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Juniarti Juniarti
Jurusan Ilmu Tanah Fakultas Pertanian Universitas Andalas Padang
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Journal : medisains

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In vitro study: thymoquinone inhibits the proliferation and migration of keloid fibroblasts and increases their apoptosis Hadi, Restu Syamsul; Juniarti, Juniarti
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Early Release
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v0i0.23975

Abstract

Background: No treatment can eliminate keloids. Thymoquinone (TQ) is hypothesized to play a pivotal role in treating keloids by modulating cellular processes such as proliferation, migration, and apoptosis. However, the existing studies investigating its effects on these mechanisms in keloid fibroblasts are limited and require further exploration.Objective: This study aims to investigate the effects of TQ on the proliferation, migration, and apoptosis of keloid fibroblasts in vitro.Methods: This experimental study was conducted using keloid fibroblast cultured in vitro. Cells were seeded in a 96-well plate at a density of about 5x103 cells per well with 100 μl of culture medium, and cells were cultured for 24, 48, and 72 hr for each concentration of TQ. Cell proliferation was assessed using a CCK-8 Kit, measuring optical density with a microplate reader. Apoptosis was measured using the TUNEL assay. Cell migration following TQ treatment was evaluated using the Scratch assay. The statistical test used a one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) test. Results: TQ inhibited the proliferation of keloid fibroblasts at a dose of 5 µM after 48 hours of incubation and 10 µM after 24 hours of incubation. The inhibitory effect of TQ on fibroblast proliferation increased in a dose- and time-dependent manner. Treatment at 5 and 10 µM doses increased apoptosis in keloid fibroblast cultures. The TQ5 µM group achieved 60% closure, while the 10 µM group showed 55% closure. Migration was significantly inhibited in the 25 µM and 50 µM groups, with only 30% and 10% closure, respectively, at 72 hours.Conclusion: Thymoquinone inhibits the proliferation and migration of keloid fibroblast cells while promoting apoptosis. These properties suggest that TQ could be developed as a potential treatment for keloid-related skin issues.
Co-Authors . Reflinaldon A , Agustar. A M, Hendra A, Sari. Abdul Kahar Abdullah, Johari Yap Adrian Budiman Afdhal Muttaqin Afrima Sari Agil Syah Putra Agnes Andriyani Sentosa Agustian Agustian Akhmadi, Ali Alex Darmawan, Alex Almurni, Siti Amelinda Rosita Aminuyati Amrizal Saidi Anastasia Audina Candra Anastasya Debbie Aliantho Andi Sadapotto Angelina Evelyn Apandi, Aden Aries Novita Putri Aryenti, Aryenti Asdi Agustar Auzar Syarif Awang Al Hamdi Azhari, Rudi Azwar Rasyidin Bramanta, Giraldi Sapta Carolina Limantoro Caroline Janet Sukamto Christina Christina Christina Corolina Corolina Crystalita Crystalita Crystalita Damayanti, Ndaru Andri Devina Andriani Tandiono Devina Mustikarina Natanagara Dian Fiantis Doni Hariandi Dwita, Adel Karen Dyah Puspita Sari DYAH PUSPITA SARI Elisabeth Kartika Y.W Evelina Kurniawan Evelyn Stacia Evelyn Sutejo Evelyne Evelyne Evita Vianita Sari Fadil Hukama Hamdi Fajri, Riyadhul Fathan, Muhammad Fatma Andria Wahyuni Febriyani, Ellsa Indah Ferbiana Helena Ferdiansyah, Muhammad Aldy Ferry Aditya Fhigo, Lucky Fidelia Fidelia Fidelia Finda Yovita Finy Amelia Firdaus Hi Jahja Kunoli FITRI, RUHIL Florencia Irena Chandra Florensia Agatha Francisca Ivana Frans Sanjaya Fransisca Elsa Monica Frency Yunita Gabriela Natalia Lay Gladys, Asthya Gunadi Gunadi Gunadi Gunadi Gunadi Gunadi Gusnidar, G Hanani, Hulfiatun Hans William Santoso Harianti, Mimien Hasmiandy Hamid Hastin, Abiyyah Helena, Ferbiana Hendro, Junaidi Hermansah Hermansah, Hermansah Hermeria, Noveriza Hijri, Nurul Hikmawati Hikmawati Hukama, La Diadhan Ilham Wibowo Indra Dwipa Ineke Oktaviani Irfan Suliansyah Irwandi, Nurmayani Ivanovich, Vasenev Ivan Jannah, Anisa Raudhatul Jessica Kartika Halim Karina Novitasari Kathrin Angelin Hardy Khairati, Nurul Komalasari, Ulvi Intan Kurniati, Fimetha Riva Liana Dewi Lilyani Kartika Sinatra Lina Noersanti Lince, Lince Listiyani Chandra Lubis, Ira Handayani Lubis, Khadijah Margaret Carolina Maria Yunita Maya Genisa MD Yusoff, Bazli Bin Meisilva Erona Sitepu Monica Hendrik Monica Oktavia Musliar Kasim Mustika, Maya N, Sandi N, Sandi. Nabilah Nabilah Nadila Aulia Ardi Nadra, Nadra Nadya, Dea Nandita Samaralya Tori Nasution, Sarifa Ulfa Nathania Nathania Nelly Kawijaya Niken Wulandari Noferita, Jelsi Trisna Nofrita Sandi Novi Dwi Vivian Thejakusuma Novitasari, Karina Nunung Ainur Rahmah Nur Afni Nurfina Yenti Okianna Okianna Olgawati Hudoyo Ong Yuliana Pannya Pannya Pannya Pohan, Syahrial Hasanuddin Pratiwi, Nike Preztika Ayu Ardheta Puspa Sari, Puspa Ranti Novia Restu Syamsul Hadi Rika Kurniawati Risnah, Risnah Rizwan, Mohamad Rido Rosmilan Pulungan Ryan Budi Setiawan Sam Hermansyah Septaria, Vera Shinta Mulyana Simon Eric Hanjoyo Siska Effendi Siti Rahmah Stefany Sicilia Ahliawan Stephanie Loyanaputra Stephanie Natalie Tanzil Stephen Hadi Wibowo Sudewi Isminingsih Surya - Wibowo Sylvia Adriani T N, Novidra Thamrin, Nabila Pyrenina Tjamdinata, Wenny Vania Allan Sutanto Vera Septaria Veronica Wijaya Vincent Valentino Vivian Jacob Vonny Vinisia Wahyuni, Fatma Andria Waruwu , Ahmad Aswan Waruwu, Ahmad Aswan Wenny Tjamdinata Y Yuzirwan Yaherwandi Yuliana Yuliana Yulmira Yanti Yulnafatmawita Yulnafatmawita Yusuf Hartono Z, Naspendra Zain, Efendi Zainal Zawir Simon Zuldadan Naspendra