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Cox-2 inhibition activities of creams containing anguilla bicolor and sea cucumbers extract on croton oil induced inflammation in mice Heru Sasongko; Waliyyin Razan Qanit; Raka Sukmabayu W.S; Agung Putu S.P.Kristyawan; Nining Sugihartini; Wisnu Kundarto; Dian Eka Ermawati
Pharmaciana Vol 10, No 3 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.015 KB) | DOI: 10.12928/pharmaciana.v10i3.14823

Abstract

The fatty acids, like EPA and DHA, were known as anti-inflammation. It works on inflammatory tissue, brought by edema plasma, and changed into resolvins, protectins, and maresins by the enzyme reaction. Anguilla bicolor and Sea cucumber fish are known to contain EPA and DHA. This study analyzes the inhibition Cox-2 effect of the combination of Anguilla bicolor oil with Sea cucumber extract as an active ingredient in a cream preparation. This experiment used 7 groups of male BALB/C strain mice: normal control; negative control; positive control; A. bicolor oil cream; H. leucospilota extract cream; a combination of A. bicolor : H. leucospilota (2:1) cream; a combination of A. bicolor : H. leucospilota (1:2) cream. The anti-inflammatory activity was evaluated by the amount of inflammatory cell, the thickness epidermis, and the amount of cell expression COX-2  on mice's back skin tissue induced by croton oil (0,1 %). After 3 days, histopathological skin tissues were made. The data were analyzed statistically by One Way ANOVA followed by LSD to know each group's differences at a significance level of 0.05. The experiment results showed that the formula with the best anti-inflammatory activities is the combination of A. bicolor and H. leucospilota (2:1) cream.  The decrease of the amount of inflammatory cell (75.97%), the thickness of the epidermis (43.88%), and the amount of COX-2 cell expressions (60.52%) from the formula did not differ significantly with the positive control (p>0,05). It can be concluded that A. bicolor and H. leucospilota have anti-inflammation activity based on the experiment results.
Optimization of Self-Micro Emulsifying Drug Delivery System) for Soursop Leaf (Annona muricata Linn.) Chloroform Extract Anif Nur Artanti; Fea Prihapsara; Dian Eka Ermawati; Aprilia Saefanan Shofa
Majalah Obat Tradisional Vol 25, No 2 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.51590

Abstract

Soursop leaf chloroform extract has anticancer activity.  The active ingredient of soursop leaf was acetogenin polypoid derivatives that have a lipophilic characteristic, and less effective to achieve action targets of drugs in biological systems. The Self-Micro Emulsifying Drug Delivery System (SMEDDS) was an effective drug delivery technique that increases the solubility of lipophilic drugs. This study aims to determine the proportion of optimum SMEDDS formula using Simplex Lattice Design (SLD) method. The Formula of SMEDDS was prepared using a combination of Tween 80-Croduret, Propylene Glycol, and Candlenut oil. Optimization formula with SLD method using Design-Expert software based on physical stability parameters there are the percent of transmittance and emulsification time. The optimum formula of SMEDDS was compared with SLD prediction formula using a statistical analysis t-test, then test of loading dose extract, stability test accelerated by centrifugation, particle size, and zeta potential. The proportion of optimum composition of Tween 80-Croduret, Propylene Glycol, and Candlenut oil of SMEDDS was 60.87%; 24.13%; 15.00% respectively. Results of transmittance 41.14±3.78% and emulsification time 119.0±2.08 seconds. The predicted SLD value for the transmittance percent was 55.0% and the emulsification time was 119.59 seconds. The result of the statistical analysis of one sample t-test showed no significant difference between observation results and SLD prediction. The SMEDDS system has F value of 0.99 and capable to load 25.0 mg chloroform extract of soursop leaf each system with an average particle size of 440 nm and zeta potential of +21.5 mV. 
Menjaga Kualitas Jamu Kunyit Asam Segar Industri Rumah Tangga X Yogyakarta dengan Penyimpanan Suhu yang Tepat Berdasarkan Kadar Kurkuminoid Dian Eka Ermawati; Villian Septiana; Wisnu Kundarto; Anif Nur Artanti; Sholichah Rohmani; Heru Sasongko
Jurnal Pengabdian Pada Masyarakat Vol 6 No 2 (2021)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202162.617

Abstract

IMPROVING THE QUALITY OF FRESH ACID TURMERIC JAMU OF HOME INDUSTRY X AT THE RIGHT TEMPERATURE STORAGE BASED ON CURCUMINOID CONCENTRATION. Tamarind turmeric herbal medicine contains about 10% curcuminoids, consisting of: 1-5% curcumin, demectosicurcumin and bisdemethoxy-curcumin. Curcuminoids are a group of phenolic compounds as antibacterial, anticholesterol and antioxidants. Home Industry (IRT) X in Yogyakarta produces fresh turmeric and sour herbal medicine and is sold via delivery via an online application. Handling and storage at inappropriate temperatures can affect curcuminoid levels in herbal medicine, so it is necessary to do research on curcuminoid levels at various temperatures to provide information on IRT X in particular and herbal medicine manufacturers in general. Samples of herbal turmeric acid IRT X Yogyakarta stored at room temperature (25±2°C), refrigerator temperature (4±2°C), and freezer temperature (-10±2°C), analyzed the levels of curcuminoids on variations in storage temperature manually. quantitative method with UV-Vis Spectrophotometry. The analysis begins with the manufacture of standard curves and assays which are replicated three times. The results showed that curcuminoid levels were affected by storage temperature. Samples of herbal turmeric acid IRT X Yogyakarta which were analyzed by UV-Vis spectrophotometry method obtained a standard curve of y = 0.009x + 0.025 with a value of r = 0.997. The results of curcuminoid levels at room temperature were 4.74±0.13; refrigerator temperature 4.85±0.22; and freezer temperature 5.18±0.22. The optimum temperature in the handling and storage of the tamarind turmeric herbal medicine is at low temperatures, namely the refrigerator temperature and freezer temperature to maintain the curcuminoid content in the herbal medicine.
Formulation of Anti-Acne Gel of Moringa oleifera, L. Ethanolic Extract and Antibacterial Test on Staphylococcus epidermidis Dian Eka Ermawati; Cahyarani Intan Ramadhani
Majalah Farmaseutik Vol 16, No 2 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3929.634 KB) | DOI: 10.22146/farmaseutik.v16i2.50319

Abstract

Moringa oleifera L. leaves contain flavonoid, alkaloid, and phenolic compounds which have antibacterial activity. Staphylococcus epidermidis is one of the bacteria that causes acne. The purpose of this study is to compare bacteriostatic ability of the extracts and gel for S. epidermidis bacteria and to get gel formula that can fulfills the physical properties of a good gel. Moringa leaves were extracted with maceration method using ethanol 70% in three days. HPMC 4000 was used as the polymer. Extract was added with variation concentrations of 5, 10, and 15%. Physical evaluation of gel was organoleptic, homogeneity, pH, viscosity, adhesion, and spread tests for 4 weeks. In vitro bacteriostatic activity test with 1% clindamycin gel as positive control and polymer gel as negative control. The result showed that variations concentrations of ethanolic extract of Moringa leaves affected the physical properties of gel including viscosity, pH, adhesion and spreadability. Bacteriostatic activity test of ethanolic extract of Moringa leaf was classified as strong activity, while 15% Moringa leaf ethanol extract gel was classified as moderate activity with average diameter was 9.14 mm
The Effect of Combination of Hydroxypropylmethylcellulose and Carboxymethylcellulose Sodium Polymers to The Physical Properties of Celery (Apium graveolens L.) Ethanolic Extract Patch Dian Eka Ermawati; Galuh Kartikasari
Majalah Farmaseutik Vol 16, No 2 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3338.559 KB) | DOI: 10.22146/farmaseutik.v16i2.50358

Abstract

Celery herbs (Apium graveolens L.) contain apigenin compounds (flavonoids) that are effective as antihypertensiv. Celery herbs used in the form of decoction and oral preparations have a deficiency that is voluminous and can cause gastric irritation. Patches were chosen because it can prevent gastric irritation and reduce the frequency of drug administration because the drug is delivered over the skin with a long duration. This study aimed to know the effect of variations in the ratio of the concentrations of hydroxypropyl methylcellulose (HPMC) and carboxymethylcellulose sodium (CMC sodium) polymers to the physical properties of celery herbs ethanol extract patch.This study was conducted with maceration of celery herbs using 96% ethanol and then dispersed in patch made with variations in the concentrations ratio of HPMC and CMC sodium polymers of 3:1, 1:1, and 1:3. Patches were tested for physical properties including organoleptic, pH, thickness, weight, folding endurance, and moisture content. Statistical analysis of test data used One Way ANOVA and Paired Samples T Test.The results of statistical analysis showed that the increase concentrations ratio of CMC sodium polymer in the combination of HPMC and CMC sodium polymers could increase the thickness, weight, and moisture content of celery herbs ethanol extract patch. The best patch was formula 2 with the same concentration ratio of HPMC and CMC sodium polymers because it showed the best results with a pH 6,12-6,29, thickness 1,37-1,53 mm, weighting 2,24-2,52 gram, folding endurance more than 300 times, and the moisture content was quite stable.  
Pengaruh Kecepatan Pencampuran Terhadap Sifat Fisik Lotion Nano-Ekstrak Etanol Kulit Buah Pisang (Musa balbisiana Colla) dan Daun Teh Hijau (Camellia sinensis L. Kuntze) Dian Eka Ermawati; Alfia Andhika Putri
Majalah Farmaseutik Vol 18, No 2 (2022)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v18i2.59209

Abstract

Kulit pisang kepok (M. balbisiana Colla) dan daun teh hijau (C. sinensis L. Kuntze) memiliki aktivitas antioksidan. Teh hijau mengandung katekin 20-30% meliputi epikatekin, epikatekin-3-galat, epigalokatekin, dan epigalokatekin-3-galat dengan nilai IC50 21,44 µg/mL. Kulit pisang kepok mengandung galokatekin, epikatekin, dan katekin. Aktivitas antioksidan pisang kepok yaitu 94,25% dengan nilai IC50 64,03±2,78 ppm yang tergolong sangat aktif. Pemakaian ekstrak sebagai zat aktif kosmetik memiliki pharmaceutical elegance rendah, sehingga ekstrak diformulasikan dalam SNEDDS (Self Nano-Emulsifying Drugs Delivery System). Maserasi kulit pisang kepok dan daun teh hijau menggunakan pelarut etanol 96%. Komponen formula SNEDDS (minyak kemiri : tween 80 : PEG 400) perbandingan 1:6:1; 1:7:1 dan 1:8:1. Orientasi ekstrak yang ditampung dalam sistem antara 100-500 mg. SNEDDS dengan loading ekstrak maksimal didispersikan dalam basis lotion kemudian dilakukan pecampuran dengan variasi kecepatan pencampuran (mortir, homogenizer kecepatan 500 dan 1000 rpm). Pengamatan sifat fisik dan kimia lotion meliputi viskositas, pH, daya sebar dan daya lekat. Analisa statistik menggunakan One Way Anova. Pengujian lotion dilakukan selama 12 hari dan data dianalisa menggunakan Paired T-Test. Hasil analisa statistik menunjukkan bahwa variasi kecepatan pencampuran berpengaruh signifikan terhadap daya sebar dan daya lekat, namun tidak berpengaruh terhadap viskositas dan pH lotion. Homogenizer dengan kecepatan 1000 rpm merupakan metode terbaik untuk lotion nano ekstrak etanol kombinasi kulit pisang kepok dan daun teh hijau karena memenuhi persyaratan sifat fisik dan kimia yang baik selama penyimpanan.
The Effect of Carrageenan Concentration on the Physical and Chemical Properties of Gummy Turmeric Acid Jamu Nurul Rochmawati; Dian Eka Ermawati
Majalah Farmaseutik Vol 17, No 2 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v17i2.63615

Abstract

Turmric acid Jamu is an Indonesian herbal drink that made from turmeric and tamarind. The herbal medicine industry in Sleman Yogyakarta produces Turmric acid Jamu, but the concentration of curcuminoid has not been determined. Consumers of Turmric acid Jamu are limited to women and adults. Children rarely want to consume jamu, even though the curcuminoid in turmeric can increase appetite, especially for todler. Innovation is needed to process Turmric acid Jamu into products that more desirable and efficacious. Gummy is made with the addition of a gelling agent so that the texture is chewy. Carrageenan is a gelling agent made from seaweed and is safe for food products. The purpose of this study was to determine the effect of variations in carrageenan concentrations on physical and chemical properties, also to determine the optimum concentration of carrageenan in gummy Turmric acid Jamu according to the Indonesian National Standard on soft candy. This research is an experimental laboratory with variations in the concentration of carrageenan (7.5%; 8.0%; 8.5%) and Turmric acid Jamu as the main ingredient. The test include organoleptic, pH, weight uniformity, water content, disintegration time and the respondent acceptance test. The concentration of curcuminoid using spectrophotometry UV-VIS method and statistical analysis. The results showed that carrageenan concentration affected organoleptic, weight uniformity, moisture content, and disintegration time, but did not affect pH. The curcuminoid concentration of Turmric acid Jamu was 1.47% and the gummy was 0.03% (w/w). The carrageenan concentration of 8.0% is the optimum formula because it meets the quality requirements of the gummy and is the most preferred by the respondents.
Sosialisasi Swamedikasi Tanaman Obat Keluarga (TOGA) dan Potensi Lingkungan Masyarakat di Desa Borongan Nindita Clourisa Amaris Susanto; Anif Nur Artanti; Dian Eka Ermawati
Madaniya Vol. 3 No. 3 (2022)
Publisher : Pusat Studi Bahasa dan Publikasi Ilmiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53696/27214834.244

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Desa Borongan merupakan salah satu desa yang terdapat di Kabupaten Boyolali. Desa tersebut mempunyai penduduk dengan perkarangan yang luas, daerah subur dan belum dimanfaatkan. Oleh karena itu, Desa Borongan ini mempunyai potensi dari segi lingkungan dan masyarakatnya untuk menjadi desa TOGA yang dapat dimanfaatkan sebagai obat mandiri keluarga atau dikembangkan menjadi produk yang bernilai ekonomis. Masyarakat masih belum menyadari khasiat TOGA terutama sebagai salah satu upaya swamedikasi dan menjaga imun tubuh terlebih di masa pandemi. Tujuan dari kegiatan ini adalah untuk memberikan pengetahuan kepada masyarakat agar tergerak untuk memanfaatkan lahan di rumah menjadi apotek hidup melalui penanaman TOGA dan pemanfaatanTOGA sebagai swamedikasi. Metode yang digunakan dalam kegiatan ini dimulai dengan sosialisasi pemberian materi, evaluasi dan pendampingan. Hasi dari kegiatan ini masyarakat lebih mengetahui manfaat TOGA sebagai swamedikasi dan dapat tergerak untuk menjadi desa TOGA yang dapat memberikan nilai ekonomis seperti menghasilkan produk dari TOGA.
Pelatihan Pemanfaatan Minyak Atsiri sebagai Bahan Aktif Balsam Aromaterapi kepada Warga Kelurahan Mojosongo M Fiqri Zulpadly; Dian Eka Ermawati; Heru Sasongko; Anif Nur Artanti; Sholichah Rohmani; Wisnu Kundarto; Diyah Tri Utami
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 4, No 4 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v4i4.6684

Abstract

Pandemi Covid-19 yang melanda hampir seluruh negara di dunia khususnya di Indonesia membuat banyak masyarakat cemas, stres, dan frustasi akibat ketidakpastian dari berbagai aspek kehidupan. Stres pada umumnya merupakan kondisi normal dan tidak menimbulkan masalah pada kesehatan. Balsam aromaterapi dengan bahan utama dari minyak atsiri dapat digunakan sebagai alternatif untuk dapat merelaksasikan tubuh serta berperan dalam pengaturan emosional. Pengabdian kepada masyarakat ini adalah transfer pengetahuan dan pendampingan pelatihan pembuatan balsam aromaterapi. Kegiatan yang dilakukan mencakup pemberian informasi terkait pembuatan balsam yang dilanjutkan dengan demo pelatihan pembuatan balsam aromaterapi. Kegiatan pengabdian yang dilaksanakan pada tanggal 25 Oktober 2022 menghasilkan sebuah produk balsam aromaterapi hasil karya tangan para warga yang turut hadir yang didampingi oleh tim dari D3 Farmasi UNS. Kegiatan ini telah mencapai sasaran yang diinginkan yaitu kemampuan warga Kelurahan Mojosongo dalam membuat suatu produk skala kecil menengah namun memiliki nilai yang tinggi baik dari aspek guna maupun finansial. Kegiatan pendampingan ini merupakan langkah awal dalam membentuk kemandirian masyarakat setempat untuk memproduksi balsam aromaterapi secara mandiri ataupun kelompok yang berada dalam lingkup UMKM.The Covid-19 pandemic that hit almost all countries, especially in Indonesia, made many people anxious, stressed, and frustrated due to the uncertainty of various aspects of life. Stress is generally a normal condition and does not cause health problems. Aromatherapy balms with the main ingredients of essential oils can be used as an alternative to relax the body and play a role in emotional regulation. This community service is the transfer of knowledge and assistance in training on making aromatherapy balm. The activities included providing information on making balm, followed by a training demonstration on aromatherapy balm. The service activity, carried out on October 25, 2022, resulted in an aromatherapy balm product that was handmade by the present residents, accompanied by a team from UNS Pharmacy D3. This activity has achieved the desired target, namely the ability of the residents of Mojosongo Village to make a small-medium-scale product that has a high value both in terms of use and finances. This mentoring activity is the first step in establishing the independence of the local community to produce aromatherapy balm independently or in groups within the scope of Micro Small and Medium Enterprises (MSME).
The effect of zinc oxide and Curcuma heyneana Val. combination on stability and sun protection factor (SPF) of lotion Dian Eka Ermawati; Dita Yuli Budiasih
Pharmaciana Vol 12, No 3 (2022): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.054 KB) | DOI: 10.12928/pharmaciana.v12i3.23380

Abstract

Indonesian people risk-averse effects of Ultraviolet (UV) exposure, so they need skin protection agents. Zinc Oxide (ZnO) is an effective UV reflection, but at a concentration of more than 10%, it can cause skin irritation. Combining ZnO with other UV protective agents is necessary, so its concentration can be reduced but still effective. The flavonoid in C. heyneana has potential as a sunscreen because chromophore groups can absorb UV B and UV A light. This study aims to examine the effect of the combination of ZnO and C. heyneana on the stability and SPF value of lotion. The lotion is made in five variations of the composition of ZnO and C. heyneana, F1 of 100: 0%; F2 of 0:100%; F3 of 50:50%; F4 of 25:75%; and F5 of 75:25% respectively. Test parameters include organoleptic, pH, spreadability, viscosity, and SPF. The test is recorded before and after the cycling test. Statistical analysis using Paired Sample T-Test. The combination of ZnO and C. heyneana affected organoleptic, viscosity, spreadability, and SPF value but did not affect pH. The higher concentration of ZnO will increase the viscosity, pH, and SPF but decrease the spreadability. The formula with a combination of ZnO and C. heyneana of 50%:50% is selected because it has an SPF value of 13.24 and; a viscosity of 48.67 d.Pas; pH of 7.1; and spreadability of 3.81 cm. 
Co-Authors Adi, Lutfan Prastyo Aditya, Shafira Nur Agung P. Surya Agung Putu S.P.Kristyawan Aini S., Aini Alfia Andhika Putri Amaris Susanto, Nindita Clourisa Andini, Bimar Putri Anggraini Febriyanty Anif Nur Artanti Anif Nur Artanti Anif Nur Artanti Anif Nur Artanti, Anif Nur Aprilia Saefanan Shofa Aratanti, Anif Nur Cahyarani Intan Ramadhani Cahyarani Intan Ramadhani Damayanti, Avina Kusuma Darojati, Ulfa Afrinurfadhilah Daswati Daswati Dita Yuli Budiasih Diyah Tri Utami Dyah Ayu Ambarwati Ediati Sasmito Esha ND., Pramitha Farida, Yeni Fea Prihapsara Fea Prihapsara Fea Prihapsara Fea Prihapsara, Fea Fitria, Krismona Rizky Nur Fridha Angelina Pamuji Galuh Kartikasari H., Muchammad Hanuriansyah, Yavi Hayunda I'zaaz Fajrin Heni Utami Prilantari Heru Sasongko Heru Sasongko Heru Sasongko Heru Sasongko Hidayati, Astri Husna, Hanna Miftahul Indah Purwantini Indrajati, Dyah Yekti KD, Anggi Kundarto, Wisnu Kuntari, Tabita Putri M Fiqri Zulpadly M. Fiqri Zulpadly M. Fiqri Zulphadly M. Nur Dewi Kartikasari M. Nur Dewi Kartikasari Meitasari, Annisa Diyah Meitasari, Annisa Diyan Muchammad H., Muchammad mufrod mufrod Nailul Maghfiroh ND., Pramitha Esha Niken Rosyana Dewi Septini Nindita Clourisa Amaris Susanto Nining Sugihartini Noveri, Priscilla Novi Andriani Novi Andriani, Novi Nugraha Mas'ud Nurul Rochmawati Parwatiningsih, Sri Anggarini Putri, Hapsari Duanda Putriana, Susanti Putro, David Sarono Putu UA., Ni Qutratu'ain, Syahla Rahmat S, Anang Kuncoro Raka Sukmabayu W.S Ramadhani, Cahyarani Intan Rochmani, Sholichah S., Aini Saifullah Sulaiman Salsabila, Fitri Samrotul Jannah Setyowati, Tri Sholichah Rohmani Sholichah Rohmani Sholichah Rohmani Sholichah Rohmani Sholichah Rohmani, Sholichah Sulaiman, TeukuNanda Saifullah Susanti, Indhun Dyah Susanto, Nindita Claurisa Amaris Susanto, Nindita Clourisa Amaris Sutarno Sutarno Suwaldi . Suwaldi Martodiharjo T.N Saifullah Sulaiman Tambunan, Rido Rohana UA., Ni Putu Ul Millah, Nida untari, meta kartika Utami, Diyah Tri Villian Septiana Waliyyin Razan Qanit Wisnu Kundarto Wisnu Kundarto Wisnu Kundarto Wisnu Kundarto Wulandari, Wening Yeni Farida Zulpadly, M. Fiqri Zulpadly, M.Fiqri Zulpadly, Muhammad Fiqri Zulphadly, M.Fiqri