
Pemetaan Spesies Asing Invasif Hydrocotyle leucocephala Cham. & Schltdl. di Kebun Raya Bogor Solihah, Fifi Mar’atun; Hariri, Muhammad Rifqi; Turhadi, Turhadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10841

Abstract

The presence of undocumented alien flora threatens Indonesia’s flora richness and diversity due to unpredictable invasion risks. Hydrocotyle leucocephala is an alien flora that has been reported as invasive in Sri Lanka and Nigeria and has distributed to Indonesia. Therefore, mapping the distribution and describe the morphological of Hydrocotyle leucocephala is necessary to detect the initial introduction or further spread of this species. The data collection was carried out by exploring the Bogor Botanical Gardens. Environmental factors are measured and documented where Hydrocotyle leucocephala occurs. For observation, Hydrocotyle leucocephala was taken and their morphology described. As a results, Hydrocotyle leucocephala was spread over vak V.L, V.G, V.M, IX.D, VIII.B and parking lot near the conservation building in the Bogor Botanical Gardens. It is also known that Hydrocotyle leucocephala is a creeping herbaceous plant with glabrous stems, kidney-shaped leaves, white flowers without petals and green fruit with persistent styles.
DNA EXTRACTION FROM LOCAL SOUVENIR OF SUKU ANAK DALAM JAMBI WITHOUT LIQUID NITROGEN USING CTAB METHOD Wulandhari; Damayanti, Inggar; Bintoro, Afif; Hariri, Muhammad Rifqi
Jurnal Belantara Vol 7 No 1 (2024)
Publisher : Forestry Study Program University Of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbl.v7i1.1036

Abstract

One of the local souvenirs from Jambi is accessories made from the seed bark of the sebalik sumpah tree. The sebalik sumpah tree is currently quite rare. Through DNA isolation, sebalik sumpah is one of the efforts of genetic conservation and as a first step in cultivating sebalik sumpah trees. DNA isolation is the initial stage in molecular analysis. The purpose of DNA isolation is to obtain genomic DNA from a sample. Plant DNA isolation generally uses fresh plant samples, with young and soft plant parts. This is recommended to obtain good quality genomic DNA. However, with the limitations of fresh samples, a method is needed that is able to extract DNA from plants that have changed shape and have undergone processing. Each plant requires a different method to obtain DNA. The CTAB (Cethyl Trimethyl Ammonium Bromide) buffer method is a method commonly used in plants because it is able to extract DNA with high polysaccharide and phenol content, so many have modified the method. This study aims to determine the success of the CTAB method without nitrogen in extracting processed materials and the length of incubation time at the precipitation stage. Extraction was carried out by liquid nitrogenless CTAB method with the addition of PVP (Polyvinylpolypyrrolidone). The results show that the CTAB method without liquid nitrogen is able to extract plant samples that have undergone processing and the length of incubation time at the precipitation stage affects the DNA produced.
Diversity, Vegetation Structure, Estimated Biomass and Carbon Stock in The Site of Limboto Lake of Gorontalo Regency Rahim, Sukirman; Baderan, Dewi Wahyuni K; Arfa, Bella Saskia; Angio, Melisnawati H; Hariri, Muhammad Rifqi; Putri, Dwinda Mariska
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23010

Abstract

The development of Limboto Lake  Site as the pilot area of Gorontalo Geopark is one step  in rescuing the lake. This research aims to analyzed  the vegetation structure, estimate biomass, and assess the carbon stock value of the vegetatio at the Limboto Lake site. This study employed a purposive sampling method. Important Value Index, a measure used to evaluate plant vegetation structure, was calculated based on Realtive Density, Relative Dominance, and Relative Frequency values. Biomass estimation and carbon stock assessment were conducted using allometric equations. The study’s finding showed that the vegetation surrounding Soekarno's landing area exhibited the highest IVI (sawlogs) scores for Swietenia mahagoni tree at 82.86%, Leucaena leucocephala saplings at 79.56%, and Manihot esculenta seedlings at 46.33%. Leucaena leucocephala consistently achieved the highest IVI score within the Limboto Lake area with a score as high as 76.90%, followed by Leucaena leucocephala on the sapling level with a score as high as 70.03%, and Nauclea orientalis on the seedling level with a score as high as 53.06%. Swietenia mahagoni, Samanea saman, and Leucaena leucocephala are three species with stems that predominate at the sawlog level and sapling, and these stems have high carbon absorption values.
SUHU ANNEALING PRIMER INTER SIMPLE SEQUENCE REPEAT (ISSR) PADA DNA Aglaonema pictum (Roxb.) Kunth Baharuddin, Muh. Fikry; Hariri, Muhammad Rifqi; Turhadi, Turhadi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 11 No. 1 (2026): Bioma : Januari - Juni 2026
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v11i1.45426

Abstract

Aglaonema pictum is one of the most popular ornamental plants in the community. However, studies on the genetic diversity of A. pictum are still limited. Based on this, it is necessary to study the genetic diversity of A. pictum, one of which uses DNA-based markers such as Inter Simple Sequence Repeats (ISSR). In order to study of genetic diversity of A. pictum effectively and efficiently, one of the efforts that can be done is optimizing the annealing temperature for ISSR primers to be used. The purpose of this study was to optimize the effective Inter Simple Sequence Repeats (ISSR) primers on DNA of A. pictum. The methods used include DNA extraction using cetyltrimethylammonium bromide (CTAB), DNA amplification using primers ISSR1, ISSR2, ISSR3, ISSR4, ISSR5, ISSR6, ISSR7, ISSR8, ISSR9, ISSR10, ISSR11, and ISSR12) using Polymerase Chain Reaction (PCR) at temperature ranges of 52-54 ˚C. The results showed that ten out of 12 ISSR primers could amplify the DNA of A. pictum using optimal annealing temperatures. ISSR primers which identified optimum annealing temperature were ISSR1, ISSR3, ISSR5, ISSR6, ISSR7, ISSR8, ISSR9, ISSR10, ISSR11, and ISSR12. Primer of ISSR1 and ISSR11 showed optimum annealing temperature at 52,6°C. Primer of ISSR3, ISSR8, and ISSR9 showed optimum annealing temperature at 53,6°C, while primer of ISSR6, ISSR7, and ISSR10 showed optimum annealing temperature at 54°C. in other hands, primer of ISSR2 nad ISSR4 did not yet identified their optimum annealing in all tested temperature range. Kata kunci : Aglaonema pictum, Annealing Temperature, Genetic Diversity, Inter Simple Sequence Repeats
DISTRIBUSI KONYAL (Passiflora suberosa L.) DI KEBUN RAYA BOGOR, JAWA BARAT Althoriq, Luthfi; Hariri, Muhammad Rifqi; Turhadi, Turhadi
Biospecies Vol. 19 No. 1 (2026): Januari 2026
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v19i1.46650

Abstract

Konyal (Passiflora suberosa L.) is a wild and invasive plant belonging to the Passifloraceae. The existence of P. suberosa as an invasive species can threaten the existence of local plants. As a scientific conservation area, the Kebun Raya Bogor (KRB) is a habitat for Konyal. One effort that can be made to minimize the threat of invasive species is through mapping. The aim of this research was to map the distribution of konyal (P. suberosa) in the scientific conservation area of Kebun Raya Bogor and measure environmental factors in the habitat of konyal (P. suberosa). The research was carried out using an exploratory method throughout the Kebun Raya Bogor area in January 2024. Documentation of komyal (P. suberosa) and measurements of environmental factors in its growing habitat were carried out during exploring activities. In addition, distribution coordinate data was recorded and used for mapping using QGIS v3.36.2 program. The research results showed that the konyal  (P. suberosa) in the KRB was spread over six areas, including II A, II C, II D, II F, III I, and  XIII A. Environmental factors at the location where P. suberosa was found in the Kebur Raya Bogor area, such as air humidity (70 – 77%), temperature (26 – 33 ˚C), and light intensity (2250 – 16200 lux). Distribution of konyal (P. suberosa) in KRB area showed high density in vak II (II A, II C, II D, and II F). The location vak II has vegetation with sparse canopy and low shade, thereby increasing the sunlight intensity compared to other areas.
Co-Authors A Husaini, Iin Pertiwi Achmad Fariz Ade Ayu Oksari Afif Bintoro Afri Irawan Almaidah, Elsa Nadia Althoriq, Luthfi Amy Tenzer Angio, Melisnawati H Aprilianingsih, Risqi Arfa, Bella Saskia Arie Setyawati Arifin Surya Dwipa Irsyam Arifin Surya Dwipa Irsyam ASIH PERWITA DEWI Asih Perwita Dewi ASIH PERWITA DEWI, ASIH PERWITA Atria, Mega Baharuddin, Muh. Fikry Baidlowi, Muhammad Hisyam Baiq Farhatul Wahidah Balqis Balqis Budianti, Noviana Canita, Anggun Damayanti, Frisca Damayanti, Inggar Danang Wahyu Purnomo Dewi Wahyuni K. Baderan DIAN LATIFAH, DIAN Dian Rosleine, Dian Effendi, Surianto eko purnomo Endewip, Lidya N. Fitri Fatma Wardani Handayani, Windri Helmanto, Hendra Husaini, Iin P.A Husaini, Iin Pertiwi A Hutabarat, Prima Wahyu Kusuma Indresputra, Faozan Irsyam, Arifin Surya Dwipa Irwanto, Rina Ratnasih Maharani, Fira Shabrina Bintang MARTIANSYAH, Irfan Melisnawati H Angio Mountara, Arieh Mujahidin Mujahidin Muliyah, Evi Mulyani, Melza Nugrahaningsih Nugrahaningsih Peniwidiyanti Peniwidiyanti Peniwidiyanti, Peniwidiyanti Pratiwi, Widya Ayu Primananda, Enggal Putri, Dwinda Mariska RACHMADIYANTO, ARIEF NOOR Rahmaningtiyas, Lutfi Ria Windi Lestari Rina Ratnasih Irwanto Rinandio, Dipta Sumeru Ristiawan, Hani Rita Rizky Rahmawati Rizki Wulan Anjani Safarinanugraha, Dina Santoso, Trio Saripudin Saripudin Setiawan, Ashari Bagus Setyanti, Dwi Solihah, Fifi Mar’atun Suhatman, Agus Sukirman Rahim Sulistyawati, Endah Syamsidah Rahmawati, Syamsidah Thobib Hasan Al Yamini Tianara, Alexander Turhadi Turhadi Vertiana, Erlin Via Widjaya, Aulia Hasan Wulandhari Yoyo Suhaya Yoyo Suhaya Yuliyanto, Ponco Yusniati, Euis Zahro, Shofiyyatuz Zakaria Al Anshori zulkarnaen, rizmoon Zulkarnaen, Rizmoon Nurul