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All Journal Indonesian Journal of Pharmaceutical Science and Technology Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Berkala Kesehatan JMM (Jurnal Masyarakat Mandiri) Pharmacology and Clinical Pharmacy Research CARADDE: Jurnal Pengabdian Kepada Masyarakat Holistik Jurnal Kesehatan Journal of Midwifery and Nursing Jurnal Mantik Jurnal Ners Jurnal Ilmiah Farmako Bahari Science Midwifery Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Mandala Pengabdian Masyarakat Journal of Education Research Jurnal Sains dan Teknologi Farmasi Indonesia West Science Interdisciplinary Studies West Science Interdisciplinary Studies JI-KES (Jurnal Ilmu Kesehatan)
Diki Prayugo Wibowo
Sekolah Tinggi Farmasi Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Antibacterial Activity of Pluchea indica and Piper betle Ethanol Extract on Staphylococcus epidermidis and Pseudomonas aeruginosa Simanjuntak, Nela; Yuniarni, Umi; Prayugo, Diki
Pharmacology and Clinical Pharmacy Research Vol 1, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.448 KB) | DOI: 10.15416/pcpr.v1i2.15202

Abstract

Medicinal plants are widely used for the treatment of different infectious diseases. This study was aimed to investigate antibacterial activity of Pluchea indica (P. indica) and Piper betle (P.betle) ethanol extract on Staphylococcus epidermidis (S. epidermidis) and Pseudomonasaeruginosa (P. aeruginosa) using agar disk diffusion method. Ethanol extract of Piper betle showed more potent antibacterial activity than P. indica against S. Epidermidis with the highest inhibiton zone at 30.71 mm and 21.73 mm from 1 mg/ml concentration, respectively. In contrast, against P. aeruginosa, the ethanol extract of P. indica was more potent than P. betle with 21.44 mm and 20.12 mm of inhibition zone on 1 mg/ml concentration, respectively. There was no increased effect from the combination of these two extracts against these bacteria. When comparing the antibacterial activity of these extract with tetracycline asthe standard, we found that antibacterial activity of P. indica at the concentration of 0.9 mg/ ml was comparable with that of tetracycline at concentration of 12.52 μg/ml, while P. betle needed 0.3 mg/ml concentration to had similar activity with 10.51 μg/ml of tetracycline. In conlusion, the antibacterial activity of ethanol extracts of P. indica and P. betle indicated that these extract had sufficient potential to warrant further examination and development as a new antibacterial agent.Keywords: Pluchea indica, Piper betle, antibacterial, agar disk diffusion
Essential Oil Composition, Antioxidant and Antibacterial Activities of Nutmeg (Myristica fragrans Houtt) from Garut West Java Wibowo, Diki Prayugo; Febriana, Yessi; Riasari, Hesti; Auilifa, Diah Lia
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 3 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.125 KB) | DOI: 10.15416/ijpst.v5i3.16030

Abstract

West Java is rich in essential oil-producing plants, essential oils known to be responsible for some pharmacological activities among its antioxidant and antimicrobial. This research aims to know antimicrobial, antioxidant activity and identify the components of a compound essential oil of nutmeg (Myristica fragrans Houtt.). Components chemistry identified with GC-MS (Gas Chromatography-Mass pectrometer). Antioxidant activity measured by using the DPPH method (2 2-diphenyl-1-picrylhydrazyl) at 516 λ wavelength absorbance. Antimicrobial activity determined Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) using microdilution method. The result of GC-MS showed the highest component on nutmeg containing 22.22% myristicin. The antioxidant test showed nutmeg had IC50 at 3,16%, essential oil showed antibacterial activity against Gram positive and Gram negative bacteria. Minimum inhibitory concentrations of essential oils range from 0.313% to 10%. The content of essential oils of nutmeg plants that grow in the area of West Java Garut can be used to overcome the problems of antioxidants and antimicrobial.Key words: Antioxidant, Antibacterial, Pala (Myristica fragrans Houtt), Chemical Composition.
Essential Oil Composition, Antioxidant and Antibacterial Activities of Nutmeg (Myristica fragrans Houtt) from Garut West Java Diki Prayugo Wibowo; Yessi Febriana; Hesti Riasari; Diah Lia Auilifa
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 3 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.125 KB) | DOI: 10.24198/ijpst.v5i3.16030

Abstract

West Java is rich in essential oil-producing plants, essential oils known to be responsible for some pharmacological activities among its antioxidant and antimicrobial. This research aims to know antimicrobial, antioxidant activity and identify the components of a compound essential oil of nutmeg (Myristica fragrans Houtt.). Components chemistry identified with GC-MS (Gas Chromatography-Mass pectrometer). Antioxidant activity measured by using the DPPH method (2 2-diphenyl-1-picrylhydrazyl) at 516 λ wavelength absorbance. Antimicrobial activity determined Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) using microdilution method. The result of GC-MS showed the highest component on nutmeg containing 22.22% myristicin. The antioxidant test showed nutmeg had IC50 at 3,16%, essential oil showed antibacterial activity against Gram positive and Gram negative bacteria. Minimum inhibitory concentrations of essential oils range from 0.313% to 10%. The content of essential oils of nutmeg plants that grow in the area of West Java Garut can be used to overcome the problems of antioxidants and antimicrobial.Key words: Antioxidant, Antibacterial, Pala (Myristica fragrans Houtt), Chemical Composition.
Antibacterial Activity of Pluchea indica and Piper betle Ethanol Extract on Staphylococcus epidermidis and Pseudomonas aeruginosa Nela Simanjuntak; Umi Yuniarni; Diki Prayugo
Pharmacology and Clinical Pharmacy Research Vol 1, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.448 KB) | DOI: 10.15416/pcpr.v1i2.15202

Abstract

Medicinal plants are widely used for the treatment of different infectious diseases. This study was aimed to investigate antibacterial activity of Pluchea indica (P. indica) and Piper betle (P.betle) ethanol extract on Staphylococcus epidermidis (S. epidermidis) and Pseudomonasaeruginosa (P. aeruginosa) using agar disk diffusion method. Ethanol extract of Piper betle showed more potent antibacterial activity than P. indica against S. Epidermidis with the highest inhibiton zone at 30.71 mm and 21.73 mm from 1 mg/ml concentration, respectively. In contrast, against P. aeruginosa, the ethanol extract of P. indica was more potent than P. betle with 21.44 mm and 20.12 mm of inhibition zone on 1 mg/ml concentration, respectively. There was no increased effect from the combination of these two extracts against these bacteria. When comparing the antibacterial activity of these extract with tetracycline asthe standard, we found that antibacterial activity of P. indica at the concentration of 0.9 mg/ ml was comparable with that of tetracycline at concentration of 12.52 μg/ml, while P. betle needed 0.3 mg/ml concentration to had similar activity with 10.51 μg/ml of tetracycline. In conlusion, the antibacterial activity of ethanol extracts of P. indica and P. betle indicated that these extract had sufficient potential to warrant further examination and development as a new antibacterial agent.Keywords: Pluchea indica, Piper betle, antibacterial, agar disk diffusion
Lindungi Imunitas Masyarakat Dengan Minuman Herbal Siti Uswatun Hasanah; Diki Prayugo Wibowo; Diah Lia Aulifa
CARADDE: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2020): Desember
Publisher : Ilin Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31960/caradde.v3i2.602

Abstract

The aim of PKM is to increase public knowledge in an effort to increase the body's resistance of community members during the Covid 19 pandemic. The method of activity is to form a team of extension agents (women for Family Welfare Empowerment), accompanied by providing samples of herbal drink products and companion brochures. The results achieved from this activity, the community can get to know several medicinal plants that have immune-enhancing properties, along with a simple processing process.
KADAR TOTAL FLAVONOID PADA BERBAGAI VARIETAS BIJI KEDELAI (Glycine max) INDONESIA Siti Uswatun Hasanah; Diki Prayugo; Nitta Nurlita Sari
Jurnal Ilmiah Farmako Bahari Vol 10, No 2 (2019): Jurnal Ilmiah Farmako Bahari
Publisher : Fakultas MIPA Universitas Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.533 KB) | DOI: 10.52434/jfb.v10i2.654

Abstract

Kedelai merupakan salah satu komoditi tanaman pangan terbesar di Indonesia setelah padi dan jagung. Beberapa penelitian menunjukkan kandungan flavonoid dalam kedelai memberikan perubahan efek profilaktik pada pengobatan menggunakan kedelai, diantaranya menurangi resiko kanker prostat, kanker payudara,dan perkembangan kanker usus besar. Penelitian ini bertujuan untuk mengetahui kadar total flavonoid pada beberapa varietas biji kedelai serta mengetahui variaetas biji kedeai yang memiliki flavonoid tinggi. Penelitian ini dilakukan terhadap Sembilan varietas biji kedelai, yang diekstraksi dengan cara panas. Ekstrak yang dihasilkan dianalisis dengan menggunakan spektrofotometer, dan penentuan kadar total flavonoid didasarkan pada kurva kalibrasi kuersetin. Kadar total flavonoid pada Sembilan varietas biji kedelai bervriasi satu sama lain. Kadar total flavonoid tertinggi didapat dari biji kedelai varietas Argomulyo, yaitu 0,354 mg/gram biji kedeai kering. Kata kunci : Kedelai, varietas, flavonoid
KOMPOSISI KIMIA, AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI MINYAK ATSIRI AKAR WANGI (Vetiveria zizanoides L) Diki Prayugo Wibowo; Diah Lia Aulifa
Jurnal Ilmiah Farmako Bahari Vol 10, No 2 (2019): Jurnal Ilmiah Farmako Bahari
Publisher : Fakultas MIPA Universitas Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.699 KB) | DOI: 10.52434/jfb.v10i2.655

Abstract

Penelitian ini bertujuan untuk mengidentifikasi komponen senyawa dan mengetahui aktivitas antioksidan, antimikroba minyak atsiri Akar wangi (Vetiveria zizanoides. L). Minyak atsiri diperoleh dengan cara destilasi uap air dianalisis menggunakan kromatografi gas-spektrometri massa (KG-SM), aktivitas antioksidan ditentukan dengan metode DPPH serta aktivitas antibakteri ditentukan dengan metode mikrodilusi. Hasil analisis kromatografi gas-spektrometri massa (KG-SM) menunjukan adanya 43 senyawa dengan kandungan utama yaitu sikloisolongifolena (8,27%), 6-Isopropenil-4,8A-Dimetil-3,5,6,7,8,8A-Hexahidrol-1H-Naf (7,20%), delta cadinena (6,29%), aromedenedradiene (5,98%) dan alfa amorpena (5,45%). Aktivitas antioksidan menunjukkan bahwa minyak atsiri memiliki aktivitas antioksidan dengan IC50 0,515 μg. mL−1. Aktivitas antibakteri menunjukkan aktivitas terhadap bakteri uji Gram positif dan bakteri Gram negativ dengan konsentrasi hambat minimum minyak atsiri berkisar dari 62, 50 μg. mL−1 hingga 4000 μg. mL−1. Kandungan minyak atsiri dari tanaman akar wangi direkomendasikan untuk aktivitas antioksidan dan antibakteri. Kata kunci: Antioksidan, antibakteri, komposisi kimia, akar wangi (Vetiveria zizanoides)
KARAKTERISASI DAN AKTIVITAS REPELEN MINYAK ATSIRI SEREH WANGI (Cymbopogon nardus L), AKAR WANGI (Vetiveria zizanoides L.), NILAM (Pogestemon Cablin), CENGKEH (Syzgium aromaticum) ASAL KABUPATEN GARUT TERHADAP NYAMUK Aedes aegypti BETINA Diki Prayugo Wibowo; Ardi Rustamsyah; Yunan Kurniawan
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 13 No 2 Desember 2016
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.151 KB) | DOI: 10.31942/jiffk.v13i2.1702

Abstract

INTISARI Demam berdarah dengue (DBD) merupakan penyakit menular  yang hingga kini masih menjadi masalah, karena bisa menyebabkan kematian. Vektor utama penularan penyakit DBD adalah nyamuk Aedes aegypti.Salah satu cara menghindari nyamuk adalah dengan menggunakan repelen yang berasal dari minyak atsiri.  Tujuan penelitian ini adalah untuk mengkarakterisasi dan mengetahui aktivitasrepelen  minyak atsiri akar wangi (Vetiveria zizanoides  L.), cengkeh (Syzygium aromaticum (L.) Merr. & L.M.Perry), sereh wangi (Cymbopogon nardus L), dan nilam (Pogostemon cablin(Blanco) Benth.) dari kabupaten Garut terhadap nyamuk Aedes aegypti betina. Karakterisasi minyak atsiri meliputi penentuan warna, berat jenis, indek bias, kelarutan dalam etanol dan bilangan asam.   Pengujian  aktivitas repelen  dilakukan menggunakan lengan sukarelawan. Kontrol negatif menggunakan  virgin coconut oil  (VCO), sedangkan kontrol positif menggunakan N,N,-dietil-m-toluamida (DEET). Konsentrasi minyak atsiri yang digunakan dalam penelitian ini adalah  2,5%, 5%, dan 10%.  Pengamatan daya repelen dilakukan setiap 5menit sampai 15 menit dilakukan berulang  selama 3 kali. Sampel dinyatakan memiliki potensi repelen bila  potensi repelen  ≥95% .  Hasil penelitian  menunjukkan bahwa  minyak atsiri sereh wangi memiliki aktivitas repelen pada konsentrasi 10% dengan daya repelen 96.26%.   Kata kunci : repelen,  Aedes aegypti, minyak atsiri ABSTRACT Dengue hemorrhagic fever (DHF) is  an infectious disease that there are still be a problem, because it can cause death.Vector transmission of dengue fever is Aedes aegypti. One way to avoid mosquito is by using repellent  derived from the volatile oil.  The purpose of this research is to  characterized and  investigated of    repellent  activity  of    volatile oil akar wangiVetiveria zizanoides L.), cengkeh (Syzygium aromaticum  (L.) Merr. & L.M.Perry), sereh wangi (Cymbopogon nardus L), and nilam (Pogostemon cablin  (Blanco) Benth.)  from  Garut district against Aedes aegypti female. Characterization of volatile oils contain of determinations of colours, spesific gravity, refracty index, ethanol solubility and acid number. The repellent activity test was carried out using arms volunteers.Virgin coconut oil ( VCO)  was used as negative control, while control positive was  using n, n,-dietil-m-toluamida ( DEET ). Concentrations  of volatile oil used in  this  study were 2.5 %, 5 %, and 10 %.Observation of repellentheld every 5 minutes to 15 minutes with 3 times repeated. The sample has potent repellent activity if it has ≥ 95% potential repellent.  The result showed that the volatile oil of sereh wangi has repellent activity  with  96.26 % potential repellent in 10 % concentration. Keywords: repellent, Aedes aegypti, volatile oil
UJI ANTI BAKTERI OBAT KUMUR EKSTRAK ETANOL HERBA KEMANGI (Ocimum americanum L) TERHADAP Streptococcus mutans Siti - Sariyah; Diki Prayugo Wibowo; Sohadi - Warya
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 1, No 2 (2012)
Publisher : Sekolah Tinggi Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.188 KB) | DOI: 10.58327/jstfi.v1i2.17

Abstract

AbstrakTanaman kemangi (Ocimum americanum L) secara empiris diketahui mempunyai aktivitas dalam menghambat Streptococcus mutans yaitu bakteri yang selama ini dikenal sebagai penyebab karies gigi. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari suatu sediaan yang mengandung ekstrak etanol herba kemangi dalam bentuk sediaan obat kumur. Konsentrasi hambat minimum ekstrak kemangi adalah 0,3% b/v. Hasil formulasi sediaan obat kumur ekstrak kemangi memperlihatkan larutan berwarna coklat, beraroma mint dan kemangi, memiliki rasa manis dan mint. Sediaan obat kumur ekstrak kemangi mampu membunuh bakteri uji dengan waktu kontak 60 detik. Formula obat kumur ekstrak kemangi yang dibuat relatif stabil secara fisik selama 35 hari waktu pengamatan. Hasil pengujian aktivitas antibakteri menunjukkan bahwa formula yang mengandung ekstrak kemangi (1,25%), natrium benzoat (0,1%), alkohol 95% (0,5%), sorbitol (20%), gliserin (10%), aqua menthae-piperetae (0,5%), dan aquadest merupakan formula yang paling baik aktivitasnya. Kata Kunci : Obat kumur, Kemangi (Ocimum americanum L), Streptococcus mutans, Karies gigi.  AbstractKemangi herbs (Ocimum americanum L) empirically has inhibitory activity against the Streptococcus mutans bacteria that known to cause dental caries. This research aimed to inquire the antibacterial activity of a preparation containing ethanol extract of kemangi herbs in mouthwash dosage forms. Minimum Inhibitory Concentration of kemangi extract was 0,3% w/v. Performance of the gargle formula of kemangi extract showed the brown solution that has the odor of mint and "kemangi", and also taste of mint and sweet. Kemangi extract has a bactericide character against the Streptococcus mutans bacteria with a contact time of 60 seconds. The gargle formula physically stable during 35 days observation. The result showed that the antibacterial activity of the formula containing kemangi extract (1.25%), sodium benzoate (0.1%), alcohol 95% (0.5%), sorbitol (20%), glycerin (10%), aqua menthaepiperetae (0.5%), and aquadest is the best formula. Keywords: Gargle, Kemangi (Ocimum americanum L.), Streptococcus mutans, Dental caries.
UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI DAUN Ficus pubinervis DENGAN METODE MIKRODILUSI Feronia - Reni; Diki Prayugo Wibowo; Dewi - Astriany
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 5, No 2 (2016)
Publisher : Sekolah Tinggi Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.306 KB) | DOI: 10.58327/jstfi.v5i2.56

Abstract

AbstrakTelah dilakukan penelitian antibakteri terhadap ekstrak dan fraksi daun Ficus pubinervis dengan metode mikrodilusi. Hasil penelitian menunjukkan bahwa ekstrak etanol, fraksi n-heksan dan fraksi etil asetat daun Ficus pubinervis memiliki aktivitas antibakteri terhadap bakteri gram positif (Staphylococcus aureus, Bacillus cereus, dan Bacillus subtilis) serta bakteri gram negatif (Escherchia coli, Methicillin-resistant Staphylococcus aureus (MRSA), dan Pseudomonas aeruginosa) terkecuali fraksi air yang tidak menunjukkan aktivitas antibakteri. Kemudian dilakukan karakterisasi ekstrak dan fraksi dengan metode kromatografi lapis tipis (KLT). Ekstrak etanol daun Ficus pubinervis menunjukkan aktivitas antibakteri dengan Konsentrasi Hambat Minimum dan Konsentrasi Bunuh Minimum lebih dari 1000 mg/mL, sedangkan pada fraksi n-heksan dan fraksi etil asetat menunjukkan Konsentrasi Hambat Minimum lebih dari 1000 mg/mL.  Kata kunci : daun Ficus pubinervis, antibakteri, mikrodilusi, kromatografi lapis tipis  AbstractThere has been conducted research to determine antibacterial activity of extracts and fractions from Ficus pubinervis leaf using microdilution method. The research shows that ethanol extracts, n-hexane fraction and ethyl acetate fraction have antibacterial activity towards gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, and Bacillus subtilis) and gram negative bacteria (Escherchia coli, Methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa) but water fraction doesn’t showed any antibacterial activity. Extract and fractions of Ficus pubinervis were characterized by Thin Layer Chromatography (TLC) method. Ethanol extract of Ficus pubinervis leaf showed antibacterial activity with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were found more than 1000 mg/mL, while the n-hexane fraction and ethyl acetate fraction showed Minimum Inhibitory Concentration (MIC) more than 1000 mg/mL.  Keywords : Ficus pubinervis leaf, antibacterial, microdilution, thin layer chromatography
Co-Authors abdullah, vera iriani Ahmad Wahid Muharom Aldo, Novian Aldo, Novian Andarmoyo, Sulistyo Anggi Restiasari Ardi Rustamsyah Arifah Septiane Mukti Auilifa, Diah Lia Awang Sudrajat Baiq Fina Farlina Bambang Purwanto Budi Mardikawati Deby Tristiyanti Dewi Astriany Diah Afrianti Rahayu Diah Lia Auilifa Diah Lia Aulifa Dicki Bakhtiar Purkon Dwi Asa Verano Eddy Silamat Faizah Min Fadhlillah Febriana, Yessi Feronia - Reni Hasna Fauziah Nurgandi Hesti Riasari Indri Wulandari, Indri Irma Erika Herawati Irma Erika Herawati Irwan Ashari Komar Ruslan Wirasutisna Kraugusteeliana Kraugusteeliana Lea Ingne Reffita Mawaddah, Linda Rabbayani Miranti, Mila Muhamad Risal Tawil Muhammad Umar Kelibia Muntasir, Muntasir Najwa Najwa Nela Simanjuntak NFN Noviyanti Nitta Nurlita Sari Normila Normila Novriyanti Lubis Nurani Ai Erlinawati Perceka, Andhika Lungguh Perdana, Farid Rahifa Inayah Ria Mariani Ria Mariani Riska Prasetiawati Riski Dwi Prameswari Rival Ferdiansyah S, Irmawati Salma Annafiah Salma Firyal Fadhilah Sani Nurlaela Sani Nurlaela Fitriansyah Siti - Sariyah Siti Aminah Siti Rahma siti uswatun Siti Uswatun Hasanah Siti Uswatun Hasanah Sohadi - Warya Sulistyo Andarmoyo Sutriyawan, Agung Tanti Widia Nurdiani Teguh Arifianto Umi Yuniarni Wahidin Wahidin Wasngadiredja, Pupu Fujriani Wirasutisna, Komar R Yessi Febriana Yola Desnera Putri Yuliana Anggraeni Yunan Kurniawan Yusuf, Aldy Fadlillah