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All Journal Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Veterinary Biomedical and Clinical Journal Jurnal Ilmu Peternakan Terapan Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary
Dadik Rahardjo, Dadik
Veterinary Public Health Departement, The Faculty of Veterinary Medicine, Airlangga University Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments Sugihartuti, Rahmi; Pradana, Munawer; Maslachah, Lilik; Chusniati, Sri; Tyaningsih, Wiwiek; Rahardjo, Dadik; Soeharsono, Soeharsono
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.58266

Abstract

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
Limousin cattle production performance and farmer income post-FMD fed with super booster and immunobooster concentrate Maghfiroh, Nurutin Tutur Bifatikhatil M; Al Arif, Mohammad Anam; Lamid, Mirni; Mustofa, Imam; Mufasirin, Mufasirin; Rahardjo, Dadik
Jurnal Ilmu Peternakan Terapan Vol 8 No 2 (2025): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v8i2.5795

Abstract

This study aims to evaluate the administration of super booster, and immunobooster concentrates on production performance and farmers' income following an outbreak of foot and mouth disease (FMD) by proving the improvements in production performance, including feed consumption, weight gain, and revenue of crossbred Limousin cattle farmers. This study used 18 male Limousin crossbred cattle selected based on age (1.5-2 years) and having been infected with FMD virus that is divided into three treatments: T0 (control feed consisting of forage and local concentrate), T1 (control feed + 40 g of a super booster), and T2 (control feed + 600 g of immunobooster concentrate). Data collection and observation were conducted over 42 days. Statistical analysis was performed using analysis of variance (ANOVA) based on the completely randomized design (CRD), followed by Duncan’s multiple range test (DMRT). The results of the variance analysis showed a p-value of <0.05, indicating that the addition of super booster and immunobooster can improve feed consumption, body weight gain, and increase farmers income in crossbred Limousin cattle farming following an outbreak of FMD, so that it can be used as an alternative step in overcoming post-FMD losses and helping farmers maximize profits.
Multi-Drug Resistant (MDR) Detection in Escherichia coli in Canary Birds (Serinus canaria) Imported from Malaysia Istiana, Izzatul; Effendi, Mustofa Helmi; Kurniawan, Muhammad ‘Ahdi; Sari, Fifin Kurnia; Purbowati, Tri Endah; Rahmandari, Dina Agylia; Mufasirin; Setiawan, Boedi; Rahardjo, Dadik
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.123-136

Abstract

The frequency of canary imports continues to increase every year. Antibiotic resistance is a global problem that threatens human and animal health worldwide. Human interaction with birds as pets is a public health concern because it has the potential to increase zoonotic diseases. This study was conducted to identify the antibiotic resistance of E. coli using fecal swab samples of imported canaries from Malaysia. Samples were grown on an EMBA medium for the isolation test, Gram staining test, and IMViC test were performed to continue the identification test -Kirby-Bauer diffusion test - to determine antibiotic sensitivity. Based on morphological culture, Gram staining, and biochemical tests, the sample examination results showed 18% (27/150) were positive for E. coli, 16% (24/150) showed the highest resistance to tetracycline, 15,33% (23/150) amoxicillin, 12,66% (19/150) trimethoprim-sulfamethoxazole, 6% (9/150) ciprofloxacin, and 14,66% (22/150) isolates were confirmed MDR because they were resistant to three to four antibiotics. Further efforts are needed to understand and address the factors that lead to antibiotic resistance in the context of animal and public health. Prudent management of antibiotic use and monitoring of antibiotic resistance needs to be improved to maintain animal health and prevent the risk of transmission of resistant bacteria to humans.
The Implementation of Hazard Analysis and Critical Control Point (HACCP) in The Frozen Chicken Meat Distribution Chain in Sikka Regency Dua Bura, Maria Antonia Yersi; Effendi, Mustofa Helmi; Puspitasari, Yulianna; Rahardjo, Dadik; Tyasningsih, Wiwiek; Rachmawati, Kadek
VITEK : Bidang Kedokteran Hewan Vol. 14 No. 2 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i2.289

Abstract

Food safety has become a global issue. Hazard analysis and critical control points (HACCP) is a food safety assurance system in the world that emphasizes prevention efforts. This research is a qualitative descriptive epidemiological study which aims to examine the application of HACCP in the distribution chain of frozen chicken meat in Sikka Regency on the growth parameters of total bacteria, Salmonella, and antibiotic residues through field observations at supplier companies and retailers, as well as laboratory tests on 30 frozen chicken meat samples, and 30 fresh chicken meat samples as a comparison. Laboratory tests found antibiotic residues in 60% of samples (16.7% in fresh chicken meat), and microbial contamination in 33% of samples without Salmonella contamination (40% in fresh chicken meat without Salmonella contamination). Monitoring of antibiotic residues has not been carried out properly by supplier companies because the number of samples is very small, sales in markets without refrigeration facilities for up to five hours together with fresh chicken or near fresh fish allows contamination of chicken meat. HACCP principles have not been properly applied in the frozen chicken meat distribution chain. Guidance and regular monitoring are needed so that the quality of frozen chicken meat is maintained.
POTENSI TEKNOLOGI INSEMINASI BUATAN PADA PENINGKATAN PRODUKTIVITAS ITIK TURI LAMONGAN Restiadi, Tjuk Imam; Hernawati, Tatik; Rahardjo, Dadik; Widiyatno, Thomas V.
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v8i1.2019.82-88

Abstract

Duck is one of the most poultry that potential can be best developed for its productivity to be used for meat and eggs. The obstacles faced by farmers include productivity of low and not meat and duck eggs. Application of science and technology through the development of reproductive technology of Artificial Insemination (AI) by conducting cross-breeding. The application to cross ducks is very beneficial because in addition to being economical it is also efficient. AI duck is very easy to apply and fast, does not require a complicated and long process as in large livestock. Ducks from artificial insemination are produced in Turi local female ducks (Anas plathirinchos) with plump males (Cairina moschata). The reason for the crossing is because the duck man has a body size that is too large compared to the local duck, so it can produce offspring with rapid growth and large body size. The conclusion is that duck farming with IB in Tawangrejo Turi Lamongan is useful for increasing body weight and productivity.
Kualitas semen sapi pejantan berdasarkan umur, suhu, dan kelembaban di Taman Ternak Pendidikan Universitas Airlangga Brillianti, Febby Fairy; Srianto, Pudji; Sardjito, Trilas; Suprayogi, Tri Wahyu; Triana, Indah Norma; Rahardjo, Dadik
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.81-89

Abstract

This research was conducted in the teaching farm of the Faculty of Veterinary Medicine, Universitas Airlangga. The present study aimed to determine the effect of age, temperature, and humidity on bull semen quality which was processed into frozen semen. Three age groups of bulls (young 3-4 years, middle age 5-6 years, and old 7-8 years) were collected for the temperature and humidity of semen examination. Fresh semen was collected using an artificial vagina, followed by macroscopic and microscopic examination. In addition, post-thawing motility was also evaluated. The results showed that there was no effect of temperature and humidity during semen collection process. Fresh semen quality was affected by age. Moreover, the post-thawing motility of fresh semen of spermatozoa was lower than those of the fresh semen; however, it still qualifies for artificial insemination. It could be concluded that the semen of bulls at the Teaching Farm of Universitas Airlangga has good quality, and is suitable for Artificial Insemination.
Co-Authors Al arif, Mohammad Anam Alpha Fardah Athiyyah Andriani, Ratna Andy Darma Arimbi Aryaloka, Suhita Boedi Setiawan Brillianti, Febby Fairy Bura, Maria Antonia Yersi Dua Cholifah, Siti Chusnul Chusniati, Sri Darmawan, Moch. Rizky de Vries, Garry Cores Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Hani Plumeriastuti Hayuning Nurrodhiya I Gusti Made Reza Gunadi Ranuh IMAM MUSTOFA Indah Norma Triana, Indah Norma Istiana, Izzatul Kadek Rachmawati Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Maghfiroh, Nurutin Tutur Bifatikhatil M Margaretha, Josephira Intan Mirni Lamid Mochamad Lazuardi Mufasirin Munawer Pradana Mustofa Helmi Effendi Nadilla Chrisantya Yusuf Putri Nunuk Dyah Retno Lastuti PA, Deanty Ayu Permatasari, Dian Ayu Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastutiek Pudji Srianto Purbowati, Tri Endah Rahmandari, Dina Agylia Rahmi Sugihartuti, Rahmi Retno Lastuti, Nunuk Dyah Risky Aprillian, Risky Rochmah Kurnijasanti Sabdoningrum, Emy Koestanti Sari, Fifin Kurnia Setiawan Koesdarto Shirakawa, Toshiro Soeharsono Soeharsono Sudarmo, Subijanto Marto Sukmanadi, Mohammad Sunarso, Agus Tatik Hernawati Tjuk Imam Restiadi Tri Suwanti, Lucia Tri Wahyu Suprayogi Trilas Sardjito Tyaningsih, Wiwiek Tyasningsih, Wiwiek Warsito, Sunaryo Hadi Widiyatno, Thomas V. Yulianna Puspitasari Yunus, Muchammad