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All Journal Damianus Journal of Medicine MITRA: Jurnal Pemberdayaan Masyarakat Acta Biochimica Indonesiana Jurnal Muara Sains, Teknologi, Kedokteran dan Ilmu Kesehatan Jurnal Bakti Masyarakat Indonesia Jurnal Kesehatan Tambusai Devotion: Journal of Research and Community Service Prosiding Seri Seminar Nasional Jurnal Suara Pengabdian 45 KREATIF: Jurnal Pengabdian Masyarakat Nusantara Jurnal Sehat Indonesia (JUSINDO) Tarumanagara Medical Journal Perigel: Jurnal Penyuluhan Masyarakat Indonesia Jurnal Keperawatan Bunda Delima Jurnal Abdimas Indonesia Jurnal Muara Medika dan Psikologi Klinis Jurnal Pengabdian Masyarakat Nian Tana Panggung Kebaikan : Jurnal Pengabdian Sosial Cermin Dunia Kedokteran Jurnal Serina Abdimas KREATIF: Jurnal Pengabdian Masyarakat Nusantara
David Limanan
Bagian Biokimia Dan Biologi Molekuler Fakultas Kedokteran Universitas Tarumanagara, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Uji Kadar Fenolik Total dan Uji Kapasitas Antioksidan Daun Kunyit (Curcuma longa) dengan Metode ABTS Rumengan, Peterjohn Andrew Benhard; Limanan, David; Yulianti, Eny; Ferdinal, Frans
Jurnal Sehat Indonesia (JUSINDO) Vol. 7 No. 1 (2025): Jurnal Sehat Indonesia (JUSINDO)
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jsi.v7i01.170

Abstract

Bisphenol-A yang ditemukan pada beberapa galon minuman dapat menyebabkan ketidakseimbangan Reactive Oxygen Species (ROS) dengan meningkatkan mediator oksidatif dan menurunkan enzim antioksidan. Kadar ROS yang berlebihan memicu stres oksidatif, merusak makromolekul penting seperti lipid, protein, karbohidrat, dan asam nukleat, yang dapat menyebabkan penyakit metabolik dan degeneratif. Antioksidan memiliki peran penting dalam mencegah stres oksidatif dengan menghambat produksi ROS berlebih. Kunyit (Curcuma longa) merupakan tanaman herbal yang banyak digunakan di Indonesia, dengan daun yang diketahui kaya akan antioksidan dan fenolik. Penelitian ini bertujuan untuk menilai kapasitas antioksidan dan kadar fenolik total dari daun kunyit. Metode eksperimen yang digunakan adalah uji ABTS dan pengukuran kadar fenolik dengan asam galat. Ekstrak daun kunyit menunjukkan nilai IC50 sebesar 27,895 µg/mL dan kadar fenolik total sebesar 153,184 mgGAE/g. Uji ABTS menghasilkan nilai IC50 <50 µg/mL, yang menunjukkan kapasitas antioksidan yang sangat kuat. Temuan ini menunjukkan bahwa ekstrak daun kunyit memiliki potensi besar untuk dikembangkan sebagai pengobatan alternatif untuk penyakit metabolik dan degeneratif. Aktivitas antioksidan yang kuat dan kandungan fenolik tinggi menjadikan daun kunyit sumber alami yang menjanjikan untuk eksplorasi lebih lanjut dalam bidang pengobatan.
Konsentrasi Fenolik Total dan Aktivitas Antioksidan dari Ekstrak Metanol Daun Kari (Murraya koenigii L.): Hasil Penelitian Ardhita Felicia Tanuhariono; David Limanan; Frans Ferdinal; Eny Yulianti
Cermin Dunia Kedokteran Vol 52 No 5 (2025): Kardiologi
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v52i5.1333

Abstract

Introduction: Curry leaves (Murraya koenigii L.) are plants that naturally grow in the Indian subcontinent, except at the peak of the Himalayan mountains, which in their development have spread widely to various corners of the world. Traditionally, curry leaves have been used by people as medicine. However, it is now known that this plant contains carbazole alkaloid compounds and polyphenols that have antioxidant activity. This is very useful for controlling free radical levels in the human body so that they remain within reasonable limits. This is very useful for controlling the levels of free radicals in the human body to stay within reasonable limits. Free radicals are produced by normal cell metabolism which has recently been associated with the pathophysiology of various diseases. Methods: This in vitro research was to determine phenolic content and antioxidant potential in methanol extract of curry leaves. Total phenolic content testing used the Folin-Ciocalteu method and expressed by Gallic Acid Equivalent (GAE). Total antioxidant capacity testing used the DPPH method and Trolox as a comparison solution. Results: The total antioxidant capacity is expressed in the form of IC50. The total phenolic content of curry leaf methanol extract was 4007.78 μg/mL or equivalent to 133.59 mg GAE/g DW and the total antioxidant capacity of curry leaf methanol extract was 37.418 μg/mL. Conclusion: The total phenolic content of curry leaf methanol extract is high (>5 mg GAE/g). Correspondingly, the total antioxidant capacity of curry leaf methanol extract is also classified as very strong (<150 µg/mL).
Antioxidant activity of Crescentia cujete extract: effects on oxidative stress in the hearts of hypoxic rats Limanan, David; Setiady, Brandon Alexander; Sentosa, Belinda; Alphanto, Alfred H; Rudianti, Selly Herlia; Ferdinal, Frans
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.201

Abstract

Background: The heart is highly susceptible to oxidative stress. Hypoxia can induce oxidative stress in the heart, leading to cardiac damage. Objective: This study investigated the antioxidant effects of Crescentia cujete extract on oxidative stress in hypoxic rat hearts. Methods: Antioxidant capacity was evaluated using DPPH assay. Sprague Dawley rats were divided into eight groups: four received C. cujete extract (400 mg/kg/day for 14 days) and four served as controls. Hypoxia was induced for 3, 7, and 14 days using a hypoxic chamber (8% O₂, 92% N₂). Heart tissue was analyzed for malondialdehyde (MDA), glutathione (GSH), and catalase activity. Results: C. cujete extract demonstrated moderate antioxidant capacity (IC50 = 158.45 µg/mL). In extract-treated rats, MDA levels were significantly lower compared to controls, while catalase activity was significantly higher. GSH levels were higher in treated groups but not statistically significant. Histopathological analysis revealed less cardiac necrosis in extract-treated groups. Conclusion: C. cujete extract demonstrates protective effects against hypoxia-induced cardiac oxidative stress. These findings suggest potential as complementary therapy for oxidative cardiac damage, although further studies are needed to establish clinical efficacy and safety.
Correlation between Anthropometry and Fat Caliper Measurements with Triglyceride Ratios and Blood Pressure: Korelasi antara Pengukuran Antropometri dan Pengukuran Kaliper Lemak dengan Rasio Trigliserida dan Tekanan Darah Limanan, David; Santoso, Alexander; Teguh, Stanislas; Jap, Ayleen
Jurnal Keperawatan Bunda Delima Vol 7 No 2 (2025): EDISI AGUSTUS
Publisher : Akademi Keperawatan Bunda Delima Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59030/jkbd.v7i2.178

Abstract

Introduction: Anthropometric measurements, such as fat calipers and circumference, offer non-invasive insights into fat distribution and total adiposity, both crucial health markers. Increased visceral fat heightens cardiovascular risk by driving metabolic dysfunction. The TG/HDL-C ratio serves as a strong predictor of cardiovascular risk, linking systemic inflammation, dyslipidemia, and insulin resistance. Excess adiposity also elevates blood pressure by impairing lipid metabolism and vascular function. This study aims to see the correlation between anthropometry measurement (calf, neck, upper arm, and waist circumferences), body weight, body mass index and fat caliper with the triglyceride/HDL ratio and blood pressure. Methods: This cross-sectional study, conducted in Krendang Village in November 2024, included participants aged 18 years or older, excluding those using antihypertensive or cholesterol-lowering medications or unwilling to provide blood samples or undergo physical exams. Measurements included skinfold thickness at four sites, various anthropometric parameters, blood pressure, and venous blood samples analyzed for HDL, triglycerides, and their ratio. Statistical analysis utilized Spearman Correlation, focusing on the triglyceride/HDL ratio Results: Multivariate analysis revealed that waist circumference is the strongest predictor of the triglyceride/HDL ratio. Hip circumference and calf circumference were identified as key indicators for predicting both systolic and diastolic blood pressure, while suprailiac fat caliper and scapular fat caliper were specifically associated with predicting diastolic blood pressure.Conclusion:  Waist circumference, along with select anthropometric and fat caliper measurements, serves as valuable predictors for cardiovascular and metabolic risk factors, emphasizing their importance in early risk assessment.
Pelatihan Bantuan Hidup Dasar (BHD) untuk Meningkatkan Kesiapsiagaan Komunitas Karang Taruna di Kelurahan Kota Bambu, Jakarta Barat Sari Mariyati Dewi Nataprawira; David Limanan; Alexander Halim Santoso; Muhammad Fikri Dzakwan
JPMNT JURNAL PENGABDIAN MASYARAKAT NIAN TANA Vol. 3 No. 3 (2025): Juli: Jurnal Pengabdian Masyarakat Nian Tana
Publisher : Fakultas Ekonomi & Bisnis, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59603/jpmnt.v3i3.997

Abstract

Cardiac arrest is a critical medical emergency with a high mortality rate that requires immediate and appropriate action to increase the chances of survival. One key factor in improving survival outcomes is the involvement of bystanders, particularly young people, in providing Basic Life Support (BLS). This community service activity was carried out to improve the knowledge and practical skills of youth organization members (Karang Taruna) in Kota Bambu Subdistrict, West Jakarta, in recognizing and responding to cardiac arrest using BLS procedures. The activity adopted the PDCA (Plan-Do-Check-Act) approach. The planning stage involved the preparation of materials and training tools for both theoretical and practical learning. The implementation phase included a 45-minute health education session covering the basics of BLS, especially cardiopulmonary resuscitation (CPR), followed by hands-on training. Participants were divided into small groups and guided through practical CPR simulations using manikins, facilitated by six trained nursing students. The activity was conducted on June 14, 2025, and attended by 57 participants, with 27 actively participating in the CPR practice session. The evaluation stage revealed a notable increase in the participants’ understanding and ability to perform CPR effectively. This indicates that community-based BLS training can be a powerful tool in improving emergency response at the grassroots level. In addition to raising awareness, the activity also empowered the youth as first responders in their community, strengthening their role in emergency preparedness and response. The follow-up stage emphasized the importance of sustainability through continued collaboration between local health professionals and community organizations. In conclusion, BLS education and training programs for youth can significantly enhance community resilience and are recommended for broader implementation in other regions.
Strategi Preventif Penyakit Tidak Menular Melalui Edukasi dan Skrining Obesitas Dewasa Muda Jakarta Barat David Limanan; Susy Olivia
Panggung Kebaikan : Jurnal Pengabdian Sosial Vol. 2 No. 3 (2025): Agustus: Panggung Kebaikan : Jurnal Pengabdian Sosial
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/panggungkebaikan.v2i3.1914

Abstract

Obesity, central obesity, and hypercholesterolemia are prevalent yet often unrecognized health conditions that significantly elevate the risk for various non-communicable diseases (NCDs) such as diabetes mellitus, hypertension, and cardiovascular disorders. These conditions, when left undiagnosed and untreated, contribute to the growing public health burden worldwide. This community service program was designed to raise awareness and provide early detection of these conditions through basic health screening measures, including body mass index (BMI), waist circumference, and blood cholesterol levels. The program was implemented in Tomang Subdistrict, West Jakarta, where 90 participants were involved. The findings from the screening revealed concerning results: 67% of the participants were classified as obese, and 58% had central obesity, both of which are major risk factors for NCDs. A deeper analysis of lifestyle factors highlighted that high-fat diets, sedentary behaviors, and night shift work were significant contributors to the high rates of obesity and metabolic abnormalities observed. The educational outreach component of the program, which included the provision of information about healthy diets, exercise, and the importance of regular health screenings, proved to be effective in raising awareness about these health issues. Moreover, the simple health screening process was well-received by the community and contributed to a greater understanding of individual health risks. This initiative is expected to serve as a sustainable model for promoting health and preventing metabolic diseases in the community. The findings underscore the importance of early detection and education as critical strategies in the fight against obesity and related NCDs, suggesting that such programs can play a key role in reducing the future burden of chronic diseases in underserved populations
Uji Fenolik Total dan Kapasitas Antioksidan Ekstrak Buah Psophocarpus tetragonolobus Darma, Timothy Halomoan; Limanan, David; Yulianti, Eny
Jurnal Sehat Indonesia (JUSINDO) Vol. 7 No. 1 (2025): Jurnal Sehat Indonesia (JUSINDO)
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jsi.v7i01.159

Abstract

Reactive Oxygen Species (ROS) adalah molekul oksigen reaktif yang dihasilkan sebagai produk sampingan dari metabolisme sel normal, terutama di mitokondria. Meskipun ROS memainkan peran penting dalam proses fisiologis, akumulasi berlebih dapat menyebabkan kerusakan oksidatif pada biomolekul seperti DNA, protein, dan lipid. Antioksidan berfungsi untuk menetralkan ROS dan melindungi sel dari kerusakan oksidatif. Kecipir (Psophocarpus tetragonolobus) diketahui memiliki kapasitas antioksidan. Penelitian ini bertujuan untuk menganalisis komposisi kapasitas fenolik total dan kapasitas antioksidan keseluruhan dari ekstrak metanol yang berasal dari kecipir. Penelitian ini menggunakan desain studi eksperimental in vitro. Proses ekstraksi menggunakan metode maserasi dengan metanol sebagai pelarut. Analisis meliputi tes kapasitas antioksidan menggunakan 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) dan uji fenolik total. Uji kapasitas antioksidan menunjukkan nilai IC50 sebesar 47,7 µg/mL, mengklasifikasikannya sebagai antioksidan sangat kuat. Temuan ini menunjukkan potensi ekstrak kecipir sebagai antioksidan yang efektif. Sementara untuk uji fenolik totalnya 34,90 mg GAE/gram dengan pengenceran dua kali. Ekstrak metanol buah kecipir memiliki potensi sebagai sumber antioksidan alami yang efektif dengan aktivitas antioksidan yang kuat, serta kandungan fenolik yang tinggi, sehingga dapat dikembangkan lebih lanjut dalam aplikasi kesehatan, khususnya pencegahan penyakit akibat stres oksidatif.
Uji Kapasitas Antioksidan Pada Ekstrak Kayu Secang (Caesalpinia Sappan L.) Dengan Metode DPPH, FRAP Asyraf, Muhammad Zain Alwi; Limanan, David; Yulianti, Eny; Ferdinal, Frans
Jurnal Sehat Indonesia (JUSINDO) Vol. 7 No. 1 (2025): Jurnal Sehat Indonesia (JUSINDO)
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jsi.v7i01.165

Abstract

Antioksidan berperan sebagai pelindung dari kerusakan oksidatif, termasuk antioksidan eksogen yang bisa didapatkan dari bahan-bahan herbal. Salah satu contoh antioksidan eksogen alami yang banyak ditemukan di Indonesia adalah kayu secang (Caesalpinia sappan L.). Penelitian ini bertujuan untuk mengukur kapasitas antioksidan dari ekstrak kayu secang menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) dan FRAP (ferric reducing antioxidant power). Hasil uji kapasitas antioksidan dengan metode DPPH pada ekstrak kayu secang menunjukkan nilai IC50 sebesar 99,996 µg/mL, sementara dengan metode FRAP nilai IC50 yang diperoleh adalah 24,853 µg/mL. Berdasarkan nilai ini, kayu secang memiliki aktivitas antioksidan yang sangat kuat menurut metode FRAP (nilai IC50 ≤ 50 µg/mL) dan aktivitas antioksidan kuat menurut metode DPPH (nilai IC50 antara 50 – 100 µg/mL). Dengan demikian, kayu secang (Caesalpinia sappan L.) berpotensi dimanfaatkan sebagai antioksidan.
Uji Kadar Fenolik Total dan Uji Kapasitas Antioksidan Daun Kunyit (Curcuma longa) dengan Metode ABTS Rumengan, Peterjohn Andrew Benhard; Limanan, David; Yulianti, Eny; Ferdinal, Frans
Jurnal Sehat Indonesia (JUSINDO) Vol. 7 No. 1 (2025): Jurnal Sehat Indonesia (JUSINDO)
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jsi.v7i01.170

Abstract

Bisphenol-A yang ditemukan pada beberapa galon minuman dapat menyebabkan ketidakseimbangan Reactive Oxygen Species (ROS) dengan meningkatkan mediator oksidatif dan menurunkan enzim antioksidan. Kadar ROS yang berlebihan memicu stres oksidatif, merusak makromolekul penting seperti lipid, protein, karbohidrat, dan asam nukleat, yang dapat menyebabkan penyakit metabolik dan degeneratif. Antioksidan memiliki peran penting dalam mencegah stres oksidatif dengan menghambat produksi ROS berlebih. Kunyit (Curcuma longa) merupakan tanaman herbal yang banyak digunakan di Indonesia, dengan daun yang diketahui kaya akan antioksidan dan fenolik. Penelitian ini bertujuan untuk menilai kapasitas antioksidan dan kadar fenolik total dari daun kunyit. Metode eksperimen yang digunakan adalah uji ABTS dan pengukuran kadar fenolik dengan asam galat. Ekstrak daun kunyit menunjukkan nilai IC50 sebesar 27,895 µg/mL dan kadar fenolik total sebesar 153,184 mgGAE/g. Uji ABTS menghasilkan nilai IC50 <50 µg/mL, yang menunjukkan kapasitas antioksidan yang sangat kuat. Temuan ini menunjukkan bahwa ekstrak daun kunyit memiliki potensi besar untuk dikembangkan sebagai pengobatan alternatif untuk penyakit metabolik dan degeneratif. Aktivitas antioksidan yang kuat dan kandungan fenolik tinggi menjadikan daun kunyit sumber alami yang menjanjikan untuk eksplorasi lebih lanjut dalam bidang pengobatan.
Antioxidant Test, Total Alkaloid Content, Antimitosis Test and HPTLC Test of Chamomile Flower Extract (Matricaria Chamomilla L.) Tantowi, Ferdy; Limanan, David
Devotion : Journal of Research and Community Service Vol. 4 No. 1 (2023): Devotion: Journal of Research and Community Service
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/dev.v4i1.351

Abstract

Oxygen is one of the supports of multicellular life and is necessary for aerobic metabolism. However, the use of oxygen can produce by-products, one of which is reactive oxygen species (ROS) which can also increase due to external factors such as exposure to ionizing rays, and vehicle smoke. When reactive oxygen species levels are high, the body can experience a condition called oxidative stress, which is a condition that can cause tissue damage. To overcome this, antioxidants are needed, namely compounds that can donate electrons to free radicals to neutralize their effects. Antioxidants are divided into endogenous antioxidants and exogenous antioxidants. Endogenous antioxidants are antioxidants found in the body. Exogenous antioxidants are antioxidants that come from outside the body. Because there is an increase in ROS levels due to external influences, an increase in exogenous antioxidants is needed, one of which is chamomile flowers. This study evaluated qualitative phytochemical levels using the Harnborne method, total antioxidant capacity using the Blois method, total quantitative levels of alkaloids using the Trivedi et al method, BSLT toxicity using the Meyer method, and fingerprint analysis using HPTLC. The obtained chamomile flowers are dried and extracted by maceration using methanol solvent. The test results on chamomile flowers revealed phytochemical content in the form of alkaloids, betasianin, cardioglycosides, coumarins, flavonoids, phenols, quinones, saponins, steroids, tepenoids and tannins. Antioxidant ability 209.27 g/ml; Total alkaloid content of 12.62 g/ml; toxicity 174.39 g/mL; As well as fingerprint analysis chamomile flowers have active ingredients. Therefore, chamomile flowers can serve as a moderate antioxidant with antimitotic activity.
Co-Authors Akhmad, Fariz Azril Khaidar Alexander Halim Santoso Alexander Halim Santoso Alphanto, Alfred H Alya Dwiana Andrea Bianca Castafiore Kusuma Angela Aprilia Adinda Annisaa Nurrahma Ardyati Ardhita Felicia Tanuhariono Asyraf, Muhammad Zain Alwi Audina Leonita Clareta Vero Patricia Widya Darma, Timothy Halomoan Destianti , Erika E Efrany E Yulianti Edbert, Bruce Edwin Destra Eldy Eny Yulianti Eny Yulianti Eny Yulianti Eny Yulianti Eny Yulianti Eny Yulianti Eny Yulianti, Eny Erics Efrany Ezra, Pasuarja Jeranding F Wandy Farell Christian Gunaidi Felicia Tanuhariono, Ardhita Ferdian Ferdian Frans Ferdinal Frans Ferdinal Frans Ferdinal Frans Ferdinal Frans Ferdinal Fransiska Iriani Dewi Freddy Ciptono Gaofman, Brian Albert Gita Manerlin Kasihita Simatupang Grace Madeleine Halomoan Darma, Timothy Helmi Rizal Helmi Ivany Lius Pangestu Jap, Ayleen Jeffrey Saputra Kawi Jessica Geselda Salim Julianty, Eny Karinnia Karinnia Kevin Arya Lim Kusuma, Andrea Bianca Castafiore Lulu Lina Azzahrotin Fairuza M Lirendra Maharani, Ismi Ikrima Makarau, Erick Marcella E. Rumawas Marcella, Agnes Marcellino, Marcellino Maria Christina Dwiyanti Meilani Kumala Michael Chen Muhammad Fikri Dzakwan Natasha Olivia Christian Nawaika Shafira Putri Ngestinuari Salim NO Christian Putri, Nawaika Shafira R Benettan Rizky Putri Agustina Rudianti, Selly Herlia Rumawas, Marcella E Rumawas, Marcella E. Rumengan, Peterjohn Andrew Benhard Saerang, Stefanus Handy Salim, Melanie Santoso, Alexander Santoso, Alexander Halim Sari Mariyati Dewi Nataprawira Sentosa, Belinda Septiana, Devqa Nur Setiady, Brandon Alexander Shafira Putri, Nawaika Sidarta, Erick Stephanie Amadea Susy Olivia Susy Olivia Susy Olivia Lontoh Susy Olivia Lontoh Tantowi, Ferdy Tanuhariono, Ardhita Felicia Teguh, Stanislas Teguh, Stanislas Kotska Marvel Mayello Tenggono Son, Hadinata Timotius Timotius Tjahyanto, Teddy Tjie Haming Setiadi Triyana Sari Triyana Sari Wijayadi, Linda Julianti yanto, heri Yesan Suci Paramitha