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Mahfut
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1 Bandar Lampung, Lampung 35144
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Journal : Bioscience

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Study of orchid resistance induced by Ceratorhiza sp. against ORSV infection based on peroxidase activity Anggi Anggreiny; Tundjung Tripeni Handayani; Mahfut Mahfut
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152112960-0-00

Abstract

Orchid is one of the popular ornamental plants that widely grown in Indonesia. However, the process of orchid cultivation is often hampered by virus infections. The virus that often infects the orchids is Odontoglossum ringspot virus. Viruses that enter orchid cells and replicate will activate the orchid’s defense response. This defense response is characterized by the increase of peroxidase activity. The peroxidase enzyme works in the formation of lignin to thicken cell walls and prevent viruses from entering other cells. In addition, the orchid defense response can also be activated through induced systemic resistance by inoculation of Ceratorhiza sp. as endophytic mycorrhizae. In this study, Factorial Complete Randomized Design (CRD) was used with 2 factors. Factor 1 is the type of orchid (Phalaenopsis amabilis and Dendrobium discolor) and factor 2 is the type of treatment (inoculation of mycorrhizae, virus, and mycorrhizae-virus). The orchid’s resistance level is determined by the analysis results of peroxidase activity using spectrophotometer. The results obtained indicate that all treatment combinations strongly influence the increase of peroxidase activity. Peroxidase activity of Phalaenopsis amabilis is 1.42 [(Umg) / min] and Dendrobium discolor is 1.64 [(Umg) / min] in average. Peroxidase activity on Dendrobium discolor was higher than on Phalaenopsis amabilis. This indicates that Dendrobium discolor has a higher level of resistance when compared to Phalaenopsis amabilis.Proses budidaya anggrek seringkali terkendala oleh infeksi virus. Adapun virus yang paling banyak menginfeksi anggrek adalah ORSV. Virus yang masuk ke dalam sel anggrek dan bereplikasi akan mengaktifkan respon pertahanan anggrek. Respon pertahanan ini ditandai dengan peningkatan aktivitas peroksidase. Respon pertahanan anggrek juga dapat diaktifkan melalui induksi resistensi sistemik dengan inokulasi Ceratorhiza sp. sebagai mikoriza endofit. Dalam penelitian ini digunakan Rancangan Acak Lengkap (RAL) Faktorial dengan 2 faktor. Faktor pertama adalah jenis anggrek (Phalaenopsis amabilis dan Dendrobium discolor) dan faktor kedua adalah jenis perlakuan (inokulasi mikoriza, virus, dan mikoriza virus). Tingkat ketahanan anggrek ditentukan dari hasil analisis aktivitas peroksidase menggunakan spektrofotometer. Hasil yang diperoleh menunjukkan bahwa semua kombinasi perlakuan sangat berpengaruh terhadap peningkatan aktivitas peroksidase. Aktivitas peroksidase Phalaenopsis amabilis rata-rata 1,42 [(U/mg)/ menit] dan Dendrobium discolor 1,64 [(U/mg)/ menit]. Aktivitas peroksidase pada Dendrobium discolor lebih tinggi dibandingkan dengan Phalaenopsis amabilis. Hal ini menunjukkan bahwa Dendrobium discolor memiliki tingkat ketahanan yang lebih tinggi jika dibandingkan dengan Phalaenopsis amabilis.
Variation of response induced by ceratorhiza on Dendrobium discolor and Phalaenopsis amabilis to odontoglossum ringspot virus infection based on disease intensity and plant resistance level Mahfut Mahfut; Mitha valentina treesya Panjaitan; Tundjung Tripeni Handayani; Sri Wahyuningsih
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261114027-0-00

Abstract

The popular cultivated orchid is Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are very important for orchid plants to complete their life cycle. Mycorrhizae are capable of symbiosis with the root system of the host plant which can increase the absorption of these elements. The purpose of this study was to find out the symptoms of infection, the intensity of infectious diseases, the level of resistance to infection. The method used in this study was completely randomized design (CRD) with 2 factors and 4 iterations, the first factor was the type of orchid used Phalaenopsis amabilis (A1) and Dendrobium discolor (A2), and the second factor was the type of administration of mycorrhizal., Virus , and mycorrhizal virus. Observation of the symptoms of the disease is done by looking at the symptoms that appear on the leaves that have been infected with ORSV, while to determine the intensity of the disease and the level of resistance can use a predetermined formula. The results showed that the disease intensity in Phalaenopsis amabilis was more severe than in Dendrobium discolor, which was 40% on each leaf. The level of resistance in Dendrobium discolor is more resistant than Phalaenopsis amabilis, this indicates that Dendrobium discolor on average has a tolerant response. Ceratorhiza could not suppress the intensity of the disease, because from the results obtained, treatment of orchids inoculated with mycorrhiza and virus showed more severe symptoms than treatment only inoculated with virus. Ceratorhiza was unable to increase the resistance of Phalaenopsis amabilis and Dendrobium discolor, this was because treatment of orchids inoculated with mycorrhiza and virus showed a very simple response compared to treatmentonly inoculated with virus.Anggrek populer dibudidayakan adalah Phalaenopsis sp. dan Dendrobium sp. Mikoriza sangat penting bagi tanaman anggrek untuk melengkapi siklus hidupnya. Mikoriza  mampu bersimbiosis dengan sistem akar tanaman inang yang dapat meningkatkan daya serap unsur tersebut. Tujuan penelitian untuk mengetahui gejala infeksi, intensitas penyakit menular, tingkat resistensi terhadap infeksi. Metode penelitian: Rancangan Acak Lengkap (RAL) dengan 2 faktor dan 4 iterasi, faktor pertama adalah jenis anggrek yang digunakan Phalaenopsis amabilis (A1) dan Dendrobium discolor (A2), dan faktor kedua adalah jenis pemberian mikoriza, virus, dan virus mikoriza. Pengamatan gejala penyakit dilakukan dengan melihat gejala yang muncul pada daun yang telah terinfeksi ORSV. Hasil penelitian menunjukkan intensitas penyakit pada Phalaenopsis amabilis lebih parah dibandingkan dengan Dendrobium discolor yaitu 40% pada setiap daun. Tingkat ketahanan pada Dendrobium discolor lebih tahan dibandingkan Phalaenopsis amabilis. Dendrobium discolor memiliki respon yang toleran. Ceratorhiza tidak dapat menekan intensitas penyakit. Pengobatan  anggrek yang diinokulasi dengan mikoriza dan virus menunjukkan gejala yang lebih parah daripada pengobatan yang hanya diinokulasi virus. Ceratorhiza tidak mampu meningkatkan ketahanan terhadap Phalaenopsis amabilis dan Dendrobium discolor, hal ini dikarenakan perlakuan anggrek yang diinokulasi mikoriza dan virus menunjukkan respon yang sangat sederhana dibandingkan dengan perlakuan yang hanya diinokulasi virus.
Co-Authors Admi Syarif Agus Rahardi, Agus Alhuda Niftakul Ahyar Alir, Rahmat Fajrin Amanto Amanto Amanto Amanto Amnah, Alifah Zul Amrani, Alfina Anbiya, Lulu Anggi Anggreiny Apriyanti, Della Arie Setya Putra Armalia, Inayah Arsitalia, Metari Asadudin, David Asmanto, Budi Asriah Nurdini M. Asrini Puspitasari Bambang Irawan Bambang Irawan /E21207042 Budi Setiadi Daryono Budi Setiadi Daryono BUDI SETIADI DARYONO Chrisnawati , Lili Cut Muthiadin Della Apriyanti Derlian Ella Tamara Desti Deria Rahmadani Dian Alfiah Dian Alfiah Dwi Septiani Eka Nuraini Tohari Endah Susiyanti Endang Nurcahyani Endang Nurcahyani Eti Ernawati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fachry Abda El Rahman Fadhilla, Nurul Fania Nur Izzati Fania Nur Izzati Febriansyah, Muhammad Fiska Humaizah Hajrah Hajrah, Hajrah Harfiani, Nabela H.N. Hasriadi Mat Akin Hasti Maulidya Fassya Heni Erlita Sari Heni Erlita Sari Heningtyas, Yunda Heri Satria Heri Satria Herlanda, Kishy Dhea Hermawan, Isna Ainun Insani, Mutiara Intan Poespita Windiyani Irni Yuni Minarni Ismail, Ikhwan Isna Rasdianah Aziz Kamisah Delilawati Pandiangan Kamisah Delilawati Pandiangan Kendari, Putri Kenedi, M Kishy Dhea Herlanda Krisna Rendi Awalludin Kurnia Muludi Leni Agustin Lili Chrisnawati Lili Chrisnawati, Lili Lis Andriani Lulu Anbiya Lulut Dwi Sulistyaningsih M Said Hasibuan Mai Sari Mailinda Angraeni Marinda Sari Sofiyana Marjunus, Roniyus Maulidya Ananda Metari Arsitalia Mitha Valentina Treesya Panjaitan Mitha Valentina Treesya Panjaitan Mochammad Imron Awalludin Muh. Khalifah Mustami Muhammad Irfan Ardiansyah Muslimin Muslimin Nur Qolbi, Hana Nurcayani, Endang Nurul Fadhilla Putri Kendari Raden Mohamad Herdian Bhakti Rahmadani, Desti Deria Rochmah Agustina Rochmah Agustrina Rochmah Agustrina RR. Ella Evrita Hestiandari Sahira Josy Arifannisa Salsabila, Diana Sarah Sarah Sari, Heni Erlita Sari, Mai Sari, V. Dwi Anggita Sembiring, Rinawati Sijabat, Veronica Elizabeth Siti Zubaidah Sri Ratna Sulistiyanti Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Suhara, . Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Suryafly, Fanny Dhea Susamto Somowiyarjo Susamto Somowiyarjo Susiyanti, Endah Syamsiar, Syamsiar Tamara, Derlian Ella Tengku Riza Zarzani N Treesya Panjaitan, Mitha Valentina Tri Joko Tripeni Handayani, Tundjung Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani, Tundjung Tripeni Umur, Khoirul Upreti, Brij Mohan Valentina Dwi Anggita Sari WAHYUDI Wandono, Petrus Tri Aji Widi Aryani WOLLY CANDRAMILA Yulia, Yulia Rahma Syari Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zelfi Julita Dwi Putri