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Pengaruh Pemberian Human Wharton’s jelly mesenchymal stem cells Terhadap Gambaran Histopatologi Sel Hepatosit yang Diinduksi Aluminium Klorida Putri Amran, Fajriana Anggun; Nita Afriani; Dolly Irfandy; Eryati Darwin; Eka Nofita; Tofrizal
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 3 (2025): September 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i3.1508

Latar Belakang: Aluminium klorida (AlCl3) adalah zat hepatotoksik yang dapat ditemukan dalam air, udara, serta berbagai bahan makanan , sehingga manusia berpotensi terpapar setiap hari. Paparan kronis AlCl3 diketahui menimbulkan akumulasi pada hepar yang berujung pada kerusakan hepatosit. Gambaran histopatologi khas akibat paparan toksik ini meliputi degenerasi hidrofik, piknosis, karioreksis, kariolisis, dan nekrosis. Dengan. Human Wharton’s jelly mesenchymal stem cells (HWJ-MSCs), yang berasal dari jaringan mucoid plasenta, memiliki sifat multipotent, regenerative dan imunomodulator, sehingga berpotensi memperbaiki kerusakan hepatosit. Objektif: Penelitian ini bertujuan unutuk mengetahui pengaruh pemberian HWJ-MSCs terhadap gambaran histopatologi sel hepatosit tikus yang diinduksi aluminium klorida Metode: Penelitian eksperimental laboratorik dengan desain Post Test Only Control Group dilakukan pada 18 ekor tikus wistar jantan. Hewan dibagi menjadi tiga kelompok : kontrol negative, kontrol positif dengan induksi AlCl3 dan perlakuan dengan induksi AlCl3 ditambah pemberian HWJ-MSCs. Hepar difiksasi, diproses histologis, dan diwarnai menggunakan H&E. Presentase kerusakan hepatosit dinilai pada tiga lapangan pandang dengan pembesaran 400x Hasil: Rerata kerusakan hepatosit adalah 1,7% pada kontrol negative, 68,7% pada kontrol positif, dan 35,8% pada kelompok perlakukan. Tampak perbaikan gambaran histopatologi pada kelompok perlakuan dibanding kelompok kontrol kontrol positif. Kesimpulan: Pemberian HWJ-MSCs berpotensi menurunkan kerusakan hepatosit akibat induksi AlCl3 meskipun dosis yang diberikan belum cukup untuk mengembalikan kondisi sel hepatosit normal.

Angiofibroma Beyond the Nasopharynx: Diagnostic Challenges and Endoscopic Management of Two Atypical Cases Arising from the Ethmoid and Sphenoid Sinuses Dolly Irfandy; Bestari Jaka Budiman; Jihan Mudrika Rahmi; Auzy Yoana Khalisha; Hippocrates Kam
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 2 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i2.1500

Background: Angiofibroma is a histologically benign but locally aggressive vascular neoplasm almost exclusively associated with the nasopharynx of adolescent males (Juvenile Nasopharyngeal Angiofibroma, JNA). Extranasopharyngeal angiofibroma (ENA) is an exceptionally rare variant that originates outside the sphenopalatine foramen, posing significant diagnostic and management challenges due to its atypical locations, age of presentation, and clinical mimicry of other sinonasal pathologies. Case presentation: We present two sophisticated cases of ENA managed at our tertiary center. Case 1: A 35-year-old male presented with unilateral nasal obstruction. Endoscopy and imaging revealed a hypervascular mass centered in the posterior ethmoid sinus, destroying the basal lamella and abutting the skull base. Histopathological analysis was initially confounded by features resembling a solitary fibrous tumor (SFT), requiring a comprehensive immunohistochemical panel (IHC) including STAT6 and nuclear beta-catenin to confirm the diagnosis of angiofibroma. Case 2: A 17-year-old male presented with symptoms and imaging (non-contrast CT) highly suggestive of a benign sphenochoanal polyp. An initial attempt at routine endoscopic removal was aborted due to unexpected, profuse hemorrhage. Subsequent advanced imaging (CTA/MRI) revealed a hypervascular sphenoid-based angiofibroma. Both patients underwent preoperative superselective embolization followed by successful, purely endoscopic tumor resection with no recurrence at 12 and 18-month follow-up, respectively. Conclusion: ENA is a critical, albeit rare, diagnostic consideration for any vascular sinonasal mass, regardless of patient age or tumor location. These cases underscore the unreliability of "classic" clinical and radiological signs, the diagnostic pitfalls of histopathological mimics like SFT and polyps, and the critical role of advanced IHC (nuclear beta-catenin) for definitive diagnosis. A modern, multidisciplinary approach combining preoperative embolization with endoscopic resection offers a safe and effective pathway to cure.

Atypical Presentation of Clinically Non-Functioning Pituitary Macroadenoma: Case Report of Concurrent Graves' Disease and Multi-Axis Pituitary Hypofunction in a 40-Year-Old Female Rizkha Amaliya; Dolly Irfandy; Hesty Lidya Ningsih; Alexander Kam; Mardijas Efendi
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 6 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i6.1610

Background: Non-functioning pituitary adenomas (NFPAs) present variable clinical manifestations depending on tumor size and location. Concurrent primary autoimmune thyroid disease in NFPA patients is exceptionally rare and requires careful diagnostic distinction from secondary hypothyroidism. Case presentation: A 40-year-old female presented with progressive bilateral peripheral vision narrowing over 3 months, chronic bifrontal headaches, secondary amenorrhea, and nocturia-predominant polyuria. Neuro-ophthalmologic examination revealed bitemporal hemianopsia with visual acuity 20/200 left eye and 20/25 right eye. Endocrine evaluation demonstrated: primary hyperthyroidism (TSH 0.02 µIU/mL, FT4 28.32 pmol/L, positive thyroid receptor antibodies), secondary adrenal insufficiency (cortisol 1 µg/dL, ACTH <5 pg/mL), and hypogonadotropic hypogonadism (LH 1.62 IU/L). Neuroimaging revealed a 2.13 × 2.28 × 3.05 cm sellar/suprasellar lesion with optic chiasm compression (Knosp Grade II). Endoscopic transsphenoidal surgery achieved complete gross total resection. Histopathology confirmed null-cell adenoma with low Ki-67 proliferation index (2%). Conclusion: Postoperative course was uncomplicated with rapid visual field improvement, complete resolution of bitemporal hemianopsia by 2 weeks, and normalization of all endocrine axes by 12 weeks, including menstrual recovery and gonadal axis restoration. This case illustrates the diagnostic challenge of distinguishing secondary hyperprolactinemia from primary prolactinoma in NFPA, the rarity of concurrent Graves' disease with pituitary macroadenoma, and favorable outcomes with complete surgical decompression achieving multisystem endocrine recovery.

Association of IRF6 and rs22355371 polymorphism mutation with risk of non-syndromic orofacial cleft Al Hafiz; Benni Raymond; Hirowati Ali; Husna Yetti; Dolly Irfandy; Fauzia Latifah Supriyadi
Oto Rhino Laryngologica Indonesiana Vol. 56 No. 1 (2026): VOLUME 56, NO. 1 JANUARY - JUNE 2026
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v56i1.761

Background: Non-syndromic orofacial cleft (NSOFC) is a congenital abnormality characterized by incomplete division of the oral and nasal cavities, with no other abnormalities present. NSOFC is the result of disruptions in the normal biomolecular processes of craniofacial development. Many genes have been linked to NSOFC, one of them is IRF6 gene. Purpose: To investigate the association of IRF6 gene and incident of rs22355371 mutation, with the risk of developing NSOFC. Method: A cross-sectional comparative study, conducted in DR M Djamil General Hospital Padang Indonesia from September 2024 to November 2024 involving 13 lip or palate tissues from patient with NSOFC, and 13 maxillary crest from controls. Expression of IRF6 was examined with real time polymerase chain reaction (RT PCR) and the segment of rs22355371 was examined with Sanger sequencing. Result: IRF6 gene expression was shown to have a median value of 129.3 in NSOFC patients, and 252.5 in controls. This indicated that IRF6 gene expressions were lower in NSOFC patients. The Mann Whitney test was performed had it can be concluded that there is no statistically significant relation between IRF6 gene expression between NSOFC patients and controls with a p value = 0.174 (p > 0.05). Conclusion: Compared to controls, NSOFC patients had decreased levels of IRF6 gene expression and we found rs22355371 polymorphism either in NSOFC patient or controls.

Angiofibroma Beyond the Nasopharynx: Diagnostic Challenges and Endoscopic Management of Two Atypical Cases Arising from the Ethmoid and Sphenoid Sinuses Dolly Irfandy; Bestari Jaka Budiman; Jihan Mudrika Rahmi; Auzy Yoana Khalisha; Hippocrates Kam
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 2 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i2.1500

Background: Angiofibroma is a histologically benign but locally aggressive vascular neoplasm almost exclusively associated with the nasopharynx of adolescent males (Juvenile Nasopharyngeal Angiofibroma, JNA). Extranasopharyngeal angiofibroma (ENA) is an exceptionally rare variant that originates outside the sphenopalatine foramen, posing significant diagnostic and management challenges due to its atypical locations, age of presentation, and clinical mimicry of other sinonasal pathologies. Case presentation: We present two sophisticated cases of ENA managed at our tertiary center. Case 1: A 35-year-old male presented with unilateral nasal obstruction. Endoscopy and imaging revealed a hypervascular mass centered in the posterior ethmoid sinus, destroying the basal lamella and abutting the skull base. Histopathological analysis was initially confounded by features resembling a solitary fibrous tumor (SFT), requiring a comprehensive immunohistochemical panel (IHC) including STAT6 and nuclear beta-catenin to confirm the diagnosis of angiofibroma. Case 2: A 17-year-old male presented with symptoms and imaging (non-contrast CT) highly suggestive of a benign sphenochoanal polyp. An initial attempt at routine endoscopic removal was aborted due to unexpected, profuse hemorrhage. Subsequent advanced imaging (CTA/MRI) revealed a hypervascular sphenoid-based angiofibroma. Both patients underwent preoperative superselective embolization followed by successful, purely endoscopic tumor resection with no recurrence at 12 and 18-month follow-up, respectively. Conclusion: ENA is a critical, albeit rare, diagnostic consideration for any vascular sinonasal mass, regardless of patient age or tumor location. These cases underscore the unreliability of "classic" clinical and radiological signs, the diagnostic pitfalls of histopathological mimics like SFT and polyps, and the critical role of advanced IHC (nuclear beta-catenin) for definitive diagnosis. A modern, multidisciplinary approach combining preoperative embolization with endoscopic resection offers a safe and effective pathway to cure.

Maternal Hyperthyroidism and Delayed Diagnosis of Bilateral Choanal Atresia in a 4-Month-Old Infant: A Case Report on Stentless Endoscopic Reconstruction Meilia Ghinasari; Bestari Jaka Budiman; Dolly Irfandy
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 3 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i3.1543

Background: Bilateral choanal atresia (BCA) is a life-threatening congenital anomaly typically presenting as a neonatal respiratory emergency. Survival beyond the neonatal period without surgical intervention is exceptionally rare. While the etiology is multifactorial, emerging evidence implicates maternal thyroid dysregulation in craniofacial malformations. This study reports a rare case of BCA diagnosed in a 4-month-old infant and evaluates the efficacy of stentless endoscopic repair using laterally-based mucoperiosteal flaps. Case presentation: A 4-month-old female infant presented with failure to thrive (weight 5.2 kg, less than the 3rd percentile) and cyclical respiratory distress. Perinatal history revealed the mother had Graves' disease and discontinued methimazole at 6 weeks gestation. Retrospective analysis of maternal serum indicated uncontrolled thyrotoxicosis during the critical organogenesis window (TSH less than 0.01 mIU/L; fT4 2.8 ng/dL at 7 weeks). Diagnostic imaging confirmed mixed bony-membranous atresia. The patient underwent transnasal endoscopic choanoplasty using a laterally-based mucosal preservation technique. A 10-Fr silicone feeding tube was placed transnasally but did not function as a structural stent. Conclusion: The intervention resulted in immediate airway patency. Quantitative outcomes showed an increase in oxygen saturation from 96% to 99% on room air and significant weight gain from 5.2 kg to 6.7 kg over two months. Follow-up at six months showed no restenosis. This case suggests a potential dual-hit teratogenic mechanism involving early methimazole exposure and subsequent uncontrolled maternal hyperthyroidism. Furthermore, it supports the efficacy of stentless repair in minimizing granulation tissue formation.

Atypical Presentation of Clinically Non-Functioning Pituitary Macroadenoma: Case Report of Concurrent Graves' Disease and Multi-Axis Pituitary Hypofunction in a 40-Year-Old Female Rizkha Amaliya; Dolly Irfandy; Hesty Lidya Ningsih; Alexander Kam; Mardijas Efendi
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 6 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i6.1610

Background: Non-functioning pituitary adenomas (NFPAs) present variable clinical manifestations depending on tumor size and location. Concurrent primary autoimmune thyroid disease in NFPA patients is exceptionally rare and requires careful diagnostic distinction from secondary hypothyroidism. Case presentation: A 40-year-old female presented with progressive bilateral peripheral vision narrowing over 3 months, chronic bifrontal headaches, secondary amenorrhea, and nocturia-predominant polyuria. Neuro-ophthalmologic examination revealed bitemporal hemianopsia with visual acuity 20/200 left eye and 20/25 right eye. Endocrine evaluation demonstrated: primary hyperthyroidism (TSH 0.02 µIU/mL, FT4 28.32 pmol/L, positive thyroid receptor antibodies), secondary adrenal insufficiency (cortisol 1 µg/dL, ACTH <5 pg/mL), and hypogonadotropic hypogonadism (LH 1.62 IU/L). Neuroimaging revealed a 2.13 × 2.28 × 3.05 cm sellar/suprasellar lesion with optic chiasm compression (Knosp Grade II). Endoscopic transsphenoidal surgery achieved complete gross total resection. Histopathology confirmed null-cell adenoma with low Ki-67 proliferation index (2%). Conclusion: Postoperative course was uncomplicated with rapid visual field improvement, complete resolution of bitemporal hemianopsia by 2 weeks, and normalization of all endocrine axes by 12 weeks, including menstrual recovery and gonadal axis restoration. This case illustrates the diagnostic challenge of distinguishing secondary hyperprolactinemia from primary prolactinoma in NFPA, the rarity of concurrent Graves' disease with pituitary macroadenoma, and favorable outcomes with complete surgical decompression achieving multisystem endocrine recovery.

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