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Mahfut
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1 Bandar Lampung, Lampung 35144
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Variation Symptoms and Resistance Response of Different Types on Orchids (Orchidaceae) Against Odontoglossum ringspot virus (ORSV) Infection Mahfut, Mahfut; Daryono, Budi Setiadi
International Series on Interdisciplinary Science and Technology Vol. 4 No. 2 (2019)
Publisher : Universitas Lampung

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Abstract

Odontoglossum ringspot virus (ORSV) is one of the viruses that are reported to infect the most orchids in Indonesia. Viral infections cause economic losses. This research was conducted to study the introduction of symptoms of infection that appear as the initial stages of disease control. The source of the virus is orchid leaves Phalaenopsis amabilis positively infected by ORSV collected from Magelang. Virus isolation was carried out by mechanical inoculation to indicator plants (Chenopodium amaranticolor and Nicotiana tabaccum). Furthermore, the results of inoculation were multiplied using various types of orchids (Dendrobium, Cymbidium, Chattleya, Phalaenopsis, Spatthoglotis, Liparis, and Pecteilis). Virus detection on orchid tissue was done trough the DAS-ELISA and observation of virus structure using TEM. The results showed that Pectelis sussanae (L.) Raf. was the only orchid plant that was immune against ORSV approached. Dendrobium sp., Cymbidium sp., Chattleya sp., Phalaenopsis sp., and Spathoglotis sp. showed a response of susceptible of ORSV severe infection symptoms, while Liparis sp. were resistant. The variation of resistance from several orchid plant against infection ORSV showed severe symptoms with the incubation periode was seen earlier. The observation of virus structure using TEM showed rigid road shape particle, 300 x 18 nm in size, which is general characteristic of Tobamovirus. This indicate an infection of ORSV is a dangerous disease and require serious control
Pengaruh Ekstrak Batang Bandotan (Ageratum conyzoides L.) Terhadap Pertumbuhan Fusarium oxysporum yang Menginfeksi Tanaman Cabai Merah Besar (Capsicum annuum L.) Fadhilla, Nurul; Agustrina, Rochmah; Chrisnawati, Lili; Mahfut, Mahfut
Konservasi Hayati Vol 19 No 2 (2023): OKTOBER
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i2.26597

Abstract

Fusarium oxysporum is a fungal pathogen that causes plant wilt which infects many horticultural crops, including red chili (Capsicum annum L.). The continual control of pathogenic fungus with synthetic pesticides can have a negative impact on the environment, so it is necessary to look for safe and environmentally friendly alternative pathogen control methods, one of which is by using natural pesticides. Bandotan (Ageratum conyzoides L.) is known as a weed, containing secondary metabolites with antifungal activity. This study aims to determine the effect of bandotan stem extract on the growth of Fusarium oxysporum in infected chili plants during the germination phase. The study was conducted using a Completely Randomized Design (CRD) with one treatment factor, namely the method of giving bandotan extract consisting of 5 replications. ANOVA results at α = 5% showed that 65% dry bandotan stem extract did not reduce Fusarium oxysporum growth on the red chili plants.
Pelatihan Analisis Genomik Melalui Pengenalan dan Penggunaan Peralatan di Laboratorium Biomolekuler, Universitas Lampung Mahfut, Mahfut; Wandono, Petrus Tri Aji; Ismail, Ikhwan; Anbiya, Lulu; Arsitalia, Metari; Sari, V. Dwi Anggita; Asadudin, David
Jurnal SOLMA Vol. 14 No. 2 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i2.18363

Abstract

Pendahuluan: Kemajuan biologi molekuler membuka peluang besar dalam riset genomik, berdampak luas pada bidang kesehatan, pertanian, dan lingkungan. Diperlukan peningkatan keterampilan sivitas akademika dalam pengoperasian alat laboratorium biomolekuler. Metode: Pelatihan dilakukan melalui pendekatan teori dan praktik langsung (hands-on training), dengan evaluasi menggunakan pre-test dan post-test untuk mengukur peningkatan pemahaman peserta. Hasil: Terdapat peningkatan signifikan pada skor post-test, terutama dalam penerapan alat untuk riset. Peserta menunjukkan peningkatan pemahaman, khususnya dalam aspek teknis penggunaan alat. Kesimpulan: Pelatihan ini efektif dalam meningkatkan kompetensi laboratorium peserta. Direkomendasikan pelatihan lanjutan dan pendampingan rutin untuk memperkuat keterampilan riset biologi molekuler secara berkelanjutan.
Pengaruh Ekstrak Tauge Dan Atonik Dalam Meningkatkan Pertumbuhan Tanaman Cabai Rawit (Capsicum frutescens L.) Insani, Mutiara; Nurcayani, Endang; Mahfut; Wahyuningsih, Sri
BULLET : Jurnal Multidisiplin Ilmu Vol. 4 No. 4 (2025): BULLET : Jurnal Multidisiplin Ilmu (INPRESS)
Publisher : CV. Multi Kreasi Media

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Abstract

Cayenne pepper (Capsicum frutescens L.) is a shrub plant of the Solanaceae family that is famous for its spicy taste and high demand in Indonesia. However, the growth of cayenne pepper plants requires optimal plant nutrients. One way to increase plant growth is through fertilization, either with organic fertilizer in the form of bean sprout extract (Vigna radiata L.) or the provision of Growth Regulators (ZPT) in the form of atonik. This study aims to determine the effect of bean sprout extract and atony on the growth of cayenne pepper, determine the most effective concentration of bean sprout extract and atony, and identify the interaction between the two. The research was conducted from February to April 2024 at the Botany Laboratory of FMIPA, University of Lampung, using a completely randomized design (CRD) with a factorial design. The first factor was the application of bean sprout extract with three concentrations (0%, 5%, and 10%), while the second factor was the application of atonic solution with two concentrations (0% and 10%). Each treatment was repeated five times, resulting in 30 experimental units. Parameters observed included plant height, root length, chlorophyll content, leaf visualization, fresh weight, dry weight, and relative water content. The data obtained were analyzed using Analysis of Variance (ANOVA) and Tukey's further test at the 5% level. The results showed that the application of 5% bean sprout extract and 0% atonik gave the best results in plant height, root length, and relative water content. The interaction between bean sprout extract and atony had an effect on the growth of cayenne pepper, but did not have a significant effect on chlorophyll content.
Co-Authors Admi Syarif Alfina Amrani Alhuda Niftakul Ahyar Alir, Rahmat Fajrin Amanto Amanto Amnah, Alifah Zul Anbiya, Lulu Anggi Anggreiny Arie Setya Putra Armalia, Inayah Arsitalia, Metari Asadudin, David Asriah Nurdini M. Asrini Puspitasari Bambang Irawan Bambang Irawan /E21207042 Budi Setiadi Daryono Budi Setiadi Daryono BUDI SETIADI DARYONO Cut Muthiadin Della Apriyanti Derlian Ella Tamara Desti Deria Rahmadani Dian Alfiah Dwi Septiani Eka Nuraini Tohari Endah Susiyanti Endang Nurcahyani Endang Nurcahyani Eti Ernawati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fadhilla, Nurul Fania Nur Izzati Fania Nur Izzati Febriansyah, Muhammad Fiska Humaizah Hajrah Hajrah, Hajrah Hasriadi Mat Akin Hasti Maulidya Fassya Heni Erlita Sari Heni Erlita Sari Heningtyas, Yunda Heri Satria Hermawan, Isna Ainun Insani, Mutiara Intan Poespita Windiyani Irni Yuni Minarni Ismail, Ikhwan Isna Rasdianah Aziz Kamisah Delilawati Pandiangan Kendari, Putri Khoirul Umur Kishy Dhea Herlanda Krisna Rendi Awalludin Kurnia Muludi Leni Agustin Lili Chrisnawati Lili Chrisnawati Lili Chrisnawati, Lili Lis Andriani Lulu Anbiya Lulut Dwi Sulistyaningsih M Kenedi M Said Hasibuan Mai Sari Mailinda Angraeni Marinda Sari Sofiyana Marjunus, Roniyus Maulidya Ananda Metari Arsitalia Mitha Valentina Treesya Panjaitan Mitha Valentina Treesya Panjaitan Mochammad Imron Awalludin Muh. Khalifah Mustami Muhammad Irfan Ardiansyah Muslimin Muslimin Nabela H.N. Harfiani Nur Qolbi, Hana Nurcayani, Endang Nurul Fadhilla Putri Kendari Rochmah Agustina Rochmah Agustrina Rochmah Agustrina RR. Ella Evrita Hestiandari Sahira Josy Arifannisa Sarah Sarah Sari, Heni Erlita Sari, Mai Sari, V. Dwi Anggita Sembiring, Rinawati Sijabat, Veronica Elizabeth Siti Zubaidah Sri Ratna Sulistiyanti Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Suhara, . Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Suryafly, Fanny Dhea Susamto Somowiyarjo Susamto Somowiyarjo Susiyanti, Endah Tengku Riza Zarzani N Treesya Panjaitan, Mitha Valentina Tri Joko Tripeni Handayani, Tundjung Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani, Tundjung Tripeni Upreti, Brij Mohan Valentina Dwi Anggita Sari WAHYUDI Wandono, Petrus Tri Aji Widi Aryani WOLLY CANDRAMILA Yulia Rahma Syari Yulia Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zelfi Julita Dwi Putri