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All Journal Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus ARSHI Veterinary Letters Vitek : Bidang Kedokteran Hewan Journal of Veterinary and Animal Sciences

I Gusti Made Krisna Erawan

Fakultas Kedokteran Hewan, Universitas Udayana, Denpasar Bali

Author-ID : 520023

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Deteksi Produksi Toksin Stx-1 dan Stx-2 dari Escherichia coli O157:H7 Isolat Lokal Hasil Isolasi Feses dan Daging Sapi I Wayan Suardana; I Gusti Made Krisna Erawan; Bambang Sumiarto; Denny Widaya Lukman
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.501 KB)

Shiga toxin produced by Escherichia coli O157:H7 can cause outbreaks and sporadic cases of serioushuman diseases. The diseases are indicated by hemorrhagic colitis and hemolytic uremic syndrome. Meatand meat products have been identified as vehicles of food borne disease caused by E.coli O157:H7. Themain aim of this research was to identify the correlation between the level of E.coli O157:H7 contaminationand the presence of Shiga toxin (Stx1 and Stx2) by applying method of Vero toxin Escherichia coli-ReversePassive Agglutination Test (VTEC-RPLA). The results showed that 3 of 7 isolates and 1 of 4 isolatesisolated from feces of cattle and beef, respectively produced Stx 1 (VT1). In the detection of Stx 2 (VT2), 4of 7 isolates and 1 of 4 isolates, isolated from the same samples were found to produce this toxin.According to all isolates, in this research showed, 1 isolate was found to produce VT2, 4 isolates to produceboth VT1 and VT2, while 6 isolates showed negative results either to VT1 or VT2.

Heart Rate of Reticulated Pythons (Malayopython reticulatus) from Bali based on Its Body Length (DETAK JANTUNG ULAR SANCA BATIK (Malayopython reticulatus) BERDASARKAN PANJANG TUBUH DI BALI) Steven Dwi Purbantoro; Puveanthan Nagappan Govendan; I Gusti Made Krisna Erawan; Zefanya Christiani; Erika Erika; Yedija Putra Kusuma Wardana Rumbay; Slamet Raharjo
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was aimed to determine the correlation between heart rate and body length of reticulated python (Malayopython reticulatus) from the island of Bali. The study offers data of the M. reticulatus heart rate value to exotic animal veterinary practitioner. Fifteen clinically healthy snakes, consists of 9 females and 6 males were used in this study. Heart rate was measured twice a day during day and night using a fetal Doppler (BF-500 eBestman®, China) and body length using a measurement tape. Heart rate was analyzed with independent t-test to time collection and correlation to body length. Results show that heart rate mean value at day and night of the M. reticulatus from the island of Bali was each 65.68±14.093 bpm and 64.53±13.637 bpm. Heart rate value of wild-caught snakes is higher (67.90±15.290 bpm) than captivebred snakes (60.92±10.039 bpm). Total length (TL), snout-vent length (SVL), and snout-heart length (SHL) mean value of M. reticulatus from the island of Bali was each 168.6±90.67 cm, 149.53±82.77 cm, and 38.7±19.28 cm. Correlation between heart rate and total length has higher R-value (R = 0.802), and followed by snout-heart length (R = 0.800) and snout-vent length (R = 0.792). Statistically, it could be concluded that heart rate during day and night time shows no significant difference, heart rate value based on how it is kept is significantly different, and heart rate was negatively correlated to body length.

Gambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali (CYTOLOGIC FIGURES OF PERITONEAL AND SYNOVIAL FLUIDS IN BALI DUCKS) Iwan Harjono Utama; Sri Kayati Widyastuti; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; Ida Bagus Oka Winaya; Ida Bagus Komang Ardana; Achoiro Wati Rasid; Tyas Pandieka Yoga
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.998 KB)

This study aim was to observe cellular composition and morphology in peritoneal and synovial fluids ofbali ducks. This study used 50 ducks, which were composed of 21 males and 29 females aged 8 to 12 weeks.After being declared clinically healthy, the sample ducks were slaughtered and peritoneal fluid specimenfrom the abdominal cavity and the synovial fluid of joints tibiotarsal were collected. Then, in preparationfor the specimen, each of fluid was smeared and stained with Giemsa. Observations were made under amicroscope with a magnification of 1000 times and observations expressed in the average number of cellsper field of view observation. The results showed (duck population that most of the observed sample): inthe peritoneal fluid there was 36% eosinophils observed and 38% had heterophile cells with 0-3 cells perfield of magnification 1000x view. Similarly, 28% ducks had lymphocytes with 13-16 cells, and as much as28% of ducks had macrophage cells with 5- 8 cells in observation . In the synovial fluid, however, 64%sample ducks had monocytes, while 72% having lymphocytes with 1-18 cells each of view field withmagnification 1000x. In addition, in the synovial fluid, as much as 62% of ducks also observed had 1 to 3cells heterophiles and 60% ducks had eosinophils (polimorphonuclear cells) per view field observation.Macrophage cells that were found in the peritoneal fluid were mainly hyper granulated. It can be concludedthat the amount of mononuclear cells in both types of body fluids is likely more dominant compared to thenumber of polymorphonuclear cells.

Antigen Ekskretori-Sekretori Cacing Jantung (Dirofilaria immitis) Jantan dan Betina yang Berpotensi Sebagai Marka Diagnosis (EXCRETORY-SECRETORY ANTIGENS OF MALE AND FEMALE HEART WORMS (DIROFILARIA IMMITIS) WHICH POTENTIALLY AS A DIAGNOSTIC MARKER) I Gusti Made Krisna Erawan; Ida Tjahajati; Wisnu Nurcahyo; Widya Asmara
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.95 KB)

Heart worm (Dirofilaria immitis) is the causative agent of a serious parasitic disease in dogs.Dirofilariasis is generally diagnosed by microfilariae examination and specific antigen testing. Microfilariaeexamination has low sensitivity due to occult infections. The available antigen test at this time is able todetect circulating antigens secreted by adult female worms only. The aim of the present study was toidentify male (MES) and female (FES) heart worms excretory-secretory antigens which have the potentialas a diagnostic targets. Identification of antigen was done by sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE) and Western Blotting analysis. The results of this study indicated that therewere differences between the MES and the FES profiles. The results showed 12 bands in MES (14–118kDa) and 18 bands in FES (10–205 kDa). Protein with a molecular weight of 59 kDa has the potential asdiagnostic markers of dirofilariasis.

Deteksi Anaplasma sp. pada Anjing di Bali secara Klinis, Serologis, dan Molekuler (THE DETECTIONS OF ANAPLASMA SP. IN DOGS IN BALI WITH CLINICAL, SEROLOGICAL AND MOLECULAR) Anak Agung Sagung Istri Pradnyantari; I Nyoman Suartha; I Gusti Made Krisna Erawan; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aims to determine the infection of Anaplasma sp. in dogs in Bali with clinical, serological and molecular detections. This research method uses Cross Sectional. The samples were collected from June 2017 to July 2018. The total number of samples obtained was 109 dogs from 3.563 samples from seven veterinarian clinics in Bali. Clinical examinations and blood tests are method of clinical detection. Serological detection is using a rapid test kit E. canis & Anaplasma spp. BioNote© production. Molecular detection have been using with the Polymerase Chain Reaction technique. PCR products were sequenced at 1st BASE Laboratories Sdn Bhd, Malaysia. Data were analyzed using the MEGA 4 program. The results showed that Anaplasma sp. are present in dogs in Bali and can be detected by clinically (3,05%), serologically (55,04% base on clinically positive) and molecularly (42,20% base on clinically positive) detection. Thus, detected species Anaplasma sp. In Bali from this study can be identified as A. platys.

Urolithiasis oksalat monohidrat pada kucing lokal Iwan Harjono Utama; Sri Kayati Widyastuti; I Gusti Made Krisna Erawan; Ekklesia Prasetya
ARSHI Veterinary Letters Vol. 2 No. 2 (2018): ARSHI Veterinary Letters - Mei 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Kasus urolithiasis oksalat monohidrat dijumpai pada kucing lokal yang datang ke Rumah sakit Hewan Fakultas Kedokteran Hewan Universitas Udayana dengan anamesis kucing mengalami penurunan nafsu makan. Gejala klinis yang terlihat adanya kifosis ringan. Palpasi di abdomen bagian atas hingga belakang dilakukan dan hewan meronta kesakitan saat palpasi di bagian hypogastrium medial vesica urinaria. Kucing juga menunjukkan ekspresi nyeri saat dilakukan palpasi pada bagian penis saat pemeriksaan uretra dan urinasi. Kucing sedikit-sedikit mengeluarkan urin berdarah dengan konsistensi cair. Pemeriksaan lanjut dilakukan menggunakan ultrasonografi dan urinalisis menggunakan uji cepat dipstik. Hasil pemeriksaan ultrasonografi memperlihatkan adanya bintik bintik putih di vesika urinaria, sedangkan pemeriksaan fisik urin memperlihatkan hematuria dan mikroskopik memperlihatkan adanya kristal oksalat monohidrat. Penanganan yang dilakukan pada kasus ini adalah memberikan antibiotik amoxicillin injeksi dosis 11-22 mg/kg bb untuk mengurangi infeksi pada saluran kemih. Selain itu Nephrolit® yang mengandung Orthosiphon stamineus (kumis kucing) dan beberapa herbal lainnya juga diberikan untuk membantu alkalinisasi dan pelarutan kristal-kristal dalam urin agar mudah dikeluarkan saat urinasi. Hasil penanganan memperlihatkan kucing membaik dalam waktu 10 hari terapi.

Case Report: Severe Ticks Infestation with Ehrlichiosis in Mixed Dog Deva Mutiara Giri Putri; Putu Ayu Sisyawati Putriningsih; I Gusti Made Krisna Erawan
Journal of Veterinary and Animal Sciences Vol 6 No 2 (2023)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2023.v06.i02.p05

Abstract. Canine ehrlichiosis is a disease caused by infection of Ehrlichia sp., which is a tick-borne disease. Transmission of ehrlichiosis in dogs can occur through the bite of Rhipicephalus sanguines as a transmission vector. A Shih Tzu-Terrier mixed dog named Mogli was examined with complaints of decreased appetite, weight loss, fever, and severe pruritus. Physical examination showed the presence of petechies, vulnus on the skin and Rhipicephalus sanguineus tick infestation, especially around the eyes and back. Complete hematological examination and blood smear showed leukocytosis, lymphocytosis, thrombocytopenia, increased granulocytes, hematocrit, and the presence of intracytoplasmic bodies in monocytes. The results of the examination with the test kit showed a positive result, which means had antibodies to Ehrlichia sp. Based on the examinations, the dog was diagnosed with severe tick infestation with accompanied ehrlichiosis. The dog was given doxycycline 5 mg/kg BW twice a day orally for 28 days and fluralaner 250 mg orally, tolfenamic acid 4 mg/kg BW intramuscularly (IM), chlorpheniramine maleat 2 mg once daily orally for 14 days, and vitamin B-complex once a day orally for 28 days. After 14 days of treatments showed changes in the dog's appetite that returned to normal, the dog looked active, the dog's body temperature was normal, the infestation of the Rhipicephalus sanguineus ticks significantly reduced, and the frequency of pruritus was reduced. The results of the hematological examination on the fourteenth day showed that all parameters were within the normal range.

Laporan Kasus: Penanganan Pyometra Tertutup Disertai Kalkuli Vesika Urinaria pada Anjing Pomeranian Berusia Tujuh Tahun Bravanasta Glory Rahmadyasti Utomo; I Gusti Agung Gde Putra Pemayun; I Wayan Wirata; I Gusti Made Krisna Erawan; I Wayan Batan
Jurnal Veteriner Vol 24 No 4 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.4.573

Pyometra adalah kondisi peradangan pada endometrium mengakibatkan akumulasi nanah di dalam rahim. Pyometra dapat terjadi secara kronis (pyometra terbuka) dan secara akut (pyometra tertutup). Pyometra tersebut harus segera mendapatkan penanganan karena sifatnya gawat darurat. Masalah kesehatan pada sistem perkencingan juga banyak ditemukan pada anjing, salah satunya adalah kalkuli vesika urinaria (VU). Kalkuli adalah kondisi terbentuknya urolith pada saluran perkencingan terutama di VU. Tujuan penulisan artikel ini adalah untuk mengungkap kejadian pyometra yang berlangsung secara bersamaan dengan kalkuli VU pada anjing. Anjing ras pomeranian, berusia tujuh tahun, jenis kelamin betina, dengan bobot 2,3 kg, dibawa oleh pemiliknya ke Laboratorium Ilmu Bedah Veteriner FKH Unud dengan keluhan terjadi pembesaran pada bagian perut. Berdasarkan anamnesis dan gejala klinis yaitu pembesaran abdomen serta keluarnya leleran berwarna putih dan berbau dari vagina. Pemeriksaan klinis, dan pemeriksaan penunjang yaitu pemeriksaan hematologi rutin, pemeriksaan biokimia darah, menunjukkan leukositosis dan trombositopenia yang parah. Pemeriksaan radiografi rongga abdomen, dan pemeriksaan ultrasonografi, menunjukkan hasil adanya penebalan dinding dan pembesaran uterus serta gambaran hipoekoik di dalam uterus, serta hasil radiografi memperlihatkan adanya massa radiopaque bulat pada VU, anjing menderita pyometra tertutup disertai dengan adanya kalkuli pada VU. Penanganan yang diberikan berupa tindakan pembedahan ovariohysterectomy untuk mengangkat rahim dan sistotomi untuk mengeluarkan batu dari dalam VU. Premedikasi yang diberikan adalah Atropine Sulfate dengan dosis 0,02 mg/kg BB secara subkutan dan kemudian diinduksi menggunakan kombinasi Xylazine 0,87 mg/kg BB dan Ketamin 4,35 mg/kg BB secara intravena. Selama operasi diberikan tambahan anestesi dengan dosis yang sama dengan dosis awal pemberian. Pada hari kedua setelah operasi kondisi anjing menurun dan akhirnya mati.

Laporan Kasus: Sparganosis dan Ankilostomiosis pada Kucing Lokal Burhan, Haris; Batan, I Wayan; Erawan, I Gusti Made Krisna
Indonesia Medicus Veterinus Vol 12 (5) 2023
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2023.12.5.713

Sparganosis adalah penyakit zoonosis yang ditularkan melalui makanan yang disebabkan oleh larva plerocercoid (spargana) dari diphyllobothroid cacing pita yang termasuk dalam genus Spirometra sp.. Ankilostomiosis adalah penyakit zoonosis pada anjing dan kucing yang disebabkan oleh parasit yang termasuk dalam genus Ancylostoma sp.. Kucing kasus adalah seekor kucing lokal jantan berusia 3,5 tahun dengan bobot badan 4,1 kg. Pemilik mengeluhkan kucingnya kurang aktif dan tinja agak lunak serta belum pernah diberi obat cacing. Feses kucing kasus konsistensinya agak lunak, dengan skor 3,5. Pada pemeriksaan feses dengan metode natif ditemukan telur Spirometra sp. dan Ancylostoma sp.. Kucing kasus didiagnosis menderita sparganosis disertai ankilostomiosis. Kucing kasus diobati dengan menggunakan Caniverm® kaplet sebanyak 1/2 kaplet diberikan secara peroral dua kali dengan selang waktu tujuh hari. Evaluasi kucing setelah 10 hari pascaterapi terjadi perubahan feses dari skor 3,5 ke skor 2 terbentuk dengan baik dan tidak meninggalkan bekas saat diambil. Setelah 10 hari pascapengobatan dilakukan pemeriksaan feses dengan metode natif tidak ditemukan telur cacing. Berdasarkan anamnesis, pemeriksaan klinis dan laboratorium dapat disimpulkan bahwa kucing kasus mengalami sparganosis disertai ankilostomiosis dengan prognosis fausta. Terapi kausatif dengan Caniverm® menunjukkan hasil yang baik. Pemeriksaan laboratorium pendukung lainnya yaitu hitung darah lengkap, yang menunjukkan bahwa kucing kasus tersebut mengalami trombositopenia. Dalam kasus ini tidak diberikan obat steroid yang membuat sistem kekebalan tubuh berhenti menyerang dan menghancurkan platelet, karena trombositopenia ringan bisa sembuh dengan sendirinya. Tujuan penulisan laporan kasus ini adalah untuk mendiagnosis dan mengetahui efektivitas pengobatan pada kucing lokal yang mengalami penyakit sparganosis dan ankilostomiosis.

Laporan Kasus: Penanganan Koinfeksi Skabiosis serta Toksokariosis dengan Sabun Belerang dan Pyrantel Pamoat pada Anak Kucing baskaradwaja, i gede mardawa; Batan, I Wayan; Erawan, I Gusti Made Krisna
Indonesia Medicus Veterinus Vol 12 (3) 2023
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2023.12.3.462

Koinfeksi skabiosis dan toksokariosis terjadi pada anak kucing. Skabiosis merupakan penyakit kulit menular yang disebabkan oleh tungau Sarcoptes scabiei atau Notoedres cati pada kucing. Toksokariosis yang terjadi pada kucing disebabkan oleh infeksi cacing Toxocara cati. Cacing T. cati adalah kelompok cacing nematoda yang sering ditemukan pada usus kucing. Hewan kasus adalah kucing lokal betina, berumur 2,5 bulan, dengan berat 0,73 kg. Kucing kasus mengalami perut membuncit (distensi abdomen), telinga teramati ada keropeng, kucing pasif, dan konsistensi fesesnya seperti pasta berwarna kuning kecoklatan. Pemeriksaan kerokan kulit (skin scrapping) pada telinga yang mengalami keropeng menunjukkan adanya tungau S. scabiei. Pada pemeriksaan feses dengan metode natif ditemukan telur cacing T. cati. Pemeriksaan penunjanng berupa hematologi rutin menunjukkan hewan kasus mengalami leukositosis dan anemia makrositik hipokromik ditandai dengan penurunan hemoglobin 8,3 (9,5-15,3 g/dL), penurunan MCHC 27,3 (13-21 g/dL), dan peningkatan MCV 54,2 (39-55 fL). Berdasarkan serangkaian pemeriksaan yang telah dilakukan, hewan kasus didiagnosis menderita penyakit skabiosis dan toksokariosis. Terapi yang diberikan adalah pengobatan antihelmitik menggunakan pyrantel pamoat dengan dosis 5 mg/kg bb secaraPO. Pengobatan suportif menggunakan vitamin B12 dan zat besi (Livron B-Plex® PT. Phapros Semarang, Indonesia) dengan dosis 1 tablet/ekor satu kali sehari secara peroral (PO). Pengobatan suportif menggunakan vitamin B12 dan zat besi dengan dosis 1 tablet/ekor satu kali sehari secara peroral (PO) selama 10 hari. Kucing kasus dimandikan tiga hari sekali dengan menggunakan sabun belerang. Setelah 14 hari pengobatan kucing kasus dinyatakan sembuh secara klinis.

Co-Authors Achoiro Wati Rasid Aditya Pratanto Aida Lousie Tenden Rompis Amir, Kiki Lestari Anak Agung Ayu Mirah Adi Anak Agung Sagung Istri Pradnyantari Anak Agung Sagung Kendran Anggung Praing, Umbu Yabu arsa, kadek adya Bambang Sumiarto baskaradwaja, i gede mardawa Bravanasta Glory Rahmadyasti Utomo Burhan, Haris Calista, Ruli Mauludina Djaya Putri Coornelia, Gledys Denny Widaya Lukman Deva Mutiara Giri Putri Dewi, I Dewa Ayu Dian Sasmita Dhika, I Gede Abijana Satya Diana Mustikawati Distira, Luh Ayu Yasendra Duarsa, Bima Satya Agung Dumayanti, Jeanni DWI SURYANTO Ekklesia Prasetya Emy Sapta Budiari Erika Erika Evi Marieti Hutagalung Gede Herdian Permana Putra Hasanah, Putri Nur I Gede Soma I Gusti Agung Ayu Suartini I Gusti Agung Gede Putra Pemayun I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Ketut Suada I Made Kardena I Made Kerta Pratama I Made Sukada I MADE SUMA ANTARA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Cahyadi Putra, I Putu Cahyadi I Putu Gede Yudhi Arjentinia I Wayan Batan I Wayan Puspa Ari Laxmi I Wayan Suardana I Wayan Suardana I Wayan Wirata I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Tjahajati Iwan Harjono Utama Iwan Haryono Utama Jamhari Jamhari Kadek Karang Agustina Ketut Berata Komang Andika Purnama Kristiawan, Vicky Kurniawati, Ni Made Ayu Lopes, Yoseph Adedoni Tola Luh Dewi Anggreni Luh Made Sudimartini M.Cs S.Kom I Made Agus Wirawan . M.D. Rudyanto Madania, Reydanisa Noor Made Suma Anthara Mesquita, Nelviana Mochammad Imron Awalludin Ni Luh Eka Setiasih Nirhayu, Nirhayu Nurmayani, Seli Permatasari, Serly Nur Indah Pradnyani, Gusti Ayu Putu Indira Pratama, I Gde Andhika Putra Pratiwi, Rizki Purwaka Putra, Putu Adi Guna Purwitasari, Made Santi Puspaeni, Ni Ketut Juni Putu Ayu Sisyawati Putriningsih Putu Ayu Sisyawati Putriningsih Putu Devi Jayanti Puveanthan Nagappan Govendan Qutrotu ain, Salsabila Raden Wisnu Nurcahyo Rukisti, Eniza Sembiring, Messy Saputri Senja, Naomi Orima Sibang, I Nengah Anom Adi Nugraha Sibarani, Oktryna Hodesi Slamet Raharjo Sri Kayati Widiastuti, Sri Kayati Sri Kayati Widyastuti Steven Dwi Purbantoro Sugiyarto - T. Sari Nindia Takariyanti, Dzikri Nurma'rifah Tyas Pandieka Yoga Wiadnyani, Kadek Ayu Widya Asmara Widyanti, Agnes Indah Wisnu Nurcahyo Yedija Putra Kusuma Wardana Rumbay Yoshihiro Hayashi Zefanya Christiani

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