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Variation of resistance response of orchids induced by Rhizoctonia against the infection of Odontoglossum ringspot virus (ORSV) based on percentage of disease development Fania Nur Izzati; Mahfut Mahfut; Eti Ernawiati; Sri Wahyuningsih
Journal of Sustainability Science and Technology Vol. 1 No. 1 (2021)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v1i1.3

Abstract

Orchid is one of the largest groups of flowering plants that are in great demand by society. Besides, the various flowers with a long vase life, the price of orchids is also stable and affordable. Phalaenopsis and Dendrobium are the most popular types of orchids. To anticipate this, orchid cultivation needs to be improved. However, orchid cultivation is not always accompanied by the effort of disease prevention, particularly those caused by viruses. The virus that most infects orchids is the Odontoglossum ringspot virus (ORSV). Symptoms that appear on the leaves are chlorosis, mosaic with a pattern of lines, circles, and necrotic rings/rings. Control of viral infections can utilize organisms that are able to induce resistance, namely mycorrhizae. Mycorrhizae will penetrate into the root cortex tissue and provide nutrients for the orchid. Mycorrhizae that are quite often found are Rhizoctonia and are applied by induction to orchids. Induction was undertaken by planting orchids in Rhizoctonia inoculum until anatomically a peloton structure (solid coils) was formed on the orchid roots. The aim of this study was to study the response of orchid plants in the form of percentage incidence and intensity of the disease and the level of plant resistance to ORSV infection in Phalaenopsis amabilis and Dendrobium discolour which had been induced by Rhizoctonia. This research was conducted at the Laboratory of Botany, Biology, FMIPA, Universitas Lampung, using a factorial completely randomized design (CRD). Factor 1 was the type of orchid and factor 2 was mycorrhizal induction, virus inoculation, and a combination of both with 4 replications. The finding indicated that Phalaenopsis amabilis was more susceptible to ORSV infection than Dendrobium discolour based on a higher incidence and intensity of the disease. Mycorrhizal induction and virus inoculation (MAV) in Dendrobium discolour showed an increase of resistance response compared to Phalaenopsis amabilis.
Induction of Rhizoctonia resistance to odontoglossum ringspot virus (ORSV) infection on orchid leaf organ viability Eka Nuraini Tohari; Mahfut Mahfut; Tundjung Tripeni Handayani; Sri Wahyuningsih
Journal of Sustainability Science and Technology Vol. 1 No. 2 (2021)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v1i2.4

Abstract

Orchid plants are in great demand by the public as ornamental plants that have economic value and the beauty of attractive flower shapes and colors, especially Phalaenopsis and Dendrobium. Related to this, conservation efforts are needed through cultivation. However, these efforts are often hampered by viral infections. One of the viruses that trigger inhibition of orchid cultivation that often appears is ORSV. Control efforts that are relatively safe and efficient can utilize mycorrhizae in orchids which play a role in increasing the absorption of plant nutrients. The use of Phalaenopsis and Dendrobium with the induction of Rhizoctonia sp. against ORSV is a very important alternative biofertilizer and biocontrol agent. The objectives of this study were 1) to determine the effectiveness of orchids induced by Rhizoctonia resistance, 2) to determine the viability of orchid leaf organs to ORSV infection, 3) to determine the viability of orchid root organs resulting from Rhizoctonia resistance to ORSV infection. This study was conducted in January 2021 – March 2021 at the Botanical 2 Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This study employed a completely randomized factorial with 6 treatments and 4 replications. The variable observed in this study was the effectiveness of mycorrhizae, leaf and root length, number of leaves and roots, and leaf width. The data obtained were homogenized using Levene's test and then analyzed by ANOVA and Tukey's further test at the 5% level. The results showed that the effectiveness of mycorrhizae occurred on the 3rd day of incubation. In leaf organs, Phalaenopsis amabilis showed an increased response and Dendrobium discolour showed a decreased response in leaf length, width, and number. Meanwhile, in the root organ, Dendrobium discolour showed an increased response and Phalaenopsis amabilis showed a decreased response in root length and number of roots.
Various ORSV infection symptoms on ceratorhiza induction in orchid plants Mitha Valentina Treesya Panjaitan; Mahfut Mahfut; Tundjung Tripeni Handayani; Sri Wahyuningsih
Journal of Sustainability Science and Technology Vol. 1 No. 2 (2021)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v1i2.5

Abstract

Several types of orchids that are in great demand and cultivated by various groups are Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are needed by orchid plants to complete their life cycle. This is because mycorrhizae are capable of symbiosis with the root system of the host plant so that the plant's capacity to absorb elements will increase. This study aims to determine the extent to which Ceratorhiza induction on Phalaenopsis amabilis and Dendrobium discolour orchids can suppress the symptoms of Odontoglossum ringspot virus (ORSV) infection. The method used in this study was a factorial completely randomized design (CRD) with 2 factors and 4 replications. The first factor is the type of orchid used, namely Phalaenopsis amabilis (A1) and Dendrobium discolour (A2), while the second factor is the type of administration of mycorrhizae, viruses, and mycorrhizal viruses. Observation of disease symptoms is done by looking at the symptoms that appear on leaves that have been infected with ORSV. The results showed that the symptoms that appeared in Phalaenopsis amabilis were in the form of mosaic, necrosis, leaf malformation, while in Dendrobium discolour were mosaic and necrotic. Ceratorhiza was not able to suppress the symptoms of disease infection because based on the results obtained; the treatment on orchids inoculated with mycorrhizae and viruses showed more severe symptoms compared to the treatment only inoculated with viruses increased response and Dendrobium discolours showed a decreased response in leaf length, width, and number. Meanwhile, in the root organ, Dendrobium discolour showed an increased response and Phalaenopsis amabilis showed a decreased response in root length and number of roots.
Bedah Buku Ajar Biologi Kelas XII SMA pada Materi Genetika: Upaya Peningkatan Kompetensi Materi Pada Guru dan Calon Guru Biologi Wolly Candramila; Suhara Suhara; Asriah Nurdini Mardiyyaningsih; Siti Zubaidah; Mahfut Mahfut; Marinda Sari Sofiana
Jurnal Pengabdi Vol 6, No 1 (2023): April 2023
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jplp2km.v6i1.59262

Abstract

Kajian tentang miskonsepsi yang ditemukan pada buku ajar pernah dilaporkan dalam berbagai penelitian. Ajang pertemuan ilmiah yang diikuti oleh guru dan calon guru bisa mendukung peningkatan kompetensi keilmuan sehingga bisa memperkuat pengetahuan yang diperlukan untuk menyaring buku ajar yang baik untuk digunakan dalam pembelajarannya. Kegiatan pengabdian ini menggunakan bentuk bedah buku pada materi genetika dalam tiga buku Biologi untuk siswa di kelas XII SMA yang banyak digunakan di Kota Pontianak menurut hasil survei di 27 SMA pada semester gazal TA 2021/2022.  Aspek yang dikaji dari ketiga buku ajar meliputi 1) kesesuaian dengan kurikulum dan kompetensi dasar, 2) urutan/ sistematika dalam memahami konsep, 3) konten/ kebenaran konsep, 4) tata bahasa dan penulisan, 5) tampilan dan kemenarikan, dan 6) tuntutan dalam soal latihan. Kegiatan ini diikuti oleh 34 peserta yang terdiri atas 6 orang dosen, 16 guru, 11 mahasiswa, dan 1 umum. Pemaparan materi berjalan dengan lancar oleh keempat narasumber. Peserta menilai bahwa kegiatan ini penting untuk menggali pengetahuan yang lebih dalam dan lebih luas dalam memahami konsep pada materi genetika yang sebenarnya.
Peningkatan Kualitas Pemahaman Konten dan Teknik Pengajaran Mata Pelajaran Fisika, Kimia, Matematika, Biologi, dan Teknologi Informasi Komputer (TIK) Roniyus Marjunus; Kamisah Delilawati Pandiangan; Amanto Amanto; Mahfut Mahfut; Yunda Heningtyas; Heri Satria
Jurnal SOLMA Vol. 12 No. 1 (2023)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v12i1.10201

Abstract

Background: Pembelajaran Fisika, Kimia, Matematika, Biologi dan Teknologi Informasi-Komputer (TIK) atau disingkat MIPA-TIK di level Sekolah Menengah Atas (SMA) merupakan salah satu bagian dari komponen pendidikan tersebut. Pemahaman yang baik dan benar dari siswa SMA tentang MIPA-TIK diperlukan agar siswa SMA tidak lagi memandang mata pelajaran tersebut sebagai ilmu pengetahuan yang sukar. Oleh karena itu tingkat kualitas pemahaman guru-guru mata pelajaran tersebut di Provinsi Lampung umumnya dan Kabupaten Lampung Selatan khususnya, memegang peranan yang sangat penting. Metode: Evaluasi dilakukan pada awal kegiatan (pre-test) dan akhir kegiatan (post-test) untuk melihat keberhasilan dari kegiatan ini. Hasil: Hasil post-test dibanding pre-test sebesar 45,8%. Kesimpulan: terjadi peningkatan kemampuan atau kompetensi guru-guru dalam menyampaikan materi mata pelajaran tersebut secara baik dan benar.
POTENSI DAN ANALISA BIAYA PENYEDIAAN BIBIT TEBU (Saccharum officinarum L.) UNGGUL SECARA IN VITRO DI PT. GUNUNG MADU PLANTATIONS Heni Erlita Sari; Mahfut Mahfut; Alhuda Niftakul Ahyar
Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 5, No 1 (2022): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v5i1.1313

Abstract

Sugarcane productivity included cane yield and sugar extracted rate (rendemen) fluctuated annually. The main causes of low sugarcane productivity is quality of planting material with no high sugar extracted rate (sugar rendemen) potential, resistance to pests and diseases, stability production, tolerance to climate changed and early age of harvest. For increasing of the yield productivity and producing a superior sugarcane planting material with competitiveness cost, the vegetative propagation through in vitro culture technique via shoot tip culture was carried out. The objective of this research is to produce a superior sugarcane planting in vitro and analyze the costs required. The research was carried out at the Tissue Culture Laboratory, PT. Gunung Madu Plantations (GMP) in March-May 2021. The research stages started from the preparation of explant source plants, sterilization of explants and tools, manufacture of stock solutions and planting media, subculture, and analysis of the costs needed to provide superior sugarcane seeds. The results showed that the basal swelling of explants, increasing the number of shoots, root elongation, and the method of providing superior sugarcane seeds from apical shoot explants in vitro were relatively more competitive with the price of plantlets per culture tube contained 10 clones costly were Rp. 20,328, compared with conventional propagation methods. with the price of sugar cane seedlings material were Rp 1,603/stem.
ANALISIS KLOROFIL DAN PERTUMBUHAN EKSPLAN KACANG KEDELAI (Glycine max (L.) Merr.) KULTIVAR ANJASMORO SECARA In Vitro DENGAN PEMBERIAN AIR KELAPA (Cocos nucifera L.) Endang Nurcahyani; Della Apriyanti; Sri Wahyuningsih; Mahfut Mahfut
Analit: Analytical and Environmental Chemistry Vol 5, No 2 (2020): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.81 KB) | DOI: 10.23960/aec.v5i2.2020.p101-110

Abstract

Perbanyakan kacang kedelai (Glycine max L.) dengan menggunakan teknik kultur jaringan dapat membantu memperbanyak tanaman dengan menghasilkan biji yang mempunyai keunggulan, antara lain mampu menghasilkan biji dalam jumlah yang besar dengan waktu yang singkat dan tidak membutuhkan tempat yang luas, kesehatan dan mutu biji lebih terjamin serta kecepatan tumbuh biji lebih cepat. Penelitian ini menggunakan medium Murashige and Skoog (MS) dengan pemberian air kelapa. Penelitian ini bertujuan untuk mengetahui efek pemberian air kelapa terhadap jumlah planlet yang hidup serta untuk mengetahui kandungan klorofil a, klorofil b, dan klorofil total optimum pada planlet kacang kedelai kultivar anjasmoro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu konsentrasi air kelapa dengan 4 taraf perlakuan: 0%, 5%, 10%, dan 15% dengan 6 ulangan. Data dianalisis dengan menggunakan uji Levene dan uji lanjut dengan Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil penelitian menunjukkan bahwa hasil persentase planlet yang hidup menunjukkan hasil 100% hidup, dan pemberian air kelapa belum memberikan pengaruh terhadap kandungan  klorofil a, klorofil b, dan klorofil total.http://dx.doi.org/10.23960/aec.v5.i2.2020.p101-110 
ANALISIS KADAR KLOROFIL PADA BUNCIS (Phaseolus vulgaris L.) TERINDUKSI INDOLE ACETIC ACID (IAA) SECARA IN VITRO Endang Nurcahyani; Desti Deria Rahmadani; Sri Wahyuningsih; Mahfut Mahfut
Analit: Analytical and Environmental Chemistry Vol 5, No 1 (2020): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.3 KB) | DOI: 10.23960/aec.v5i1.2020.p15-23

Abstract

Buncis (Phaseolus vulgaris L.) adalah salah satu komoditas pangan yang memiliki sumber protein dan aman dikonsumsi.Seiring dengan berjalannya waktu dan perubahan iklim yang tidak menentu, para petani sayuran termasuk Buncis kesulitan dalam memproduksi hasil pertanian dengan baik, sehingga menyebabkan produksi tanaman buncis menurun. Indole Acetic Acid (IAA) merupakan hormon auksin yang terdapat pada ujung akar, batang dan pembentukan bunga. Hormon auksin IAA dapat memicu percepatan pertumbuhan pada akar, batang dan perkembangan bunga. Penelitian ini bertujuan untuk mengetahui efek pemberian IAA pada jumlah planlet  hidup dan kadar klorofil a, b dan total planlet buncis (Phaseolus vulgaris L.) secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan variasi konsentrasi Indole Acetic Acid  yang terdiri dari 4 taraf perlakuan yaitu 0 ppm, 0,5 ppm, 1 ppm, dan 1,5 ppm. Masing masing konsentrasi dilakukan 5 kali pengulangan dan setiap pengulangan terdiri dari 3 eksplan biji buncis dalam setiap botol kultur. Data dianalisis dengan menggunakan Uji homogenitas dan Uji Lanjut Beda Nyata Terkecil (BNT) pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa persentase planlet hidup menunjukkan 100% hidup dan pemberian IAA memberikan pengaruh terhadap kadar klorofil a dan total secara in vitro.http://dx.doi.org/10.23960/aec.v5.i1.2020.p15-23
Pendampingan Desa Devisa Melalui Budidaya Pisang Cavendish Di Desa Batanghari Ogan, Kecamatan Tegineneng, Kabupaten Pesawaran Muslimin Muslimin; Mahfut Mahfut; Lis Andriani; Fiska Humaizah
Jurnal SOLMA Vol. 12 No. 2 (2023)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v12i2.11008

Abstract

Background: Salah satu produk pertanian yang memiliki potensi pasar lokal dan internasional adalah pisang cavendish. Berdasarkan analisis situasi, Desa Batanghari Ogan, Kecamatan Tegineneng, Kabupaten Pesawaran memiliki potensi untuk pengembangan perkebunan rakyat yang variatif. Tujuan kegiatan ini adalah mengembangkan desa Batanghari Ogan sebagai desa Devisa melalui pengembangan produk pertanian berorientasi pasar. Metode: Pelatihan ini diikuti oleh 20 orang peserta dengan metode Community-Centered Development (CCD) metode yang dilakukan dalam kegiatan ini melalui pelatihan, pendampingan, dan evaluasi. Hasil: peningkatan sebesar 23%. Kenaikan tertinggi pada materi Teknik Budidaya Pisang Cavendish sebesar 26%, kemudian Perencanaan SDM Berorientasi Ekspor sebesar 20%, materi Kepabeanan dan Ekspor Impor sebesar 19%, dan Globalisasi dan Sektor Pertanian sebesar 18%. Presentase kemampuan berdasarkan gender menunjukkan peserta perempuan memiliki peningkatan kemampuan lebih tinggi yaitu 16.75% dibandingkan peserta laki-laki sebesar 2%. Kesimpulan: Hasil kegiatan ini dapat menjadi acuan dalam pengembangan Desa Batanghari Ogan menjadi desa dengan potensi produk yang berorientasi ekspor. Kelanjutan kegiatan ini masih dibutuhkan terutama pendampingan dalam hilirisasi produk. Pengembangan lebih jauh difokuskan pada mata rantai ekonomi dari produksi hingga pemasarannya.
Implementasi Aplikasi Sistem “Pakar Anggrek” Berbasis Android Di Kebun Raya Liwa Mahfut; Admi Syarif; Tundjung Tripeni Handayani; Sri Wahyuningsih; Kurnia Muludi; Muhammad Irfan Ardiansyah
AMMA : Jurnal Pengabdian Masyarakat Vol. 2 No. 7 : Agustus (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Natural orchids are one of the original flora collections in Liwa Botanical Gardens that need to be preserved. Based on the results of our previous research, it is known that some natural orchids show symptoms of disease infection. The Liwa Botanical Gardens do not yet know a safe disease infection control technique. Disease control is still using pesticides that are harmful to the environment. The results of our previous research have resulted in new methods and solutions for disease control through a web-based expert system. This method was considered very helpful for diagnosis but was considered inefficient, so the Android-based "Orchid Expert" application was developed. This Community Service Dissemination of Research Results (PKM-DHR) activity is a continuation of our previous research results. The problem-solving formulation method is carried out through direct counselling and training at the Liwa Botanical Gardens. The activity was carried out on Wednesday, August 16, 2023, with a total of 30 participants. The results of the training activities at the demonstration stage of downloading and using the application show that the accuracy of the system diagnostic results is valid, with an accuracy rate of 92.9%. This can be interpreted as meaning that this application can be applied in society. The results of this activity indicate that the Liwa Botanical Garden management understands and is able to apply the development of natural orchid disease control methods as a conservation effort. Furthermore, the Liwa Botanical Garden is also expected to be able to independently diagnose and control natural orchid disease infections.
Co-Authors Admi Syarif Agus Rahardi, Agus Alhuda Niftakul Ahyar Alir, Rahmat Fajrin Amanto Amanto Amanto Amanto Amnah, Alifah Zul Amrani, Alfina Anbiya, Lulu Anggi Anggreiny Apriyanti, Della Arie Setya Putra Armalia, Inayah Arsitalia, Metari Asadudin, David Asmanto, Budi Asriah Nurdini M. Asrini Puspitasari Bambang Irawan Bambang Irawan /E21207042 Budi Setiadi Daryono Budi Setiadi Daryono BUDI SETIADI DARYONO Chrisnawati , Lili Cut Muthiadin Della Apriyanti Derlian Ella Tamara Desti Deria Rahmadani Dian Alfiah Dian Alfiah Dwi Septiani Eka Nuraini Tohari Endah Susiyanti Endang Nurcahyani Endang Nurcahyani Eti Ernawati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fachry Abda El Rahman Fadhilla, Nurul Fania Nur Izzati Fania Nur Izzati Febriansyah, Muhammad Fiska Humaizah Hajrah Hajrah, Hajrah Harfiani, Nabela H.N. Hasriadi Mat Akin Hasti Maulidya Fassya Heni Erlita Sari Heni Erlita Sari Heningtyas, Yunda Heri Satria Heri Satria Herlanda, Kishy Dhea Hermawan, Isna Ainun Insani, Mutiara Intan Poespita Windiyani Irni Yuni Minarni Ismail, Ikhwan Isna Rasdianah Aziz Kamisah Delilawati Pandiangan Kamisah Delilawati Pandiangan Kendari, Putri Kenedi, M Kishy Dhea Herlanda Krisna Rendi Awalludin Kurnia Muludi Leni Agustin Lili Chrisnawati Lili Chrisnawati, Lili Lis Andriani Lulu Anbiya Lulut Dwi Sulistyaningsih M Said Hasibuan Mai Sari Mailinda Angraeni Marinda Sari Sofiyana Marjunus, Roniyus Maulidya Ananda Metari Arsitalia Mitha Valentina Treesya Panjaitan Mitha Valentina Treesya Panjaitan Mochammad Imron Awalludin Muh. Khalifah Mustami Muhammad Irfan Ardiansyah Muslimin Muslimin Nur Qolbi, Hana Nurcayani, Endang Nurul Fadhilla Putri Kendari Raden Mohamad Herdian Bhakti Rahmadani, Desti Deria Rochmah Agustina Rochmah Agustrina Rochmah Agustrina RR. Ella Evrita Hestiandari Sahira Josy Arifannisa Salsabila, Diana Sarah Sarah Sari, Heni Erlita Sari, Mai Sari, V. Dwi Anggita Sembiring, Rinawati Sijabat, Veronica Elizabeth Siti Zubaidah Sri Ratna Sulistiyanti Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Suhara, . Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Suryafly, Fanny Dhea Susamto Somowiyarjo Susamto Somowiyarjo Susiyanti, Endah Syamsiar, Syamsiar Tamara, Derlian Ella Tengku Riza Zarzani N Treesya Panjaitan, Mitha Valentina Tri Joko Tripeni Handayani, Tundjung Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani, Tundjung Tripeni Umur, Khoirul Upreti, Brij Mohan Valentina Dwi Anggita Sari WAHYUDI Wandono, Petrus Tri Aji Widi Aryani WOLLY CANDRAMILA Yulia, Yulia Rahma Syari Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zelfi Julita Dwi Putri